Fig. 3

Representative Western immunoblots of CCL4 and TNF from conditioned media without cells (Control, − cells/+AMA), and with cytotrophoblast cells grown in primary culture on collagen biomatrix (+ cells) with AMA (1000 ng/ml) and without AMA (Control, +cells). Media containing 20 μg of Bradford proteins were subjected to electrophoretic separation followed by immunoblot analysis. The log transformed measures of optical densities for each target antigen were calculated as per cent of total integrated optical densities for total protein loaded as described in the Methods section. The values for CCL4 in specific experiment were 11.8 (Control, +cells), 6.7 (AMA, +cells) and 0.7 (Control, −cells/+AMA), and for TNF were 1.6 (Control, +cells), 1.2 (AMA, +cells) and 0.8 (Control, −cells/+AMA) per cent of integrated optical densities, resepectively