Cationic antimicrobial peptide, magainin down-regulates secretion of pro-inflammatory cytokines by early placental cytotrophoblasts

Table 3 Summary of top-scored features from reports of enrichment and pathways-networks analysis

Analysis	Description of the process (p value)	Candidates^d
Gene ontology (GO) cellular process^a	Inflammatory response (5.9E-12)	CCL3, CCL4, CCL5, CXCL1, IL1A, IL1B, IL6, IL9, IL18, IL-1RA, IL-2RA, CXCL9, CXCL10, MIF, TNF
	Immune response (6.3E-12)	CCL3, CCL4, CCL5, G-CSF, CXCL1, CXCL10, IL1A, IL1B, IL6, IL9, IL18, IL1RA, IL-2RA, LIF, MIF, TNF, VEGF
	Regulation of cell proliferation (6.3E-12)	CCL3, CCL4, CCL5, HGF, GCSF, CXCL1, CXCL9, CXCL10, IL1A, IL1B, IL6, IL9, IL18, IL-1RA, IL-2RA, LIF, MIF, TNF, VEGF
	Response to wound healing (6.4E-12)	CCL3, CCL4, CCL5, CXCL1, CXCL9, CXCL10, HGF, IL1A, IL1B, IL6, IL9, IL18, IL-1RA, MIF, TNF, VEGF
	Cell chemotaxis (6.5E-12)	CLEC11A, CCL4, CCL5, CXCL1, CXCL9, CXCL10, HGF, IL1B, IL6, IL-2RA, VEGF
Enrichment by pathways map^b	Cyto/chemokines in inflammation (3.8E-12)	CCL4, CCL5, CXCL10, IL1A, IL1B, IL6, IL9, TNF
	Secreted signals in immune response (3.9E-12)	CCL4, CCL5, CXCL10, IL1A, IL1B, IL-1RA, IL- 2RA, IL6, IL9, TNF
Most relevant network^c	Toll-like receptor signaling pathway (2.3E-12)	IL1A, IL1B, ΙL6, MIF, TNF

^asources: ebi.ac.uk/quickGO/, Portal.genego.com/cgi/, and Amigo.geneontology.org/amigo
^btop scored pathways were identified using input list of selected candidates showing differential levels on Metacore GeneGO portal (MetaCore Biomanual 5.0. at https://portal.genego.com/help/MetaCpre_bio_manual_5) as discussed elsewhere [11, 17, 18]. Source: portal.genego.com/
^cThe cyto/chemokines displaying differential secretion following administration of AMA as shown in Table 1 used as the input list for generation of biological networks using Analyze Networks (AN) algorithm with default settings. This is a variant of the shortest paths algorithm with main parameters of (i) relative enrichment with the uploaded data from Table 1 and (ii) relative saturation of networks with canonical pathways. These networks are built on the fly and unique for the uploaded data. In this workflow the networks are prioritized based on the number of fragments of canonical pathways on the network as discussed elsewhere [17, 18]. Source: Portal.genego.com/
^dCyto/chemokines detected in all groups. The negatively affected candidates by AMA are shown in italics and positively affected ones are shown in bold

ISSN: 1477-7827