Fig. 3

Distribution of TH in ovaries of Vh (a) or EV-treated rats (c) and before the bilateral injection of propranolol (Pro) (b, d) into the ovarian bursas. e Negative control where the primary antibody was substituted with PBS. The ovarian sections were stained with anti-TH antibody (green color), and nuclear staining was performed with DAPI (blue color). TH is observed throughout the ovary, including the F: follicle and T: Theca cell. Bar 100 μm. f ImageJ analysis of TH relative fluorescence means ± SE (n = 3 animals per group with 10 pseudo-replicas per animal), ^ap < 0.05 vs Vh group; ^bp < 0.05 vs. EV group (one-way analysis of variance, followed by Tukey)