Table 3 In vitro studies demonstrating the outcome of Vit D treatment of cells isolated from endometriosis lesions
From: Effects of vitamin D supplementation in endometriosis: a systematic review
Author | Country | Cell cultures | Assays used | Sample size | Treatment | Results |
|---|---|---|---|---|---|---|
Delbandi et al., 2016 [37] | Iran | endometriotic stroma cells and eutopic endometrium | XTT proliferation assay, cell invasion assay, cell attachment assay, ELISA, RT-PCR, Western blot | 45 (25 with endometrioma, 20 without endometriosis) | 1,25(OH)2D3 | ↑ cell adhesion ↓ cell invasion, cell proliferation ↓ IL-6 and VEGF-A gene expression by ectopic stroma cells ↓ Bcl-2 gene expression by eutopic and Bcl-xL by ectopic stroma cells |
Miyashita et al. 2016 [13] | Japan | endometriotic stroma cells isolated from ovarian endometrioma | RT-PCR, ELISA, enzyme immunoassay, cell-counting assay, bromodeoxzuridine incorporation assay, Western blot, RIA | 35 | 1,25(OH)2D3 | ↓ IL-1β- or TNF-a-induced inflammatory responses ↓ endometriotic stromal cell numbers ↓ MMP-2 and MMP-9 ↓ DNA synthesis |
Ingles et al., 2017 [38] | USA | endometriotic stroma cells isolated from peritoneal endometriosis lesions | RT-PCR | 43 (38 endometriosis, 5 controls) | 1,25(OH)2D3 | ↑ gene expression of CYP24A1 ↓ neuroangiogenesis ↓ cellular motility ↓ invasion |
Yaghoubi et al., 2020 [39] | Iran | peritoneal fluid mononuclear cells | RT-PCR | 20 (10 women with endometriosis, 10 women without endometriosis) | 1,25(OH)2D3 | Endometriosis ↓ gene expression of epidermal growth factor ↓ gene expression of platelet-derived growth factor-B ↓ gene expression of monocyte/macrophage-derived growth factor Non-endometriosis no significant effect on expression of above genes |