Effect of embryo morphology and morphometrics on implantation of vitrified day 3 embryos after warming: a retrospective cohort study
© The Author(s). 2016
Received: 8 July 2016
Accepted: 22 July 2016
Published: 30 July 2016
Characteristics routinely used to evaluate embryo quality after thawing include number of blastomeres survived and presence of mitosis resumption after overnight culture. It is unknown to which extent symmetry and fragmentation affect implantation after warming and whether application of stricter criteria either before vitrification or after warming would improve implantation rate (IR) of vitrified/warmed embryos. This study aimed to find new parameters to improve selection criteria for vitrification and for transfer after warming.
Firstly, we evaluated standard morphological characteristics (intact survival, mitosis resumption, number of blastomeres, symmetry and fragmentation) of 986 warmed day 3 embryos and, from a subset of 654, we evaluated morphometric characteristics (fragmentation, symmetry and volume change). Secondly, we tested the hypothesis that IR of day 3 vitrified/warmed embryos is influenced by morphometric characteristics. IR per embryo transferred was calculated using embryos that were transferred in a single embryo transfer (SET) or a double embryo transfer (DET) with either 0 or 100 % implantation (830/986). We investigated the significant differences in IR between the different categories of a specific characteristic. These categories were based on our standard embryo evaluation system. The statistical tests Chi-square, Fisher’s exact or Cochrane-Armitage were used according to the type and/or categories of the variable.
The 986 embryos were transferred in 671 FET cycles with 16.9 % (167/986) IR. After exclusion of DET with 1 embryo implanted, IR per embryo transferred was 12.4 % (103/830). Embryo symmetry, fragmentation and volume change in vitrified/warmed day 3 embryos were not associated with IR. However, when mitosis resumption was present after overnight culture, intact embryos reached significantly higher IR than non-intact embryos and only when the embryo compacted after overnight culture the number of cells damaged after warming had no effect on IR. Concretely, embryos with 8 cells after warming or >9 cells after overnight culture–including compacted embryos–reached the highest IR (>15 %) while embryos with <6 cells after warming or with ≤6 cells after overnight culture had extremely low IR (<1 %).
IR of vitrified embryos is determined by the number of cells lost, by the occurrence of mitosis resumption, and by the specific number of blastomeres present but not by fragmentation, blastomere symmetry or volume change. Unselecting embryos for cryopreservation because of fragmentation >10 % and/or symmetry < 75 % only leads to unwanted loss of embryos with acceptable implantation potential.
Retrospectively registered NCT02639715.
KeywordsVitrified/warmed embryo Embryo morphology Morphometrics Frozen embryo transfer Implantation rate
Embryo cryopreservation is an essential part of treatment with Assisted Reproductive Technology (ART). Two embryo cryopreservation methods are commonly used: slow freezing and vitrification. Vitrification has progressively substituted slow freezing in the in vitro fertilization (IVF) lab routine due to the reported significantly higher survival and intact survival rates [1–3], and due to the subsequent higher implantation, pregnancy  and live birth rates per embryo warmed .
In current practice, supernumerary embryos of sufficient morphological quality resulting from ART are cryopreserved at either cleavage stage (day 2 or day 3) or at blastocyst stage (day 5 or day 6). While culture until blastocyst stage might select against less viable embryos leading to increased pregnancy rate per transfer, freezing at blastocyst stage decreases significantly the number of embryos frozen per cycle, reducing significantly the cumulative pregnancy rate when compared with freezing at cleavage stage . At the moment, there is not sufficient evidence to choose freezing embryos at blastocyst stage rather than on cleavage stage. For that reason we focused our study on day 3 cleavage stage embryos.
Characteristics that are routinely used to evaluate quality of cleavage stage embryos after thawing include the number of blastomeres survived and the presence of mitosis resumption after overnight culture. The relation between these characteristics and implantation potential has been the focus of several studies, mainly performed with slow frozen embryos [6–9]. In embryos slow frozen/thawed on day 3, implantation rate (IR) has been reported to decrease significantly when the number of blastomeres survived is lower than 25 % , or when mitosis is not resumed during 20–24 h overnight culture after thawing [8–10]. However, IR is not affected in 7–8 cell slow frozen/thawed embryos when 2 or less cells are damaged , or in vitrified/warmed day 3 embryos with up to 2 cells damaged, compared to embryos with no cells damaged, as long as the embryo continues to cleave after overnight culture .
In the Alpha consensus paper on key performance indicators related to cryopreservation, it was reported that number of cells, embryo symmetry and fragmentation were similar before vitrification and after warming, but no data were reported on the effect of embryo symmetry and fragmentation after vitrification/warming on implantation rate . During fresh embryo culture, it is known that cleavage stage embryos with asymmetrical blastomeres and/or with fragmentation >10 % are considered to have an impaired implantation potential compared to embryos with symmetrical blastomeres and/or with <10 % fragmentation, . The degree of fragmentation during fresh culture has been related to the incidence of aneuploidies [13, 14] and the asymmetric embryo cleavage is associated with a lower pregnancy rate and higher degree of chromosomal aberration [13, 15, 16]. For that reason, and in order to improve the cost/effectiveness of a fresh IVF cycle and subsequent frozen-thawed embryo transfer (FET) cycles, embryo selection is done before cryopreservation based on morphological characteristics. In the Alpha consensus meeting on cryopreservation, it has been recommended that only optimal cleavage stage embryos should be cryopreserved , i.e. 4-cell stage embryos at 44 ± 1 h post insemination, 8-cell stage embryos at 68 ± 1 h post insemination with <10 % fragmentation, stage-specific cell size and no multinucleation. However, in practice, most authors report less strict criteria for embryo selection before cryopreservation [4, 10, 17–27]. At present, it is not clear if the stricter embryo selection criteria for cryopreservation, reported in the Alpha consensus, actually result in higher IR compared to clinical practices where less strict criteria are applied. This is clinically important, since it is not in the interest of the patient to discard embryos with sufficient quality and sufficient survival and implantation potential after thawing/warming, even if they do not meet the Alpha consensus criteria. In order to resolve this issue, it is important to determine to which extent implantation rate per embryo after vitrification/warming is determined by not only morphological (i.e. number of blastomeres, symmetry and fragmentation, intact survival and mitosis resumption) but also morphometric (i.e., total cell volume, symmetry, fragmentation and volume change) characteristics. Indeed, in previous research, we demonstrated in fresh cycles that total embryo volume is associated with pregnancy in a quadratic nature, i.e. both lower and higher volumes were associated with a lower pregnancy rate, based on a computer assisted embryo scoring system calculating both embryo symmetry and fragmentation from individual blastomere volume and total cell volume (TCV) of the embryo . Moreover, the morphometric analysis has the advantages of reducing the intra- and inter-observer variability, reducing subjectivity and allowing evaluation without time restriction .
In order to find new morphometric embryo parameters that can improve the selection criteria for both vitrification and embryo transfer after warming, we tested the hypothesis that the implantation rate of embryos warmed after vitrification on day 3 is influenced by morphometric characteristics (i.e., total cell volume, symmetry, fragmentation and volume change). In addition we also studied IR related to standard embryo morphological characteristics after warming (i.e. number of blastomeres, symmetry and fragmentation, intact survival and mitosis resumption).
Patient and embryo selection
In this retrospective analysis, the study population was selected from embryos warmed between September 2011 and December 2014 in our center. All survived and transferred embryos from patients with female age < 40 years at oocyte retrieval (OR) were included. Patients with female age >40 years at OR, cycles with preimplantation genetic diagnosis and donated gametes were excluded. The study population consisted, in total, of 986 embryos from 424 patients transferred in 671 FET cycles.
All procedures were performed according to the Helsinki declaration on Human Experimentation. The study was approved by the Commission for Medical Ethics of the university Hospital Leuven (approval reference number S55685) and registered as a clinical trial (NCT02639715).
Fresh cycle, embryo evaluation and cryopreservation by vitrification
In fresh cycles, ovarian stimulation and luteal supplementation were performed as described previously . Oocyte aspiration, insemination and embryo culture were carried out as foresaid . Embryos were evaluated for fertilization on day 1 after OR (16–20 h after insemination/injection), and for quality on day 2 (41–44 h after insemination/injection) and day 3 (66–71 h after insemination/injection). Embryo quality was assessed using the manual scoring system of the Leuven University Fertility Centre which is based on the visual evaluation of the number and size of blastomeres and the degree of fragmentation . Briefly, embryos were assigned to one of the 4 categories according to their degree of fragmentation (0 %, 1–10 %, 10–25 %, >25 %) and to one of the 3 categories according to their degree of blastomere symmetry (>75 %, 75–50 %, <50 %). On day 3 one or two embryos were chosen for transfer –according to the Belgian law – based on the manual scoring system.
Supernumerary embryos were cryopreserved on day 3 after OR if the quality was sufficient. This was defined as embryos in ≥ 6 cell stage on day 3 with ≤ 25 % fragmentation and with symmetry > 50 % on day 3 . Embryos were vitrified using Vit Kit®–Freeze (Irvine Scientific, Newtownmountkennedy, Ireland), loaded into CBS-VIT-High Security straws (CBS, Cryo Bio System, L’Aigle, France) and plunged directly into liquid nitrogen. Embryos were further stored in vapour phase nitrogen container.
Warmed embryos were transferred in natural cycles, stimulated cycles (gonadotrophin or clomiphene citrate) or hormonal replacement cycles as described previously . Straws were warmed following manufacturer’s protocol Vit Kit®-Thaw (Irvine Scientific, Newtownmountkennedy, Ireland) until the number of survived embryos was equal to the number of requested embryos for transfer. A maximum of two embryos were replaced as determined by Belgian law .
After warming, embryos were cultured overnight in GM501 medium (Gynemed, Lensahn, Germany) under mineral oil (Gynemed) at 37 °C, pH 7.25–7.35 in a standard incubator (Sanyo MCO-20AIC, Osaka, Japan).
Manual morphological evaluation after warming
Embryo quality was evaluated in all embryos (n = 986) immediately after warming (5–30 min) to evaluate survival and after overnight culture (20–24 h) to evaluate mitosis resumption. Fragmentation and symmetry were manually scored at both times. Embryos were considered survived if they had ≥ 50 % of cells intact immediately after warming and were considered intact survived if 100 % of the blastomeres had survived. Mitosis resumption after overnight culture was defined as an increase of the number of blastomeres or compaction of the survived embryos after overnight culture. No selection was performed in the warmed embryos for transfer. Thus, all embryos with or without mitosis resumption were transferred.
Computer based morphometric analysis of embryos before and after warming and overnight culture
On a subset of embryos warmed between January and December 2014 (n = 654) multilevel images composed of 40 different focal planes were taken on day 1 after OR, at freezing (day 3), after warming and after overnight culture. These pictures were only available in this subset, since multilevel imaging was not performed in our laboratory before January 2014 or after December 2014. The available images were analysed retrospectively using Fertimorph Software (CellCura Software Solutions Copenhagen, Denmark). Using this software the total cell volume (TCV) of the embryo was calculated based on the manual drawing of the two diameters of every blastomere . Criteria were pre-established to differentiate a blastomere and a fragment based on the findings by Hnida et al.  and Johanson et al. . A blastomere should be ≥40 μm on day 3 when the embryo had ≤8 blastomeres. When the embryo had >8 blastomeres the minimum diameter for a blastomere was established as 35 μm. Missing values were present when no measurements could be performed, i.e. if the embryo was compacted or if the image was not stored.
Subsequently, the TCV of the embryo was used to calculate four parameters: fragmentation on day of freezing, blastomere symmetry, and volume change. To calculate the fragmentation (%) it was assumed that TCV stays constant through the embryo development. It was calculated as shown in Equation 1. Fragmentation results in negative value when the TCVday3 > TCVday1. In that case fragmentation was considered 0 %. If fragmentation resulted in ≤ -20 % the embryos were excluded from the analysis.
Symmetry (%) was calculated as shown in Equation 2. More symmetrical blastomeres result in a higher %.
Since cryopreservation media cause exchange of water and medium through the embryo membrane, the difference in volume between day 3 and day of warming and the difference in volume between the day of warming and after overnight culture were also of interest.
Fragmentation using morphometrics was only measured before embryo vitrification since the measurement assumes that the total embryo volume does not vary during the days of development. After vitrifying and warming water is substituted by cryoprotectant and vice versa, which causes successive phases of shrinkage and re-expansion  impeding to assume that the embryo volume is constant after warming. Instead of fragmentation, the volume change after warming and after overnight culture was calculated using morphometrics.
Study design and statistical analysis
Morphological characteristics of vitrified/warmed embryos
After overnight culture
The proportion of embryos observed in the different categories of number of blastomeres, symmetry and fragmentation are summarized in Table 1.
Morphometric characteristics of vitrified/warmed embryos measured at each evaluation moment
At freezing (Day 3)
After overnight culture
Total cell volume (μm3)
Mean ± SD
827,074 ± 85,461
724,173 ± 88,648
693,552 ± 117,108
626,881 ± 122,672
Mean ± SD
12.9 ± 9.0
Volume change (%)••
Mean ± SD
−4.0 ± 14.5
−6.9 ± 11.3
Blastomere symmetry (%)•••
Mean ± SD
73.7 ± 8.5
73.4 ± 9.3
68.3 ± 8.8
From a descriptive point of view, morphometrics revealed a decrease in TCV over time, both after warming and after overnight culture (Table 2). Specifically, embryos lost in average 4 % of their volume after warming, when compared to their volume before vitrification, and they lost an additional 7 % after overnight culture, when compared to their volume after warming. Moreover, we observed no change in blastomere symmetry after warming i.e., an average of 74 % symmetry was observed both before and after warming. After overnight culture, symmetry slightly decreased to 68 %.
Implantation rate according to embryo morphometric characteristics
At freezing (Day 3)
After overnight culture
12.0 % (6/50)
13.0 % (17/131)
8.0 % (18/224)
14.6 % (7/48)
Volume change (only intact embryos)
11.6 % (20/172)
5.1 % (2/39)
11.1 % (24/216)
11.3 % (12/106)
13.7 % (29/211)
13.5 % (30/222)
2.7 % (1/37)
8.9 % (25/281)
10.2 % (27/266)
9.7 % (14/145)
This study aimed to find new parameters to improve the selection criteria both for vitrifying and for transferring day 3 embryos after warming. For this purpose, we investigated the IR of day 3 vitrified/warmed embryos in relation to standard morphological characteristics (intact survival, mitosis resumption, number of blastomeres, symmetry and fragmentation) as well as to morphometric characteristics (fragmentation, symmetry and volume change), both after warming and after overnight culture. Due to the fact that embryo scoring has an important subjective component we included, for the first time, in day 3 embryos after warming, a computer based morphometric analysis of symmetry, fragmentation and volume change, due to their reduced subjectivity and intra-observer variability . In contrast with our hypothesis, implantation rate after warming was not related to morphometric evaluation of symmetry, fragmentation and volume change, and was only determined by morphological characteristics. We found that 80 % of the embryos survived intact after vitrification/warming and 67 % of embryos contained >9 cells after overnight culture. In addition, they did not experience significant changes in symmetry, fragmentation and volume. We observed that when mitosis resumption was present, intact embryos had a significantly higher IR than non-intact embryos. Remarkably, embryos with <6 cells after warming or with ≤6 cells after overnight culture had extremely low IR (<1 %).
For slow frozen/thawed embryos, the relation of intact survival and mitosis resumption with IR has been widely described [6–9]. However, to the best of our knowledge, at the moment only one study shows a relation of intact survival and mitosis resumption with IR in vitrified embryos . In slow frozen embryos, the presence of mitosis resumption improved IR [8–10] while IR decreased with the number of cells lost . More concretely, the loss of >25 % of the embryo after thawing was detrimental for IR  and specifically for 7- and 8-cell embryos the loss of >2 cells reduced significantly IR . In the case of vitrified embryos, Van Landuyt et al.  found no effect on IR of 294 SET when embryos lost up to 2 cells and as long as the embryo continued to cleave after overnight culture. Our results in 830 vitrified embryos do not support these findings, since we observed that non-intact embryos had lower IR compared to intact embryos even if mitosis resumption was present (7.5 vs. 15.5 %, p = 0.03). To the best of our knowledge, we are the first group to report that the number of cells damaged had no effect on IR if the embryo had compacted after overnight culture. In addition, if mitosis resumption was not present, intact and non-intact embryos reached the same IR. Besides intact survival and mitosis resumption, the specific number of blastomeres after overnight culture is a characteristic that has been described to influence the IR of survived day 2 embryos after slow freezing [36, 37]. To the best of our knowledge, our study describes for the first time the IR in relation to the specific number of blastomeres in vitrified/warmed embryos. According to our results, the number of blastomeres after overnight culture has an effect on implantation regardless of cell stage after warming (p = 0.01). The majority of the warmed and survived embryos had 8 cells after warming (32.0 %) and >9 cells after overnight culture (66.9 %), which is comparable with results reported by Van Landuyt et al. . We observed that embryos with 8 cells after warming or >9 cells after overnight culture –including compacted embryos– reached the highest IR (>15 %). The IR of embryos in other cell stages was at least 5 % lower (Fig. 4). Interestingly, embryos with <6 cells after warming or with ≤6 cells after overnight culture had an IR <1 %.
In the cryopreservation of cleavage stage embryos, intact survival, mitosis resumption and IR are the key performance indicators defined by the Alpha Scientists in Reproductive Medicine . This group of experts defined competence level and benchmark for each of these parameters. Our intact survival rate (80 %) is between the competence level (70 %) and the benchmark (85 %) and is in line with previous publications, which obtained 75–83 % intact survival rate after warming [4, 10, 19–21, 24, 26, 38]. In the case of the mitosis resumption rate, the Alpha Scientists in Reproductive Medicine  defined this benchmark only for intact embryos, which should be a maximum of 10 % less than the good quality fresh embryos at the same stage. However, since in our lab the embryos are transferred on day 3, no data in fresh embryos were available to compare the cleavage to day 4 of vitrified/warmed embryos. Nevertheless, the literature describes similar mitosis resumption rates (78–88 %) to our results (85 %) in embryos survived after vitrification [4, 10, 26]. Hence, other studies also showed that mitosis resumption is higher in intact than in non-intact embryos (93 vs. 70 %,  and 81 vs. 69 % ) supporting our results (86 vs. 70 %; p = 0.001). Furthermore, we observed that the % of embryos in the stage of morula or early blastocyst increased from 0.4 % after warming to 31 % after overnight culture, indicating a good overnight development.
The overall IR per transferred embryo in our study is 16.9 % (167/986), which in line with previous studies (16–21 % [4, 26]). Since our aim was to relate individual embryo morphological characteristics with IR we needed to calculate the IR per embryo transferred using only embryos transferred in SET or DET with either 0 or 100 % implantation. Thus, IR was known for a subset of 830/986 embryo and resulted in 12.4 % (103/830). The decrease of IR per embryo transferred in respect to the overall IR is explained by the exclusion of DETs with implantation of only one embryo. The benchmark described for IR of cleavage stage embryos after vitrification in the Alpha consensus meeting on cryopreservation  is the same as for mitosis resumption, i.e. maximum of 10 % less than the fresh embryos of the same stage, and such population is not available for comparison in our lab. In summary, our analysis of morphological characteristics is in line with previous studies and can reach the benchmark established by experts.
Despite the fact that morphometric characteristics were not related to IR in day 3 vitrified/warmed embryos, the measurement of these characteristics revealed several new descriptive features regarding fragmentation, blastomere symmetry and volume change that could not be observed otherwise (i.e. with manual evaluation). In respect to fragmentation, an average of 13 % fragmentation was observed before vitrification. Even though embryos selected for cryopreservation can have up to 25 % fragmentation, the majority of embryos presented 1–10 % fragmentation, both after warming and after overnight culture (50 and 36 % respectively). This might indicate a subjective preferential selection of embryos with ≤10 % fragmentation for cryopreservation by the observers. If this is true, the use of morphometrics to select embryos for cryopreservation with <25 % fragmentation would lead to an increase of the number of embryos with >10 % fragmentation cryopreserved. If contrarily, there is no preferential selection by the observers, the lower proportion of embryos with >10 % fragmentation could be explained by the association of other suboptimal characteristics to the degree of fragmentation. In other words, embryos with >10 % fragmentation might have as well an insufficient number of blastomeres or % symmetry that do not fulfil the criteria for cryopreservation. Further studies about manual and computer assisted intra- and inter-observer variability in selecting embryo for cryopreservation as well as studies about association of % fragmentation with % symmetry and number of blastomeres would be needed to support these hypothesis. As for blastomere symmetry, when measured using morphometrics, the average symmetry was similar before vitrification and immediately after warming (73.7 vs. 73.4 %) but it experienced a slight decrease of 5 % after overnight culture. The decrease of symmetry after overnight culture could also be seen with the manual evaluation of symmetry (Table 1). In that case, we found higher IR with a higher blastomere symmetry only at the day of warming. Nevertheless, we would rather carefully interpret this result since this relation was not significant when symmetry was evaluated after overnight culture and was not significant when symmetry was evaluated using morphometrics. The only results available regarding degree of symmetry in cryopreserved embryos describe a positive influence of high blastomere symmetry on day 2–3 during fresh culture on implantation after thawing . The putative importance of the blastomere symmetry on day 3 could be explained by the fact that equally sized blastomeres are expected when the embryo has 4, 8 or 16 cells. On day 3, 8-cell stage embryos are the majority, both before vitrification and after warming, while, on day 4 (after overnight culture), the majority of the embryos have >9 cells but very few have 16 cells due to appearance of compaction, thus presenting lower blastomere symmetry. In regard to the volume change, the embryos lost a mean of 4 % of the volume after warming and 7 % of the volume after overnight culture, nevertheless with a high variance (Table 2), indicating that some embryos do experience a volume increase in spite of the vitrification/warming procedure. This variability in volume caused by vitrification has been described in oocytes [11, 39, 40] but has never been quantified or related to clinical outcome. We hypothesized that volume change caused by vitrification might be an indicator of implantation potential. Our results show that although the majority of embryos have a reduced volume after warming compared to before vitrification, some embryos do recover their volume or even increase it. However, embryos with gain or with loss of volume did not have different IR suggesting that the volume change does not influence the embryo developmental and implantation potential. It is important to notice that morphometric parameters cannot be measured in compacted embryos, therefore, the number of missing values in the evaluation of morphometric characteristics after overnight culture need to be interpreted with caution. Nevertheless, the fact that vitrified embryos present a high compaction rate after overnight culture is already a good prognosis indicator, which decreases the importance of morphometric measurements at that stage.
In our opinion, our criteria to select day 3 embryos for cryopreservation using vitrification is strict enough, even though more strict criteria are proposed by the Alpha consensus . While we select supernumerary embryos for freezing if they have ≥ 6 cell stage on day 3, ≤ 25 % fragmentation and >50 % symmetry on day 3, Alpha consensus only recommends freezing embryos that had 4 cells on day 2 and 8 cells on day 3 –excluding all embryos that had a different evolution and cell number–, <10 % fragmentation, stage-specific cell size –giving no specification on the symmetry– and no multinucleation. We observed no effect of blastomere symmetry and fragmentation on implantation rate, which suggests that embryos with reduced IR due to these characteristics were already excluded before cryopreservation. Therefore, excluding vitrified and warmed embryos for transfer based on fragmentation >10 % and blastomere symmetry <25 % would not improve IR, but would in contrast lead to discarding embryos with acceptable implantation potential. The most important parameter to take into account in the evaluation of vitrified day 3 embryos after warming according to our study is the number of blastomeres, since this is an indicator of both intact survival and mitosis resumption. According to the IR observed in respect to the number of blastomeres after warming, we would strongly recommend to (1) preferentially warm the 8 cell embryos, because they have the highest IR and (2) to warm an extra embryo if available when an embryo does not survive intact with <6 cells, due to their extremely low IR, as recommended before . We agree with the recommendation  that, in case of 8-cell embryos, when more than 2 cells are damaged an extra embryo should be thawed and in case of 6- and 7-cell embryos, when more than one cell is damaged another embryo should be thawed.
In conclusion, after warming and survival, IR of vitrified embryos is determined by the number of cells lost, by the occurrence of mitosis resumption, and by the specific number of blastomeres present. This IR was not affected by fragmentation, blastomere symmetry and volume change, most likely because embryo selection for these criteria takes place before vitrification.
ART, assisted reproductive technology; DET, double embryo transfer; FET, frozen-thawed embryo transfer; IR, implantation rate; IR, implantation rate; IVF, in vitro fertilization; OR, oocyte retrieval; SET, single embryo transfer; TCV, total cell volume
The authors thank the medical, paramedical and technical staff of the Leuven University Fertility Center. We thank Professor Ben Van Calster (Faculty of Medicine, University of Leuven) for the advice on the statistics.
Availability of data and materials
The datasets analysed during the current study are available from the corresponding author on reasonable request.
All authors were involved in the design and conception of the study. EFG performed data analysis and writing of the manuscript, interpretation of the data was done by EFG, SD and CS. All authors read the manuscript critically and gave final approval of this manuscript.
The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
T.D. reports grants from Ferring, Merck Serono, personal fees from Astellas, Actavis, Bayer, Roche Diagnostics, Cartagenia, outside the submitted work. Since October 1st 2015, he has been appointed as Vice-President and Head, Global Medical Affairs Fertility with Merck, Darmstadt, Germany. The work published here was done entirely before that appointment, in his role as Clinical and Academic Head, Division of Reproductive Medicine, Leuven University Hospitals. He continues his academic appointment at KU Leuven as Professor in Reproductive Medicine.
Consent for publication
Ethics approval and consent to participate
The study was approved by the Commission for Medical Ethics of the university Hospital Leuven (approval reference number S55685) and registered as a clinical trial (NCT02639715). All procedures were performed according to the Helsinki declaration on Human Experimentation.
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- Loutradi KE, Kolibianakis EM, Venetis CA, Papanikolaou EG, Pados G, Bontis I, et al. Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil Steril. 2008;90:186–93.View ArticlePubMedGoogle Scholar
- Kolibianakis EM, Venetis CA, Tarlatzis BC. Cryopreservation of human embryos by vitrification or slow freezing: which one is better? Curr Opin Obstet Gynecol. 2009;21:270–4.View ArticlePubMedGoogle Scholar
- AbdelHafez FF, Desai N, Abou-Setta AM, Falcone T, Goldfarb J. Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis. Reprod Biomed Online. 2010;20:209–22.View ArticlePubMedGoogle Scholar
- Debrock S, Peeraer K, Fernandez Gallardo E, De Neubourg D, Spiessens C, D’Hooghe TM. Vitrification of cleavage stage day 3 embryos results in higher live birth rates than conventional slow freezing: a RCT. Hum Reprod. 2015;30:1820–30.View ArticlePubMedGoogle Scholar
- Glujovsky D, Blake D, Farquhar C, Bardach A. Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology. Cochrane Db Syst Rev. 2012;7. doi:https://doi.org/10.1002/14651858.CD002118.pub4. http://onlinelibrary.wiley.com/doi/10.1002/14651858.CD002118.pub4/full.
- Tang R, Catt J, Howlett D. Towards defining parameters for a successful single embryo transfer in frozen cycles. Hum Reprod. 2006;21:1179–83.View ArticlePubMedGoogle Scholar
- Zheng X, Liu P, Chen G, Qiao J, Wu Y, Fan M. Viability of frozen-thawed human embryos with one-two blastomeres lysis. J Assist Reprod Genet. 2008;25:281–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Sole M, Santalo J, Rodriguez I, Boada M, Coroleu B, Barri PN, et al. Correlation between embryological factors and pregnancy rate: development of an embryo score in a cryopreservation programme. J Assist Reprod Genet. 2011;28:129–36.View ArticlePubMedGoogle Scholar
- Joshi BV, Banker MR, Patel PM, Shah PB. Transfer of human frozen-thawed embryos with further cleavage during culture increases pregnancy rates. J Hum Reprod Sci. 2010;3:76–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Van Landuyt L, Van de Velde H, De Vos A, Haentjens P, Blockeel C, Tournaye H, et al. Influence of cell loss after vitrification or slow-freezing on further in vitro development and implantation of human Day 3 embryos. Hum Reprod. 2013;28:2943–9.View ArticlePubMedGoogle Scholar
- Alpha Scientists In Reproductive Medicine. The Alpha consensus meeting on cryopreservation key performance indicators and benchmarks: proceedings of an expert meeting. Reprod Biomed Online. 2012;25:146–67.View ArticleGoogle Scholar
- Alpha Scientists in Reproductive Medicine and Eshre Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26:1270–83.View ArticleGoogle Scholar
- Ziebe S, Lundin K, Loft A, Bergh C, Nyboe Andersen A, Selleskog U, et al. FISH analysis for chromosomes 13, 16, 18, 21, 22, X and Y in all blastomeres of IVF pre-embryos from 144 randomly selected donated human oocytes and impact on pre-embryo morphology. Hum Reprod. 2003;18:2575–81.View ArticlePubMedGoogle Scholar
- Munne S. Chromosome abnormalities and their relationship to morphology and development of human embryos. Reprod Biomed Online. 2006;12:234–53.View ArticlePubMedGoogle Scholar
- Giorgetti C, Terriou P, Auquier P, Hans E, Spach JL, Salzmann J, et al. Embryo score to predict implantation after in-vitro fertilization: based on 957 single embryo transfers. Hum Reprod. 1995;10:2427–31.View ArticlePubMedGoogle Scholar
- Hardarson T, Hanson C, Sjogren A, Lundin K. Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod. 2001;16:313–8.View ArticlePubMedGoogle Scholar
- Desai N, Blackmon H, Szeptycki J, Goldfarb J. Cryoloop vitrification of human day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live births. Reprod Biomed Online. 2007;14:208–13.View ArticlePubMedGoogle Scholar
- El-Danasouri I, Selman H. Successful pregnancies and deliveries after a simple vitrification protocol for day 3 human embryos. Fertil Steril. 2001;76:400–2.View ArticlePubMedGoogle Scholar
- Li Y, Chen ZJ, Yang HJ, Zhong WX, Ma SY, Li M. Comparison of vitrification and slow-freezing of human day 3 cleavage stage embryos: post-vitrification development and pregnancy outcomes. Zhonghua Fu Chan Ke Za Zhi. 2007;42:753–5.PubMedGoogle Scholar
- Balaban B, Urman B, Ata B, Isiklar A, Larman MG, Hamilton R, et al. A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation. Hum Reprod. 2008;23:1976–82.View ArticlePubMedGoogle Scholar
- Wilding MG, Capobianco C, Montanaro N, Kabili G, Di Matteo L, Fusco E, et al. Human cleavage-stage embryo vitrification is comparable to slow-rate cryopreservation in cycles of assisted reproduction. J Assist Reprod Genet. 2010;27:549–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Cercas R, Villas C, Pons I, Brana C, Fernandez-Shaw S. Vitrification can modify embryo cleavage stage after warming. Should we change endometrial preparation? J Assist Reprod Genet. 2012;29:1363–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Cobo A, de los Santos MJ, Castello D, Gamiz P, Campos P, Remohi J. Outcomes of vitrified early cleavage-stage and blastocyst-stage embryos in a cryopreservation program: evaluation of 3,150 warming cycles. Fertil Steril. 2012;98:1138–46 e1.View ArticlePubMedGoogle Scholar
- Shi W, Zhang S, Zhao W, Xia X, Wang M, Wang H, et al. Factors related to clinical pregnancy after vitrified-warmed embryo transfer: a retrospective and multivariate logistic regression analysis of 2313 transfer cycles. Hum Reprod. 2013;28:1768–75.View ArticlePubMedGoogle Scholar
- Xue Y, Tong X, Jiang L, Zhu H, Yang L, Zhang S. Effect of vitrification versus slow freezing of human day 3 embryos on beta-hCG levels. J Assist Reprod Genet. 2014;31:1037–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Fasano G, Fontenelle N, Vannin AS, Biramane J, Devreker F, Englert Y, et al. A randomized controlled trial comparing two vitrification methods versus slow-freezing for cryopreservation of human cleavage stage embryos. J Assist Reprod Genet. 2014;31:241–7.View ArticlePubMedGoogle Scholar
- Chi F, Luo C, Yin P, Hong L, Ruan J, Huang M, et al. Vitrification of day 3 cleavage-stage embryos yields better clinical outcome in comparison with vitrification of day 2 cleavage-stage embryos. Zygote. 2015;23:169–76.View ArticlePubMedGoogle Scholar
- Paternot G, Debrock S, De Neubourg D, D’Hooghe TM, Spiessens C. Semi-automated morphometric analysis of human embryos can reveal correlations between total embryo volume and clinical pregnancy. Hum Reprod. 2013;28:627–33.View ArticlePubMedGoogle Scholar
- Paternot G, Debrock S, D’Hooghe T, Spiessens C. Computer-assisted embryo selection: a benefit in the evaluation of embryo quality? Reprod Biomed Online. 2011;23:347–54.View ArticlePubMedGoogle Scholar
- Debrock S, Melotte C, Spiessens C, Peeraer K, Vanneste E, Meeuwis L, et al. Preimplantation genetic screening for aneuploidy of embryos after in vitro fertilization in women aged at least 35 years: a prospective randomized trial. Fertil Steril. 2010;93:364–73.View ArticlePubMedGoogle Scholar
- De Neubourg D, Bogaerts K, Wyns C, Albert A, Camus M, Candeur M, et al. The history of Belgian assisted reproduction technology cycle registration and control: a case study in reducing the incidence of multiple pregnancy. Hum Reprod. 2013;28:2709–19.View ArticlePubMedGoogle Scholar
- Debrock S, Peeraer K, Spiessens C, Willemen D, De Loecker P, D’Hooghe TM. The effect of modified quarter laser-assisted zona thinning on the implantation rate per embryo in frozen/vitrified-thawed/warmed embryo transfer cycles: a prospective randomized controlled trial. Hum Reprod. 2011;26:1997–2007.View ArticlePubMedGoogle Scholar
- Koninklijk Besluit tot wijziging van het KB van 25 april 2002 betreffende de vaststelling en de vereffening van het budget van financiële middelen voor de ziekenhuizen, art II, bijlage 4.15. Modaliteiten voor de regeling inzake medisch geassisteerde voortplanting. (June 2003).Google Scholar
- Hnida C, Agerholm I, Ziebe S. Traditional detection versus computer-controlled multilevel analysis of nuclear structures from donated human embryos. Hum Reprod. 2005;20:665–71.View ArticlePubMedGoogle Scholar
- Johansson M, Hardarson T, Lundin K. There is a cutoff limit in diameter between a blastomere and a small anucleate fragment. J Assist Reprod Genet. 2003;20:309–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Guerif F, Bidault R, Cadoret V, Couet ML, Lansac J, Royere D. Parameters guiding selection of best embryos for transfer after cryopreservation: a reappraisal. Hum Reprod. 2002;17:1321–6.View ArticlePubMedGoogle Scholar
- Zhang S, Lu C, Lin G, Gong F, Lu G. The number of blastomeres in post-thawing embryos affects the rates of pregnancy and delivery in freeze-embryo-transfer cycles. J Assist Reprod Genet. 2009;26:569–73.View ArticlePubMedPubMed CentralGoogle Scholar
- Rama Raju GA, Haranath GB, Krishna KM, Prakash GJ, Madan K. Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates. Reprod Biomed Online. 2005;11:434–7.View ArticlePubMedGoogle Scholar
- Van Blerkom J, Davis PW. Cytogenetic, cellular, and developmental consequences of cryopreservation of immature and mature mouse and human oocytes. Microsc Res Tech. 1994;27:165–93.View ArticlePubMedGoogle Scholar
- Smith GD, Silva ESCA. Developmental consequences of cryopreservation of mammalian oocytes and embryos. Reprod Biomed Online. 2004;9:171–8.View ArticlePubMedGoogle Scholar