Altered microRNA expression in patients with non-obstructive azoospermia
- Jie Lian†1,
- Xiansheng Zhang†2,
- Hui Tian1,
- Ning Liang1,
- Yong Wang1,
- Chaozhao Liang2,
- Xin Li3 and
- Fei Sun1Email author
© Lian et al; licensee BioMed Central Ltd. 2009
Received: 05 November 2008
Accepted: 11 February 2009
Published: 11 February 2009
MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility.
In this study, the miRNA expression profiles of testes of patients with non-obstructive azoospermia (NOA) and normal controls were performed by using microarray technologies.
Altered microRNA expression in NOA patients was found, with 154 differentially down-regulated and 19 up-regulated miRNAs. These findings have been confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) assays on select miRNAs, including miR-302a, miR-491-3p, miR-520d-3p and miR-383. Several down-regulated miRNA clusters in patients with NOA were identified, such as the oncogenic potential of the mir-17-92 cluster and mir-371,2,3 cluster.
This is the first report that the expression of miRNAs is altered in testicular tissues of patients with NOA, suggesting a role of miRNAs in regulating spermatogenesis in human males.
Infertility is a worldwide reproductive health problem which affects 10%–15% of couples. Half of the cases are due to male factors, and about 60–75% of male infertility cases are idiopathic, since the molecular mechanisms underlying the defects remain unknown . A significant proportion of idiopathic male infertility is accompanied by severe oligozoospermia or azoospermia. Spermatogenic cells are characterized with stringently regulated spatiotemporal gene expression and strongly repressed translation in meiotic and haploid male germ cells. For example, impaired chromosome synapsis (marked by synaptonemal complex protein 3 (SCP3) and SCP1) and decreased meiotic recombination (marked by human mutL homologue 1, MLH1, an ortholog of the Escherichia coli Mut L mismatch repair protein) [2–4], were identified in infertile individuals with non-obstructive azoospermia (NOA). Such meiotic errors make these cells susceptible to spermatogenetic arrest and the production of aneuploid gametes. However, the molecular pathways of genetic defects in spermatogenesis are not known. Recently, the mouse maelstrom homolog (MAEL) protein was found in unsynapsed chromosomes of the spermatocyte nucleus and in the chromatoid body (a site where RNA and RNA processing enzymes accumulate) . Furthermore, MAEL interacts with the microRNA (miRNA) pathway-associated proteins mouse vasa homolog (MVH, a germ cell specific RNA helicase), piwi-like homolog 2 (MILI) and piwi-like homolog 1 (MIWI) (Argonaute family members). These observations suggest that miRNAs may be involved in translational repression of meiotic synapsis during spermatogenesis; if so, aberrant miRNA expression would be associated with male infertility.
MicroRNAs are a family of small non-coding RNAs (typically 19–23 nt), which play important roles in regulating post-transcriptional gene silencing through base-pair binding to their target messenger RNAs (mRNAs) . Numerous miRNAs are exclusively or preferentially expressed in the mouse testis , suggesting that miRNAs may play important roles in spermatogenesis. A role for miRNAs in translational repression during spermatogenesis has been proposed, which shows that components of the miRNA biogenetic pathway are highly concentrated in chromatoid bodies [8, 9]. Transition protein 2 (Tnp2), a post-transcriptionally regulated testis-specific gene in postmeiotic germ cells, was found to be regulated by miR-122a . In Dicer-deleted testis, spermatogenesis was retarded at an early stage of proliferation and/or early differentiation . Thus, it is reasonable to speculate that miRNAs may be involved in meiotic gene silencing, and that alteration in miRNA expression may be a factor in male infertility. In this study, the expression profiles of miRNAs in the testes of men with NOA and controls were first examined by miRNA microarrays, and the differences in expression levels were verified by quantitative RT-PCR for some of the differentially expressed miRNAs identified. The potential mRNA targets of the miRNAs were predicted using a bioinformatics approach.
Sample collection and RNA extraction
Testicular samples were obtained from the First Affiliated Hospital of Anhui Medical University (Hefei, China). Three patients (ages 22–30 years) with NOA and two patients (ages 60–63 years) undergoing orchiectomy for prostate carcinoma (controls) were recruited for this study. The testicular histology of the NOA patients showed a global early maturation arrest pattern. Two semen analyses indicated azoospermia. An ideal study population as normal controls would consist of volunteers of known fertility, but difficulties in acquiring testicular samples makes this impractical. Instead, samples were analyzed from urology patients who had no history of meiotic defects or infertility and histological examination showed normal spermatogenesis. In addition, none of the controls were exposed to adjuvant hormonal therapy prior to orchiectomy. Informed consent was obtained from all patients, and this study received ethical approval from the institutional review boards of the University of Science and Technology of China and the Anhui Medical University.
Immediately after retrieval, testicular tissues were snap-frozen in liquid nitrogen and stored at -80°C until processed. Total RNA was isolated using Trizol reagent (Invitrogen, USA), following the manufacturer's protocol. The quality of extracted RNA was measured using UV absorbance and denaturing agarose gel electrophoresis.
MicroRNA microarray analysis
MicroRNA expression profiles of testicular tissues from NOA patients and controls were generated by applying the miRCURY Locked Nucleic Acid (LNA) microarray platform (Exiqon, Denmark). All procedures were carried out according to manufacturer's protocol. Briefly, 1 μg total RNA was dual-labelled with dyes spectrally equivalent to the Cy3™ and Cy5™ fluorophores, using a miRCURY™ Array Power Labelling kit (Exiqon, Denmark). Labelled miRNAs were used for hybridization on a miRCURY™ LNA microRNA Array containing Tm-normalized capture probes for 582 human miRNAs. Microarrays with labelled samples were hybridized at 56°C for overnight using a heat-shrunk hybridization bag (Phalanx Hybridization Assembly, Phalanx Biotech, Taiwan, China) and washed using miRCURY™ Array Wash buffer kit (Exiqon, Denmark).
Slides were scanned using a Genepix 4000B laser scanner (Axon Instruments, USA) and microarray images were analyzed using Genepix Pro 6.0 software (Axon Instruments, USA). The patients with NOA group and the patients undergoing orchiectomy for prostate carcinoma group, were pooled to represent the study and the control group. Differentially expressed miRNAs were defined as genes whose expression in the study group is consistently altered two-fold (either greater or less) compared to the control group. The 2-fold cut-off is a default for many microarray experiments because it can reflect the variability in the population of samples. Hierarchical clustering for differentially expressed miRNAs was generated by using standard correlation as a measure of similarity.
Genomic coordinates were determined for each miRNA, and mapped to a specific chromosomal region in the human genomes using miRBase: Sequence Release 10.1 [12, 13] combined with the UCSC Genome Browser . Patterns of miRNA gene clustering and chromosomal distribution of differentially expressed miRNAs in the human genome were also determined.
To better understand the function of miRNAs, putative mRNA targets of differentially expressed miRNAs were predicted by four algorithms: miRBase Targets , TargetScan , DIANA-microT  and PicTar . The intersections of the four algorithms were obtained from miRGen .
Real-time quantitative PCR
Oligonucleotides used in this study.
Primer set name
Reverse transcriptase reaction primer (5' to 3')
Real-time quantitative PCR primer (5' to 3')
To investigate the cell-specific distribution of miRNA in normal and NOA tissues, in situ hybridizations were performed using 5'end digoxigenin (DIG)-labelled LNA modified DNA oligonucleotides (LNAs) complementary to the mature miRNA supplied by Exiqon A/S (Denmark). In this study, the expression of miR-383 was examined in testicular tissues. LNAs had the following sequences: LNA-miR-383, 5'-agccacaatcaccttctgatct-3'. Furthermore, LNA-scrambled, 5'-gtgtaacacgtctatacgccca-3' serves as negative control.
In situ hybridization in human testis tissue was performed essentially as described by Obernosterer et al.(2007) . After fixation with 4% paraformaldehyde for 15 min, 10 μm testis sections were immersed and stirred gently in 0.1 M ethanolamine and 2.5% acetic anhydride for 10 min to block endogenous alkaline phosphatase activity. The slides were treated with 5 μg/ml proteinase K for 3 min, followed by extensive washing with PBS. Pre-hybridizations were performed for 6 hr in hybridization oven at a temperature of 52°C with 700 μl pre-hybridization buffer (50% formamide, 5 × SSC, 5 × Denhardt's, 200 μg/ml yeast RNA, 500 μg/ml salmon sperm DNA, 2% Roche blocking reagents and DEPC-treated water). 1 pmol LNA probe was added to 150 μl denaturizing hybridization buffer (50% formamide, 5 × SSC, 5 × Denhardt's, 200 μg/ml yeast RNA, 500 μg/ml salmon sperm DNA, 2% Roche blocking reagents, 0.25% CHAPS, 0.1% tween and DEPC-treated water). After denatured by heating up to 80°C for 5 min and then quickly placed on ice, the probe mixture was pipetted onto the tissues. Glass coverslips were applied, and the slides were hybridized overnight at the pre-hybridization temperature. Sections were soaked in pre-warmed 60°C 5 × SSC to remove the coverslips, and incubated in 0.2 × SSC at 60°C for 1 h. Slides were then incubated in B1 solution (0.1 M Tris pH 7.5/0.15 M NaCl) at room temperature for 10 min. After pretreated in 20% sheep serum (Santa Cruz, USA) diluted with B1 solution at room temperature for 1 hr, the sections were incubated overnight at 4°C in 10% sheep serum containing anti-Digoxigenin-AP FAB fragments (Roche, USA; 1:250). Sections were then washed three times for 5 min in B1 solution at room temperature and equilibrated for 10 min in B3 solution (0.1 M Tris pH 9.5/0.1 M NaCl/50 mM MgCl2). Staining with NBT/BCIP (Roche, USA) was done overnight at room temperature. When each probe had yielded a strong signal, or until the negative control began to show background label, reactions were stopped by washing the slides for 3 × 10 min in 1 × PBS. Finally, the slides were mounted with the aqueous mounting medium sealed with nail polish. Signals were visualized under a standard light microscopy.
Differential expression of miRNAs between normal and NOA testes
Summary of down-regulated miRNAs that appear to be the testicular miRNAs†
Genomic location of differentially expressed miRNAs
Clusters of miRNAs within the human genome were also identified. Expressions of 12 of 13 members of a miRNA cluster located at human chromosome 14q32.31 were down-regulated in infertile men, with only miR-654-5p up-regulated (See additional file 3: Table S3 for Several differentially expressed miRNA clusters in NOA patients.). Two miRNA clusters located at chromosome 19q13.42 were identified. One is the mir-371,2,3 cluster, consisting of differentially down-regulated miR-372, miR-373 and up-regulated miR-371-5p in patients with NOA; the other cluster, of 13 differentially down-regulated miRNAs (See additional file 3: Table S3 for Several differentially expressed miRNA clusters in NOA patients.), was reported as a new cluster of non-conserved miRNAs . Of particular note is that several members of the mir-17-92 family occur relatively infrequently in NOA patients. These miRNAs are encoded by three paralogous clusters located at chromosome 13q31.3 (the mir-17 cluster, containing miR-18a*, miR-19a, miR-19b-1* and miR-92-1), chromosome Xq26.2 (the mir-106a cluster, containing miR-18b*, miR-20b and miR-92-2) and chromosome 7q22.1 (the mir-106b cluster, containing miR-93 and miR-93*) (Fig. 4B) .
Putative miRNA target gene prediction
Potential miRNA targets† that are differentially expressed in infertile testis††
Potential miRNA targets
Tissue inhibitor of metalloproteinase 3 (TIMP3)
SRY (sex determining region Y)-box 9 (SOX9)
Growth arrest and DNA-damage-inducible, gamma (GADD45G)
The meiotic and haploid phases of spermatogenesis are characterized by high transcriptional activity but suppressed translational activity. Post-transcriptional control of gene expression in these phases can be mediated by sequences in the 5' and 3'-untranslated regions (UTRs) of mRNAs where they may be regulated by miRNAs, indicating that miRNAs might play important roles in spermatogenesis . In the current study, using the microarray assay, altered expression of miRNAs in the testes of patients with NOA was identified for the first time, suggesting that aberrant miRNA expression may contribute to spermatogenesis arrest in human males.
Many clusters of miRNAs have been identified within the human genome [22, 26]. One of the largest miRNA clusters identified to date is hsa-miR-127, a cluster comprised of greater than 50 members residing on an imprinted region of human chromosome 14q32 . Another cluster on chromosome 19 located at positions 58861745–58961404 (HG17), is the largest non-conserved miRNA cluster ever reported and is comprised of 54 miRNAs . Moreover, almost all the known miRNAs encoded by genes on the X-chromosome are expressed in the testis [7, 22]. In the present study, preferential down-regulation of miRNAs in chromosomes 14, 19 and X was found in NOA patient testicular tissues. These findings suggest that expression of miRNAs in regions of 14q32.31, 19q13.42 and the X chromosome could be important in spermatogenesis.
Among the differentially expressed miRNAs, it is interesting to note that several members of the mir-17-92 cluster and mir-371,2,3 cluster are downregulated in NOA testicular tissue. These miRNAs are potential novel oncogenes participating in the development of human testicular germ cell tumors [27, 28]. The mir-17-92 cluster is highly expressed in carcinoma in situ (CIS) testis and inhibits apoptosis by translationally down-regulating E2F transcription factor 1(E2F1) in CIS cells . The expression of miR-372 and miR-373 permits proliferation and tumorigenesis of primary human cells by harboring both oncogenic RAS and active wild-type p53 . Therefore, mir-17-92 cluster and miR-372/miR-373 may down-regulate apoptosis genes and potentially have a role in inhibiting apoptosis. In this study, low expression of these miRNAs was found in NOA patients, which may contribute to increased apoptosis in the testes of patients with NOA .
The prediction of putative mRNA targets for differentially expressed miRNAs will help to characterize the miRNAs involved in biological processes. For example, three possible target genes for down-regulated miRNAs, TIMP3, SOX9 and GADD45G, were significantly increased in infertile testis . TIMP-3, a potential target of four down-regulated miRNAs (miR-1, miR-181a, miR-221 and miR-9*), is thought to play an active role in testicular development and differentiation . The transcription factor SOX9, a putative target of miR-145, is required for Sertoli cell maturation and normal spermatogenesis . As a potential target of miR-383, GADD45G can induce apoptosis and inhibit cell growth in response to stress shock . Abnormal expressions of these proteins may have a significant impact on male infertility.
During meiotic prophase I of spermatogenesis, synapsis of homologous chromosomes and recombination are essential for the formation of haploid spermatozoa [33, 34]. Impaired chromosome synapsis and decreased meiotic recombination can lead to meiotic arrest in spermatogenesis [2, 4, 35]. In this study, the four differentially expressed miRNAs were chosen to be confirmed in NOA patients by quantitative RT-PCR due to their potential role in regulating meiotic recombination- and synapsis-related genes. For example, one of the predicted target genes of miR-302a and miR-383 is MLH1, while miR-491-3p and miR-520d-3p is SCP1 and SCP3, respectively. The miR-383 was chosen for further in situ hybridization analysis because the relative abundance of this miRNA was found be in meiotic prophase . Whether the altered patterns of miRNA expression contribute to defects in chromosome synapsis and recombination in NOA patients awaits further study.
In summary, miRNA expression in testes was profiled and results were compared between patients with NOA and control men. Several miRNA clusters were also examined. However, the cellular origins of testicular miRNAs remain unknown. Azoospermic men with testicular failure have different problems ranging from Sertoli cell-only pattern to maturation arrest, or hypospermatogenesis. Therefore, analysis of the cellular origins and the target genes of these differentially expressed miRNAs in even larger populations of infertile men is warranted in order to shed light on their regulatory roles in spermatogenesis and male infertility.
We would like to thank Evelyn Ko from the University of Calgary, Canada for editing the paper. The research was funded by the National Natural Science Foundation of China (No. 30770811 to F.S.); the National Basic Research Program of China (Project No. 2007CB947400, 2009CB941700 to F.S.); a grant from the Chinese Academy of Sciences (No. KSCX1-YW-R-51 to F.S).
- Okada H, Tajima A, Shichiri K, Tanaka A, Tanaka K, Inoue I: Genome-Wide Expression of Azoospermia Testes Demonstrates a Specific Profile and Implicates ART3 in Genetic Susceptibility. PLoS Genet. 2008, 4 (2): e26-10.1371/journal.pgen.0040026.PubMed CentralView ArticlePubMedGoogle Scholar
- Sun F, Turek P, Greene C, Ko E, Rademaker A, Martin RH: Abnormal progression through meiosis in men with nonobstructive azoospermia. Fertil Steril. 2007, 87 (3): 565-571. 10.1016/j.fertnstert.2006.07.1531.View ArticlePubMedGoogle Scholar
- Sun F, Trpkov K, Rademaker A, Ko E, Martin RH: Variation in meiotic recombination frequencies among human males. Hum Genet. 2005, 116 (3): 172-178. 10.1007/s00439-004-1215-6.View ArticlePubMedGoogle Scholar
- Sun F, Kozak G, Scott S, Trpkov K, Ko E, Mikhaail-Philips M, Bestor TH, Moens P, Martin RH: Meiotic defects in a man with non-obstructive azoospermia: case report. Hum Reprod. 2004, 19 (8): 1770-1773. 10.1093/humrep/deh335.View ArticlePubMedGoogle Scholar
- Costa Y, Speed RM, Gautier P, Semple CA, Maratou K, Turner JM, Cooke HJ: Mouse MAELSTROM: the link between meiotic silencing of unsynapsed chromatin and microRNA pathway?. Hum Mol Genet. 2006, 15 (15): 2324-2334. 10.1093/hmg/ddl158.View ArticlePubMedGoogle Scholar
- Stark A, Bushati N, Jan CH, Kheradpour P, Hodges E, Brennecke J, Bartel DP, Cohen SM, Kellis M: A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands. Genes Dev. 2008, 22 (1): 8-13. 10.1101/gad.1613108.PubMed CentralView ArticlePubMedGoogle Scholar
- Ro S, Park C, Sanders KM, McCarrey JR, Yan W: Cloning and expression profiling of testis-expressed microRNAs. Dev Biol. 2007, 311 (2): 592-602. 10.1016/j.ydbio.2007.09.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Kotaja N, Bhattacharyya SN, Jaskiewicz L, Kimmins S, Parvinen M, Filipowicz W, Sassone-Corsi P: The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components. Proc Natl Acad Sci USA. 2006, 103 (8): 2647-2652. 10.1073/pnas.0509333103.PubMed CentralView ArticlePubMedGoogle Scholar
- Kotaja N, Sassone-Corsi P: The chromatoid body: a germ-cell-specific RNA-processing centre. Nat Rev Mol Cell Biol. 2007, 8 (1): 85-90. 10.1038/nrm2081.View ArticlePubMedGoogle Scholar
- Yu Z, Raabe T, Hecht NB: MicroRNA Mirn122a reduces expression of the posttranscriptionally regulated germ cell transition protein 2 (Tnp2) messenger RNA (mRNA) by mRNA cleavage. Biol Reprod. 2005, 73 (3): 427-433. 10.1095/biolreprod.105.040998.View ArticlePubMedGoogle Scholar
- Hayashi K, Chuva de Sousa Lopes SM, Kaneda M, Tang F, Hajkova P, Lao K, O'Carroll D, Das PP, Tarakhovsky A, Miska EA, Surani MA: MicroRNA Biogenesis Is Required for Mouse Primordial Germ Cell Development and Spermatogenesis. PLoS ONE. 2008, 3 (3): e1738-10.1371/journal.pone.0001738.PubMed CentralView ArticlePubMedGoogle Scholar
- Griffiths-Jones S, Saini HK, van Dongen S, Enright AJ: miRBase: tools for microRNA genomics. Nucleic Acids Res. 2008, D154-158. 36 DatabaseGoogle Scholar
- Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A, Enright AJ: miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res. 2006, D140-144. 10.1093/nar/gkj112. 34 DatabaseGoogle Scholar
- Karolchik D, Kuhn RM, Baertsch R, Barber GP, Clawson H, Diekhans M, Giardine B, Harte RA, Hinrichs AS, Hsu F, Kober KM, Miller W, Pedersen JS, Pohl A, Raney BJ, Rhead B, Rosenbloom KR, Smith KE, Stanke M, Thakkapallayil A, Trumbower H, Wang T, Zweig AS, Haussler D, Kent WJ: The UCSC Genome Browser Database: 2008 update. Nucleic Acids Res. 2008, D773-779. 36 DatabaseGoogle Scholar
- Sanger Institute. [http://microrna.sanger.ac.uk/targets/v5/]
- TargetScanHuman. [http://www.targetscan.org/]
- DIANA-microTest. [http://www.diana.pcbi.upenn.edu/cgi-bin/micro_t.cgi]
- PicTar. [http://pictar.mdc-berlin.de/]
- MiRGen. [http://www.diana.pcbi.upenn.edu/miRGen.html]
- Obernosterer G, Martinez J, Alenius M: Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections. Nat Protoc. 2007, 2 (6): 1508-1514. 10.1038/nprot.2007.153.View ArticlePubMedGoogle Scholar
- Dillon N: Heterochromatin structure and function. Biol Cell. 2004, 96 (8): 631-637. 10.1016/j.biolcel.2004.06.003.View ArticlePubMedGoogle Scholar
- Bentwich I, Avniel A, Karov Y, Aharonov R, Gilad S, Barad O, Barzilai A, Einat P, Einav U, Meiri E, Sharon E, Spector Y, Bentwich Z: Identification of hundreds of conserved and nonconserved human microRNAs. Nat Genet. 2005, 37 (7): 766-770. 10.1038/ng1590.View ArticlePubMedGoogle Scholar
- Tanzer A, Stadler PF: Molecular evolution of a microRNA cluster. J Mol Biol. 2004, 339 (2): 327-335. 10.1016/j.jmb.2004.03.065.View ArticlePubMedGoogle Scholar
- Rockett JC, Patrizio P, Schmid JE, Hecht NB, Dix DJ: Gene expression patterns associated with infertility in humans and rodent models. Mutat Res. 2004, 549 (1–2): 225-240.View ArticlePubMedGoogle Scholar
- Braun RE: Post-transcriptional control of gene expression during spermatogenesis. Semin Cell Dev Biol. 1998, 9 (4): 483-489. 10.1006/scdb.1998.0226.View ArticlePubMedGoogle Scholar
- Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H: Clustering and conservation patterns of human microRNAs. Nucleic Acids Res. 2005, 33 (8): 2697-2706. 10.1093/nar/gki567.PubMed CentralView ArticlePubMedGoogle Scholar
- Voorhoeve PM, le Sage C, Schrier M, Gillis AJ, Stoop H, Nagel R, Liu YP, van Duijse J, Drost J, Griekspoor A, Zlotorynski E, Yabuta N, De Vita G, Nojima H, Looijenga LH, Agami R: A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell. 2006, 124 (6): 1169-1181. 10.1016/j.cell.2006.02.037.View ArticlePubMedGoogle Scholar
- Novotny GW, Sonne SB, Nielsen JE, Jonstrup SP, Hansen MA, Skakkebaek NE, Rajpert-De Meyts E, Kjems J, Leffers H: Translational repression of E2F1 mRNA in carcinoma in situ and normal testis correlates with expression of the miR-17-92 cluster. Cell Death Differ. 2007, 14 (4): 879-882. 10.1038/sj.cdd.4402090.View ArticlePubMedGoogle Scholar
- Lin WW, Lamb DJ, Lipshultz LI, Kim ED: Demonstration of testicular apoptosis in human male infertility states using a DNA laddering technique. Int Urol Nephrol. 1999, 31 (3): 361-370. 10.1023/A:1007130320700.View ArticlePubMedGoogle Scholar
- Zeng Y, Rosborough RC, Li Y, Gupta AR, Bennett J: Temporal and spatial regulation of gene expression mediated by the promoter for the human tissue inhibitor of metalloproteinases-3 (TIMP-3)-encoding gene. Dev Dyn. 1998, 211 (3): 228-237. 10.1002/(SICI)1097-0177(199803)211:3<228::AID-AJA4>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Schumacher V, Gueler B, Looijenga LH, Becker JU, Amann K, Engers R, Dotsch J, Stoop H, Schulz W, Royer-Pokora B: Characteristics of testicular dysgenesis syndrome and decreased expression of SRY and SOX9 in Frasier syndrome. Mol Reprod Dev. 2008, 75 (9): 1484-1494. 10.1002/mrd.20889.View ArticlePubMedGoogle Scholar
- Ying J, Srivastava G, Hsieh WS, Gao Z, Murray P, Liao SK, Ambinder R, Tao Q: The stress-responsive gene GADD45G is a functional tumor suppressor, with its response to environmental stresses frequently disrupted epigenetically in multiple tumors. Clin Cancer Res. 2005, 11 (18): 6442-6449. 10.1158/1078-0432.CCR-05-0267.View ArticlePubMedGoogle Scholar
- Martin RH: Meiotic chromosome abnormalities in human spermatogenesis. Reprod Toxicol. 2006, 22 (2): 142-147. 10.1016/j.reprotox.2006.03.013.View ArticlePubMedGoogle Scholar
- Lamb NE, Hassold TJ: Nondisjunction – a view from ringside. N Engl J Med. 2004, 351 (19): 1931-1934. 10.1056/NEJMp048118.View ArticlePubMedGoogle Scholar
- Sun F, Greene C, Turek PJ, Ko E, Rademaker A, Martin RH: Immunofluorescent synaptonemal complex analysis in azoospermic men. Cytogenet Genome Res. 2005, 111 (3–4): 366-370. 10.1159/000086913.View ArticlePubMedGoogle Scholar
- Marcon E, Babak T, Chua G, Hughes T, Moens PB: miRNA and piRNA localization in the male mammalian meiotic nucleus. Chromosome Res. 2008, 16 (2): 243-260. 10.1007/s10577-007-1190-6.View ArticlePubMedGoogle Scholar
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