- Open Access
Comparative aspects of trophoblast development and placentation
© Carter and Enders; licensee BioMed Central Ltd. 2004
- Received: 26 December 2003
- Accepted: 05 July 2004
- Published: 05 July 2004
Based on the number of tissues separating maternal from fetal blood, placentas are classified as epitheliochorial, endotheliochorial or hemochorial. We review the occurrence of these placental types in the various orders of eutherian mammals within the framework of the four superorders identified by the techniques of molecular phylogenetics. The superorder Afrotheria diversified in ancient Africa and its living representatives include elephants, sea cows, hyraxes, aardvark, elephant shrews and tenrecs. Xenarthra, comprising armadillos, anteaters and sloths, diversified in South America. All placentas examined from members of these two oldest superorders are either endotheliochorial or hemochorial. The superorder Euarchontoglires includes two sister groups, Glires and Euarchonta. The former comprises rodents and lagomorphs, which typically have hemochorial placentas. The most primitive members of Euarchonta, the tree shrews, have endotheliochorial placentation. Flying lemurs and all higher primates have hemochorial placentas. However, the lemurs and lorises are exceptional among primates in having epitheliochorial placentation. Laurasiatheria, the last superorder to arise, includes several orders with epitheliochorial placentation. These comprise whales, camels, pigs, ruminants, horses and pangolins. In contrast, nearly all carnivores have endotheliochorial placentation, whilst bats have endotheliochorial or hemochorial placentas. Also included in Laurasiatheria are a number of insectivores that have many conserved morphological characters; none of these has epitheliochorial placentation. Consideration of placental type in relation to the findings of molecular phylogenetics suggests that the likely path of evolution in Afrotheria was from endotheliochorial to hemochorial placentation. This is also a likely scenario for Xenarthra and the bats. We argue that a definitive epitheliochorial placenta is a secondary specialization and that it evolved twice, once in the Laurasiatheria and once in the lemurs and lorises.
- Tree Shrew
- Golden Mole
- Rock Hyrax
- Elephant Shrew
- Chorioallantoic Placenta
In marsupials, the separation of embryonic and trophoblastic areas of the blastocyst is less distinct than in eutherians . Most marsupials form only a yolk sac placenta, although bandicoots do have an additional chorioallantoic placenta. Nevertheless, it has been argued that the allantochorion is an ancestral character present in the stem species of marsupials and that the yolk sac became predominant during evolution of the marsupial placenta . Extra-embryonic membranes participate in maternal-fetal exchange in many non-mammalian vertebrates, too, as detailed in several recent publications [3–5]. However, the present review will be restricted to placentation in eutherian mammals.
The interhemal area
Variations in the trophoblast comprising the interhemal area of the chorioallantoic placenta.
Syncytial trophoblast, villous
Armadillo; higher primates
Syncytial trophoblast, labyrinthine
Most hystricomorph rodents (e.g. guinea pig, capybara); sciuromorph rodents (squirrels)
Hyraxes; spiny tenrecs (e.g Echinops); mollosid bats (late gestation); some myomorph rodents (Zapus, Jaculus)
Syncytial (maternal facing) and cellular trophoblast
Lagomorphs; vespertilionid bats
Cellular trophoblast (maternal facing) and two layers of syncytial trophoblast
Most myomorph rodents (e.g. rat, mouse)
Syncytial trophoblast (fenestrated)
Sloths; shrews (insectivores)
Syncytial trophoblast (nonfenestrated)
Carnivores and pinnipeds
Elephants; some sciuromorph rodents (Dipodomys, Microdipodops); some bats (Natalus, Saccopteryx)
Cellular trophoblast only
Lower primates; pangolin; some artiodactyls (e.g. pig)
Cellular trophoblast with trophoblastic girdle cells
Equids (perissodactyls, e.g. horse)
Cellular trophoblast; fusion of binucleate trophoblastic cells with uterine epithelium
Ruminants (artiodactyls, e.g. sheep, cow)
The list in Table 1 by no means exhausts the known number of placental types. Many placentas have specialized areas concerned with histiotrophic nutrition, that is, absorption of secretions from the uterine glands or phagocytosis of cellular debris. A large number have hemophagous regions in which maternal erythrocytes are taken up as a source of iron for the fetus. There are often large areas that seem to be concerned more with protein synthesis than materno-fetal exchange, as they are not penetrated by fetal blood vessels; examples are the spongy zones of rodent and hyrax placentas. Not surprisingly, there are many variations in placental shape, from the diffuse and cotyledonary forms in hoofed mammals through the circumferential ("zonary") placenta of carnivores to the familiar discoid form of the human or mouse placenta.
Clearly each placental type is able to support a successful pregnancy. Nevertheless, students of placental structure have continued to ask why there is so much variability and how the placenta evolved. The debate started with Huxley and Owen in the nineteenth century (reviewed by Pijnenborg and Vercruysse ), continued with Wislocki  and Portmann , and culminated with the publications of Mossman [12, 13] and Luckett . The success of molecular phylogenetics in identifying the relations between the orders of mammals allows us to view placental structure from a new perspective [15, 16]. It appears that all extant eutherian mammals can be classed in four superorders. These have been named Afrotheria, Xenarthra, Euarchontoglires and Laurasiatheria [17, 18]. Accordingly, the approach taken here will be to review placentation in each superorder in turn.
This superorder was proposed following analysis of DNA sequence data. It includes six orders of mammals. Three of these, elephants, sea cows and hyraxes, were already considered to be closely related. Inclusion of the elephant shrews and the aardvark is not controversial. There has, however, been some debate about the sixth order, Tenrecomorpha or Afrosoricida, which comprises the golden moles and tenrecs. This is in part because analysis of morphological characters fails to reveal a single synapomorphy in support of Afrotheria .
All these features are apparent in the placenta and fetal membranes of the manatee , rock hyrax (Figure 2C)  and aardvark [26, 27]. However, whilst the aardvark and possibly the manatee  have endotheliochorial placentation, the rock hyrax placenta is hemomonochorial. The single layer of trophoblast in the interhemal barrier is cellular not syncytial . One could thus envisage how this arrangement could be derived from an endotheliochorial placenta such as that of the African elephant.
Elephant shrews have a discoid placenta. It is hemochorial and the trophoblast in the barrier may be syncytial , although this needs to be re-examined. Elephant shrews do share the large, four-lobed allantoic sac with other afrotherians [30, 31]. Golden moles  and tenrecs  also tend to have discoid, hemochorial placentas. In elephant shrews and tenrecs, there is an additional circumferential area or paraplacenta [31, 33]. We examined the interhemal area of the Madagascar lesser hedgehog tenrec and found that it comprised a single layer of cellular trophoblast similar to the rock hyrax (Figure 2B) . In the otter shrews, the only tenrecs found on mainland Africa, the situation is more complex. There appears to be a circumferential, endotheliochorial placenta (Enders, Carter and Vogel, unpublished data). The giant otter shrew has the four-lobed allantoic sac found in all the other orders of Afrotheria .
Morphological evidence of iron transfer: hemophagous zones.
Base of fetal villi
Dog and cat
Sac wing bat
Base of fetal villi
Lateral area of placental band
Central off labyrinth
Nothing is known about early development in sea cows, but an extensive choriovitelline placenta is formed in all other orders of Afrotheria. Following establishment of the chorioallantoic placenta, however, the yolk sac is very much reduced.
Based on current knowledge of placentation in the extant species, it seems possible that the common ancestor of the Afrotheria had the following features. (1) A circumferential, endotheliochorial placenta as in the elephant, manatee, aardvark and otter shrews. Loss of maternal endothelium would lead to the hemochorial condition seen in rock hyraxes, elephant shrews and some tenrecs. The formation in elephant shrews and tenrecs of a discoid placenta with a more or less extensive paraplacenta would also be a derived state. (2) A prominent hemophagous organ as in otter shrews and tenrecs, retained in elephants, aardvark and manatee, but lost in rock hyraxes and elephant shrews. (3) A four-lobed allantoic sac, retained in otter shrews and all other Afrotheria, but seemingly lost in some members of Tenrecomorpha. (4) A short cord sending four leashes of vessels to the placenta.
The sloth placenta differs in several respects from that of anteaters and armadillos. It is diffuse in early stages but later becomes discoid or even double discoid. The exchange area is labyrinthine, not villous, and the interhemal area is endotheliochorial (Figure 3A). It consists of hypertrophied maternal endothelial cells; a small amount of extracellular material and spindle-shaped cells of presumed maternal origin; a layer of syncytial trophoblast that although thick is fenestrated; and the fetal capillary endothelium. In addition, the fetal connective tissue contains hypertrophied mesenchymal cells similar to those in the armadillo and anteater, but also found in shrews and tree shrews; these cells are characterized by abundant granular endoplasmic reticulum .
In contrast to the afrotherians, xenarthrans have only a rudimentary allantoic sac. However, the armadillos have large inverted yolk sacs that persist well into gestation. Little is known about yolk sac placentation in anteaters. It is doubtful that sloths have a choriovitelline placenta since in late gestation the yolk sac is small and rudimentary.
Since the hemochorial placenta of the armadillo is not preceded by an endotheliochorial condition and the placenta of armadillos and anteaters differs from that of the sloths, it is not clear which might be considered more derived. It is notable, however, that epitheliochorial placentation is not encountered in Xenarthra or Afrotheria, the superorders considered closest to the root of the eutherian tree .
The Superorder Euarchontoglires or Superprimates comprises two sister groups. Glires unites rodents and lagomorphs (rabbits and pikas). The Euarchonta include three orders: primates, flying lemurs and tree shrews
Placentation in Glires
The rodent order is so rich in species that only a small sample of placentas has been described . However, the placentas of rodents and lagomorphs tend to conform to a basic pattern. They are discoid to spherical in shape, the exchange area is labyrinthine and hemochorial, and there is a well developed spongy zone. An inverted or partially inverted yolk sac placenta supports the early development of the embryo and is retained until term.
There are some notable exceptions. The kangaroo rats Dipodomys and Microdipodops are sciuromorph rodents, but in this family (Heteromyidae) the interhemal area is endotheliochorial and contains cellular trophoblast (Figure 4D) . In two families of myomorph rodents, jerboas (Dipodidae) and jumping mice (Zapodidae), there is a reduction in the trophoblast layers and the maternal blood channels are lined by trophoblastic giant cells (Figure 4C) .
In most rodent placentas the labyrinth and spongy zone form distinct layers. In hystricomorph rodents, however, folding gives rise to a lobed placenta. The lobes are the exchange areas and they are separated by interlobular trophoblast that is the equivalent of a spongy zone. In addition, hystricomorphs have a unique structure of unknown function, the subplacenta .
Rodents represent the only order of eutherian mammals for which placentation has been subjected to a strict cladistic analysis. Mess  concluded that the stem pattern was labyrinthine and hemochorial with a single layer of syncytial trophoblast in the exchange area. She did not extend this analysis to include the lagomorphs. It is pertinent to note that, on this analysis, the mouse placenta represents an elaboration of the stem pattern. This has potential implications for discussion of structural and functional homologies between mouse and human placentas [48, 49].
Placentation in Euarchonta
Ever since the seminal work of Hill , the evolution of placentation in primates has posed some interesting questions . Clearly there is a striking difference between the lemurs and lorises (Strepsirhini) and other primates (Haplorhini). The former have epitheliochorial and the latter hemochorial placentation. The recognition that tree shrews were closely related to primates, which receives further support from molecular studies, adds a further layer of complexity, since tree shrews (Scandentia) have endotheliochorial placentas . Finally Hill, who regarded hemochorial placentation as the derived state, opined that it had evolved separately in the tarsiers than in the monkeys and apes.
The colugos or flying lemurs (Dermoptera) have attracted less attention. Molecular phylogeneticists group them with primates and tree shrews, although the relations between the three orders are not well resolved . Their placenta has been little studied. It is discoid and appears to contain labyrinthine and villous areas. The interhemal area is hemodichorial .
Lemurs and lorises present a curious case in that they have diffuse, non-deciduate and epitheliochorial placentas (Figure 5A). The resemblance to placentation in pigs extends to the presence of thickened areas of trophoblast opposite the openings of the uterine glands , which parallel the areolae of the porcine placenta. There is a large allantoic vesicle, which is lobed as in the afrotherians. A temporary yolk sac placenta supports development of the early embryo.
Tarsiers have a discoid, hemochorial placenta. Except that it is labyrinthine rather than villous, the placenta is generally similar to that of monkeys and apes. The allantois is not vesicular, as in lemurs, but forms an almost solid connecting stalk of mesodermal tissue as in higher primates, a point to which Hill  attached particular importance.
There are strong resemblances in the early development of the platyrrhine or New World monkeys, the catarrhine monkeys of the Old World and the anthropoid apes. However, in platyrrhine monkeys, proliferation of the trophoblast continues until much later in gestation and connections persist between the villi, which form a trabecular network, not unlike that of the tarsier placenta. Only at a late stage of fetal development are branched villi found within a more or less continuous intervillous space. In catarrhine monkeys, on the other hand, arborescent chorionic villi are present from a very early stage, as they are in the human placenta. Although macaque and baboon implantation sites develop – sequentially – trophoblastic plate, lacunar and villous stages as do the anthropoid apes, in the former animals trophoblast taps maternal vessels early and the lacunae expand the placenta into the uterine lumen . Macaques also form a secondary placenta on the opposite side of the uterus from the original implantation. In man and anthropoid apes the trophoblast proceeds further into the uterus and the abembryonic as well as the polar trophoblast forms lacunae, resulting in an interstitial placenta. In all of these animals the definitive placenta is discoidal with free and anchoring villi and an intervillous space perfused by maternal blood (Figure 5C).
Based on their diffuse, non-deciduate and epitheliochorial placentation, Hill  argued that the lemurs and lorises were a more primitive form of primate than the tarsiers. This argument went to the very heart of the current debate about parallelism in the evolution of the placenta. Many found it difficult to accept that rodents, insectivores and primates arose from a common stem with a diffuse type of placentation. Among the doubters was Wislocki , who advanced the view that hemochorial placentation was the more primitive type of placentation and that the epitheliochorial placenta of the lemurs was the result of a secondary simplification.
A lucid discussion of the evolution of placentation in primates is given by Luckett . He noted that there is substantial homology in fetal membrane development between taxa with epitheliochorial placentation, but not between those with the endotheliochorial or hemochorial types. He argued that this was indicative of convergent evolution of hemochorial placentation, whereas the epitheliochorial type was part of the primitive eutherian condition.
On the grounds of parsimony, one might argue that the widespread occurrence of hemochorial placentation in Euarchonta reflects their common ancestry and follow Wislocki  in regarding the epitheliochorial placenta of strepsirhine primates as a derived state. Tree shrews have many conserved characters and it is possible that endotheliochorial placentation is one of these rather than being derived from a hemochorial type.
Many types of placentation are found in the Superorder Laurasiatheria. Not surprisingly for an order that has undergone such a high degree of adaptive radiation, the greatest diversity is found among the bats. The insectivores now included in Eulipotyphla also present a variety of placental types. Carnivores and pinnipeds have endotheliochorial placentation. The three remaining orders have epitheliochorial placentas. These are the whales and cloven-hoofed mammals (Cetartiodactyla); horses, tapirs and rhinoceroses (Perissodactyla); and pangolins (Pholidota).
The order of insectivores has undergone steady attrition, the most recent exclusions being the tenrecs and golden moles. The relations between the core insectivores (shrews, moles, hedgehogs and Solenodon) continue to puzzle even the molecular phylogeneticists [57, 58]. Most have discoid labyrinthine placentae. The interhemal area is hemochorial in hedgehogs and Solenodon . The American mole Scalopus has a circumferential placenta that is considered to be epitheliochorial , whereas the European mole Talpa is definitely endotheliochorial . However, Prasad et al.  had only a single midgestation specimen to examine by electron microscopy, and the looping arrangement of the maternal vessels is more similar to that seen in endotheliochorial placentas than that in typical epitheliochorial placentas. In the short-tailed shrew Blarina the interhemal area is endotheliochorial, but the trophoblast component is fenestrated . In the musk shrew Suncus the placenta is endotheliochorial, but there is progressive thinning and fenestration of the trophoblast layer . There are even areas where processes from the two endothelial layers contact one another through the pores in the trophoblast.
All insectivores have a partially or completely inverted yolk sac that is maintained to term. The allantoic sac is large in Talpa, medium sized in the hedgehog, small in Scalopus and small or rudimentary in shrews. This is a group of mammals that would repay close study using modern techniques.
Carnivores and pinnipeds
The terrestrial carnivores and their aquatic relatives the pinnipeds (sea lions, walruses and seals) usually have circumferential, labyrinthine and endotheliochorial placentas, although the hyena develops hemochorial placentation late in pregnancy [66, 67]. Carnivore placentas have hemophagous regions of variable size and location . Mossman  at one time considered the carnivore fetal membranes to be the most primitive type among extant eutherians, because both the yolk sac and allantoic sac are large and persist until term.
Cetartiodactyls, perissodactyls and pangolins
The remaining orders of Laurasiatheria are characterized by epitheliochorial placentation. The placenta may be diffuse with villi distributed over the surface of the chorioallantoc sac, as in whales , Suiformes (pigs, peccaries, and hippopotamus) , camels  and pangolins , or with microcotyledons as in the horse [16, 72]. Most ruminants have cotyledonary placentas with the cotyledons varying in number from 5–8 in deer to 50–175 in bovids . Chevrotains are considered to be the most primitive ruminants and, interestingly, they have diffuse placentas .
The epitheliochorial type of arrangement has as its advantage the safety factor of the isolation of fetal and maternal components. The presence of two complete epithelia should diminish immunological problems as well as the deportation of fetal cells to the maternal organism. The disadvantage is the greater difficulty in passage of materials between organisms, but this is overcome by a variety of mechanisms. Most importantly, indenting of trophoblast and uterine epithelium by capillaries decreases the interhemal distance. There are often, but not always, small absorptive and phagocytic areas, for example the areolae of the pig placenta. In equids, girdle cells of fetal origin invade the uterine epithelium to form endometrial cup cells that secrete equine chorionic gonadotropin [74–76]. However, not all perissodactyls have endometrial cups . In ruminants such as sheep and cow, binucleate trophoblast cells fuse with maternal epithelial cells and this facilitates passage of prolactin to the maternal organism ; this type of placenta is sometimes referred to as synepitheliochorial.
The diffuse type of epitheliochorial placenta usually is associated with an extensive allantoic sac. The cotyledonary type tends to be associated with a long, tubular allantoic cavity, although it is still large. There is a temporary yolk sac placenta early in development, where this has been examined, and a free yolk sac later in gestation. Mossman  noted the resemblance in definitive yolk sac placentation between whales, artiodactyls, perissodactyls and pangolins, but grouped them together with some non-laurasiatherians.
Although placental structure is directly involved in the success of fetal growth, it is only one aspect of the complex interrelationship between reproductive success of individual matings and the overall reproductive success of a given female . Consequently different aspects of the total reproductive process may be more or less important depending on the relationship of a given species to its environment. Nevertheless placental structure has been considered useful as one of the characters to be used in tracing relationships as it is considered conservative . Although physiological data would be useful, unfortunately such data as the increase in fetal weight per weight of placenta per day is available for only a few domestic and laboratory animals, limiting our ability to use such data on a comparative basis .
The evolutionary pressure favoring some type of hemochorial placenta has evidently been great. Hemochorial placentas are found in insectivores, primates, tenrecs, rodents, bats, hyraxes, elephant shrews, anteaters, armadillos, flying lemurs and even hyenas. The large variation in the definitive form of the placenta, the divergent way in which the hemochorial condition is achieved, and the variety of unrelated orders in which it is found might seem to suggest convergent evolution .
Additional clues to placental evolution are provided by the early development of the fetal membranes. Luckett [14, 55] argued that ancestral mammals would have had an amnion formed by folding rather than cavitation and possessed a large allantoic vesicle. A large vascular yolk sac would have been present early in development, forming a choriovitelline placenta, but the yolk sac would have been reduced in later stages . These features are found with endotheliochorial and hemochorial placentas in Afrotheria as well as with epitheliochorial placentas in some Laurasiatheria.
Portmann  argued that the allantoic sac had evolved along two paths. Mammals with epitheliochorial placentation had retained a large allantoic sac as a receptacle for the dilute urine produced by a large and persistent mesonephros. The carnivores had also followed this route. In contrast the allantoic cavity had become reduced or was absent in mammals that had a poorly developed mesonephros, such as rodents, higher primates, bats and xenarthrans. Most of these had a hemochorial placenta that was thought to have assumed the function of the mesonephros as first proposed by Bremer . This hypothesis needs to be tested and refined in the light of current concepts of mammalian phylogenetics and kidney development .
The position argued here resembles that originally adopted by Mossman . He considered that the endotheliochorial and hemochorial conditions were closely related and that the endotheliochorial type was the more primitive. We have shown that the likely path of evolution was from endotheliochorial to hemochorial in the Afrotheria. It certainly occurred with the carnivores and more than likely in the bats and Xenarthra. There may be an epitheliochorial stage during placental ontogeny, but Mossman  argued that a definitive epitheliochorial placenta was a secondary specialization; he regarded the cotyledonary placentas of ruminants as among the most highly specialized. We are inclined to agree. Consideration of placental type in relation to the findings of molecular phylogenetics suggests that epitheliochorial placentation evolved just twice, once in the Laurasiatheria and once in Euarchontoglires.
It is a pleasure to acknowledge Diana Mossman and Paula Holahan for making available material from the Mossman Collection at the University of Wisconsin Zoological Museum. We also wish to thank Graham Burton for access to and assistance with the Boyd Collection at Cambridge University, and Jenny Narraway for access to and help with the Hill and Hubrecht Collections at the Hubrecht Laboratory, Utrecht. The studies were supported in part by The Carlsberg Foundation, Denmark.
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