Whole ovary immunohistochemistry for monitoring cell proliferation and ovulatory wound repair in the mouse
© Singavarapu et al; licensee BioMed Central Ltd. 2010
Received: 9 June 2010
Accepted: 16 August 2010
Published: 16 August 2010
Ovarian surface epithelial cells are thought to be a precursor cell type for ovarian carcinoma. It has been proposed that an increased rate of ovarian surface epithelial cell proliferation during ovulatory wound repair contributes to the accumulation of genetic changes and cell transformation. The proliferation of ovarian surface epithelial cells during ovulatory wound repair has been studied primarily using immunohistochemical staining of paraffin-embedded ovary sections. However, such analyses require complex reconstruction from serially-cut ovary sections for the visualization and quantification of the cells on the ovarian surface. In order to directly visualize the proliferation and organization of the ovarian surface epithelial cells, we developed a technique for immunohistochemical staining of whole mouse ovaries. Using this method, we analyzed cell proliferation and morphologic changes in mouse ovarian surface epithelial cells during follicle growth and ovulatory wound repair.
Three-week old FVB/N female mice were superovulated by sequential administration of pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Ten hours after hCG administration, mice were given 5-bromo-2-deoxyuridine (BrdU) and euthanized two hours after BrdU administration for ovary isolation. The levels of incorporated BrdU in the ovarian surface epithelial cells were measured by staining paraffin-embedded ovary sections and whole ovaries with the BrdU antibody. Re-epithelialization of the ovarian surface after ovulatory rupture was visualized by immunohistochemical staining with E-cadherin and Keratin 8 in paraffin-embedded ovary sections and whole ovaries.
We determined that active proliferation of ovarian epithelial surface cells primarily occurs during antral follicle formation and, to a lesser extent, in response to an ovulatory wound. We also demonstrated that ovarian surface epithelial cells exhibit a circular organization around the wound site
Whole ovary immunohistochemistry enables efficient and comprehensive three-dimensional visualization of ovarian surface epithelial cells without the need for laborious reconstruction from immunohistochemically-stained serial ovary sections.
Epidemiologic studies show a direct correlation between the number of ovulatory cycles and the risk of ovarian cancer [1–3], suggesting that ovulation may play a role in ovarian carcinogenesis . It is thought that hormone-induced growth of follicles and/or repair of the ovulatory wound result in rapid proliferation of the ovarian surface epithelial cells, which may increase the frequency and accumulation of spontaneous mutations [5, 6]. Analyses of cell proliferation and morphologic changes in mouse and rat ovarian surface epithelial cells during different stages of ovulation have been done previously by immunohistochemical staining of paraffin-embedded ovary sections with antibodies against BrdU or proliferating cell nuclear antigen (PCNA) [7–9]. Such analyses are useful for the visualization of ovulation-induced events inside the ovary, however, visualization and quantification of the cells on the ovarian surface require complex three-dimensional reconstruction from serially-cut ovary sections. In order to directly visualize and quantify the proliferating cells on the ovarian surface during different stages of ovulation, we adapted the protocol for immunohistochemistry on slides to whole mouse ovaries.
Superovulation in mice
The use of mice was in accordance with the NIH Guide for the Care and Use of Laboratory Animals as well as a protocol approved by the Massachusetts General Hospital Subcommittee on Research Animals (SRAC). The mice were housed with 12 hour light/dark cycle and free access to food and water. Follicle growth and subsequent ovulation in 20 three-week old FVB/N female mice (Charles River Laboratory, Wilmington, MA) were induced by intraperitoneal (i.p.) administration of 5 IU of pregnant mare's serum gonadotropin (PMSG) (Calbiochem, Gibbstown, NJ), followed by i.p. administration of 5 IU of human chorionic gonadotropin (hCG) (Sigma, St. Louis, MO) 46 hours later . The mice were euthanized 12 hours after hCG injection for ovary isolation. Two hours before ovary isolation, 100 mg/kg BrdU (Zymed Laboratories, San Francisco, CA) was administered intraperitoneally into the superovulated mice. Hormone induction typically resulted in 5 to 20 ovulatory sites per ovary. Following bursal removal, the ovaries were isolated, fixed in 10% buffered formalin for 6-12 hours, and randomized for immunohistochemistry on paraffin-embedded sections or whole ovaries.
Immunohistochemistry on whole mouse ovaries was done in a 24-well dish that was shaken on a Nutator at room temperature, unless otherwise specified. Fine forceps were used to transfer the formalin-fixed ovaries into a 24-well dish where the ovaries were first washed with phosphate-buffered saline (PBS) then incubated with 3% hydrogen peroxide. For BrdU detection, the ovaries were incubated with hydrogen chloride (2N HCl) for 1 hour, chilled on ice in PBS for 20 minutes, incubated with 0.1% trypsin at 37°C for 20 minutes, and then re-chilled on ice in PBS for 20 minutes (these steps were omitted for E-cadherin and Keratin 8 detection). The ovaries were then transferred into a glass jar with citrate buffer (pH 6.0) (Vector Laboratories, Burlingame, CA) and microwaved for 3 minutes on high power (boiling) and 8 minutes on low power (simmering). After slow cooling for at least 30 minutes, the ovaries were transferred into a 24-well dish on a Nutator and processed for immunohistochemistry with the following peroxidase immunohistochemistry kits from Vector Laboratories: Vector Mouse on Mouse (M.O.M.) for BrdU, Vectastain ABC Rabbit IgG for E-cadherin, and Vectastain ABC Rat IgG for Keratin 8. The ovaries were incubated with the following primary antibodies at room temperature for 30 minutes: mouse monoclonal BrdU (1:100 dilution, Vector Laboratories); rabbit polyclonal E-cadherin (1:100 dilution, Cell Signaling, Danvers, MA); and rat monoclonal TROMA-1 (Keratin 8) (1:25 dilution, Developmental Studies Hybridoma Bank, Iowa City, IA). Bound antibodies were detected by incubating the ovaries with horseradish peroxidase-labeled secondary antibodies (Vector Laboratories) at room temperature for 60 minutes. After color development, the ovaries were washed with PBS. The paraffin-embedded ovary sections were processed for immunohistochemistry in the same manner as the whole ovaries except that the initial steps included deparaffinization and rehydration and the final steps included counterstaining with hematoxylin, dehydration, and mounting with Permount (Fisher Scientific, Pittsburgh, PA). The reproducibility of whole ovary immunohistochemistry was comparable to immunohistochemistry on paraffin-embedded sections; both techniques showed minor day-to-day variations in the intensity of staining. Ovary sections and whole ovaries were visualized using an Olympus BX51 light microscope and an Olympus SZX16 stereomicroscope, respectively (Olympus, Center Valley, PA). The images were captured using a DC-330 3CCD color camera (DAGE-MTI, Michigan City, IN) and adjusted for brightness and contrast using Adobe Photoshop.
Data were analyzed and presented as dot plots using GraphPad Prism software (GraphPad Software, La Jolla, CA). The statistical significance of differences in the numbers of BrdU positive cells between antral follicles and ovulatory wounds was determined with the Student's t test. A value of P < 0.05 was considered to be statistically significant.
Detection of epithelial cell proliferation in the ovaries of superovulated mice
Oocyte release during ovulation results in a wound that needs to be repaired by the proliferation of ovarian surface epithelial cells. To better understand and visualize the proliferation of ovarian surface epithelial cells during follicle growth and ovulatory wound repair, we modified the conventional immunohistochemistry protocol for paraffin sections by adapting it to whole ovaries and compared the immunohistochemistry of ovary sections to the whole ovary method by BrdU analysis. For this purpose, 20 three week-old female mice were superovulated by sequential intraperitoneal administration of PMSG and hCG , and pulsed for 2 hours with BrdU. The ovaries were isolated 12 hours after hCG administration because this is the time when ovulation is expected to occur . Hormone induction typically resulted in 5 to 20 ovulatory sites per ovary. Twenty ovaries were processed as paraffin sections and 20 were used for whole ovary immunohistochemistry.
Re-epithelialization of the ovulatory wound
Most studies on the proliferation of ovarian surface epithelial cells during ovulatory follicle growth and postovulatory wound repair rely on the immunohistochemistry of paraffin sections of the ovaries, which lack information on the spatial distribution of proliferating ovarian surface epithelial cells. To better visualize the proliferation of the ovarian surface epithelial cells during follicle growth and ovulatory wound repair, we applied a modified immunohistochemistry protocol to whole ovaries and compared our method to conventional immunohistochemistry on paraffin sections. Using ovary sections and whole ovaries, we determined that ovarian surface cell proliferation is more active during antral follicle growth than during ovulatory wound repair. Our results are consistent with previous reports in which paraffin-embedded sections of hormone-stimulated mouse ovaries and unstimulated rat ovaries were used to determine the rate of cell proliferation in discrete anatomical regions of the ovary [7–9]. One disadvantage of whole ovary immunohistochemistry in comparison to conventional immunohistochemistry on the ovary sections was in the inability to visualize BrdU negative cells on the ovarian surface. However, it is likely that the use of hematoxylin as a contrasting agent in whole ovaries would allow for the visualization of BrdU negative cells in a similar manner as in conventional immunohistochemistry on ovary sections.
Whole ovary immunohistochemistry was particularly useful for the visualization of epithelial cell organization during ovulatory wound repair. We determined that epithelial cells are organized in a circular pattern around the healing ovulatory wound. Moreover, we detected distinct patches of cells that express different levels of E-cadherin. It is presently unclear why the cells that express different levels of E-cadherin are organized into patches and whether these patches play a specific role in ovulatory wound repair.
Although whole mount immunohistochemistry is commonly used with embryo or organ specimens in Drosophila and Xenopus, its use in murine research has been limited to the study of embryogenesis [11–13] and brain development [14–16]. The advantage of using whole ovary immunohistochemistry for the quantification of ovarian surface epithelial cell proliferation and the visualization of ovarian surface re-epithelialization after follicular rupture is the ability to directly visualize the entire surface of the ovary, without the need to reconstruct the surface from serial sections. This technique could be easily adapted for whole ovary immunohistochemistry for other species, including human.
We thank Kristy Daniels for assistance in the preparation of the manuscript, Kader Yagiz from the Gene Therapeutics Research Institute for generating the dot plots, and Beth Karlan and members of the Women's Cancer Research Institute for their support. This work was funded by the Marsha Rivkin Center for Ovarian Cancer Research, the American Cancer Society Research Scholar Grant in Basic, Preclinical, Clinical and Epidemiology Research and the DOD W81XWH-04-1-0485 grant.
- Bernal A, Mendez-Moran L, Fajardo-Gutierrez A, Gonzalez-Lira G, Escudero P, Ortiz H: Univariate and multivariate analysis of risk factors for ovarian cancer: case-control study, Mexico City. Arch Med Res. 1995, 26: 245-249.PubMedGoogle Scholar
- Perez RP, Godwin AK, Hamilton TC, Ozols RF: Ovarian cancer biology. Semin Oncol. 1991, 18: 186-204.PubMedGoogle Scholar
- Purdie DM, Bain CJ, Siskind V, Webb PM, Green AC: Ovulation and risk of epithelial ovarian cancer. Int J Cancer. 2003, 104: 228-232. 10.1002/ijc.10927.View ArticlePubMedGoogle Scholar
- Auersperg N, Edelson MI, Mok SC, Johnson SW, Hamilton TC: The biology of ovarian cancer. Semin Oncol. 1998, 25: 281-304.PubMedGoogle Scholar
- Murdoch WJ, Townsend RS, McDonnel AC: Ovulation-induced DNA damage in ovarian surface epithelial cells of ewes: prospective regulatory mechanisms of repair/survival and apoptosis. Biol Reprod. 2001, 65: 1417-1424. 10.1095/biolreprod65.5.1417.View ArticlePubMedGoogle Scholar
- Murdoch WJ, McDonnel AC: Roles of the ovarian surface epithelium in ovulation and carcinogenesis. Reproduction. 2002, 123: 743-750. 10.1530/rep.0.1230743.View ArticlePubMedGoogle Scholar
- Gaytan M, Sanchez MA, Morales C, Bellido C, Millan Y, Martin de Las Mulas J, Sanchez-Criado JE, Gaytan F: Cyclic changes of the ovarian surface epithelium in the rat. Reproduction. 2005, 129: 311-321. 10.1530/rep.1.00401.View ArticlePubMedGoogle Scholar
- Burdette JE, Kurley SJ, Kilen SM, Mayo KE, Woodruff TK: Gonadotropin-induced superovulation drives ovarian surface epithelia proliferation in CD1 mice. Endocrinology. 2006, 147: 2338-2345. 10.1210/en.2005-1629.View ArticlePubMedGoogle Scholar
- Tan OL, Fleming JS: Proliferating cell nuclear antigen immunoreactivity in the ovarian surface epithelium of mice of varying ages and total lifetime ovulation number following ovulation. Biol Reprod. 2004, 71: 1501-1507. 10.1095/biolreprod.104.030460.View ArticlePubMedGoogle Scholar
- Nagy AM, Gertsenstein K, Vintersten K, Behringer RR: Manipulating the Mouse Embryo. A Laboratory Manual. 2003, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 3Google Scholar
- Mills AA, Mills MJ, Gardiner DM, Bryant SV, Stanbridge EJ: Analysis of the pattern of QM expression during mouse development. Differentiation. 1999, 64: 161-171. 10.1046/j.1432-0436.1999.6430161.x.View ArticlePubMedGoogle Scholar
- Corson LB, Yamanaka Y, Lai KM, Rossant J: Spatial and temporal patterns of ERK signaling during mouse embryogenesis. Development. 2003, 130: 4527-4537. 10.1242/dev.00669.View ArticlePubMedGoogle Scholar
- Irving C: In-situ hybridization and immunohistochemistry in whole embryos. Methods Mol Biol. 2008, 461: 687-695. full_text.View ArticlePubMedGoogle Scholar
- Luque JM, Adams WB, Nicholls JG: Procedures for whole-mount immunohistochemistry and in situ hybridization of immature mammalian CNS. Brain Res Brain Res Protoc. 1998, 2: 165-173. 10.1016/S1385-299X(97)00035-4.View ArticlePubMedGoogle Scholar
- Sillitoe RV, Hawkes R: Whole-mount immunohistochemistry: a high-throughput screen for patterning defects in the mouse cerebellum. J Histochem Cytochem. 2002, 50: 235-244.View ArticlePubMedGoogle Scholar
- Kim SH, Che P, Chung SH, Doorn D, Hoy M, Larouche M, Marzban H, Sarna J, Zahedi S, Hawkes R: Whole-mount immunohistochemistry of the brain. Curr Protoc Neurosci. 2006, Chapter 2Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.