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Post-weaning diet determines metabolic risk in mice exposed to overnutrition in early life
Reproductive Biology and Endocrinologyvolume 12, Article number: 73 (2014)
Maternal overnutrition during pregnancy is associated with an increased risk of obesity and cardiometabolic disease in the offspring; a phenomenon attributed to ‘developmental programming’. The post-weaning development of obesity may associate with exacerbation of the programmed metabolic phenotype. In mice, we have previously shown that exposure to maternal overnutrition causes increased weight gain in offspring before weaning, but exerts no persistent effects on weight or glucose tolerance in adulthood. In order to determine whether post-weaning exposure to a cafeteria diet might lead to an exacerbation of programmed effects, offspring born and raised by mothers on control (CON) or cafeteria (DIO) diets were transferred onto either CON or DIO diets at weaning.
Post-weaning DIO caused the development of obesity, with hyperglycaemia and hyperinsulinaemia in males; and obesity with hyperinsulinaemia in females and with increased cholesterol levels in both sexes. Exposure to maternal overnutrition during pregnancy and lactation caused only subtle additional effects on offspring phenotype.
These results suggest that post-weaning exposure to a high-fat high-sugar diet has a more profound effect on offspring weight gain and glucose tolerance than exposure to maternal overnutrition. These data emphasise the importance of optimising early life nutrition in offspring of both obese and lean mothers.
Human and animal studies have shown that the environment in early life can increase the risk of later metabolic disease . There is increasing interest in the role of maternal obesity in the ‘programming’ of offspring disease risk  and recent studies have shown that maternal obesity and gestational weight gain are independently associated with offspring cardiometabolic risk and with all-cause mortality [3, 4]. This is of substantial importance given the increasing prevalence of obesity worldwide, including amongst women of childbearing age . In order to understand the mechanisms by which exposure to maternal obesity leads to programming of offspring phenotype, animal models have been developed, many of which recapitulate the findings in human studies, showing effects on offspring adiposity, glucose-insulin homeostasis, blood pressure and appetite [6–8].
Using a mouse model, we recently reported remarkably few effects of maternal overnutrition on body weight and metabolism in the directly exposed (F1) offspring . Despite this, there were effects on birthweight and metabolism in a second generation, suggesting that there were persistent effects in F1 offspring leading to the transmission of effects . Since in humans, postnatal obesity appears to be an important determinant of metabolic disease , and post-weaning exposure to a high-fat diet is associated with amplification of effects in some animal models , we hypothesised that post-weaning exposure to a cafeteria diet would result in amplification of the phenotype in both male and female F1 offspring of overnourished mothers.
Animal studies were conducted as previously reported  under approval by the UK Home Office, under the Animals (Scientific Procedures) Act. The experiments were set up as previously described, using a new cohort of mice. From 5 weeks, female C57BL/6 mice were allowed free access to cafeteria (DIO: 58 kcal% fat, 25.5 kcal% carbohydrate as sucrose) or matched control diets (Con: 10.5 kcal% fat and 73.1 kcal% carbohydrate as corn-starch) (Diets D12331 and D12328, Research Diets, New Brunswick, USA). At 17 weeks, females were time-mated with chow-fed C57BL/6 males (RMI 801002, Special Diets Services, Witham, UK). Females remained on experimental diets through pregnancy and lactation. At postnatal day 1, litters were weighed and reduced to five pups; animals remained with their biological mothers until weaning at 3 weeks. Groups of F1 male and female pups were selected randomly from each litter and weaned onto cafeteria (D12331) or control diets (D12328). This gave four groups of F1 offspring (n = 7-8/group): 1) offspring of Con mothers weaned onto control diet (CON/CON) 2) offspring of Con mothers weaned onto cafeteria diet (CON/DIO) 3) offspring of DIO mothers weaned onto control diet (DIO/CON) and 4) offspring of DIO mothers weaned onto cafeteria diet (DIO/DIO).
Intraperitoneal glucose tolerance testing (GTT) and lipid measurements were performed at 3 and 6 months following a 6-hour fast. A fasting tail blood sample was taken immediately prior to glucose injection after which mice received an intraperitoneal injection of glucose (2 g/kg body weight). Tail blood samples were collected at 15, 30, 60 and 90 minutes, placed on ice, centrifuged at 2.3 × g for 10 minutes at 4°C and the supernatant plasma stored at -20°C. Plasma glucose levels were determined by the hexokinase/glucose-6-phosphate dehydrogenase method (Thermo Fisher Scientific, UK) and plasma insulin by ELISA (Crystal Chem Inc., Downers Grove, IL, USA). We calculated homoeostasis model assessment of insulin resistance (HOMA-IR; fasting plasma glucose [mmol/L] × fasting insulin [mU/L])/22·5). Fasting plasma cholesterol and triglyceride levels were measured by an enzymatic assay following the manufacturer’s instructions (Infinity kits; Thermo Fisher Scientific, UK).
Data are expressed as mean ± SEM. Groups were compared by independent t-tests, Area under Curve, repeated measures ANOVA and two-way ANOVA as appropriate. Data for plasma parameters and organ weights were compared by with pre-weaning and post-weaning diet as the main factors using Statistica (Statsoft) or Graphpad prism version 5.
Females weaned onto cafeteria diets were heavier than controls at mating (Con 21.5 ± 0.3 g; DIO 28.0 ± 1.4 g; p < 0.001) in agreement with our previous study . There were no differences in gestation length (Con 20.2 ± 0.2; DIO 20.4 ± 0.2 days; p = 0.5) or litter numbers between groups or in birthweight in either sex (Table 1). By weaning, both male and female offspring of DIO were heavier than offspring of Con mothers (Table 1). At 3 and 6 months, male and female CON/DIO and DIO/DIO offspring were heavier than either CON/CON or DIO/CON (repeated measures ANOVA; effect of post-weaning diet: males F(1,28) = 91.3, p < 0.001, females F(1,27) = 97.3, p < 0.001) but there was no additional effect of exposure to maternal overnutrition during pregnancy and lactation. At 6 months, post-weaning exposure to cafeteria diet was associated with increased fat pad weight in both males and females (Table 1) and there was an additional effect of exposure to maternal overnutrition during pregnancy and lactation to increase retroperitoneal fat pad weight (F(1,27) = 5.79, p = 0.02) specifically in female offspring (Table 1).
At 3 months, post-weaning exposure to cafeteria diet increased glucose, insulin (Figure 1A and B) and cholesterol (Table 1) concentrations in males; increased plasma insulin and cholesterol concentrations in females (Figure 1C and D) and increased HOMA-IR in both sexes (Table 1: males: F(1,25) = 60.9, p < 0.001; females F(1,25) = 15.1, p < 0.001). There was an additional effect of exposure to maternal overnutrition to reduce insulin levels in males (Figure 1B. F(1,28) = 5.2, p = 0.03) and to reduce HOMA-IR in females (Table 1: F(1,25) = 4.48, p = 0.044).
At 6 months, post-weaning exposure to cafeteria diet increased glucose, insulin (Figure 2) and cholesterol (Table 1) concentrations and HOMA-IR in both sexes (Table 1: males: F(1,28) = 118.7, p < 0.001; females F(1,26) = 28.7, p < 0.001). There were no persistent effects of exposure to maternal overnutrition during pregnancy and lactation on plasma glucose, insulin or cholesterol levels or HOMA-IR in either sex.
Here we replicate the findings of our earlier study in which we found that maternal overnutrition was associated with few effects in the F1 offspring . Nevertheless, since some studies report that the programming phenotype may only become overt, or is amplified following postnatal exposure to a high-fat diet [11, 12]; we reasoned that post-weaning exposure to a cafeteria diet might result in a more obvious phenotype. As expected, F1 males and females weaned onto the cafeteria diet showed increased weight gain, glucose-insulin dyshomeostasis and hypercholesterolaemia. There were some additional effects of exposure to maternal overnutrition at 3 months which were sex-specific, with reduced insulin levels in males and reduced HOMA-IR in females, suggesting increased insulin sensitivity. However, these effects were no longer present at 6 months, although maternal overnutrition was associated with increased retroperitoneal fat mass in F1 females on the cafeteria diet.
Our results differ from those in number of animal models which document effects of exposure to a maternal high-fat diet on offspring obesity and glucose-insulin homeostasis [7, 13, 14]. Nevertheless, our findings are consistent with reports in some models which show little additional effect of maternal overnutrition on the phenotype induced by post-weaning exposure to an obesogenic diet [15–18]. Notably, in one of these studies, maternal consumption of a high-fat diet was protective against the development of obesity induced by a sucrose-rich diet [17, 18]. Potential mechanisms for the apparent ‘protection’ from the adverse consequences of early life exposure to overnutrition may include differences in the species or strain of animal used, the degree of maternal obesity and/or gestational weight gain, age at mating and whether first or second litters are used. The diets utilised in different studies differ in macro- and micronutrient content and this may be of particular importance in determining effects on the offspring [17, 19, 20]. We used diets matched for micronutrient content, which may represent an additional source of difference between groups in other studies. For example, Couvreur et al. showed that the offspring of females fed a high-fat diet were protected from the development of obesity postnatally, whereas the offspring of mothers maintained on a highly palatable diet were not ; whilst another group reported that different fat sources in the maternal diet had very different influences on the susceptibility of offspring to body weight gain, with prenatal exposure to some fat types providing relative protection from the development of obesity . We have previously documented in our model that maternal protein intake does not differ between groups , however it is possible that in other studies, the dietary protein content differs between obesogenic and control diets, so that plausibly, some of the programmed effects might instead be due to in utero protein restriction which has known programming effects . Additionally, unlike other studies which use an obesogenic diet alongside standard laboratory chow, or which supplement diets with highly palatable substances to increase calorie availability [7, 23], we used diets matched for micronutrient content, which may represent an additional source of difference between groups in other studies. A further possibility is that there may a ‘ceiling effect’ from such a high-fat diet (58% fat), and that more subtle effects may be seen with more moderate diets.
Another explanation for the lack of phenotype in our model could be that the moderate degree of maternal obesity engendered by the cafeteria diet was not severe enough to affect offspring development, however the weights of the dams in our study are consistent with those reported in other studies in which effects on offspring body weight, appetite, glucose-insulin homeostasis and blood pressure are reported [7, 24] and we have previously shown in this model that pregnant females are hyperinsulinaemic and hypercholesterolaemic in late gestation . We have previously suggested that one explanation might be maternal adaptations which occurred during pregnancy, so that although prior to conception, DIO dams were heavier and hyperglycaemic and hyperinsulinaemic when compared to control dams, they gained less weight and were no longer hyperglycaemic or hyperlipidaemic in late gestation [9, 25]. Finally, although other studies report effects are present in F1 offspring by 6 months, it is possible that a phenotype might become more apparent in our model with ageing .
Sex differences in the response of males and females to diet-induced obesity are well-described in the literature  and sex specific effects in prenatal programming paradigms are also common [28–31]. The potential mechanisms have been recently reviewed  and may include sex differences in developmental trajectories and timing and the effects of sex steroids. Additionally, in our study, the effects of post-weaning exposure to an obesogenic diet were sex-specific. Whilst both sexes gained more weight on the cafeteria diet and had a substantial increase in fat pad weight compared to controls, there was a more severe metabolic phenotype in obese males, which showed profound hyperglycaemia and hyperinsulinaemia on glucose tolerance testing whilst females developed less severe hyperinsulinaemia and hyperglycaemia. Although it is well recognised that there are gender differences in cardiovascular disease risk associated with obesity in humans , most studies aimed at dissecting the mechanisms linking obesity and cardiovascular disease and at developing treatment strategies to ameliorate these consequences have been performed in males . The processes accounting for these gender differences are not well defined but sex steroids presumably play a key, albeit complex role .
In conclusion, we show that although post-weaning exposure to a cafeteria diet results in increased adiposity and metabolic derangement in both sexes, exposure to maternal overnutrition in early life has little effect to exacerbate this phenotype. Further studies designed to increase our understanding of what factors lead to the differences between models may help us understand the link between overnutrition in early life and later disease risk in humans and suggest ways to intervene to prevent the complications of exposure to overnutrition in early life.
Barker DJ, Osmond C: Infant mortality, childhood nutrition, and ischaemic heart disease in England and Wales. Lancet. 1986, 1: 1077-1081.
Drake AJ, Reynolds RM: Impact of maternal obesity on offspring obesity and cardiometabolic disease risk. Reproduction. 2010, 140: 387-398.
Hochner H, Friedlander Y, Calderon-Margalit R, Meiner V, Sagy Y, Avgil-Tsadok M, Burger A, Savitsky B, Siscovick DS, Manor O: Associations of maternal prepregnancy body mass index and gestational weight gain with adult offspring cardiometabolic risk factors - The Jerusalem Perinatal Family Follow-Up Study. Circulation. 2012, 125: 1381-1389.
Reynolds RM, Allan KM, Raja EA, Bhattacharya S, McNeill G, Hannaford PC, Sarwar N, Lee AJ, Bhattacharya S, Norman JE: Maternal obesity during pregnancy and premature mortality from cardiovascular event in adult offspring: follow-up of 1 323 275 person years. BMJ. 2013, 347: f4539-
Guelinckx I, Devlieger R, Beckers K, Vansant G: Maternal obesity: pregnancy complications, gestational weight gain and nutrition. Obes Rev. 2008, 9: 140-150.
Li M, Sloboda D, Vickers M: Maternal obesity and developmental programming of metabolic disorders in offspring: evidence from animal models. Exp Diabetes Res. 2011, 2011: 592408-
Samuelsson A-M, Matthews PA, Argenton M, Christie MR, McConnell JM, Jansen EHJM, Piersma AH, Ozanne SE, Twinn DF, Remacle C, Rowlerson A, Poston L, Taylor PD: Diet-induced obesity in female mice leads to offspring hyperphagia, adiposity, hypertension, and insulin resistance: A novel murine model of developmental programming. Hypertension. 2008, 51: 383-392.
Chen H, Simar D, Lambert K, Mercier J, Morris MJ: Maternal and postnatal overnutrition differentially impact appetite regulators and fuel metabolism. Endocrinology. 2008, 149: 5348-5356.
King V, Dakin RS, Liu L, Hadoke PW, Walker BR, Seckl JR, Norman JE, Drake AJ: Maternal obesity has little effect on the immediate offspring but impacts on the next generation. Endocrinology. 2013, 154: 2514-2524.
Reynolds RM, Osmond C, Phillips DIW, Godfrey KM: Maternal BMI, parity, and pregnancy weight gain: Influences on offspring adiposity in young adulthood. J Clin Endocrinol Metab. 2010, 95: 5365-5369.
Rajia S, Chen H, Morris MJ: Maternal overnutrition impacts offspring adiposity and brain appetite markers-modulation by postweaning diet. J Neuroendocrinol. 2010, 22: 905-914.
Chen H, Simar D, Morris MJ: Hypothalamic neuroendocrine circuitry is programmed by maternal obesity: interaction with postnatal nutritional environment. PLoS One. 2009, 4: e6259-
Fernandez-Twinn DS, Blackmore HL, Siggens L, Giussani DA, Cross CM, Foo R, Ozanne SE: The programming of cardiac hypertrophy in the offspring by maternal obesity is associated with hyperinsulinemia, AKT, ERK, and mTOR activation. Endocrinology. 2012, 153: 5961-5971.
Tamashiro KLK, Terrillion CE, Hyun J, Koenig JI, Moran TH: Prenatal stress or high-fat diet increases susceptibility to diet-induced obesity in rat offspring. Diabetes. 2009, 58: 1116-1125.
Gregersen S, Dyrskog SEU, Storlien LH, Hermansen K: Comparison of a high saturated fat diet with a high carbohydrate diet during pregnancy and lactation: effects on insulin sensitivity in offspring of rats. Metabolism. 2005, 54: 1316-1322.
Connor KL, Vickers MH, Beltrand J, Meaney MJ, Sloboda DM: Nature, nurture or nutrition? Impact of maternal nutrition on maternal care, offspring development and reproductive function. J Physiol. 2012, 590: 2167-2180.
Couvreur O, Ferezou J, Gripois D, Serougne C, Crépin D, Aubourg A, Gertler A, Vacher C-M, Taouis M: Unexpected long-term protection of adult offspring born to high-fat fed dams against obesity induced by a sucrose-rich diet. PLoS One. 2011, 6: e18043-
Ferezou-Viala J, Roy A-F, Serougne C, Gripois D, Parquet M, Bailleux V, Gertler A, Delplanque B, Djiane J, Riottot M, Taouis M: Long-term consequences of maternal high-fat feeding on hypothalamic leptin sensitivity and diet-induced obesity in the offspring. Am J Physiol Regul Integr Comp Physiol. 2007, 293: R1056-R1062.
Ainge H, Thompson C, Ozanne SE, Rooney KB: A systematic review on animal models of maternal high fat feeding and offspring glycaemic control. Int J Obes. 2011, 35: 325-335.
Dong Y-M, Li Y, Ning H, Wang C, Liu J-R, Sun C-H: High dietary intake of medium-chain fatty acids during pregnancy in rats prevents later-life obesity in their offspring. J Nutr Biochem. 2011, 22: 791-797.
Sánchez J, Priego T, García AP, Llopis M, Palou M, Picó C, Palou A: Maternal supplementation with an excess of different fat sources during pregnancy and lactation differentially affects feeding behavior in offspring: Putative role of the leptin system. Mol Nutr Food Res. 2012, 56: 1715-1728.
Ozanne SE, Smith GD, Tikerpae J, Hales CN: Altered regulation of hepatic glucose output in the male offspring of protein-malnourished rat dams. Am J Physiol. 1996, 270: E559-E564.
Bayol SA, Farrington SJ, Stickland NC: A maternal 'junk food’ diet in pregnancy and lactation promotes an exacerbated taste for ‘junk food’ and a greater propensity for obesity in rat offspring. Br J Nutr. 2007, 98: 843-851.
Torrens C, Ethirajan P, Bruce KD, Cagampang FRA, Siow RCM, Hanson MA, Byrne CD, Mann GE, Clough GF: Interaction between maternal and offspring diet to impair vascular function and oxidative balance in high fat fed male mice. PLoS One. 2012, 7: e50671-
Pedroni SMA, Turban S, Kipari T, Dunbar DR, McInnes K, Saunders PTK, Morton NM, Norman JE: Pregnancy in obese mice protects selectively against visceral adiposity and is associated with increased adipocyte estrogen signalling. PLoS One. 2014, 9: e94680-
Tarry-Adkins JL, Ozanne SE: The impact of early nutrition on the ageing trajectory. Proc Nutr Soc. 2014, FirstView: 1-13.
Pettersson US, Waldén TB, Carlsson P-O, Jansson L, Phillipson M: Female mice are protected against high-fat diet induced metabolic syndrome and increase the regulatory T cell population in adipose tissue. PLoS One. 2012, 7: e46057-
Dahlhoff M, Pfister S, Blutke A, Rozman J, Klingenspor M, Deutsch MJ, Rathkolb B, Fink B, Gimpfl M, Hrabě de Angelis M, Roscher AA, Wolf E, Ensenauer R: Peri-conceptional obesogenic exposure induces sex-specific programming of disease susceptibilities in adult mouse offspring. Biochim Biophys Acta (BBA) - Mol Basis Dis. 2014, 1842: 304-317.
Schroeder M, Shbiro L, Moran TH, Weller A: Maternal environmental contribution to adult sensitivity and resistance to obesity in long evans rats. PLoS One. 2010, 5: e13825-
Gabory A, Ferry L, Fajardy I, Jouneau L, Gothié J-D, Vigé A, Fleur C, Mayeur S, Gallou-Kabani C, Gross M-S, Attig L, Vambergue A, Lesage J, Reusens B, Vieau D, Remacle C, Jais J-P, Junien C: Maternal diets trigger sex-specific divergent trajectories of gene expression and epigenetic systems in mouse placenta. PLoS One. 2012, 7: e47986-
Gabory A, Roseboom T, Moore T, Moore L, Junien C: Placental contribution to the origins of sexual dimorphism in health and diseases: sex chromosomes and epigenetics. Biol Sex Differ. 2013, 4: 5-
Aiken CE, Ozanne SE: Sex differences in developmental programming models. Reproduction. 2013, 145: R1-R13.
Kautzky-Willer A, Handisurya A: Metabolic diseases and associated complications: sex and gender matter!. Eur J Clin Investig. 2009, 39: 631-648.
Pérez-López FR, Larrad-Mur L, Kallen A, Chedraui P, Taylor HS: Review: Gender differences in cardiovascular disease: hormonal and biochemical influences. Reprod Sci. 2010, 17: 511-531.
This work was supported by Tommy’s. We thank Jon Henderson for expert technical assistance.
The authors declare that they have no competing interests.
VK, JEN, JRS and AJD contributed to study design. VK and AJD performed studies and data analysis. All authors contributed to writing and read and approved the final manuscript.