Figure 3
Expression and characterization of cAMP-PDE (PDE4D) and its functional activation in the monkey CL throughout the luteal phase. (A) The qPCR expression of PDE4D mRNA, an ovarian specific cAMP-PDE in the monkey CL obtained from different stages of the luteal phase. The relative mRNA expression at each stage is represented in each bar as mean ± SEM values (n = 3 CL/stage).Bars with different letters indicate statistical significance (p < 0.05). (B) To standardize PDE4D protein analysis conditions in the monkey CL, immunoblot analysis with anti-PDE4D was performed employing brain and CL tissue lysates from various species. (C) Levels of PDE4D protein in the monkey CL throughout the luteal phase.. Anti-β-actin antibody (the protein loading control) probed blot is presented to indicate equal loading of protein in each lane. Densitometric analysis of immunoblots was determined and relative protein levels are indicated as mean ± SEM values below individual bands. (D) Determination of cAMP-PDE activity in CL tissue lysates obtained at mid stage (M) and on day 1 of menses (D1M). Each bar represents mean ± SEM values (n = 3 CL/stage). Bars with different letters indicate statistical significance (p < 0.05).