Fig. 4

Distribution of DBH in ovaries of Vh (a) or EV-treated rats (c) and before the bilateral injection with propranolol (Pro) (b-d) into the ovarian bursas. The ovarian sections were stained with anti-DBH antibody (green color), and nuclear staining was performed with DAPI (blue color). e Negative control where the primary antibody was substituted with PBS. DBH is observed throughout the ovary, including the F: follicle, T: theca cell. Bar 100 μm. f ImageJ analysis of DBH relative fluorescence means ± SE (n = 3 animals per group with 10 pseudo-replicas per animal), ^ap < 0.05 vs Vh group; ^bp < 0.05 EV group (one-way analysis of variance, followed by Tukey)