Table 1 Oligonucleotides used in this study.
From: Altered microRNA expression in patients with non-obstructive azoospermia
Primer set name | Reverse transcriptase reaction primer (5' to 3') | Real-time quantitative PCR primer (5' to 3') |
|---|---|---|
U6 | CGCTTCACGAATTTGCGTGTCAT | Forward: GCTTCGGCAGCACATATACTAAAAT Reverse: CGCTTCACGAATTTGCGTGTCAT |
hsa-miR-491-3p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGTAGAA | Forward: TGCTTATGCAAGATTCCC Reverse: TGCGTGTCGTGGAGTC |
hsa-miR-302a | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCACCAA | Forward: GGGGTAAGTGCTTCCATGTT Reverse: CAGTGCGTGTCGTGGAGT |
hsa-miR-520d-3p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACCCAC | Forward: GGGGAAAGTGCTTCTCTTTG Reverse: GTGCGTGTCGTGGAGTCG |
hsa-miR-383 | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGCCAC | Forward: GGGAGATCAGAAGGTGATT Reverse: TGCGTGTCGTGGAGTC3 |