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Table 2 Comparative profiles of immunopositive proteins in medium and respective transcripts in cells for selected cytokines in primary cultures with (1000 ng/ml) and without AMA

From: Cationic antimicrobial peptide, magainin down-regulates secretion of pro-inflammatory cytokines by early placental cytotrophoblasts

Name of cytokine

Mean ± SD (n = 5/ea)

 

Concentration in conditioned mediuma

Relative level of transcript in cellsb

 

Control

AMA

Control

AMA

CCL4 (MIP1B)

298.0

260.2**

2.5

2.6

 

±22.1

±12.4

±0.7

±0.9

CCL5 (RANTES)

362.4

309.7*

5.2

5.8

 

±20.7

±11.6

±2.7

±2.1

IL1A

113.4

120.6

2.9

3.6

 

±14.9

±13.4

±2.4

±2.1

IL1B

353.0

302.2**

2.5

2.9

 

±23.4

±10.6

±1.1

±1.5

IL-2RA

0.08c

2.9***

7.3

9.5**

 

±0.03

±0.1

±0.1

±0.3

IL6

752.7

587.8**

5.8

6.9

 

±41.8

±21.1

±1.2

±1.8

IL16

11.4

18.8*

7.5

8.8**

 

±3.9

±2.2

±0.1

±0.2

MIF

2919.2

2154.2

9.8

8.3*

 

±178.5

±143.0

±0.6

±0.4

TNF

0.6

0.4

3.0

3.1

 

±0.3

±0.3

±0.5

±0.8

  1. apg per mg Bradford protein from Bio-plex immunoassays and as shown in Table 1. bΔΔCt relative to housekeeping genes (GAPDH and UBC) based on real time RT-PCR using SYBR green uniplex chemistry. *P < 0.05, **P < 0.01, ***P < 0.001 as compared to control. cvalues <0.1 pg/mg Bradford protein considered not detectable