Figure 4
Expression and characterization of LH/CGR and genes belonging to SFKs in the monkey CL during PGF 2α -induced luteolysis. (A) Circulating P4 levels 24 h after VEH/PGF2α treatment. Each bar represents mean ± SEM values (n = 3 animals/treatment). (B) The fold change in qPCR expression for LH/CGR mRNA in the CL 24 h post VEH/PGF2α treatment. Each bar represents mean ± SEM values (n = 3 animals/treatment). (C) Semi-quantitative RT-PCR expression of genes belonging to SFKs (Fyn, Yes and Src) in the monkey CL obtained 24 h post VEH/PGF2α treatment. L-19 mRNA was used as internal control and the relative expression was calculated following densitometric analysis. Each bar represents mean ± SEM values (n = 3 animals/treatment). (D) Levels of LH/CGR, active pSrc (Y-416) and PDE4D protein in the monkey CL obtained 24 h post VEH/PGF2α treatment. Anti-β-actin antibody (the protein loading control) probed blot is presented to indicate equal loading of protein in each lane.