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Table 2 Morphometric characteristics of vitrified/warmed embryos measured at each evaluation moment

From: Effect of embryo morphology and morphometrics on implantation of vitrified day 3 embryos after warming: a retrospective cohort study

  

Day 1

At freezing (Day 3)

After warming

After overnight culture

Total cell volume (μm3)

n

595a

597b

593c

222d

Mean ± SD

827,074 ± 85,461

724,173 ± 88,648

693,552 ± 117,108

626,881 ± 122,672

Fragmentation• (%)

n

-

547a,b,e

-

-

Mean ± SD

-

12.9 ± 9.0

-

-

Volume change (%)••

n

-

-

560b,c,f

216c,d,g

Mean ± SD

-

-

−4.0 ± 14.5

−6.9 ± 11.3

Blastomere symmetry (%)•••

n

-

597b

593c

222d

Mean ± SD

-

73.7 ± 8.5

73.4 ± 9.3

68.3 ± 8.8

  1. For each characteristic the number of embryos from which the characteristics were measured and the mean value ± standard deviation is shown
  2. All morphometric characteristics were calculated based on the total cell volume (TCV) of the embryo at the particular evaluation moment
  3. •Difference of TCV in percentage between embryo at freezing and same embryo on day 1. This measurement assumes that embryo TCV stays constant through the development. Due to the process of vitrification we cannot assume this, thus fragmentation was not calculated after warming
  4. ••Difference of TCV in percentage between the same embryos at two consecutive evaluation moments
  5. •••Difference of diameter in percentage between the biggest and the smallest blastomere of the same embryo at the same evaluation moment
  6. a59/654 embryos had no picture on Day1
  7. b21/654 embryos had no picture at freezing and 36/654 embryos were compacted at freezing
  8. c41/654 embryos had no picture after warming and 20/654 embryo were compacted after warming
  9. d27/654 embryos had no picture after overnight culture and 405/654 embryos were compacted after overnight culture
  10. e9/59 embryos with no picture on Day 1 had also no picture at freezing and 4/59 embryos with no picture on Day 1 were compacted at freezing. 4/654 embryos had fragmentation ≤ -20 % and were excluded
  11. f3/41 embryos with no picture after warming were compacted before freezing, 6/41 embryos with no picture after warming had no picture at freezing. 15/20 embryos that were compacted after warming were also compacted before freezing
  12. g1/27 embryos with no picture after overnight culture was compacted after warming. 21/27 embryos with no picture after overnight culture had no picture after warming. 14/405 embryos compacted after overnight culture had no picture after warming. 19/405 embryos compacted after overnight culture were compacted after warming
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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