- Open Access
Endometrial TGF-β, IL-10, IL-17 and autophagy are dysregulated in women with recurrent implantation failure with chronic endometritis
- Wen-juan Wang†1Email authorView ORCID ID profile,
- Hong Zhang†2,
- Zhong-quan Chen†3,
- Wei Zhang1,
- Xue-mei Liu1,
- Jiang-ye Fang1,
- Fu-jun Liu4 and
- Joanne Kwak-Kim5Email author
© The Author(s). 2019
- Received: 15 October 2018
- Accepted: 12 December 2018
- Published: 3 January 2019
Chronic endometritis (CE) is a condition which results in reduced receptivity of embryos by dysregulated lymphocyte subsets, abnormal expression of cytokines, chemokines and other regulatory molecules in the endometrium (EM). Macroautophagy (autophagy), the highly conserved cellular homeostasis pathway, plays an essential role in the development and function of T lymphocytes, and supports T cell lineage stability and survival fitness. The possible relationships between autophagy and local cytokine milieus in repeated implantation failure (RIF) with CE have not been elucidated yet.
This case-control study was performed at a large reproductive medicine center between February 2015 and July 2016. Seventy-five recurrent implantation falliure women with CE who had “strawberry aspect” and 75 women with male factor infertility were included. In this study, endometrial expressions of IL-17, IL-10, TGF-β and autophagy related molecules, including LC3-II and mTORC1 were investigated by qRT-PCR, Western blot, immunofluorescence and immunohistochemistry assays.
The expression of IL-17 was significantly higher in patients with CE compared to women with male factor infertility, while the expressions of IL-10 and TGF-β were significantly lower. Moreover, the expression of autophagy (LC3-II) is increased, while the expression of mTORC1 was impaired.
CE is associated with shifted cytokine milieu towards Th17 over Treg immunity in endometrium through impaired autophagy by decreased mTORC1.
- Repeated implantation failure
- Chronic endometritis
Repeated implantation failure (RIF) is defined as a failure to achieve a clinical pregnancy after the transfer of three or more good-quality embryos in women < 35 years of age, and four or more good quality embryos in women ≥35 years during fresh or frozen embryo transfer cycles . Successful implantation and maintenance of pregnancy require a delicate balance between pro- and anti-inflammatory immune responses at the maternal fetal interface . Endometrial cytokines and chemokines act in complex networks and orchestrate the changes in endometrial leukocyte populations, which play a major role in vascular remodeling and angiogenesis [3, 4]. Chronic endometritis (CE) however, often induces altered endometrial cytokine and chemokine productions, and endometrial dysfunction . In addition, these changes accompany abnormal patterns of lymphocyte subsets and altered secretion of paracrine factors. Hence, CE is often associated with reduced endometrial receptivity to invading embryos and recurrent pregnancy losses [6–8].
In women with CE which is a chronic inflammatory condition, endometrial immune responses are often shifted towards pro-inflammatory profiles and consequently, become unfavorable to invading embryos . Previously, decreased expression of transforming growth factor-β (TGF-β) and interleukin (IL)-10 mainly secreted by T helper (Th) 2, T regulatory cells (Tregs) or alternatively activated macrophage (M2), and increased expression of IL-17 by Th17 cells have been reported to participate in maternal immune rejection of the fetus [10–12]. Hence, a possible assault to the fetus by maternal immune responses can be largely prevented by suppressing Th1 or Th17 immune activation [13–15]. Enhancement of local and systemic Th2 immunity and tolerogenic Tregs can be a possible strategy to suppress the pro-inflammatory immune responses.
The highly conserved cellular homeostasis pathway, autophagy, degrades large protein aggregates, removes damaged or extraneous organelles, recycles nutrients, and promotes cell survival during stressful conditions [16–18]. Autophagy plays an essential role in the differentiation of T lymphocytes and its function. Autophagy is active in Tregs and supports their lineage stability and survival fitness. Genetic or pharmacological alterations in autophagy impair cell survival rate or its metabolism, thereby affecting tissue homeostasis . Decreased expression of autophagy is associated with poor development of embryo and implantation failure . Moreover, Autophagy is physiologically involved in early normal gestation . Microtubule-associated protein 1A/1B-light chain 3 (LC3), is a soluble protein that is distributed ubiquitously in mammalian tissues. LC3 exists in two forms: a cytosolic form (LC3-I) and a lipid phosphatidylethanolamine-conjugated form (LC3-II) that is inserted into both inner and outer membranes of the growing autophagosome. LC3-II, the phosphatidylethanolamine conjugated product of LC3-I obtained after LC3 activation is currently used as a specific marker for autophagy due to its role in autophagosome genesis . mTOR, a master regulator of cellular metabolism, was reported to have a crucial role in regulating cellular autophagy [23–26]. mTOR integrates signals from the environment to the nucleus for the regulation of cell metabolism, proliferation, survival and autophagy . Indeed, mTOR signaling in placenta was positively correlated with birth weight of the infant . Therefore, a balance between autophagy and mTOR may be crucial for successful pregnancy outcome.
Whether the expression of autophagy and mTOR is associated with cytokine network in human endometrium has not been studied well. Considering the importance of IL-10, IL-17 and TGF-β in maternal-fetal immune tolerance, we aim to analyze the endometrial expression of these cytokines and autophagy related molecules, such as LC3II and mTOR1 in women with RIF and CE.
(n = 75)
Control group (n = 75)
Mean age (years)
32.4 ± 0.3
32.1 ± 0.4
Median body mass index (kg/cm2)
23.5 ± 0.4
24.8 ± 0.8
0.5 ± 0.1
0.3 ± 0.1
Infertility history (years)
3.5 ± 0.1
2.3 ± 0.1
Number of failed ET cycles
4.0 ± 0.2
(P < 0.05)
Number of total embryos transferred
3.2 ± 0.2
(P < 0.05)
History of bacterial or tuberculous pelvic inflammation (years)
Evaluation of CE by hysteroscope
Office hysteroscopy was scheduled during the follicular phase (between cycle day 8 to 12) of the menstrual cycle. The procedure was performed using a rigid hysteroscope consists of a telescope with 3 mm outer diameter and 30 degree fore-oblique lens, and a 3-CCD digital camera system (Karl Storz GmbH & Co. KG, Tuttlingen, Germany). A 50-W Hi-Lux light source and a 15-in. video color TELE PACK X LED monitor were used. The exploration of the uterine cavity dwelled on a panoramic view of the cavity followed by a thorough evaluation of the endometrial mucosa, as described previously . All hysteroscopies were performed by the one investigator.
Clinical diagnosis of CE was based on the demonstration of micropolyps (< 1 mm) that fluctuate in the cavity, and the presence of hyperemic endometrium flushed with a white central point, localized or scattered throughout the cavity, referred as “strawberry aspect” [29–32]. All women with clinical CE by hysteroscope underwent endometrial biopsy using a curette for histological confirmation.
Hematoxylin-eosin staining for the evaluation of CE
Endometrial samples were fixed in neutral formaldehyde solution and later embedded in paraffin for histological analysis. The micro-sections were stained with hematoxylin and eosin. The histological examinations were performed by the single operator who was unaware of the hysteroscopic findings, and the presence of following features were investigated; superficial stromal edema, increased stromal density, pleomorphic stromal inflammatory infiltrates dominated by lymphocytes and plasma cells.
Investigation of autophage by immunohistochemistry and immunofluorescence
Antigens were unmasked by microwaving sections in 10 μmol/L citrate buffer with pH 6.0, for 15 min, and immunostaining was undertaken using the Rabbit - enhanced polymer detection system with anti- LC3B (bs-2912R, Beijing Biosynthesis Biotechnology Co. Ltd.; dilution 1:800). Color development was performed using HistostainTM-Plus Kits (IgG /Bio, S-A/HRP, DAB, Beijing Zhongshan Goldenbridge Company, China) as a chromogen. After staining, sections were dehydrated through increasing concentrations of ethanol and xylene.
Immunofluorescence studies were conducted in the tissue sections with 4-μm thickness. Primary antibodies were rabbit anti- LC3B (bs-2912R, Beijing Biosynthesis Biotechnology Co. Ltd.; dilution 1:800). FITC conjugated goat anti-rabbit IgG (H + L) (#A22120, Abbkine, Inc.; dilution 1:1200) was utilized as a secondary antibody.
Quantitative real-time PCR (qRT-PCR)
Total RNA was extracted from decidua with the Rneasy Mini Kits (QIAGEN, Valencia, CA) according to the manufacturer’s instructions. For qRT-PCR, amplification was performed in an ABI5700 (PE Biosystems, Foster City, CA) using the SYBR Green kit (QuantiTect SYBR Green PCR; QIAGEN). The primers were designed from the target human mRNA sequence using Primer Express software (Applied Biosystems). Each primer was entered into an NCBI BLAST search to ensure that it was specific for the target mRNA transcription. The primer sequences are the following: IL-17 forward 5’-CCG GAC TGT GAT GGT CAA-3′, reverse 5′- CTC ATT GCG GTG GAG ATT-3′; IL-10 forward 5’-GAC TTT AAG GGT TAC CTG GGT TG-3′, reverse 5’-TCA CAT GCG CCT TGA TGT CTG-3′; TGF-β1 forward 5’-CAA TTC CTG GCG ATA CCT CAG, reverse 5’-GCA CAA CTC CGG TGA CAT CAA-3′. The housekeeping gene β-actin primers, forward 5′- ACG TTG CTA TCC AGG CTG TGC TAT-3′, and reverse 5′-TTA ATG TCA CGC ACG ATT TCC CGC-3′ were used with all samples. The primers were synthesized by BioAsia Co. (Shanghai, China). The cycling conditions were 15 s at 95 °C, 45 cycles of 5 s at 95 °C, 20s at 60 °C, 10s at 72 °C, and 15 s at 65 °C. Data were analyzed using the GeneAmp 5700 Sequence Detection System software (version 1.1; Applied Biosystems, Foster City, CA) and were converted into threshold cycle (Ct) values.
Endometrial tissues were isolated and washed in cold phosphate-buffered saline (PBS) in pH 7.4, then the tissues were minced in lysis buffer (7 M urea, 2 M thiourea, 30 mM Tris-pH 8.5) containing 50 mM DTT and protease inhibitors at 4 °C for 20 min by applying gentle pressure. The homogenate was then centrifuged at 12,000 g for 15 min, and the collected supernatants were precipitated by 4-fold ice-cold acetone, stored for 1 h at − 20 °C, and centrifuged at 12,000 g for 20 min at 4 °C. Precipitates were washed with 90% ice-cold acetone, dissolved in lysis solution and protein concentrations were determined by the Bradford assay (Bio-Rad, USA). An equal amount of proteins (50 μg) from each sample were separated by 12% SDS-PAGE and were transferred to polyvinylidene difluoride membranes, blocked with 5% (w/v) skimmed milk for 1 h at room temperature (RT), then incubated for 1 h with primary antibody at RT with gentle agitation. After washing with 0.5% (v/v) Tween-20 in Tris-buffered saline for three times, membranes were incubated for 1 h at RT with HRP-conjugated anti-IgG at a final dilution of 1:5000 in TBST buffer. Immunoreactive complexes were visualized using ECL Western Blotting substrate kit (Rockford, IL, USA). Western blot images were quantified by the densitometric scanning with ImageQuant TL 7.0 software (GE Healthcare, USA).
Data are presented as the means ± SEM. Statistical analyses were performed using GraphPad Prism 5 (GraphPad Software Inc.; LaJolla, CA, USA). The Student’s t test was used to compare differences between the two groups. A P value of < 0.05 was considered statistically significant.
Hysteroscopic finding of CE and histopathology of endometrium
mRNA and protein expression of IL-17, IL-10 and TGF-β in endometrium
Western blot analysis was performed to explore the endometrial expression of IL-10, TGF-β and IL-17 (N = 15 for each). The level of IL-17 proteins (0.8 ± 0.1 vs 0.5 ± 0.06) was significantly higher, while the levels of IL-10 (0.5 ± 0.06 vs 0.8 ± 0.11) and TGF-β (0.5 ± 0.05 vs 1.1 ± 0.08) were significantly lower in the CE group than in controls (P < 0.05 respectively) (Fig. 3b). Moreover, the ratio of IL-17/IL-10 in the CE group (2.05 ± 0.52) was significantly higher than that of the controls (0.85 ± 0.14) (P < 0.05). The ratio of IL-17/TGF-β in the CE group (1.65 ± 0.22) was significantly higher than that of controls (0.54 ± 0.10) (P < 0.05) (Fig. 3c).
The expression of autophagy related proteins, LC3-II and mTORC1 in endometrium
In this study, we report that women with RIF and CE have decreased endometrial TGF-β and IL-10 expression and increased IL-17 expression, accompanied by increased autophage. These findings support a notion that CE is associated with increased pro-inflammatory immune responses, which is often related to poor reproductive outcome, such as recurrent pregnancy losses (RPL) or RIF .
CE is usually asymptomatic and associated with infection, incomplete pregnancy loss or retained placental tissue . Although antibiotic treatment has been frequently recommended, often a causal organism cannot be detected. CE was reported in 14% of women with RPL and 11% of fetal death group. Per-pregnancy live birth rate without the treatment was 7% vs. 56% with treatment . In this study we found 43.4% of women with RIF have CE, which is higher than those of women with RPL or fetal death.
The density and phenotype of lymphocytes in peripheral tissues are generally regulated by local immunoregulatory cytokines, particularly those influencing antigen-presenting cell (APC) function , and phenotype acquisition and maintenance in T helper cells . Chronic inflammation may induce an abnormal local immune-regulatory cytokines and alter the pattern of CD4+ T cells. During pregnancy, CD4+ T cells provide a receptive environment for the development of the semi-allogenic conceptus by balancing Th1/Th2 and Th17/Treg cell immune responses . Tregs contribute to the maintenance of tolerance during implantation and suppress maternal alloreactive immune responses against paternal antigens in trophoblasts by actively suppressing self-reactive lymphocytes via mechanisms that are mediated by a cell to cell contact and production of soluble factors, such as TGF-β and IL-10 [10–12]. In this study, women with CE had significantly decreased TGF-β and IL-10 expression in endometrium, which reflect numerical or functional deficiency of Treg cells. Previously, it has been reported that in the absence or the functional deficiency of Tregs, inflammation and fibrosis occur, and blastocysts fail to implant [39, 40]. In our previous study, we demonstrated that decreased expression of Tregs at the maternal-fetal interface in mice with impaired implantation . Contrarily, the periodic accumulation of Tregs during the receptive phase plays an essential role for the proper establishment of pregnancy .
Th17 and Tregs present a certain level of plasticity, which has diverse fates in different inflammatory frameworks [43, 44]. Under inflammatory circumstances, Tregs can trans-differentiate into Th17 cells, while Th17 cells that present plasticity towards Th1 have higher survival rate and less senescence than Th1 cells [45, 46]. However, the expression characteristics of Th17 and Treg cells in CE have not been reported. In the present study, we demonstrated significantly increased IL-17 and decreased IL-10 and TGF-β expressions in endometrium of CE patients. This suggests CE induces a propensity to Th17 over Treg immunity in endometrium, which consequently, leads to poor reproductive outcome, such as RIF or RPL.
Autophagy is a highly conserved mechanism of lysosome-mediated protein degradation that plays a key role in maintaining cellular homeostasis by recycling amino acids, reducing the amount of damaged proteins, and regulating protein levels in response to extracellular signals . Autophagy has important effects on the induction and modulation of the inflammatory reaction . In this study, for the first time we detected significantly increased expression of autophagy related protein LC3-II in the endometrium of CE as compared to normal controls. Increased numbers of LC3-containing vesicles and increased LC3 flux indicate active autophagosome formation and clearance [22, 49]. In CE endometrium, autophagy was mainly present in the endometrial stroma where lymphocytes are residing, but not in the endometrial glands. These findings may suggest that autophagy has important effects on the induction of inflammatory reaction in CE and sequential changes in local cytokine milieu. Indeed, excessively stimulated autophage was reported to lead to endothelial cell death that can contribute to plaque destabilization and maintaining inflammatory status of the plaque in patients with atherosclerosis .
Autophagy markers are present in cytotrophoblast, syncytiotrophoblast, extravillous trophoblast and decidual stromal cells, and physiologically involved in early gestation. Impaired autophagy at the feta-maternal interface contributes to the pathophysiology of abortion and preeclampsia [27, 51]. The mammalian Target of Rapamycin (mTOR) is an evolutionarily conserved member of the phosphatidylinositol-3-OH-kinase-related kinase (PI3KK) family that plays a central role in the regulation of metabolism, protein synthesis, energy balance, proliferation and survival. mTOR forms the core of two distinct signaling complexes mTORC1 and mTORC2, of which activation are deferentially regulated . mTORC1 can promote lipid biogenesis and energy metabolism through the activation of transcription factors and repress autophagy . In this study, we report for the first time that the expression of mTORC1 was significantly down-regulated in the CE group. It has been reported that mTOR modifies inflammatory responses by modulating immunoproteasomal degradation , and inflammatory responses induced by LPS was suppressed by PI3K/AKT/mTOR pathway. Hence, it is postulated that decreased mTORC1 in CE induces increased autophagy and local pro-inflammatory immune responses .
In conclusion, the abnormal expression of IL-10, TGF-β and IL-17 in RIF women with CE, combined with decreased level of autophagy demonstrate the presence of endometrial proinflammatory immune responses, which is associated with a decreased endometrial receptivity and pregnancy rate. These results suggested that by regulating autophagy, it may be able to regulate local immune responses and improve the implantation rate in recurrent implantation failure patients with endometritis. Well-designed epidemiological studies are warranted to verify these findings.
Research reported in this publication was supported by Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
This work was supported by grants from the National Natural Science Foundation of China (grant numbers 81741027, 81300533, 81601276), Chinese Medical Association Clinical Medicine Research Special Fund-2017, Reproductive Medicine Young Physicians Research and Development project (17020160685, 16020220638), and Yantai Key research and development program (2017YT06000491). MerckSerono China Research Fund for Fertility Experts (2014).
Availability of data and materials
The primary data for this study is available from the authors on direct request.
W.W.J was responsible for the study design and manuscript drafting. Z.H and C.Z.Q was responsible for the laboratory operation, data acquisition and analysis. Z.W was responsible for hysteroscopy. L.X.M., F.J.Y. and L.F.J. were responsible for the specimen collection, data interpretation and critical discussion. J.K.K was responsible for the study design, data analysis and manuscript writing. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The study was approved by the Research Ethics Committee of Yantai Yuhuangding Hospital.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Coughlan C, Ledger W, Wang Q, Liu F, Demirol A, Gurgan T, Cutting R, Ong K, Sallam H, Li TC. Recurrent implantation failure: definition and management. Reprod BioMed Online. 2014;28:14–38.View ArticleGoogle Scholar
- Liu S, Diao L, Huang C, Li Y, Zeng Y, Kwak-Kim JYH. The role of decidual immune cells on human pregnancy. Reprod Immunol. 2017;124:44–53.View ArticleGoogle Scholar
- Dunk C, Smith S, Hazan A, Whittle W, Jones RL. Promotion of angiogenesis by human endometrial lymphocytes. Immunol Investig. 2008;37:583–610.View ArticleGoogle Scholar
- Schatz F, Guzeloglu-Kayisli O, Arlier S, Kayisli UA, Lockwood CJ. The role of decidual cells in uterine hemostasis, menstruation, inflammation, adverse pregnancy outcomes and abnormal uterine bleeding. Hum Reprod Update. 2016;22:497–515.View ArticleGoogle Scholar
- Maybin JA, Critchley HO, Jabbour HN. Inflammatory pathways in endometrial disorders. Mol Cell Endocrinol. 2011;335:42–51.View ArticleGoogle Scholar
- Matteo M, Cicinelli E, Greco P, Massenzio F, Baldini D, Falagario T, Rosenberg P, Castellana L, Specchia G, Liso A. Abnormal pattern of lymphocyte subpopulations in the endometrium of infertile women with chronic endometritis. Am J Reprod Immunol. 2009;61:322–9.View ArticleGoogle Scholar
- Di Pietro C, Cicinelli E, Guglielmino MR, Ragusa M, Farina M, Palumbo MA, Cianci A. Altered transcriptional regulation of cytokines, growth factors, and apoptotic proteins in the endometrium of infertile women with chronic endometritis. Am J Reprod Immunol. 2013;69:509–17.View ArticleGoogle Scholar
- Bouet PE, El Hachem H, Monceau E, Gariepy G, Kadoch IJ, Sylvestre C. Chronic endometritis in women with recurrent pregnancy loss and recurrent implantation failure: prevalence and role of office hysteroscopy and immunohistochemistry in diagnosis. Fertil Steril. 2016;105:106–10.View ArticleGoogle Scholar
- Park HJ, Kim YS, Yoon TK, Lee WS. Chronic endometritis and infertility. Clin Exp Reprod Med. 2016;43:185–92.View ArticleGoogle Scholar
- Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. Nat Immunol. 2003;4:330–6.View ArticleGoogle Scholar
- Khattri R, Cox T, Yasayko SA, Ramsdell F. An essential role for Scurfin in CD4+CD25+ T regulatory cells. Nat Immunol. 2003;4:337–42.View ArticleGoogle Scholar
- Hori S, Nomura T, Sakaguchi S. Pillars. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003;299:1057–61.View ArticleGoogle Scholar
- Thellin O, Coumans B, Zorzi W, Igout A, Heinen E. Tolerance to the foeto-placental ‘graft’: ten ways to support a child for nine months. Curr Opin Immunol. 2000;12:731–7.View ArticleGoogle Scholar
- Wang WJ, Hao CF, Qu QL, Wang X, Qiu LH, Lin QD. The deregulation of regulatory T cells on interleukin-17-producing T helper cells in patients with unexplained early recurrent miscarriage. Hum Reprod. 2010;25:2591–6.View ArticleGoogle Scholar
- Wang WJ, Hao CF, Yi-Lin YGJ, Bao SH, Qiu LH, Lin QD. Increased prevalence of T helper 17 (Th17) cells in peripheral blood and decidua in unexplained recurrent spontaneous abortion patients. J Reprod Immunol. 2010;84:164–70.View ArticleGoogle Scholar
- Yang Z, Klionsky DJ. Eaten alive: a history of macroautophagy. Nat Cell Biol. 2010;12:814–22.View ArticleGoogle Scholar
- Mizushima N, Komatsu M. Autophagy: renovation of cells and tissues. Cell. 2011;147:728–41.View ArticleGoogle Scholar
- Deretic V, Saitoh T, Akira S. Autophagy in infection, inflammation and immunity. Nat Rev Immunol. 2013;13:722–37.View ArticleGoogle Scholar
- Wei J, Long L, Yang K, et al. Autophagy enforces functional integrity of regulatory T cells by coupling environmental cues and metabolic homeostasis. Nat Immunol. 2016;17:277–85.View ArticleGoogle Scholar
- Tsukamoto S, Kuma A, Murakami M, Kishi C, Yamamoto A, Mizushima N. Autophagy is essential for preimplantation development of mouse embryos. Science. 2008;321:117–20.View ArticleGoogle Scholar
- Avagliano L, Terraneo L, Virgili E, Martinelli C, Doi P, Samaja M, Bulfamante GP, Marconi AM. Autophagy in normal and abnormal early human pregnancies. Reprod Sci. 2015;22:838–44.View ArticleGoogle Scholar
- Tanida I, Ueno T, Kominami E. LC3 and autophagy. Methods Mol Biol. 2008;445:77–88.View ArticleGoogle Scholar
- Noda T, Fujita N, Yoshimori T. The late stages of autophagy: how does the end begin? Cell Death Differ. 2009;16:984–90.View ArticleGoogle Scholar
- Kim J, Kundu M, Viollet B, Guan KL. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. Nat Cell Biol. 2011;13:132–41.View ArticleGoogle Scholar
- Jung CH, Ro SH, Cao J, Otto NM, Kim DH. mTOR regulation of autophagy. FEBS Lett. 2010;584:1287–95.View ArticleGoogle Scholar
- Sengupta S, Peterson TR, Sabatini DM. Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress. Mol Cell. 2010;40:310–22.View ArticleGoogle Scholar
- Wood SC, Seeley RJ, Cota D. Regulation of food intake through hypothalamic signaling networks involving mTOR. Annu Rev Nutr. 2008;28:295–331.View ArticleGoogle Scholar
- Jansson N, Rosario FJ, Gaccioli F, Lager S, Jones HN, Roos S, Jansson T, Powell TL. Activation of placental mTOR signaling and amino acid transporters in obese women giving birth to large babies. J Clin Endocrinol Metab. 2013;98:105–13.View ArticleGoogle Scholar
- Resta L, Palumbo M, Rossi R, Piscitelli D, Grazia Fiore M, Cicinelli E. Histology of micro polyps in chronic endometritis. Histopathology. 2012;60:670–4.View ArticleGoogle Scholar
- Cicinelli E, Resta L, Nicoletti R, Zappimbulso V, Tartagni M, Saliani N. Endometrial micropolyps at fluid hysteroscopy suggest the existence of chronic endometritis. Hum Reprod. 2005;20:1386–9.View ArticleGoogle Scholar
- Cicinelli E, Resta L, Nicoletti R, Tartagni M, Marinaccio M, Bulletti C, Colafiglio G. Detection of chronic endometritis at fluid hysteroscopy. J Minim Invasive Gynecol. 2005;12:514–8.View ArticleGoogle Scholar
- Cicinelli E, De Ziegler D, Nicoletti R, Colafiglio G, Saliani N, Resta L, Rizzi D, De Vito D. Chronic endometritis: correlation among hysteroscopic, histologic, and bacteriologic findings in a prospective trial with 2190 consecutive office hysteroscopies. Fertil Steril. 2008;89:677–84.View ArticleGoogle Scholar
- Kwak-Kim JY, Chung-Bang HS, Ng SC, Ntrivalas EI, Mangubat CP, Beaman KD, Beer AE, Gilman-Sachs A. Increased T helper 1 cytokine responses by circulating T cells are present in women with recurrent pregnancy losses and in infertile women with multiple implantation failures after IVF. Hum Reprod. 2003;18:767–73.View ArticleGoogle Scholar
- Haggerty CL, Peipert JF, Weitzen S, Hendrix SL, Holley RL, Nelson DB, Randall H, Soper DE, Wiesenfeld HC, Ness RB. PID Evaluation and Clinical Health (PEACH) Study Investigators. Predictors of chronic pelvic pain in an urban population of women with symptoms and signs of pelvic inflammatory disease. Sex Transm Dis. 2005;32:293–9.View ArticleGoogle Scholar
- McQueen DB, Bernardi LA, Stephenson MD. Chronic endometritis in women with recurrent early pregnancy loss and/or fetal demise. Fertil Steril. 2014;101:1026–30.View ArticleGoogle Scholar
- Moser M, Murphy KM. Dendritic cell regulation of TH1-TH2 development. Nat Immunol. 2000;1:199–205.View ArticleGoogle Scholar
- Reiner SL. Helper T cell differentiation, inside and out. Curr Opin Immunol. 2001;13:351–5.View ArticleGoogle Scholar
- Polese B, Gridelet V, Araklioti E, Martens H, Perrier d'Hauterive S, Geenen V. The Endocrine Milieu and CD4 T-Lymphocyte Polarization during Pregnancy. Front Endocrinol (Lausanne). 2014;5:106.View ArticleGoogle Scholar
- Zenclussen AC, Hämmerling GJ. Cellular Regulation of the Uterine Microenviroment That Enables Embryo Implantation. Front Immunol. 2015;6:321.View ArticleGoogle Scholar
- Saxena D, Misra MK, Parveen F, Phadke SR, Agrawal S. The transcription factor Forkhead box P3 gene variants affect idiopathic recurrent pregnancy loss. Placenta. 2015;36:226–31.View ArticleGoogle Scholar
- Wang WJ, Liu FJ, Xin-Liu HCF, Bao HC, Qu QL, Liu XM. Adoptive transfer of pregnancy-induced CD4+CD25+ regulatory T cells reverses the increase in abortion rate caused by interleukin-17 in the CBA/J x BALB/c mouse model. Hum Reprod. 2014;29:946–52.View ArticleGoogle Scholar
- Wang WJ, Liu FJ, Zhang X, Liu XM, Qu QL, Li FH, Zhuang LL, Li XX, Hao CF. Periodic elevation of regulatory T cells on the day of embryo transfer is associated with better in vitro fertilization outcome. J Reprod Immunol. 2017;119:49–53.View ArticleGoogle Scholar
- Kleinewietfeld M, Hafler DA. The plasticity of human Treg and Th17 cells and its role in autoimmunity. Semin Immunol. 2013;25:305–12.View ArticleGoogle Scholar
- Hirota K, Duarte JH, Veldhoen M, Hornsby E, Li Y, Cua DJ, Ahlfors H, Wilhelm C, Tolaini M, Menzel U, Garefalaki A, Potocnik AJ, Stockinger B. Fate mapping of IL-17-producing T cells in inflammatory responses. Nat Immunol. 2011;12:255–63.View ArticleGoogle Scholar
- Wan YY, Flavell RA. Regulatory T-cell functions are subverted and converted owing to attenuated Foxp3 expression. Nature. 2007;445:766–70.View ArticleGoogle Scholar
- Muranski P, Borman ZA, Kerkar SP, Klebanoff CA, Ji Y, Sanchez-Perez L, Sukumar M, Reger RN, Yu Z, Kern SJ, Roychoudhuri R, Ferreyra GA, Shen W, Durum SK, Feigenbaum L, Palmer DC, Antony PA, Chan CC, Laurence A, Danner RL, Gattinoni L, Restifo NP. Th17 cells are long lived and retain a stem cell-like molecular signature. Immunity. 2011;35:972–85.View ArticleGoogle Scholar
- Komatsu N, Okamoto K, Sawa S, Nakashima T, Oh-hora M, Kodama T, Tanaka S, Bluestone JA, Takayanagi H. Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis. Nat Med. 2014;20:62–8.View ArticleGoogle Scholar
- Netea-Maier RT, Plantinga TS, van de Veerdonk FL, Smit JW, Netea MG. Modulation of inflammation by autophagy: consequences for human disease. Autophagy. 2016;12(2):245–60.View ArticleGoogle Scholar
- He C, Klionsky DJ. Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genet. 2009;43:67–93.View ArticleGoogle Scholar
- Martinet W, De Meyer GR. Autophagy in atherosclerosis: a cell survival and death phenomenon with therapeutic potential. Circ Res. 2009;104:304–17.View ArticleGoogle Scholar
- Nakashima A, Yamanaka-Tatematsu M, Fujita N, Koizumi K, Shima T, Yoshida T, Nikaido T, Okamoto A, Yoshimori T, Saito S. Impaired autophagy by soluble endoglin, under physiological hypoxia in early pregnant period, is involved in poor placentation in preeclampsia. Autophagy. 2013;9:303–16.View ArticleGoogle Scholar
- Delgoffe GM, Pollizzi KN, Waickman AT, Heikamp E, Meyers DJ, Horton MR, Xiao B, Worley PF, Powell JD. The kinase mTOR regulates the differentiation of helper T cells through the selective activation of signaling by mTORC1 and mTORC2. Nat Immunol. 2011;12:295–303.View ArticleGoogle Scholar
- Zhang B, Fang L, Wu HM, Ding PS, Xu K, Liu R. Mer receptor tyrosine kinase negatively regulates lipoteichoic acid-induced inflammatory response via PI3K/Akt and SOCS3. Mol Immunol. 2016;76:98–107.View ArticleGoogle Scholar
- Xu L, Brink M. mTOR, cardiomyocytes and inflammation in cardiac hypertrophy. Biochim Biophys Acta. 2016;1863(7 Pt B):1894–903.View ArticleGoogle Scholar