In situ hybridization of miRNA in human testes. In situ hybridization analyses using 5' DIG-conjugated, LNA-modified DNA probe complementary to miR-383 was performed on 10-μm frozen sections of the testes of NOA patient and normal control. A, D: HE stain of normal (A) and NOA testes (E); B, E: In the testis of normal control (B), miR-383 was highly expressed in primary spermatocyte (PS) and lowly expressed in spermatid (Sp); whereas the expression of miR-383 in NOA patient (E) is decreased compared with control. C, F: The negative control of normal (C) and NOA testes (F), where the tissues were treated without using DIG-labelled probe. Scale bar = 50 μm.