BrdU staining of ovary sections and whole ovaries in superovulated mice. BrdU was administered to the mice 10 hours after hCG injection and the ovaries were isolated 2 hours after BrdU injection. The distribution of cells with incorporated BrdU was determined by staining paraffin-embedded ovary sections (A, C, E) and whole ovaries (B, D, F, G) with anti-BrdU antibody. Arrowheads indicate BrdU positive cells below the ovarian surface epithelial layer. Abbreviations: af, antral follicle; cl, corpus luteum; o, ovulatory wound; or, recent ovulatory wound.