Role of cervical dendritic cell subsets, co-stimulatory molecules, cytokine secretion profile and beta-estradiol in development of sequalae to Chlamydia trachomatis infection
© Agrawal et al; licensee BioMed Central Ltd. 2008
Received: 16 June 2008
Accepted: 01 October 2008
Published: 01 October 2008
Chlamydia trachomatis infection of the female genital tract can lead to serious sequelae resulting in fertility related disorders. Little is known about the mechanism leading to Chlamydia induced pathology and factors responsible for it. As only some of the women develops reproductive disorders while majority of the women clears infection without any severe sequalae, mucosal immune response in women with or without fertility disorders was studied to identify factors which may lead to final clinical outcome of chlamydial infection.
Myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) populations in cervical mucosa and peripheral blood were analyzed in controls and Chlamydia positive women with or without fertility disorders with multicoloured flow cytometric analysis. Cervical cytokines (IL-6, IL-8, IL-10, IL-12, TNF-alpha and IFN-gamma), C-reactive protein levels and sex hormone levels in serum were quantified by ELISA.
In cervix of Chlamydia positive women with fertility disorders, significantly high (P < 0.05) numbers of pDCs were present with increased CD80 expression. pDCs correlated significantly with C-reactive protein levels, IL-6 and IFN-gamma levels in women with fertility disorders. In contrast, mDCs showed significant upregulation of CD1a during chlamydial infection and correlated significantly with IL-12 levels in Chlamydia positive fertile women. β-estradiol levels were significantly higher in women having fertility disorders as compared to fertile women and have significant correlations (r = 0.65; P < 0.05) with pDCs numbers, CD80 expression, IL-6 levels and IFN-gamma levels in these women.
These results suggest that development of sequalae in some women can be a result of interplay of many factors including type of dendritic cell, co stimulatory molecule expression, cytokine secretion pattern and hormone levels.
Chlamydia trachomatis infections are the most prevalent sexually transmitted bacterial infections worldwide . In India, a high prevalence rate of genital chlamydial infection has been reported among symptomatic women, of which upto 30% have fertility related disorders as multiple spontaneous abortions, infertility (not male related), or sub fertility [2, 3]. Spontaneous clearance of C. trachomatis from the lower genital tract occurs in nearly 20% of chlamydial infections without any sequelae , however, in absence of treatment, these infections often recur, or remain persistent, leading to structural damage to the inflamed tissue and increase the risk of developing a sequelae [5, 6]. Host factors such as type of immune response, plays a large role in determining the course and morbidity of C. trachomatis infections but the exact immunopathological mechanism leading to Chlamydia induced sequelae is still not well understood.
During chlamydial infection, T-cell mediated adaptive immune responses, play a major role in the clearance and resolution of infection . T cells are activated by antigen presenting cells as dendritic cells (DCs) which play a crucial role in initiation and maintenance of T-cell immunity . Besides providing protection, DCs have also been reported to be involved in chronic inflammation . The cervical mucosa is reported to contain numerous DCs interdigitating between the epithelial cells  and these DCs often stain positive for CD1a . DCs have also been reported to produce large amounts of interleukin-12 (IL-12) upon ex vivo pulsing with inactivated chlamydial organisms .
Two major DC subsets have been described; the CD123+ DCs designated as plasmacytoid DCs (pDCs) and the CD11c+CD123- (-/dim) designated as myeloid DCs (mDCs). the subsets express high levels of HLA-DR and lack the lineage markers CD3, CD14, CD19, CD20, CD16, and CD56, however, functional differences between the two have been described which include T-cell stimulatory activity, production of proinflammatory cytokines and differential expression of co-stimulatory molecules [13–15]. The expression of various co-stimulatory molecules (CD80/86) associated with DCs, have also been reported to modulate the type of immune response . Further upon chlamydial infection, mononuclear cells are triggered to release a number of proinflammatory cytokines including TNF-α, IL-1, IL-6 and IL-8 [17, 18] and many studies have reported association between cytokine profiles and immunopathogenic mechanisms. We have also reported previously that estradiol levels can modulate immune response in women with chlamydial infection  and can have a role in Chlamydia induced pathology.
In a recent study from our laboratory  we have reported that chlamydial infection recruits both mDCs and pDCs to the cervix and suggested that pDCs may be responsible for the immunopathogenesis of chlamydial infection, thereby, helping in the induction of sequelae. These results along with above facts prompted us to study the mobilization of these DC subsets in Chlamydia positive women, with or without fertility related disorders (multiple spontaneous abortions and infertility (not male related) and to evaluate the role played by these DC subsets in providing a protective/pathogenic immune response. We also measured the expression of various co-stimulatory molecules associated with these DCs and cytokines in cervical washes. C-reactive protein (CRP) levels and sex hormone levels in the sera of women with and without fertility disorders were also studied to understand the basic question in chlamydial pathogenesis as to why some women clear infection while others develop sequelae.
After obtaining informed written consent, 153 patients attending the gynecology outpatient department, Safdurjang Hospital, New Delhi, India were enrolled for the study. Twenty eight healthy age-matched controls attending the family planning department for birth control measures and with no previous history of any sexually transmitted disease (STD) were also enrolled. The study received approval from the hospital's ethics review committee. Procedures followed for sample collection were in accordance with the ethical standards for human experimentation established by the Declaration of Helsinki of 1975 (revised in 1983).
At recruitment, a detailed clinical questionnaire was administered to each patient for collecting information on reasons for referral, gynecology history including menstruation, symptoms of genital and urinary tract infection, obstetric and medical histories. Patients taking oral contraceptives, having positive urine pregnancy test, recent antibiotic therapy, and history of recently treated sexually transmitted infection and genital tuberculosis were excluded from the study.
Women with fertility related disorders included women with infertility and multiple spontaneous abortions. Infertile women where identified as those, which lack recognized conception after 1.5 to 2 years of regular intercourse without the use of contraception. Women with multiple spontaneous abortions (more than 2) have been described as those having delivery of pre-viable foetus before the 20th week of gestation. Fertile women were those having last child birth within last 4 months to 1 year and testing positive for C. trachomatis during last pregnancy.
Since variations in sex hormones are known to influence cytokines concentrations and immune cell populations including the mDC and pDC , cervical samples were collected during mid-cycle (median 13 days, range 9th to 15th day of the menstrual cycle). None of the patient had sexual intercourse 3 days or more prior to collection of sample.
Collection of samples
After cleaning the endocervix, endocervical swabs (HiMedia, Mumbai, India) were collected for diagnosis of C. trachomatis and other sexually transmitted disease (STD) pathogens. Cervical washes for determination of cervical cytokines were collected in 5 ml of sterile saline administered through a sterile Pasteur pipette and recovered after thorough washing of the cervix. For collection of cervical cells, a cytobrush was placed within the endocervical canal so that the cells from the endocervical region and the zone between the endocervical and ectocervical region (transformation zone) could be obtained. Cells were then transferred to a sterile tube containing sterile PBS (pH 7.2) supplemented with 100 U penicillin/mL, 100 μg streptomycin/mL, and 100 μg glutamine/mL. No samples were collected from patients with friable cervix and contact bleeding to ensure collection of cervical lymphocytes only. Heparinized peripheral venous blood (5 mL), was also collected after the diagnostic samples. Samples were then stored at 4°C until, they were transported to the laboratory and were then processed within 1 h.
Samples were confirmed for chlamydial positivity by Direct Fluorescent Analysis (DFA) using fluorescein isothiocyanate (FITC) conjugated monoclonal antibodies to C. trachomatis major outer membrane protein (Microtrak, Palo Alto, USA). A sample was considered to be positive when greater than 10 elementary bodies (EB's) were detected. DFA negative samples were further confirmed for positivity by PCR analysis using primer specific for 517 bp plasmid of C. trachomatis . Gram stained cervical smears were examined for the presence of yeast cells (Candidiasis) and quantification of polymorphonuclear leukocytes (PMNLs) per high-powered field (hpf) was done in smears to ensure presence of cervicitis. Vaginal smears were analyzed for clue cells, for diagnosis of bacterial Vaginosis. Wet mount microscopy was performed for the diagnosis of Trichomonas vaginalis. Neisseria gonorrhoeae, Mycobacterium hominis and Ureaplasma urealyticum were detected by culture as described previously .
Isolation of cells from cervical samples
Cervical cells were isolated from the cytobrush by vigorously rotating it against the sides of the transport tube after incubating the sample with 5 mM DL-dithiothreitol (Sigma, St Louis, MO) at 37°C for 15 min (to reduce the mucus component of the sample). The cell suspension obtained was then filtered through a sterile 70 μm nylon cell strainer (BD Biosciences, San Diego, USA) and centrifuged at 300 g for 10 min; the resultant pellet yielding endocervical cells. Viability of cells was determined by trypan blue exclusion assay. The population of epithelial cells and lymphocytes in the cytobrush sample was counted with a haemocytometer and samples containing less than 1 million lymphocytes/ml were excluded.
mDCs and pDCs in cervical secretions were measured as number of events present per 100,000 cells taken for experiment. This number was then adjusted according to the total number of cells obtained in that cervical sample. These were finally presented as number of events per cervical sample. In case of blood the dendritic cell subsets were counted as events per 100 μl of blood taken and were then calculated and represented as events per milliliter of blood.
Quantification of cytokines in cervical washes
Quantification of IL-4, IL-6, IL-8, IL-10, IL-12, TNF-α and IFN-γ was done using commercially available ELISA kits (eBiosciences, San Diego, CA), in accordance with the manufacturer's instructions. Briefly, 96-well ELISA plates were coated overnight with antihuman IL-4, IL-6, IL-10, IL-8, IL-12, TNF-α and IFN-γ capture antibodies. Unbound coating antibody was removed, and nonspecific protein binding sites were blocked with assay diluent as per the manufacturer's instructions. Duplicate serial dilutions of test samples or controls were incubated for 2 h at ambient temperature. Detector antibodies were added and incubated for 1 h, followed by incubation with peroxidase-conjugated antimouse IgG for 30 min. For color development, tetramethylene benzidine was added and then stopped after 15 min by the addition of 1 N sulfuric acid. Absorbance was read at 450 nm with reference absorbance of 650 nm. A log log standard curve was generated, and unknowns were interpolated. The sensitivities of cytokine kits were 1 pg/mL.
Determination of C-reactive protein levels
C-reactive protein levels in sera were measured by commercially available hs-CRP ELISA kit (Calbiotech, USA) and levels above 2 microgram per millilter were considered to show higher risk of chronic inflammation as per the manufacturer's instructions.
Levels of β-Estradiol and progesterone in sera of patients and controls were measured using commercially available ELISA kits (DRG, International Inc, USA) as per the manufacturer's instructions.
The Kruskal-Wallis non parametric test was used to compare continuous variables among multiple groups. Categorical variables were compared using χ2 test. Correlation was determined with spearman's correlation coefficient. P < 0.05 was considered to be significant.
Cervical C. trachomatis infection was diagnosed by Direct Fluorescence Assay/PCR in 65 patients. Nine of these patients were co-infected either with Candida spp., bacterial vaginosis, T. vaginalis, M. hominis, U. urealyticum, or with N. gonorrhoeae and were thus excluded from the study. Two Chlamydia positive patients were excluded due to lower mononuclear cell count (less than 1 million cell/ml). Chlamydia positive women were divided into two groups: (a) Chlamydia positive fertile women (n = 34) and (b) Chlamydia positive women with fertility related disorders (n = 20). No significant differences in the median ages of the patients (fertile and with fertility disorders) or controls was observed (27, 27 and 28 years respectively).
Immune cell population in the cervix
Flowcytometric analysis demonstrated the presence of mDCs, pDCs, CD14+ monocytes, CD3+CD4+ T cells and CD3+CD8+ T cells in both the Chlamydia positive groups and controls. The median range of CD3+CD4+ T cells and CD3+CD8+ T lymphocytes among endocervical leucocytes in women which were included in the study was between 59% and 86% that of B lymphocytes was between 1% and 4%. Significant increase was seen in the mean number of CD4+ T-lymphocytes per 10,000 events in cervical mucosa of fertile women compared to women with fertility disorders and controls (2195 versus 401 and 262 respectively; P < 0.05). In contrast, the CD8+ T cell population in cervical mucosa was found to be high during chlamydial infection but was non significant compared to controls. The number of CD14+ monocytes per 10 000 events was found to be significantly increased in women with chlamydial infections compared to controls (324 versus 312 and 147; P < 0.05 for fertile women, women with fertility disorders and controls respectively).
mDC and pDC population in cervical mucosa and peripheral blood
Absolute median numbers of LIN-DR+ CD11c+ (mDCs) and LIN-DR+CD123+ (pDCs) dendritic cells in cervix (per cervical sample) and peripheral blood (per milliliter) of Chlamydia trachomatis positive and negative women.
CT Positive Fertile
(n = 34)
CT Positive with Fertility Disorders
(n = 20)
(n = 28)
Expression of cell surface markers on mDC and pDC in cervical mucosa
The percentage expression of various co-stimulatory molecules on the surface of mDCs and pDCs subsets in cervical mucosa.
% expression on mDCs
% expression on pDCs
(n = 34)
CT+ with FD
(n = 20)
(n = 28)
(n = 34)
CT+ with FD
(n = 20)
(n = 28)
16.0 ± 3.0a
12.0 ± 2.2
7.0 ± 0.06
8.0 ± 0.7
32.5 ± 2.5b
6.0 ± 0.5
23.0 ± 0.6a
28.0 ± 1.8a
11.0 ± 2.0
18.0 ± 2.0a
38.0 ± 2.4b
8.0 ± 0.7
62.0 ± 7.0
59.0 ± 2.6
82.0 ± 5.0
21.0 ± 4.0
20.0 ± 1.9
31.0 ± 2.0
43.0 ± 3.0a
37.0 ± 3.8a
18.0 ± 3.0
45.0 ± 3.0
41.0 ± 2.7
28.0 ± 1.0
Concentration of cytokines in cervical washes and their correlation with DC populations
Cytokine concentrations in cervical washes of Chlamydia positive women and controls.
CT positive fertile women
CT positive women with fertility Disorder
Significant correlation (r = 0.65; P <0.05) was observed between number of mDCs/cervical sample and levels of IL-12 in Chlamydia positive fertile women. Number of pDCs/cervical sample in women with fertility disorders showed significantly high correlation with IL-6 levels (r = 0.78; P < 0.01) and IFN-γ levels (r = 0.58; P < 0.05). A less apparent correlation of IL-8 levels with pDCs/cervical sample in women with fertility disorders was observed (r = 0.41). CD80 expression on cervical mDCs from Chlamydia positive fertile women showed correlation with IL-12 levels (r = 0.56; P < 0.05). CD80 expression on pDCs from Chlamydia positive women with fertility disorders showed significant correlation with both IL-12 and IFN-γ levels (r = 0.73; P < 0.01 and r = 0.88; P = 0.001 respectively). CD86 levels showed significant correlation with IL-10 levels (r = 0.62; P < 0.05).
Correlation of mDC and pDCs/cervical sample with C-reactive protein levels and sex hormone levels
Levels of β-estradiol, CRP and Progesterone in serum of controls and Chlamydia positive women.
In women, chlamydial infections are often asymptomatic, and subsequent reinfections lead to inflammatory responses with pathological sequelae . In peripheral circulation, majority of LIN-/DR+ DCs cells express either CD11c or CD123 molecules [25, 26], but no information is available on presence and role of these subsets during chlamydial infection. In this study, median number of pDCs in the cervix was found to be significantly higher than mDCs in both the Chlamydia positive groups. The number of cervical mDCs was significantly higher in fertile women in contrast to number of pDCs which were much higher in women with fertility disorders. These results depict that different pathological conditions are associated with different phenotype of dendritic cells and in women having sequelae to chlamydial infection, pDCs outnumber mDCs in the cervix. A previous study has also demonstrated enhanced number of pDC count in bronchoalveolar lavage fluid of immunocompetent patients with pneumonia . Another study by Hartmann et al., have also shown high number of pDCs in patients with upper respiratory tract infection . In comparison to mDCs, high number of pDCs were reported to be present in synovial fluid from patients with spondyloarthropathy . We have also reported  higher number of mDCs in Chlamydia positive women with non-inflamed cervix compared to pDCs which were abundant in inflamed cervix. Beulens et al., have originally described mDCs as functionally mature DCs with a strong T cell stimulatory capacity  and have been reported to induce protective immune response , whereas, pDCs are generally defined as tolerogenic dendritic cells. Thus, these results collectively suggests that mDCs provide protective response while pDCs are involved in pathogenesis but how they help in development of a disorder is not well understood.
As for immune modulation by costimulatory molecules, previous studies have demonstrated that CD80/CD86 co-stimulatory molecules differentially activate TH1/TH2 type pathway and act as co-stimulatory signals for generation of TH1/TH2 cells CD4+ T cells [30, 31]. It has been shown previously that silencing of CD80 expression on dendritic cells significantly decreases IFN-γ secretion while that of CD86 decreases IL-4 secretion . CD86 has also been shown to stimulate IL-10 production in CD4+ T cells . Significantly high expression of CD80 molecules on pDCs obtained from women with fertility disorders and on mDCs from fertile women was observed. This suggests that significantly high expression of CD80 may lead to increased activation of TH1 cells during Chlamydial infection. As no difference in expression levels of CD86 among different groups was observed it suggests that CD86 may not be involved in induction of immune response to C. trachomatis.
Further, our results showed significantly higher expression of CD83 on both cervical mDCs and pDCs compared to controls. In comparison to mDCs the expression was significantly high on pDCs from women with fertility disorders. CD83 expression is taken as a marker for phenotypic maturation of dendritic cells and higher percentage of DCs expressing them in both Chlamydia positive cases show that during chlamydial infection high number of both mDC and pDC take up antigen for processing and turn into mature DCs. This data suggests that although both mDCs and pDCs are present in the cervix of women with fertility disorders but still pDCs are the ones which are more matured and hence activated.
When cervical washes were studied for cytokine concentrations it was seen that in Chlamydia positive women with fertility disorders significantly higher levels of IL-6, IL-8, IL-10 and IFN-γ were present compared to the other groups. In comparison to this IL-12 levels were significantly higher in Chlamydia positive fertile women. Although not as protective as IFN-γ, IL-6 (generated either by epithelial cells or by the interaction of chlamydiae with T lymphocytes) is probably important, together with IL-12, for sustaining the protective T-helper 1 cell mediated immune response . Although protective in nature, pathogenic role of IL-6, has been shown previously [35, 36]. In earlier study increased IL-6 levels during in silent tubal infections of C. trachomatis have been reported . As for IL-8, it has also been shown previously that synovial tissues from chronic arthritis patients with synovial C. pneumoniae infection have significant levels of mRNA for IL-8 . These results suggest that IL-6 and IL-8 may be involved in chlamydial pathology but their actual role and mechanism is yet to be ascertained.
IL-10 was found to be up-regulated in cervical washes obtained from Chlamydia positive women with fertility disorders. IL-10 is not always an inflammatory/inhibitory cytokine; instead higher levels of IL-10 probably prevent the pathological effect of the inflammatory cytokines like IL-1β, IFN-γ and TNF-α. IL-10 can create a favourable environment for persistence of microbes by down-regulating the proinflammatory cytokines. In case of chlamydial infection IL-10 has been reported to be associated with typical pathological changes like fibrosis and granuloma formation . In case of ocular chlamydial infection, IL-10 has been shown to be associated with scarring and blindness . Association of increased frequency of IL-10 detection in endocervical secretions of women with non-ulcerative STDs, like C. trachomatis have suggested that this may be a potential mechanism through which these infections may alter susceptibility to HIV-1 infection . These results along with ours, suggests, that excessive secretion of IL-10 may lead to either incomplete clearance of bacteria or may develop a state of anergy resulting in development of fertility related disorders.
IL-12, on the other hand was significantly higher in Chlamydia positive fertile women compared to other groups. IL-12 is derived from dendritic cells and monocytes and it induces TH1 differentiation with induction of IFN-γ . These results suggest that high secretion of IL-12 may be responsible for providing a protective immune response to chlamydial infection.
IFN-γ levels were found to be significantly higher in women with fertility disorders as compared to other groups. A previous study by our group  have shown also high levels of IFN-γ in cervical washes of women with recurrent chlamydial infections compared to women with primary chlamydial infections and has demonstrated that high secretion of pro-inflammatory cytokines as IL-6, IFN-γ and TNF-α lead to acute inflammation and can thus cause reproductive disorders. An earlier study by Van Voorhis et al., 1997 has also shown that repeated chlamydial infection in a Macca nemestrina model high levels of IFN-γ transcripts were produced . Further, in synovial tissue of patients with C. trachomatis associated arthritis, both IL-10 and IFN-γ producing cells were detected and it was suggested that excessive IL-10 production suppresses IFN-γ and mediated persistence .
High expression of CD80 on mDCs and its significant correlation with IL-12 levels in fertile women further suggest that the major role of mDCs is in clearance of the organism from the genital tract. On the other hand, CD80 expression on pDCs of women with fertility disorders showed significant correlation with both IL-12 and IFN-γ levels suggesting the role of CD80 activation in secretion of type 1 cytokines. Further, these results suggest that modulation of cytokine expression can be the main mechanism which decides whether the infection is cleared or will go for pathological damage. This would however, be hypothetical as there is no confirmation that in women with fertility related disorders the clinical condition is due to chlamydial infection and not due to any other cause. The results we got above may shed a new light on chlamydial pathogenesis but these have their limitations as; (1) scarcity of cell numbers making it impossible for us to separate these subsets and then see the expression of cytokines upon stimulation with C. trachomatis as increased number of pDCs can act as a factor acting to limit bacterial load and pathogenesis (2) the fact that cytokines like IL-6, IL-8 and IL-12 are secreted by other cell populations like macrophages and epithelial cells which outnumber DCs in the cervix.
Further, median CRP levels were found to be significantly higher in women with fertility disorders and showed significant correlation with pDCs. CRP levels are considered marker for inflammation and its association with pDCs in women with fertility disorders suggests that pDCs are involved either in induction or in maintenance of inflammatory responses.
We detected significantly high levels of estradiol in women with fertility disorders, which correlated significantly with pDC levels, CD80 expression and IL-6 and IFN-γ secretion. In a previous study in humans it has been shown that women are more susceptible to chlamydial infection under β-estradiol influence, since more chlamydial organisms can be isolated during the proliferative part of the cycle . Estrogen was also found to enhance chlamydial adherence or intracellular development of their inclusion . This suggests that estradiol may enhance inflammation by helping in increased secretion of proinflammatory cytokines either by up-regulating CD80 expression or by modulating the cytokine secretion profile of other cell. A previous study by Dimayuga et al.,  has also shown up-regulation of CD80 expression in LPS stimulated microglial cells by estrogen treatment. Overall these results show that high estradiol levels may help in persistence of Chlamydia directly by helping in its intracellular development or indirectly by modulation of cytokine secretion profile of immune cells.
Overall, our data suggests that a differential activation of subsets of DC, cytokine secretion pattern and hormonal levels can be responsible for modulating the immune response to chlamydial infection, but, the question that still remains unanswered is the mechanism by which these are modulated during chlamydial infections and this deserves further elucidation. Since there is an urgent need to develop a vaccine against chlamydial infection, therefore, the basic research challenge is to understand the requirements for inducing and maintaining protective genital mucosal immunity.
We thank Mrs. Madhu Badhwar, Mrs. Asha Rani and Mrs. Rosamma Thomas for providing technical assistance. This study was supported by an Indo-US grant (BT/IN/USCRHR/AM/2002) from Department of Biotechnology, Government of India. University Grants Commission is acknowledged for providing financial assistance to Tanvi Agrawal as fellowship.
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