- Open Access
Involvement of Src family of kinases and cAMP phosphodiesterase in the luteinizing hormone/chorionic gonadotropin receptor-mediated signaling in the corpus luteum of monkey
© Kunal et al; licensee BioMed Central Ltd. 2012
- Received: 10 November 2011
- Accepted: 29 March 2012
- Published: 29 March 2012
In higher primates, during non-pregnant cycles, it is indisputable that circulating LH is essential for maintenance of corpus luteum (CL) function. On the other hand, during pregnancy, CL function gets rescued by the LH analogue, chorionic gonadotropin (CG). The molecular mechanisms involved in the control of luteal function during spontaneous luteolysis and rescue processes are not completely understood. Emerging evidence suggests that LH/CGR activation triggers proliferation and transformation of target cells by various signaling molecules as evident from studies demonstrating participation of Src family of tyrosine kinases (SFKs) and MAP kinases in hCG-mediated actions in Leydig cells. Since circulating LH concentration does not vary during luteal regression, it was hypothesized that decreased responsiveness of luteal cells to LH might occur due to changes in LH/CGR expression dynamics, modulation of SFKs or interference with steroid biosynthesis.
Since, maintenance of structure and function of CL is dependent on the presence of functional LH/CGR its expression dynamics as well as mRNA and protein expressions of SFKs were determined throughout the luteal phase. Employing well characterized luteolysis and CL rescue animal models, activities of SFKs, cAMP phosphodiesterase (cAMP-PDE) and expression of SR-B1 (a membrane receptor associated with trafficking of cholesterol ester) were examined. Also, studies were carried out to investigate the mechanisms responsible for decline in progesterone biosynthesis in CL during the latter part of the non-pregnant cycle.
Results and discussion
The decreased responsiveness of CL to LH during late luteal phase could not be accounted for by changes in LH/CGR mRNA levels, its transcript variants or protein. Results obtained employing model systems depicting different functional states of CL revealed increased activity of SFKs [pSrc (Y-416)] and PDE as well as decreased expression of SR-B1correlating with initiation of spontaneous luteolysis. However, CG, by virtue of its heroic efforts, perhaps by inhibition of SFKs and PDE activation, prevents CL from undergoing regression during pregnancy.
The results indicated participation of activated Src and increased activity of cAMP-PDE in the control of luteal function in vivo. That the exogenous hCG treatment caused decreased activation of Src and cAMP-PDE activity with increased circulating progesterone might explain the transient CL rescue that occurs during early pregnancy.
- cAMP-Phosphodiesterase (PDE)
- Corpus luteum
- Src family of kinases (SFKs)
The primary function of corpus luteum (CL) is to secrete progesterone (P4), essential for establishment and/or maintenance of pregnancy in mammals [1, 2]. The structure and function of CL are controlled by luteotrophic factors (stimulate growth and function) and luteolytic factors (cause functional and structural regression). It is increasingly becoming apparent that there exists a large diversity in the regulation of CL function not only among species, but also within species at different stages of the luteal phase dictated largely by the intricate interplay between luteotrophic and luteolytic factors. In higher primates, one of the important characteristics of the regulation of CL function is that unlike other species, circulating LH appears to be the sole trophic factor responsible for its control during the non-fertile cycle . Intriguingly, circulating P4 levels decline and the CL eventually undergoes regression at the end of non-fertile cycle despite the accompanying lack of decrease in LH levels [4, 5]. On the other hand, during pregnancy the increasing production of chorionic gonadotropin (CG) elaborated by the placenta arrests the decline in P4 secretion resulting in rescue of CL function . Although recent studies continue to expand our current understanding of the cellular and molecular actions of LH/CG, knowledge of the processes whereby LH/CG promote development and maintenance of CL function is far from clear. Though LH and CG bind to a common LH/CG receptor (R), they activate different signal transduction pathways for maintenance of CL function as reported in spheroidal cell culture system of human granulosa lutein cells [7, 8]. The LH/CGR is a G protein-coupled receptor (GPCR) involving activation of Gs, Gi/o and Gq/11 family of G proteins [9–12], but it is generally accepted that modulation of Leydig/luteal cell function is mediated primarily by the activation of the canonical Gs/cAMP/PKA/CREB pathway [13, 14]. Analysis of cAMP/PKA/CREB and MAP kinase pathways in the luteal tissue has suggested fine tuning of luteal function during luteolysis and rescue of CL function, however, involvement of these pathways alone does not appear to fully account for the regulation of CL function . Recently, several studies have described the phenomenon of activated GPCRs to crosstalk with or transactivate tyrosine kinase receptor signaling [16, 17], and extensive studies in mouse Leydig tumor cells have demonstrated involvement of Src family of kinases (SFKs) in modulation of LH/CG responsiveness, and thereby regulation of Leydig cell function [18–21]. Eventhough the precise mechanism by which LH/CGR signaling causes activation of these kinases and the functional consequences of their activation remains to be determined, SFKs might have an important role in the regulation of CL function, perhaps by modulation of LH/CG responsiveness, particularly during the late luteal phase of non-fertile cycles. Thus, studies are essential to identify other signaling pathways to further delineate intraluteal processes to discern those that are critical to the control of luteal function and its life span.
To maintain high circulating P4 levels, steroid biosynthesis in luteal cells requires a constant supply of cholesterol . The intracellular cholesterol trafficking from outer to inner mitochondrial membrane, regulated by StAR protein, has been extensively investigated [23, 24]. On the other hand, the importance of cholesterol acquisition into luteal cell needs to be explored to further understand the process of substrate mobilization to the CL. It has been suggested that cholesterol is preferentially sourced from circulating high- and low- density lipoproteins (HDL and LDL) [25, 26]. Unlike the classical LDL receptor pathway in which the entire lipoprotein is internalized, the trafficking of cholesterol from HDL involves binding of HDL-cholesteryl ester to scavenger receptor class B type 1 (SR-B1) located on the luteal cell, and only cholesteryl ester is delivered without the concomitant uptake and degradation of the entire HDL particle, leaving the lipoprotein at the cell surface [27–31]. The expression changes of SR-B1 and its transcriptional regulation by factors such as Liver receptor homologue 1 (LRH-1/NR5A2) and Steroidogenic factor 1 (SF-1/NR5A1) become crucial to understanding the regulation of CL function [32, 33].
To further expand the knowledge on the regulation of CL function during spontaneous luteolysis in monkeys, studies have been carried out with following objectives: to (i) establish correlation between circulating P4 and the LH/CGR expression dynamics throughout the luteal phase (ii) determine the expression and activation of Src family of non-receptor tyrosine kinases and cAMP-phosphodiesterase (PDE), and (iii) to examine expression patterns of SR-B1 and genes associated with steroidogenesis in the CL.
Trizol reagent and GenElute™ Gel Extraction Kit were purchased from Sigma-Aldrich Co., (St Louis, MO, USA). Oligonucleotide primers were synthesized by Sigma-Genosys (Bangalore, India). Gonadotropin releasing hormone receptor (GnRH/R) antagonist, Cetrorelix® (CET) was a kind gift from Asta Medica (Frankfurt, Germany). Recombinant human LH (rhLH) and hCG (Profasi®) were from Ares-Serono (Aubonne, Switzerland). Labelled α-32P dCTP (LCP103) was from BRIT (Hyd., India) and Random Primer extension Labeling Kit (#KT04) was from Bangalore Genei (Bangalore, India). Polyclonal antibodies specific to LH/CGR (H-50: sc-25828), PDE4D (H-69: sc-25814) and ERK-2 (# sc-154) were from Santa Cruz Biotechnology Inc. (Santa Cruz city, CA, USA). Phospho-Src family (Y-416; #2101), Src Antibody sampler kit (#9935), p42/44 MAPK (# 9102), Horse radish peroxidase labeled goat anti-rabbit IgG and ECL chemiluminescence kit were from Cell Signaling Technology (Beverly, MA, USA). Polyclonal antibody against SR-B1 was a kind gift from Professor Salman Azhar (Stanford University School of Medicine). Snake venom nucleotidase (Crotalus atrox venom) was procured from Enzo Life Sciences, UK. Nylon and PVDF membranes were purchased from NEN life Sciences, (Boston, MA, USA). All other reagents were from Sigma-Aldrich Co., (Bangalore, India) or sourced from local distributors.
Animal protocols, blood samples, and CL collection
Experimental protocols in the monkeys described here were approved by the Institutional Animal Ethics Committee of the Indian Institute of Science. The general care and housing of monkeys at the Primate Research Laboratory, Indian Institute of Science, Bangalore, have been described elsewhere . Adult female bonnet monkeys (Macaca radiata) weighing 3.3 to 5.1 kg with a history of regular menstrual cyclicity (27-29 days) were utilized for the study. Monkeys were monitored daily for the onset of menses, and blood samples through femoral venipuncture were collected daily from day 8-12 of the menstrual cycle for determining the day of onset of E2 surge. Further, blood samples were collected either daily or at more frequent intervals until the time of CL retrieval or onset of menses. In this study, one day after the occurrence of E2 peak was designated as day 1 of the luteal phase. The CL tissues on the designated day of the luteal phase or at the end of treatment protocol (see below) were retrieved from the ketamine hydrochloride 15 mg/kg BW and/or pentobarbital sodium (8 mg/kg BW) anesthetized monkeys subjected to laparotomy under aseptic conditions. The CL retrieved was transferred to a sterile petri dish containing filter paper, cut into four or more pieces, a single piece each processed for cAMP and PDE assays. The remaining pieces were placed in individual sterile cryovials and snap frozen in liquid nitrogen before storing at -70°C.
Experiment 1: Expression of LH/CGR, genes belonging to SFKs, cAMP-PDE and SR-B1 in the monkey CL throughout the luteal phase
CL tissues (n = 3/stage) were collected from monkeys on day 5 [early (E)], day 8 [mid (M)], and on day 14 [late (L)] of the luteal phase as described previously [15, 34]. In addition, CL tissues (n = 3) were collected from monkeys on day 1 of menses (D1M), a time point when luteolytic events are considered to be manifest. Blood samples were collected on designated days, to determine the steroidogenic status of CL. The tissues retrieved during different developmental stages were used to examine the expression of genes considered important for the modulation of the molecular events that regulate CL structure and function during the luteal phase.
Experiment 2: Effect of PGF2α treatment on expression of LH/CGR, genes belonging to SFKs and SR-B1 in the monkey CL
PGF2α treatment induces luteolysis in various species without affecting the circulating LH levels . An experimental regimen has been standardized in the laboratory in which three injections of Iliren, a synthetic analogue of PGF2α (59 μg/kgBW) were administered at 8 h intervals on day 10 of the luteal phase and CL tissues (n = 3) were collected 24 h after initiation of treatment . Similarly, on a separate occasion, the same monkeys received three injections of PBS at 8 h intervals beginning on day 10 of the luteal phase and CL tissues were collected 24 h later to serve as control (VEH). Blood samples were collected at different intervals before and after treatment. The effect of PGF2α on expression of various genes was examined.
Experiment 3: Expression and regulation of LH/CGR, genes belonging to SFKs and SR-B1 in the monkey CL during induced luteolysis (LH secretion inhibition) and rescue of CL function (LH replacement)
To induce luteolysis, monkeys were administered with GnRH-R antagonist, CET at a dose of 150 μg/kgBW s.c., on day 7 of the luteal phase, similarly on separate occasions, the same monkeys received vehicle (5.25% glucose; VEH) and CL tissues were collected 24 h after CET/VEH treatment. Further, on day 8 of the luteal phase, six monkeys treated with CET for 24 h were further treated with either PBS (VEH, n = 3 monkeys) or rhLH 20 IU/kgBW (rhLH, n = 3) i.v., and CL tissues were collected at the end of 1 and 8 h rhLH treatment . Blood samples were collected at various time points before and after treatment/s essentially as per the earlier reported protocol . The tissues obtained after LH secretion inhibition and rescue of CL function by LH replacement were subjected to examination of various genes, SFKs activation and cAMP levels.
Experiment 4: Expression and regulation of LH/CGR, genes belonging to SFKs and SR-B1 in CL during simulated early pregnancy
It is established that events associated with rescue of CL function that occur during early pregnancy could be simulated in non-fertile monkeys by administration of incremental doses of hCG . To examine the expression of LH/CGR, genes belonging to SFKs and SR-B1 during simulated early pregnancy, monkeys were administered hCG in incremental doses from day 9-13 of the luteal phase (15, 30, 45, 90, and 180 IU, twice daily, i.m.) and CL tissues were retrieved on d 14 of the luteal phase as per the protocol previously reported . For comparison, CL collected on day 14 (late CL-L) of the luteal phase but without hCG treatment, was used as the control.
Serum P4 concentrations were determined by specific RIAs as reported previously . The sensitivity of P4 assay was 0.1 ng/ml, and the inter- and intra-assay variations were < 10%.
Preparation of CL tissue samples for cAMP assay
Fresh CL tissues collected from different experiments were boiled immediately in boiling water (95°C) bath for 10 min to inactivate the PDE enzyme and then homogenized in sterile H2O and centrifuged at 30,000 × g for 15 min. The supernatants were assayed for cAMP and to increase the sensitivity of the assay, samples were acetylated i.e., 100 μl of supernatant was made up to 1.5 ml final volume using 1X PBS, 30 μl of triethylamine and 15 μl of acetic anhydride and mixed properly for acetylation process to complete. These acetylated samples were used for cAMP RIA.
Radioimmunoassay for cAMP
The assay procedure was standardized in the laboratory as per the protocol of National Hormone & Peptide Program (NHPP) and Steiner et.al., 1969  The sensitivity for cAMP assay was ~0.01 pmoles/tube and the inter- and intra-assay variations were 5.2% and 7.4% respectively. The antibody (CV-27) was used at the final assay tube dilution of 1: 200,000 in a total assay set up volume of 500 μl. The standard graph constructed with concentrations of different acetylated standard on X-axis and % binding on Y-axis was used to extrapolate concentration of cAMP in samples.
Determination of PDE activity using3HcAMP as substrate
The cAMP-PDE activity assay was carried out as reported previously with few modifications . Fresh CL tissues (8-10 mg) were homogenized in 200 μl of PDE lysis buffer and the lysate containing enzyme was incubated with cocktail containing3HcAMP substrate, 50 mM HEPES (pH 7.4) and 10 mM MgCl2 at 30°C for 15 min. The PDE reaction was stopped by incubating the tubes in 95°C hot water bath for 3 min and incubated again at 30°C for 15 min. To the reaction mixture, 10 μl (50 KU) of snake venom nucleotidase was added and incubated at 30°C for further 45 min. The reaction mixture was applied on to 1 ml syringe columns containing anion-exchange resin QAE sephadex A-25 (200-400 mesh, GE life sciences, NJ, USA) and equilibrated in 20 mM ammonium formate at pH 6.0 prior to use. The elution was carried out with 500 μl of 10 mM Tris pH 7.4, and the same was repeated for further 5 more times. The eluant was mixed with 10 ml of scintillation fluid and the dpm in each sample was counted for 5 min in liquid scintillation counter.
Total RNA was isolated from CL tissues harvested from different experiments using TRI Reagent according to manufacturer's instructions. RNA quantitation was performed using NanoDrop ND-1000 UV-VIS spectrophotometer (NanoDrop Products, Wilmington, DE) and RNA samples with A260/A280 values of ~1.8-1.9 were considered for further analysis.
Semi-quantitative RT-PCR analysis
Semi-quantitative RT-PCR analysis was carried out essentially as described previously . The details of primers employed along with the annealing temperature and expected amplicon size are provided in Additional file 1: Table S1.
Real-time quantitative RT-PCR analysis
Real-time quantitative RT-PCR (qPCR) analysis was carried out essentially as described previously . Expression of L19 was used as internal control for normalization of each gene examined. The list of genes with details of the primers employed along with the annealing temperature and expected amplicon size are provided in Additional file 2: Table S2.
Northern blot analysis
Northern blot analysis was carried out as described previously with few modifications . Briefly, total RNA (15 μg) extracted from CL were electrophoresed and blotted onto nylon membrane by capillary blotting for 16 h in 20× SSC. The blot was pre-hybridized for 2 h and hybridized with α32P-dCTP labeled LH/CGR cDNA probe for 18 h at 65°C in Church buffer . The blots were then washed sequentially with buffers containing varying concentrations of SSC + SDS and then processed for autoradiography with an intensifying screen. Hybridization signals were quantitated using a PhosphorImager (Typhoon 9210, Amersham biosciences). The equal loading of RNA samples was confirmed by reprobing the stripped blot with L-19 labelled cDNA probe.
CL tissue lysate preparation and immunoblot analysis were carried out following the previously published procedures with some modifications . Briefly, nonspecific sites on the membrane were blocked using 10% milk in TBST by incubating 1 h at room temperature instead of overnight at 4°C. Membrane was further incubated with primary antibody specific for different proteins for overnight at 4°C instead for 3 h at room temperature. The membranes were developed by ECL chemiluminescence kit from Cell Signaling Technology (Beverly, MA) instead of (NEN Life Sciences). Autoradiographs were scanned using HP scanner or Syngene gel documentation system and quantitated by densitometry using Gene tools software (Syngene laboratories, MD, USA) instead of UVI-Tech gel documentation system and quantitated using UVI-Band Map (1999) software.
Data were expressed as mean ± SEM. Statistical evaluation of mean differences in P4 concentrations and qPCR expression of genes was analysed by one-way ANOVA, followed by the Newman- Keuls multiple comparison test (PRISM GraphPad, version 5.0; GraphPad Software Inc., San Diego, CA) and Student's t-test to compare between two groups. A p value of < 0.05 was considered statistically significant.
Expression and characterization of LH/CGR transcript variants and protein in the monkey CL throughout the luteal phase
Northern blot analysis was performed for LH/CGR mRNA to examine the presence of different transcripts and their size in the monkey CL throughout the luteal phase. As seen in Figure 1B, the alternative splicing for LH/CGR mRNA was operational in the monkey CL and four transcripts were detected, one major band with an apparent size of 4.0 kb and three minor bands with sizes of 1.0, 7.5 and 8.0 kb. All the four transcripts were present in the CL tissues collected from monkeys at the late luteal phase and on day 1 of menses, but the intensity of transcripts was lower compared to mid stage (Figure 1B). Since LH/CGR mRNA expression was maximal at late luteal phase, it became of interest to examine whether any of these transcript variants were differentially regulated. The results indicated presence of similar expression of splice variants both during mid and late stage of the luteal phase (Figure 1B).
In contrast to the earlier report suggesting the absence of LH/CGR mRNA expression in the regressing monkey CL , in the present study the presence of LH/CGR mRNA expression in the regressing CL prompted us to employ multiple primer pairs PCR approach to further determine the nature of alternative splicing variants. The organization of LH/CGR gene and design of multiple primer pair sets and various predicted transcript variants lacking different exons are provided in Additional file 3: Figure S1. The sets of multiple primer pairs were designed such that both full length transcripts and splice variants would be detected. The details of primer sets, RT-PCR conditions and the expected size of splice variants are described in the Additional file 1: Table S1. Semi-quantitative RT-PCR analysis of LH/CGR mRNA demonstrates presence of two products of sizes 519 bp and 333 bp using exon 7-11(F1-R1) primer set whereas, products of sizes 718 bp and 532 bp using exon 5-11(F2-R2) primer set corresponding to a full length transcript and one splice variant lacking some exon region using both the primer sets (Figure 1C). The four cDNA products [upper (519/718 bp) and lower (333/532 bp) band using both the primer sets] were gel eluted and sequenced. The nucleotide sequences were compared with Gen Bank database using sequence alignment at BLAST (Basic Local Alignment Search Tool) and CLUSTALW tools from DNA Data Bank of Japan (DDBJ), National Center for Biological Information (NCBI) and GenomeNet  analysis showed ≥95% sequence identity to human LH/CGR and ≥99% sequence identity to monkey LH/CGR (Additional file 4: Figure S2A and B). The two bands i.e., the upper band corresponding to full length (519/718 bp) transcript and the lower band (333/532 bp) corresponding to splice variant lacking exon 9 were detected throughout the luteal phase (Figure 1C).
The role of the splice variant lacking exon 9 is unclear as while it is observed in the human ovary and binds both LH and hCG in transfected in COS-1 cells, it could not stimulate adenylate cyclase . Further, it was reported that this splice variant interacted with human follitropin receptor (hFSHR) and negatively regulated its expression by trapping them in the endoplasmic reticulum [47–49]. In the monkey CL, the steady state mRNA levels for FSHR were examined by both semi-quantitative RT-PCR and qPCR analyses throughout the luteal phase (Additional file 5: Figure S3). A primer set exon 11-11(F3-R3) designed outside the region of alternative splicing to include all splice variants of the LH/CGR and a primer set exon 9-9 (F4-R4) designed within the exon 9 to examine the expression of exon 9 containing variant. Semi-quantitative RT-PCR analysis using these primer sets amplified transcripts throughout the luteal phase, which followed the pattern of full length LH/CGR transcript expression (Figure 1C). Immunoblot analysis of LH/CGR indicated that the protein was present throughout the luteal phase including on day 1 of menses (Figure 1D). To further investigate the functionality of LH/CGR present on day 1 of menses, exogenous administration of rhLH caused significant (p < 0.05) increase in circulating P4 levels (0.23 ± 0.06 vs. 1.38 ± 0.26 ng/ml at 0 and 2 h post rhLH injection, respectively, Figure 1E).
Expression and characterization of genes belonging to SFKs and their functional activation in the monkey CL throughout the luteal phase
Expression and characterization of cAMP-PDE (PDE4D) and its functional activation in the monkey CL during the luteal phase
The role of PDE4D depends on its functional activity, and analysis of cAMP-PDE activity indicated a significantly (p < 0.05) higher activity in CL tissue collected from monkeys on day 1 of menses compared to the mid stage CL (248.76 ± 26.12 vs. 125.14 ± 32.58 pmol cAMP hydrolysed/min/mg protein in D1M and MCL, respectively; Figure 3D). Since, LH mediates its action primarily by activating classical cAMP/PKA/CREB pathway, activation of SFKs or in turn PDE4D might modulate one of these components to regulate LH signaling. The PDE4D hydrolyzes cAMP to 5'AMP and thus play a critical role in the regulation of intracellular cAMP levels. It was of interest to examine the cAMP content in CL tissues throughout the luteal phase. The cAMP content was high in mid stage compared to CL from late stage and on day 1 of menses and the data is presented in Additional file 7: Figure S5A, The findings of higher cAMP-PDE activity together with higher pSrc (Y-416) levels strongly suggest involvement of SFKs and PDE in modulation of LH actions leading to loss of function and eventually regression of CL at the end of non-fertile cycle.
Expression and characterization of LH/CGR and genes belonging to SFKs in the monkey CL during PGF2α-induced luteolysis
Expression and characterization of LH/CGR, genes belonging to SFKs and PDE4D in the CL during LH secretion inhibition and LH replacement
Expression and characterization of LH/CGR and genes belonging to SFKs in the monkey CL during simulated early pregnancy condition
Expression and characterization of genes associated with steroidogenesis/cholesterol trafficking (HMGR and SR-B1) and transcription factors (LRH-1 and SF-1) downstream of LH/CGR signaling
A model depicting the overall results showing various molecular players involved downstream of LH/CGR activation resulting in the regulation of expression of genes associated with steroidogenesis in the monkey CL
In higher primates, the mechanisms responsible for luteal regression that occurs at the end of non-fertile cycle are poorly understood. In the present studies, efforts were made to explain the dichotomy of LH/CG control of luteal function during non-fertile/fertile cycles in the monkey. We hypothesized that the signaling pathways downstream of LH/CGR may play a crucial role in modulating the LH/CG actions and in turn CL function. The results of studies carried out employing various model systems clearly suggest involvement of SFKs and cAMP-PDE in the decreased responsiveness of LH/CGR activated signaling during luteolysis. Furthermore, the results emphasize the importance of SR-B1, a membrane receptor protein, for trafficking of cholesterol to be an additional regulatory step influencing steroidogenesis in luteal cells.
In the present study, LH/CGR expression was observed to be high at the late luteal phase and the expression was detectable even on day 1 of menses in contrast to non detectable expression on day 1 of menses reported earlier by others [42, 50]. Cameron et al., 1982  reported that the decline in P4 levels seen during late luteal phase was not accompanied by appreciable decline in number and affinity of LH/CGR. The results from this study further extend that observation, since the regressing CL continues to maintain responsiveness to exogenous LH/CG stimulation. To rule out the possibility that the changes in differentially expressed variants of LH/CGR as previously reported in the monkey , human , rat  and the bovine , do not contribute to the onset of luteolysis, presence of multiple splice variants was examined employing primers designed around the hinge region of LH/CGR gene. Several studies have reported transcript variants of LH/CGR involving mutations in exon 7 and/or absence of exon 10 leading to loss of receptor expression to the plasma membrane or loss of LH binding, but not CG binding to receptor [55–57]. In the present study, expression of splice variant lacking exon 9 was characterized and this variant has previously been reported in humans  and rats . Although the presence of full-length LH/CGR transcript and the splice variant lacking exon 9 could be established in the monkey CL, we could not find the differential expression of these variants as reported for human luteal cells, in which the truncated variant has been suggested to modulate the cell surface expression and activity of full-length LH/CGR . The role of monkey LH/CGR splice variant lacking exon 9 is not clear, but as reported previously in human luteal cells, activation of the splice variant may not lead to increased adenylate cyclise activity  or may negatively modulate expression of wild type gonadotropin receptors by trapping them in the ER [47–49]. In the monkey, sequestration of gonadotropin receptors by the exon 9 lacking variant was ruled out since full length LH/CGR expression was high during the late luteal phase as shown in Figure 1B, D while the FSHR expression was low through most part of the luteal phase as shown in Additional file 5: Figure S3 indicating lack of interaction between LH/CGR transcript variant and LH/CGR or FSHR. The findings of LH/CGR expression and analysis of its transcript variants taken together suggest that the cause for decline in CL function does not appear to be due to decrease in LH/CGR expression or changes in expression pattern of LH/CGR splice variants. Surprisingly, mRNA expression of LH/CGR post LH inhibition and replacement showed lower expression but it was not reflected in the changes in protein levels. One explanation for this discrepancy is that mRNA levels are low and fluctuating in CL of VEH treated animals. Also, the antibody employed against LH/CGR detects one isoform that does not appear change irrespective of treatments. Moreover, it is possible that transcriptional changes may not be reflected in the translational changes at the time points examined.
The review of literature on effects of LH/CG suggests regulation of various functions of luteal and other steroidogenic cells involve activation of multiple signaling pathways [14, 60, 61]. Although LH mediates its action by Gs/cAMP/PKA/CREB canonical pathway, recently it has been reported that activation of LH/CGR also results in activation of other G proteins [11, 12] and receptors involving tyrosine phosphorylation leading to activation of Ras/ERK1/2 cascade [16, 20, 21, 62, 63]. Activation of ERK cascade may involve other signaling molecules such as SFKs, β-arrestins, PKA, Ras, Shc and the EGFR as intermediates in the pathway [64–69]. Studies carried out employing inhibitors and dominant negative constructs of SFKs have shown that many of these molecules interact with the canonical LH/CGR activated signaling pathway resulting in altered responsiveness to steroidogenesis in ovarian cells [19, 70, 71], MA-10 tumor Leydig cells [18, 72, 73] and adrenal cells [74, 75]. In the present study, expression of genes belonging to SFKs confirm low levels of activated Src during high steroidogenic state or increased activated levels of Src during low steroidogenic state of the CL. The interaction between the LH/CGR and SFKs can possibly either be a direct [17, 76] or indirect involving Pyk2  or β-arrestins [66, 78] to keep Src activation in check. Thus, increased Src activation observed during low P4 secretion may be due to reduced responsiveness to LH since cAMP-PDE activity involving Ras activation may be affected as observed in MA-10 and ovarian theca cells [18, 19, 73]. The role of PDE in the regulation of endocrine cell functions has been well documented [79–83]. In the present study, presence of few PDE4D isoforms in the CL tissue also as reported in other cells was confirmed [84–87]. Initial attempts to correlate increased PDE activity with Src activation was not successful as it was difficult to determine the direct/indirect interactions in the monkey CL as reported for other cell types [88–93]. Nonetheless, the results obtained employing different models systems in the present study and as hypothesized by other investigators, it can be suggested that modulation of LH/CGR signaling by Src might involve a role for PDE.
Several studies suggest important role for regulation of StAR expression by LH in primates . In species such as bovines, StAR expression appears to be central to regulation of CL function and further, it may be viewed that steroidogenesis is critically dependent on factors that regulate StAR expression . However, the results from the present study, suggest that cholesterol acquisition by luteal cells involving receptor proteins such as SR-B1 appears to be also equally important for regulation of CL function in monkeys. SR-B1 mRNA and protein levels corroborate well with the steroidogenic status of the CL as reported previously for the ovary [96, 97], adrenal [31, 98] and Leydig cells [99, 100]. Studies by others have also suggested that SR-B1 to be a key LH regulated gene modulating steroidogenic activity as observed in the present study [27, 96]. Moreover, presence of orphan nuclear transcription factors such as LRH-1 and SF-1 expression in the monkey CL may suggest transcriptional regulation of SR-B1 expression as observed in the bovine CL . In summary, based on the results of the present study, at least four possible scenarios represented pictorially in Figure 8 can be thought of to explain the regulation of CL function during different functional status. They are: 1) CL during maximal steroidogenic state (mid luteal phase) and consequently high circulating P4 levels. Upon activation of Gs/cAMP/PKA/CREB pathway by LH leads to increased expression of genes such as SR-B1. Further, presence of the decreased activated pSrc (Tyr-416) level indicates LH signaling to be inhibitory to Src activation and lowered cAMP-PDE activity. 2) CL during spontaneous luteolysis and/or following PGF2α-induced luteolysis is concordant with lowered circulating P4 levels. The LH canonical signaling pathway is modulated by increased activated pSrc (Tyr-416) levels and cAMP-PDE activity leading to lowered cAMP levels, PKA activity and pCREB levels which corroborate well with decreased expression of SR-B1. 3) CL during decreased luteal function due to inhibition of circulating LH by CET treatment leading to low circulating P4 levels. The inhibition of LH canonical signaling pathway leads to increase in activated pSrc (Tyr-416) levels and cAMP-PDE activity with concomitant decrease in SR-B1 expression, and 4) CL during simulated early pregnancy condition and after LH replacement in the CET-treated monkeys causing higher or rescued function of CL resulting in higher circulating P4 levels. The Gs/cAMP/PKA/CREB pathway is robust with higher cAMP levels, increased PKA activity and increased pCREB expression consequently decreased activated pSrc (Tyr-416) and cAMP-PDE levels, finally leading to higher SR-B1 expression.
The results indicate participation of activated Src [pSrc (Tyr-416)] and increased activity of cAMP-PDE during spontaneous luteolysis, while LH/hCG treatment caused decreased activation of Src and cAMP-PDE activity concomitant with increased secretion of P4 in the CL.
We are grateful to Dr. Basavanagouda and staff of Primate Research Laboratory for assistance with surgeries. This work was supported by the Department of Biotechnology, India [BT/IN/US/CRHR/8JWG/2006].
- Bazer FW, Wu G, Spencer TE, Johnson GA, Burghardt RC, Bayless K: Novel pathways for implantation and establishment and maintenance of pregnancy in mammals. Mol Hum Reprod. 2010, 16 (3): 135-152. 10.1093/molehr/gap095.PubMed CentralView ArticlePubMedGoogle Scholar
- Stocco C, Telleria C, Gibori G: The molecular control of corpus luteum formation, function, and regression. Endocr Rev. 2007, 28 (1): 117-149.View ArticlePubMedGoogle Scholar
- Moudgal NR, Macdonald GJ, Greep RO: Role of endogenous primate LH in maintaining corpus luteum function in the monkey. J Clin Endocrinol Metab. 1972, 35 (1): 113-116. 10.1210/jcem-35-1-113.View ArticlePubMedGoogle Scholar
- Hutchison JS, Zeleznik AJ: The corpus luteum of the primate menstrual cycle is capable of recovering from a transient withdrawal of pituitary gonadotropin support. Endocrinology. 1985, 117 (3): 1043-1049. 10.1210/endo-117-3-1043.View ArticlePubMedGoogle Scholar
- Yadav VK, Muraly P, Medhamurthy R: Identification of novel genes regulated by LH in the primate corpus luteum: insight into their regulation during the late luteal phase. Mol Hum Reprod. 2004, 10 (9): 629-639. 10.1093/molehr/gah089.View ArticlePubMedGoogle Scholar
- Neill JD, Knobil E: On the nature of the initial luteotropic stimulus of pregnancy in the Rhesus monkey. Endocrinology. 1972, 90 (1): 34-38. 10.1210/endo-90-1-34.View ArticlePubMedGoogle Scholar
- Becker J, Walz A, Daube S, Keck C, Pietrowski D: Distinct responses of human granulosa lutein cells after hCG or LH stimulation in a spheroidal cell culture system. Mol Reprod Dev. 2007, 74 (10): 1312-1316. 10.1002/mrd.20696.View ArticlePubMedGoogle Scholar
- Walz A, Keck C, Weber H, Kissel C, Pietrowski D: Effects of luteinizing hormone and human chorionic gonadotropin on corpus luteum cells in a spheroid cell culture system. Mol Reprod Dev. 2005, 72 (1): 98-104. 10.1002/mrd.20325.View ArticlePubMedGoogle Scholar
- Ascoli M, Fanelli F, Segaloff DL: The lutropin/choriogonadotropin receptor, a 2002 perspective. Endocr Rev. 2002, 23 (2): 141-174. 10.1210/er.23.2.141.View ArticlePubMedGoogle Scholar
- Hirakawa T, Galet C, Ascoli M: MA-10 cells transfected with the human lutropin/choriogonadotropin receptor (hLHR): a novel experimental paradigm to study the functional properties of the hLHR. Endocrinology. 2002, 143 (3): 1026-1035. 10.1210/en.143.3.1026.View ArticlePubMedGoogle Scholar
- Herrlich A, Kuhn B, Grosse R, Schmid A, Schultz G, Gudermann T: Involvement of Gs and Gi proteins in dual coupling of the luteinizing hormone receptor to adenylyl cyclase and phospholipase C. J Biol Chem. 1996, 271 (28): 16764-16772. 10.1074/jbc.271.28.16764.View ArticlePubMedGoogle Scholar
- Kuhn B, Gudermann T: The luteinizing hormone receptor activates phospholipase C via preferential coupling to Gi2. Biochemistry. 1999, 38 (38): 12490-12498. 10.1021/bi990755m.View ArticlePubMedGoogle Scholar
- Priyanka S, Medhamurthy R: Characterization of cAMP/PKA/CREB signaling cascade in the bonnet monkey corpus luteum: expressions of inhibin-alpha and StAR during different functional status. Mol Hum Reprod. 2007, 13 (6): 381-390. 10.1093/molehr/gam015.View ArticlePubMedGoogle Scholar
- Richards JS: New signaling pathways for hormones and cyclic adenosine 3',5'-monophosphate action in endocrine cells. Mol Endocrinol. 2001, 15 (2): 209-218. 10.1210/me.15.2.209.PubMedGoogle Scholar
- Yadav VK, Medhamurthy R: Dynamic changes in mitogen-activated protein kinase (MAPK) activities in the corpus luteum of the bonnet monkey (Macaca radiata) during development, induced luteolysis, and simulated early pregnancy: a role for p38 MAPK in the regulation of luteal function. Endocrinology. 2006, 147 (4): 2018-2027.View ArticlePubMedGoogle Scholar
- Gavi S, Shumay E, Wang HY, Malbon CC: G-protein-coupled receptors and tyrosine kinases: crossroads in cell signaling and regulation. Trends Endocrinol Metab. 2006, 17 (2): 48-54. 10.1016/j.tem.2006.01.006.View ArticlePubMedGoogle Scholar
- Luttrell DK, Luttrell LM: Not so strange bedfellows: G-protein-coupled receptors and Src family kinases. Oncogene. 2004, 23 (48): 7969-7978. 10.1038/sj.onc.1208162.View ArticlePubMedGoogle Scholar
- Taylor CC, Limback D, Terranova PF: Src tyrosine kinase activity is related to luteinizing hormone responsiveness: genetic manipulations using mouse MA10 Leydig cells. Endocrinology. 1996, 137 (12): 5735-5738. 10.1210/en.137.12.5735.PubMedGoogle Scholar
- Taylor CC, Limback D, Terranova PF: Src tyrosine kinase activity in rat thecal-interstitial cells and mouse TM3 Leydig cells is positively associated with cAMP-specific phosphodiesterase activity. Mol Cell Endocrinol. 1997, 126 (1): 91-100. 10.1016/S0303-7207(96)03975-5.View ArticlePubMedGoogle Scholar
- Mizutani T, Shiraishi K, Welsh T, Ascoli M: Activation of the lutropin/choriogonadotropin receptor in MA-10 cells leads to the tyrosine phosphorylation of the focal adhesion kinase by a pathway that involves Src family kinases. Mol Endocrinol. 2006, 20 (3): 619-630.PubMed CentralView ArticlePubMedGoogle Scholar
- Shiraishi K, Ascoli M: Activation of the lutropin/choriogonadotropin receptor in MA-10 cells stimulates tyrosine kinase cascades that activate ras and the extracellular signal regulated kinases (ERK1/2). Endocrinology. 2006, 147 (7): 3419-3427. 10.1210/en.2005-1478.PubMed CentralView ArticlePubMedGoogle Scholar
- Liscum L, Munn NJ: Intracellular cholesterol transport. Biochim Biophys Acta. 1999, 1438 (1): 19-37.View ArticlePubMedGoogle Scholar
- Luo L, Chen H, Stocco DM, Zirkin BR: Leydig cell protein synthesis and steroidogenesis in response to acute stimulation by luteinizing hormone in rats. Biol Reprod. 1998, 59 (2): 263-270. 10.1095/biolreprod59.2.263.View ArticlePubMedGoogle Scholar
- Stocco DM: Tracking the role of a star in the sky of the new millennium. Mol Endocrinol. 2001, 15 (8): 1245-1254. 10.1210/me.15.8.1245.View ArticlePubMedGoogle Scholar
- Andersen JM, Dietschy JM: Relative importance of high and low density lipoproteins in the regulation of cholesterol synthesis in the adrenal gland, ovary, and testis of the rat. J Biol Chem. 1978, 253 (24): 9024-9032.PubMedGoogle Scholar
- Spady DK, Dietschy JM: Sterol synthesis in vivo in 18 tissues of the squirrel monkey, guinea pig, rabbit, hamster, and rat. J Lipid Res. 1983, 24 (3): 303-315.PubMedGoogle Scholar
- Azhar S, Nomoto A, Leers-Sucheta S, Reaven E: Simultaneous induction of an HDL receptor protein (SR-BI) and the selective uptake of HDL-cholesteryl esters in a physiologically relevant steroidogenic cell model. J Lipid Res. 1998, 39 (8): 1616-1628.PubMedGoogle Scholar
- Connelly MA, Klein SM, Azhar S, Abumrad NA, Williams DL: Comparison of class B scavenger receptors, CD36 and scavenger receptor BI (SR-BI), shows that both receptors mediate high density lipoprotein-cholesteryl ester selective uptake but SR-BI exhibits a unique enhancement of cholesteryl ester uptake. J Biol Chem. 1999, 274 (1): 41-47. 10.1074/jbc.274.1.41.View ArticlePubMedGoogle Scholar
- Azhar S, Reaven E: Scavenger receptor class BI and selective cholesteryl ester uptake: partners in the regulation of steroidogenesis. Mol Cell Endocrinol. 2002, 195 (1-2): 1-26. 10.1016/S0303-7207(02)00222-8.View ArticlePubMedGoogle Scholar
- Azhar S, Leers-Sucheta S, Reaven E: Cholesterol uptake in adrenal and gonadal tissues: the SR-BI and 'selective' pathway connection. Front Biosci. 2003, 8: s998-s1029. 10.2741/1165.View ArticlePubMedGoogle Scholar
- Connelly MA, Williams DL: SR-BI and cholesterol uptake into steroidogenic cells. Trends Endocrinol Metab. 2003, 14 (10): 467-472. 10.1016/j.tem.2003.10.002.View ArticlePubMedGoogle Scholar
- Taniguchi H, Komiyama J, Viger RS, Okuda K: The expression of the nuclear receptors NR5A1 and NR5A2 and transcription factor GATA6 correlates with steroidogenic gene expression in the bovine corpus luteum. Mol Reprod Dev. 2009, 76 (9): 873-880. 10.1002/mrd.21054.View ArticlePubMedGoogle Scholar
- Schoonjans K, Annicotte JS, Huby T, Botrugno OA, Fayard E, Ueda Y, Chapman J, Auwerx J: Liver receptor homolog 1 controls the expression of the scavenger receptor class B type I. EMBO Rep. 2002, 3 (12): 1181-1187. 10.1093/embo-reports/kvf238.PubMed CentralView ArticlePubMedGoogle Scholar
- Priyanka S, Jayaram P, Sridaran R, Medhamurthy R: Genome-wide gene expression analysis reveals a dynamic interplay between luteotropic and luteolytic factors in the regulation of corpus luteum function in the bonnet monkey (Macaca radiata). Endocrinology. 2009, 150 (3): 1473-1484.PubMed CentralView ArticlePubMedGoogle Scholar
- Rao CV, Ireland JJ, Roche JF: Decrease of various luteal enzyme activities during prostaglandin F2 alpha-induced luteal regression in bovine. Mol Cell Endocrinol. 1984, 34 (2): 99-105. 10.1016/0303-7207(84)90060-1.View ArticlePubMedGoogle Scholar
- Selvaraj N, Medhamurthy R, Ramachandra SG, Sairam MR, Moudgal NR: Assessment of luteal rescue and desensitization of macaque corpus luteum brought about by human chorionic gonadotrophin and deglycosylated human chorionic gonadotrophin treatment. J Biosci. 1996, 21 (4): 497-510. 10.1007/BF02703214.View ArticleGoogle Scholar
- Steiner AL, Kipnis DM, Utiger R, Parker C: Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate. Proc Natl Acad Sci USA. 1969, 64 (1): 367-373. 10.1073/pnas.64.1.367.PubMed CentralView ArticlePubMedGoogle Scholar
- Thompson WJ, Appleman MM: Multiple cyclic nucleotide phosphodiesterase activities from rat brain. Biochemistry. 1971, 10 (2): 311-316. 10.1021/bi00778a018.View ArticlePubMedGoogle Scholar
- Jyotsna UR, Medhamurthy R: Standardization and validation of an induced ovulation model system in buffalo cows: Characterization of gene expression changes in the periovulatory follicle. Anim Reprod Sci. 2009, 113 (1-4): 71-81. 10.1016/j.anireprosci.2008.08.001.View ArticlePubMedGoogle Scholar
- Yadav VK, Sudhagar RR, Medhamurthy R: Apoptosis during spontaneous and prostaglandin F(2alpha)-induced luteal regression in the buffalo cow (Bubalus bubalis): involvement of mitogen-activated protein kinases. Biol Reprod. 2002, 67 (3): 752-759. 10.1095/biolreprod.102.004077.View ArticlePubMedGoogle Scholar
- Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA. 1984, 81 (7): 1991-1995. 10.1073/pnas.81.7.1991.PubMed CentralView ArticlePubMedGoogle Scholar
- Ravindranath N, Little-Ihrig LL, Zeleznik AJ: Characterization of the levels of messenger ribonucleic acid that encode for luteinizing hormone receptor during the luteal phase of the primate menstrual cycle. J Clin Endocrinol Metab. 1992, 74 (4): 779-785. 10.1210/jc.74.4.779.View ArticlePubMedGoogle Scholar
- DNA Data Bank of Japan. [http://blast.ddbj.nig.ac.jp/]
- National center for Biological information. [http://blast.ncbi.nlm.nih.gov/Blast.cgi]
- GenomeNet. [http://www.genome.jp/tools/clustalw/]
- Minegishi T, Tano M, Abe Y, Nakamura K, Ibuki Y, Miyamoto K: Expression of luteinizing hormone/human chorionic gonadotrophin (LH/HCG) receptor mRNA in the human ovary. Mol Hum Reprod. 1997, 3 (2): 101-107. 10.1093/molehr/3.2.101.View ArticlePubMedGoogle Scholar
- Minegishi T, Nakamura K, Yamashita S, Omori Y: The effect of splice variant of the human luteinizing hormone (LH) receptor on the expression of gonadotropin receptor. Mol Cell Endocrinol. 2007, 260-262: 117-125.View ArticlePubMedGoogle Scholar
- Yamashita S, Nakamura K, Omori Y, Tsunekawa K, Murakami M, Minegishi T: Association of human follitropin (FSH) receptor with splicing variant of human lutropin/choriogonadotropin receptor negatively controls the expression of human FSH receptor. Mol Endocrinol. 2005, 19 (8): 2099-2111. 10.1210/me.2005-0049.View ArticlePubMedGoogle Scholar
- Nakamura K, Yamashita S, Omori Y, Minegishi T: A splice variant of the human luteinizing hormone (LH) receptor modulates the expression of wild-type human LH receptor. Mol Endocrinol. 2004, 18 (6): 1461-1470. 10.1210/me.2003-0489.View ArticlePubMedGoogle Scholar
- Nishimori K, Dunkel L, Hsueh AJ, Yamoto M, Nakano R: Expression of luteinizing hormone and chorionic gonadotropin receptor messenger ribonucleic acid in human corpora lutea during menstrual cycle and pregnancy. J Clin Endocrinol Metab. 1995, 80 (4): 1444-1448. 10.1210/jc.80.4.1444.PubMedGoogle Scholar
- Cameron JL, Stouffer RL: Gonadotropin receptors of the primate corpus luteum. II. Changes in available luteinizing hormone- and chorionic gonadotropin-binding sites in macaque luteal membranes during the nonfertile menstrual cycle. Endocrinology. 1982, 110 (6): 2068-2073. 10.1210/endo-110-6-2068.View ArticlePubMedGoogle Scholar
- Madhra M, Gay E, Fraser HM, Duncan WC: Alternative splicing of the human luteal LH receptor during luteolysis and maternal recognition of pregnancy. Mol Hum Reprod. 2004, 10 (8): 599-603. 10.1093/molehr/gah076.View ArticlePubMedGoogle Scholar
- Wang H, Ascoli M, Segaloff DL: Multiple luteinizing hormone/chorionic gonadotropin receptor messenger ribonucleic acid transcripts. Endocrinology. 1991, 129 (1): 133-138. 10.1210/endo-129-1-133.View ArticlePubMedGoogle Scholar
- Smith GD, Sawyer HR, Mirando MA, Griswold MD, Sadhu A, Reeves JJ: Steady-state luteinizing hormone receptor messenger ribonucleic acid levels and endothelial cell composition in bovine normal- and short-lived corpora lutea. Biol Reprod. 1996, 55 (4): 902-909. 10.1095/biolreprod55.4.902.View ArticlePubMedGoogle Scholar
- Gromoll J, Wistuba J, Terwort N, Godmann M, Muller T, Simoni M: A new subclass of the luteinizing hormone/chorionic gonadotropin receptor lacking exon 10 messenger RNA in the New World monkey (Platyrrhini) lineage. Biol Reprod. 2003, 69 (1): 75-80. 10.1095/biolreprod.102.014902.View ArticlePubMedGoogle Scholar
- Muller T, Gromoll J, Simoni M: Absence of exon 10 of the human luteinizing hormone (LH) receptor impairs LH, but not human chorionic gonadotropin action. J Clin Endocrinol Metab. 2003, 88 (5): 2242-2249. 10.1210/jc.2002-021946.View ArticlePubMedGoogle Scholar
- Gromoll J, Schulz A, Borta H, Gudermann T, Teerds KJ, Greschniok A, Nieschlag E, Seif FJ: Homozygous mutation within the conserved Ala-Phe-Asn-Glu-Thr motif of exon 7 of the LH receptor causes male pseudohermaphroditism. Eur J Endocrinol. 2002, 147 (5): 597-608. 10.1530/eje.0.1470597.View ArticlePubMedGoogle Scholar
- Aatsinki JT, Pietila EM, Lakkakorpi JT, Rajaniemi HJ: Expression of the LH/CG receptor gene in rat ovarian tissue is regulated by an extensive alternative splicing of the primary transcript. Mol Cell Endocrinol. 1992, 84 (1-2): 127-135. 10.1016/0303-7207(92)90079-L.View ArticlePubMedGoogle Scholar
- Dickinson RE, Stewart AJ, Myers M, Millar RP, Duncan WC: Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology. 2009, 150 (6): 2873-2881. 10.1210/en.2008-1382.View ArticlePubMedGoogle Scholar
- Carvalho CR, Carvalheira JB, Lima MH, Zimmerman SF, Caperuto LC, Amanso A, Gasparetti AL, Meneghetti V, Zimmerman LF, Velloso LA, et al: Novel signal transduction pathway for luteinizing hormone and its interaction with insulin: activation of Janus kinase/signal transducer and activator of transcription and phosphoinositol 3-kinase/Akt pathways. Endocrinology. 2003, 144 (2): 638-647. 10.1210/en.2002-220706.View ArticlePubMedGoogle Scholar
- Davis JS: Mechanisms of hormone action: luteinizing hormone receptors and second-messenger pathways. Curr Opin Obstet Gynecol. 1994, 6 (3): 254-261.View ArticlePubMedGoogle Scholar
- Conti M, Hsieh M, Park JY, Su YQ: Role of the epidermal growth factor network in ovarian follicles. Mol Endocrinol. 2006, 20 (4): 715-723.View ArticlePubMedGoogle Scholar
- Hsieh M, Conti M: G-protein-coupled receptor signaling and the EGF network in endocrine systems. Trends Endocrinol Metab. 2005, 16 (7): 320-326. 10.1016/j.tem.2005.07.005.View ArticlePubMedGoogle Scholar
- Tai P, Shiraishi K, Ascoli M: Activation of the lutropin/choriogonadotropin receptor inhibits apoptosis of immature Leydig cells in primary culture. Endocrinology. 2009, 150 (8): 3766-3773. 10.1210/en.2009-0207.PubMed CentralView ArticlePubMedGoogle Scholar
- Shiraishi K, Ascoli M: Lutropin/choriogonadotropin stimulate the proliferation of primary cultures of rat Leydig cells through a pathway that involves activation of the extracellularly regulated kinase 1/2 cascade. Endocrinology. 2007, 148 (7): 3214-3225. 10.1210/en.2007-0160.PubMed CentralView ArticlePubMedGoogle Scholar
- Galet C, Ascoli M: Arrestin-3 is essential for the activation of Fyn by the luteinizing hormone receptor (LHR) in MA-10 cells. Cell Signal. 2008, 20 (10): 1822-1829. 10.1016/j.cellsig.2008.06.005.PubMed CentralView ArticlePubMedGoogle Scholar
- Andric N, Ascoli M: The luteinizing hormone receptor-activated extracellularly regulated kinase-1/2 cascade stimulates epiregulin release from granulosa cells. Endocrinology. 2008, 149 (11): 5549-5556. 10.1210/en.2008-0618.PubMed CentralView ArticlePubMedGoogle Scholar
- Shiraishi K, Ascoli M: A co-culture system reveals the involvement of intercellular pathways as mediators of the lutropin receptor (LHR)-stimulated ERK1/2 phosphorylation in Leydig cells. Exp Cell Res. 2008, 314 (1): 25-37. 10.1016/j.yexcr.2007.06.025.PubMed CentralView ArticlePubMedGoogle Scholar
- Panigone S, Hsieh M, Fu M, Persani L, Conti M: Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Mol Endocrinol. 2008, 22 (4): 924-936. 10.1210/me.2007-0246.PubMed CentralView ArticlePubMedGoogle Scholar
- Chaturvedi G, Arai K, Terranova PF, Roby KF: The Src tyrosine kinase pathway regulates thecal CYP17 expression and androstenedione secretion. Mol Cell Biochem. 2008, 318 (1-2): 191-200. 10.1007/s11010-008-9871-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Taylor CC, Terranova PF: Lipopolysaccharide inhibits rat ovarian thecal-interstitial cell steroid secretion in vitro. Endocrinology. 1995, 136 (12): 5527-5532. 10.1210/en.136.12.5527.PubMedGoogle Scholar
- Roby KF, Son DS, Taylor CC, Montgomery-Rice V, Kirchoff J, Tang S, Terranova PF: Alterations in reproductive function in SRC tyrosine kinase knockout mice. Endocrine. 2005, 26 (2): 169-176. 10.1385/ENDO:26:2:169.View ArticlePubMedGoogle Scholar
- Taylor CC: Src tyrosine kinase-induced loss of luteinizing hormone responsiveness is via a Ras-dependent, phosphatidylinositol-3-kinase independent pathway. Biol Reprod. 2002, 67 (3): 789-794. 10.1095/biolreprod.101.000976.View ArticlePubMedGoogle Scholar
- Sirianni R, Carr BR, Pezzi V, Rainey WE: A role for src tyrosine kinase in regulating adrenal aldosterone production. J Mol Endocrinol. 2001, 26 (3): 207-215. 10.1677/jme.0.0260207.View ArticlePubMedGoogle Scholar
- Sirianni R, Carr BR, Ando S, Rainey WE: Inhibition of Src tyrosine kinase stimulates adrenal androgen production. J Mol Endocrinol. 2003, 30 (3): 287-299. 10.1677/jme.0.0300287.View ArticlePubMedGoogle Scholar
- Ma YC, Huang J, Ali S, Lowry W, Huang XY: Src tyrosine kinase is a novel direct effector of G proteins. Cell. 2000, 102 (5): 635-646. 10.1016/S0092-8674(00)00086-6.View ArticlePubMedGoogle Scholar
- Dikic I, Tokiwa G, Lev S, Courtneidge SA, Schlessinger J: A role for Pyk2 and Src in linking G-protein-coupled receptors with MAP kinase activation. Nature. 1996, 383 (6600): 547-550. 10.1038/383547a0.View ArticlePubMedGoogle Scholar
- Luttrell LM, Ferguson SS, Daaka Y, Miller WE, Maudsley S, Della Rocca GJ, Lin F, Kawakatsu H, Owada K, Luttrell DK, et al: Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Science. 1999, 283 (5402): 655-661. 10.1126/science.283.5402.655.View ArticlePubMedGoogle Scholar
- Conti M, Beavo J: Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling. Annu Rev Biochem. 2007, 76: 481-511. 10.1146/annurev.biochem.76.060305.150444.View ArticlePubMedGoogle Scholar
- Mehats C, Andersen CB, Filopanti M, Jin SL, Conti M: Cyclic nucleotide phosphodiesterases and their role in endocrine cell signaling. Trends Endocrinol Metab. 2002, 13 (1): 29-35. 10.1016/S1043-2760(01)00523-9.View ArticlePubMedGoogle Scholar
- Conti M: Phosphodiesterases and cyclic nucleotide signaling in endocrine cells. Mol Endocrinol. 2000, 14 (9): 1317-1327. 10.1210/me.14.9.1317.View ArticlePubMedGoogle Scholar
- Swinnen JV, Joseph DR, Conti M: The mRNA encoding a high-affinity cAMP phosphodiesterase is regulated by hormones and cAMP. Proc Natl Acad Sci USA. 1989, 86 (21): 8197-8201. 10.1073/pnas.86.21.8197.PubMed CentralView ArticlePubMedGoogle Scholar
- Stouffer RL, Ottobre JS, Molskness TA, Zelinski-Wooten MB: The function and regulation of the primate corpus luteum during the fertile menstrual cycle. Prog Clin Biol Res. 1989, 294: 129-142.PubMedGoogle Scholar
- Chandrasekaran A, Toh KY, Low SH, Tay SK, Brenner S, Goh DL: Identification and characterization of novel mouse PDE4D isoforms: molecular cloning, subcellular distribution and detection of isoform-specific intracellular localization signals. Cell Signal. 2008, 20 (1): 139-153. 10.1016/j.cellsig.2007.10.003.View ArticlePubMedGoogle Scholar
- Richter W, Jin SL, Conti M: Splice variants of the cyclic nucleotide phosphodiesterase PDE4D are differentially expressed and regulated in rat tissue. Biochem J. 2005, 388 (Pt 3): 803-811.PubMed CentralView ArticlePubMedGoogle Scholar
- Conti M, Richter W, Mehats C, Livera G, Park JY, Jin C: Cyclic AMP-specific PDE4 phosphodiesterases as critical components of cyclic AMP signaling. J Biol Chem. 2003, 278 (8): 5493-5496. 10.1074/jbc.R200029200.View ArticlePubMedGoogle Scholar
- Bolger GB, Erdogan S, Jones RE, Loughney K, Scotland G, Hoffmann R, Wilkinson I, Farrell C, Houslay MD: Characterization of five different proteins produced by alternatively spliced mRNAs from the human cAMP-specific phosphodiesterase PDE4D gene. Biochem J. 1997, 328 (Pt 2): 539-548.PubMed CentralView ArticlePubMedGoogle Scholar
- Beard MB, O'Connell JC, Bolger GB, Houslay MD: The unique N-terminal domain of the cAMP phosphodiesterase PDE4D4 allows for interaction with specific SH3 domains. FEBS Lett. 1999, 460 (1): 173-177. 10.1016/S0014-5793(99)01335-6.View ArticlePubMedGoogle Scholar
- O'Connell JC, McCallum JF, McPhee I, Wakefield J, Houslay ES, Wishart W, Bolger G, Frame M, Houslay MD: The SH3 domain of Src tyrosyl protein kinase interacts with the N-terminal splice region of the PDE4A cAMP-specific phosphodiesterase RPDE-6 (RNPDE4A5). Biochem J. 1996, 318 (Pt 1): 255-261.PubMed CentralView ArticlePubMedGoogle Scholar
- Dodge-Kafka KL, Bauman A, Mayer N, Henson E, Heredia L, Ahn J, McAvoy T, Nairn AC, Kapiloff MS: cAMP-stimulated protein phosphatase 2A activity associated with muscle A kinase-anchoring protein (mAKAP) signaling complexes inhibits the phosphorylation and activity of the cAMP-specific phosphodiesterase PDE4D3. J Biol Chem. 2010, 285 (15): 11078-11086. 10.1074/jbc.M109.034868.PubMed CentralView ArticlePubMedGoogle Scholar
- Carlisle Michel JJ, Dodge KL, Wong W, Mayer NC, Langeberg LK, Scott JD: PKA-phosphorylation of PDE4D3 facilitates recruitment of the mAKAP signalling complex. Biochem J. 2004, 381 (Pt 3): 587-592.PubMed CentralView ArticlePubMedGoogle Scholar
- Dodge KL, Khouangsathiene S, Kapiloff MS, Mouton R, Hill EV, Houslay MD, Langeberg LK, Scott JD: mAKAP assembles a protein kinase A/PDE4 phosphodiesterase cAMP signaling module. EMBO J. 2001, 20 (8): 1921-1930. 10.1093/emboj/20.8.1921.PubMed CentralView ArticlePubMedGoogle Scholar
- Willoughby D, Wong W, Schaack J, Scott JD, Cooper DM: An anchored PKA and PDE4 complex regulates subplasmalemmal cAMP dynamics. EMBO J. 2006, 25 (10): 2051-2061. 10.1038/sj.emboj.7601113.PubMed CentralView ArticlePubMedGoogle Scholar
- Devoto L, Kohen P, Vega M, Castro O, Gonzalez RR, Retamales I, Carvallo P, Christenson LK, Strauss JF: Control of human luteal steroidogenesis. Mol Cell Endocrinol. 2002, 186 (2): 137-141. 10.1016/S0303-7207(01)00654-2.View ArticlePubMedGoogle Scholar
- Manna PR, Dyson MT, Stocco DM: Regulation of the steroidogenic acute regulatory protein gene expression: present and future perspectives. Mol Hum Reprod. 2009, 15 (6): 321-333. 10.1093/molehr/gap025.PubMed CentralView ArticlePubMedGoogle Scholar
- Reaven E, Nomoto A, Leers-Sucheta S, Temel R, Williams DL, Azhar S: Expression and microvillar localization of scavenger receptor, class B, type I (a high density lipoprotein receptor) in luteinized and hormone-desensitized rat ovarian models. Endocrinology. 1998, 139 (6): 2847-2856. 10.1210/en.139.6.2847.PubMedGoogle Scholar
- Li X, Peegel H, Menon KM: In situ hybridization of high density lipoprotein (scavenger, type 1) receptor messenger ribonucleic acid (mRNA) during folliculogenesis and luteinization: evidence for mRNA expression and induction by human chorionic gonadotropin specifically in cell types that use cholesterol for steroidogenesis. Endocrinology. 1998, 139 (7): 3043-3049. 10.1210/en.139.7.3043.PubMedGoogle Scholar
- Connelly MA: SR-BI-mediated HDL cholesteryl ester delivery in the adrenal gland. Mol Cell Endocrinol. 2009, 300: (1-2):83-88. 10.1016/j.mce.2008.11.023.View ArticleGoogle Scholar
- Rao RM, Jo Y, Leers-Sucheta S, Bose HS, Miller WL, Azhar S, Stocco DM: Differential regulation of steroid hormone biosynthesis in R2C and MA-10 Leydig tumor cells: role of SR-B1-mediated selective cholesteryl ester transport. Biol Reprod. 2003, 68 (1): 114-121.View ArticlePubMedGoogle Scholar
- Reaven E, Zhan L, Nomoto A, Leers-Sucheta S, Azhar S: Expression and microvillar localization of scavenger receptor class B, type I (SR-BI) and selective cholesteryl ester uptake in Leydig cells from rat testis. J Lipid Res. 2000, 41 (3): 343-356.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.