- Open Access
The impact of endometrioma and laparoscopic cystectomy on serum anti-Müllerian hormone levels
- Yuh-Ming Hwu†1, 2, 3,
- Frank Shao-Ying Wu†1,
- Sheng-Hsiang Li3,
- Fang-Ju Sun3,
- Ming-Huei Lin1 and
- Robert Kuo-Kuang Lee1, 3Email author
© Hwu et al; licensee BioMed Central Ltd. 2011
Received: 8 March 2011
Accepted: 9 June 2011
Published: 9 June 2011
Serum anti-Müllerian hormone (AMH) had been proposed as a marker of ovarian reserve. The aim of this study was to evaluate the impact of endometrioma and laparoscopic cystectomy on ovarian reserve as measured by serum AMH levels.
A total of 1,642 patients were recruited in this retrospective analysis. Control group (group 1) included 1,323 infertility patients without endometrioma. Endometrioma group (group 2) included 141 patients with ovarian endometrioma. Previous cystectomy group (group 3) included 147 patients who underwent unilateral or bilateral laparoscopic cystectomy due to ovarian endometrioma more than 6 months before enrollment. Current cystectomy group (group 4) included 31 patients who underwent cystectomy during study period. Serum anti-müllerian hormone (AMH) levels were measured upon enrollment with all patients. For patients in group 4, AMH levels were measured before and 3 months after cystectomy.
Mean AMH level of patients in control group was significantly higher than that of endometrioma group or previous cystectomy group in each age subgroup, while the mean serum AMH level of the endometrioma group was also significantly higher than that of the previous cystectomy group in each age subgroup. The mean AMH level was significantly lower in patients with previous bilateral cystectomy compared to that of patients with unilateral cystectomy. The mean serum AMH level was also significantly lower in patients with bilateral endometrioma compared to that of patients with unilateral endometrioma. In group 4, mean AMH level significantly decreased from 3.95 +/- 0.42 preoperation to 2.01 +/- 0.21 ng/ml at 3-month postoperation.
Both ovarian endometrioma and cystectomy are associated with a significant reduction on ovarian reserve. Bilateral endometrioma exerts a more profound negative impact on ovarian reserve than unilateral endometrioma, regardless of either conservative or surgical intervention.
Endometrioma is one of the most commonly encountered diagnoses in ovarian surgery and may be present in up to 17-44% of patients with endometriosis [1–3]. Ovarian endometriomas are usually associated with the symptoms of dysmenorrhea, chronic pelvis pain, dyspareunia, and infertility. Previous studies have demonstrated that endometriomas can negatively affect the rate of spontaneous ovulation , as well as reducing the amount of follicular number and activity in the adjacent ovarian tissues . Based on articles review, the current 2008 ESHRE guidelines on endometriosis recommended that laparoscopic ovarian cystectomy is advised if an ovarian endometrioma ≥ 4 cm in diameter is present and hereby to confirm the diagnosis histologically, improve access to follicles, and possibly improve ovarian response [6–8]. However, some studies reported that ovarian reserve was damaged after excision of endometriomas [4, 9–11] and therefore, ESHRE guidelines also recommended that these patients should be counseled on the risk of reduced ovarian function after surgery. With the difficulties to evaluate the ovarian reserve, most of the previous studies used ovarian responses to gonadotropins as a marker to assess the ovarian reserve after endometrioma cystectomy [4, 9–11].
In the past two decades, serum anti-Müllerian hormone (AMH), day 3 FSH, E2, and inhibin B levels have been proposed as markers of ovarian reserve [12–14]. However, inhibin B, FSH, and E2 levels are all involved in the pituitary-ovary axis negative feedback  that great variations are observed during menstrual cycle. In contrast, serum AMH levels were reported to be stable throughout the menstrual cycle . La marca et al. recommended that AMH was a superior marker for predicting ovarian response over either age, FSH, or inhibin B . Therefore, AMH may also be a very informative marker regarding the degree of ovarian reserve damage due to endometrioma or ovarian cystectomy. However, very few studies have assessed the damage on ovarian reserve induced by ovarian endometrioma or cystectomy using serum AMH levels. The aim of this study was to evaluate the impact of endometrioma and laparoscopic cystectomy on ovarian reserve as measured by serum AMH levels. In addition, the differences in ovarian reserve impairment between unilateral and bilateral endometriomas and cystectomy were also investigated.
A total of 1,642 subjects were recruited in this retrospective study. Enrolled were infertility patients with or without ovarian endometrioma between the age of 22 to 46-year-old visiting MacKay Memorial Hospital between January 2007 and March 2010. The study protocol was approved by the Institutional Review Board (IRB) of Mackay Memorial Hospital in Taipei, Taiwan. Patients with subfertility problem were recruited after evaluation for infertility, including hysterosalpingography, endocrine evaluation, and ultrasonography for female patients, and semen analysis for their male partners.
Serum levels of endocrines including total testosterone, prolactin, 17-hydroxyprogesterone (17-HP), dehydroepiandrosterone sulfate (DHEAS), free thyroxine (T4), thyroid-stimulating hormone (TSH), and AMH were obtained in the early follicular phase of menstrual cycle. The exclusion criteria were as follows: patients with  polycystic ovarian syndrome according to the Rotterdam criteria ,  ovarian malignant diseases,  intake of hormonal medications within 3 months before enrollment, such as oral contraceptive pills, GnRH analogue or danazol treatment,  a body mass index (BMI) higher than 30 kg/m2,  evidence of endocrine disorders such as hyperprolactinemia, congenital adrenal hyperplasia, Cushing's syndrome, or adrenal gland tumor.
Clinical characteristics of the patients in each group
Number of patients
(Mean ± SD)
Time of serum AMH collection
34.09 ± 4.34
At time of
Endometrioma Group (Group 2)
33.27 ± 4.09
Endometrioma > 3 cm diagnosed by ultrasound and clinical diagnosis
At time of enrollment
Previous Cystectomy Group (Group 3)
33.88 ± 4.29
Previous laparoscopic cystectomy due to ovarian endometrioma
Cystectomy performed more than 6 months before enrollment
At time of enrollment
Current Cystectomy Group (Group 4)
31.14 ± 3.96
Unilateral endometrioma with laparoscopic cystectomy performed after enrollment
Unilateral cystectomy was performed after enrollment
Before cystectomy and 3 months after cystectomy
Serum AMH measurements
Serum AMH levels were measured in the early follicular phase of menstrual cycle. For groups 1, 2, and 3, serum AMH levels were obtained at outpatient department upon enrollment. For group 4, AMH levels were measured preoperatively upon enrollment and 3 months postoperatively. AMH levels were measured using a commercial enzyme-linked immunosorbent assay kit (ELISA, Diagnostic Systems Laboratories, Webster, TX) and the lowest detectable level of AMH distinguishable from zero was 0.006 ng/ml. The intra-assay and inter-assay coefficients of variation were 4.6% and 8.0% respectively.
Diagnosis of ovarian endometrioma
All recruited patients underwent transvaginal ultrasonography evaluation using a Toshiba Nemio SSA-550A System. The color and power Doppler imaging evaluation was also used upon need. According to Guerriero's study  and other previous reports, criteria for ultrasound diagnosis of endometrioma were:  cystic structure with homogenous low-level internal echoes without papillary proliferations associated with poor vascularization, and  cystic structure with homogenous low-level internal echoes with an echogenic portion in which no flow was detected [17, 18]. Follow-up ultrasound examination was performed 1-2 months aftermath to exclude spontaneously resolving hemorrhagic cyst.
Laparoscopic cystectomy techniques
All laparoscopic cystectomy operations were performed under general anesthesia during the early or middle follicular phase of menstrual cycle. The cleavage plane between the cyst wall and the normal ovarian tissue was identified after the cyst wall was incised with monopolar scissors. Then, the cyst wall was completely stripped off from the normal ovarian tissue by traction and opposite traction with two grasping forceps. Hemostasis was performed with bipolar forceps electrocoagulation. All endometrioma specimens obtained from operation were submitted for pathology examination.
Data were expressed as mean ± standard error of mean (SEM). One way analysis of variance (ANOVA) followed by the post hoc test was employed to compare the significance of differences in serum AMH levels between the control, endometrioma and cystectomy groups. The relationships between the changes in serum AMH levels and patient's age were analyzed using bivariate correlation analysis with Pearson coefficient. The relationships were presented graphically by regression curve estimation using Medcalc and SigmaPlot software.
Student's t-test was used to compare the differences in AMH levels between unilateral and bilateral endometriomas and cystectomy. Paired t-test was used to compare the differences between the sampling points (preoperative and 3 months postoperative) for the changes in AMH levels of group 4. The statistical software package SPSS version 12.0 (SPSS Inc., Chicago, IL) and SigmaPlot (Systat Software Inc., Chicago, IL) were used. All results were considered statistically significant at P < 0.05.
The effect of endometrioma and previous cystectomy on serum AMH levels
Comparison of mean serum AMH levels (ng/mL) between the groups according to age
3.94 ± 0.12
2.97 ± 0.31 b
1.74 ± 0.25 a, c
3.31 ± 0.08
2.34 ± 0.19 d
1.53 ± 0.14 a, c
1.98 ± 0.08
1.35 ± 0.19 b
0.53 ± 0.07a, c
The impact of unilateral or bilateral endometrioma on serum AMH levels
The impact of unilateral or bilateral cystectomy on serum AMH levels
The changes between the preoperative and 3-month postoperative serum AMH levels
In this study, the results demonstrated that mean serum AMH level in patients with endometrioma was significantly lower than that of patients in the control group. Additionally, the mean serum AMH level in patients with previous cystectomy was also significantly lower than patients with endometrioma (without previous cystectomy) (Figures 1 and 2) (Table 2). Based on our results, we suggest that ovarian endometrioma per se is associated with reduced ovarian reserve, and laparoscopic cystectomy can further exerted significant damage on ovarian reserve. Meanwhile, bilateral endometriomas had a more profound impact on serum AMH levels than unilateral endometriomas, regardless of either surgical cystectomy or conservative management (Figures 3, 4, 5).
The effect of endometrioma-associated damage on ovarian reserve is very difficult to assess and quantify. In the previous studies, the damaging effect of endometrioma per se on ovarian reserve was assessed by histological study , antral follicle count , ovulation rate , or responsiveness to gonadotropins as the surrogate marker [11, 20]. A meta-analysis concluded that ovarian endometrioma was associated with significantly reduced number of retrieved oocytes and developing follicles after ovarian stimulation . In our study, we used serum AMH levels to assess the ovarian reserve. The mean serum AMH level of the 141 patients with endometrioma (group 2) was significantly lower compared to the 1,323 patients without endometrioma (control group) (Figures 1, 2, Table 2). Shebl et al. reported that AMH levels were significantly lower in patients with severe endometriosis than in the control group . In agreement with Shebl's reports, we also found that patients with ovarian endometriomas had significantly lower serum AMH levels compared with patients without endometriomas. These results are compatible with those of the previous reports that showed the presence of endometrioma per se may damage the ovarian reserve measured by antral follicle count or responsiveness to gonadotropins [11, 19, 21].
To illustrate the difference in the adverse effect on ovarian reserve between unilateral and bilateral endometriomas, our results showed mean serum AMH level was significantly lower in patients with bilateral endometriomas than those with unilateral endometriomas (Figure 3). Based on the results in this study and previous reports, we suggest that the presence of endometrioma per se exerts damage on ovarian reserve. Moreover, bilateral endometriomas exert greater damage on ovarian reserve than unilateral endometriomas.
The mechanism of endometrioma inducing ovarian reserve damage is still elusive. Maneschi et al. reported that endometrioma was associated with microscopic alterations of the follicular and vascular patterns . The number of follicles was reduced and vascular network was impaired. Maneschi et al. concluded that the ovarian cortical tissue alterations could be related to either the inflammatory response to the endometriosis implants or to the toxic nature of the cystic fluid . Meanwhile, Fauvet et al. discovered an increased pro-apoptotic protein expression (bax and p21) in endometriomas compared with benign ovarian tumors . Future study is needed to elucidate the mechanisms of ovarian reserve damage induced by endometrioma.
The real amount of surgery-mediated ovarian reserve damage can not be measured directly. In the previous reports, ovarian responsiveness to gonadotropin hyperstimulation, ovarian volume, and antral follicle count (AFC) have been used as the marker for assessing ovarian reserve damage [10, 21, 24–26]. Nonetheless, AFC by transvaginal ultrasound can not be used in women without sexual experience. In the literature, ovarian responsiveness is the most often used marker of ovarian reserve. However, there is controversy about the damaging effect of cystectomy on ovarian responsiveness to hyperstimulation. Some studies have found a reduced responsiveness to hyperstimulation after cystectomy [20, 25–31] and most of them concluded that laparoscopic endometrioma cystectomy contributes to the reduction of ovarian reserve. In the current study, serum AMH level was used to replace ovarian responsiveness as the marker of ovarian reserve damage induced by cystectomy. In the previous reports, only a few studies have evaluated the ovarian reserve damage using serum AMH levels in women undergoing endometrioma cystectomy [32–34]. Tsolakidis et al. reported that the mean serum AMH level was significantly reduced 6 months after surgery . Chang et al. also observed a significant decrease in serum AMH levels 3 months after laparoscopic cystectomy . Findings of the current study are in agreement with these reports [32, 33] that it showed a significant reduction in serum AMH levels in 31 patients 3 months after cystectomy (Figure 5).
During cystectomy, it is sometimes difficult to identify and separate the cleavage plane between the cyst wall and adjacent ovarian cortex tissue due to fibrotic adhesion. Cystectomy using the stripping technique usually leads to removal of normal primary follicles and damage of ovarian reserve [35, 36]. Furthermore, bipolar coagulation at seriously bleeding sites close to ovarian hilus also leads to destruction of the ovarian blood supply and reduced ovarian reserve [24, 37]. Excessive bipolar coagulation and inadvertent removal of normal ovarian tissues adhesive to endometrioma cyst wall may contribute to lowered AMH levels after cystectomy in this study (Figure 5).
The current study also discovered that patients undergoing bilateral cystectomy for endometrioma had significantly lower AMH levels compared with patients receiving unilateral cystectomy (Figure 4). Comparable with our results, previous studies also reported that a mean number of retrieved oocytes was significantly reduced and IVF outcome was significantly impaired in patients who have undergone bilateral cystectomy than unilateral cystectomy [9, 10, 30]. Busacca et al. reported that patients who operated for bilateral endometrioma had a prevalence of 2.4% ovarian failure immediately after surgery . Similar to previous reports [9, 10], we found that two out of 144 women (1.4%) had ovarian failure 1 year after bilateral cystectomy. Another eight out of 144 women (5.4%) younger than 38 years had severe ovarian reserve damage after cystectomy. These eight women had very poor fertility outcomes and could experience ovarian failure in the near future. In the endometrioma group, we identified four women with endometriomas younger than 38 years who had very low serum AMH levels (<0.5 ng/ml). These four women may encounter a high risk of impending ovarian failure after cystectomy. To reduce such risk, it is imperative to assess the ovarian reserve before cystectomy in women with ovarian endometrioma. As women with ovarian endometrioma and a very low serum AMH level are expected to be at high risk of impending ovarian failure after cystectomy, IVF treatment before cystectomy may offer better pregnancy outcome for them.
In conclusion, the results of this study demonstrated the following observations. First, ovarian endometrioma is associated with a reduced ovarian reserve measured by serum AMH levels. Second, laparoscopic cystectomy further exerted a significant negative impact on ovarian reserve measured by serum AMH levels in both short and long term observations. Third, bilateral endometriomas had a more profound impact on ovarian reserve than unilateral endometriomas did, regardless of either conservative or surgical intervention.
For counseling of patients with ovarian endometrioma, it is imperative to identify patients with poor ovarian reserve before surgery. Assessment of ovarian reserve by serum AMH levels before cystectomy may help to prevent ovarian failure after cystectomy. The results of this study suggest that serum AMH level should be considered as a clinical routine test in patients with ovarian endometrioma before cystectomy counseling. Further large scale well-designed clinical trials are in demand to confirm this suggestion.
- Ceyhan T, Atay V, Gungor S, Karateke A, Oral O, Baser I: Efficacy of laparoscopically-assisted extracorporeal cystectomy in patients with ovarian endometrioma. J Minim Invasive Gynecol. 2006, 13: 145-149. 10.1016/j.jmig.2006.01.004.View ArticlePubMedGoogle Scholar
- Jenkins S, Olive DL, Haney AF: Endometriosis: pathogenetic implications of the anatomic distribution. Obstet Gynecol. 1986, 67: 335-338.PubMedGoogle Scholar
- Redwine DB: Ovarian endometriosis: a marker for more extensive pelvic and intestinal disease. Fertil Steril. 1999, 72: 310-315. 10.1016/S0015-0282(99)00211-3.View ArticlePubMedGoogle Scholar
- Benaglia L, Somigliana E, Vercellini P, Abbiati A, Ragni G, Fedele L: Endometriotic ovarian cysts negatively affect the rate of spontaneous ovulation. Hum Reprod. 2009, 24: 2183-2186. 10.1093/humrep/dep202.View ArticlePubMedGoogle Scholar
- Maneschi F, Marasa L, Incandela S, Mazzarese M, Zupi E: Ovarian cortex surrounding benign neoplasms: a histologic study. Am J Obstet Gynecol. 1993, 169: 388-393.View ArticlePubMedGoogle Scholar
- Kennedy S, Bergqvist A, Chapron C, D'Hooghe T, Dunselman G, Greb R, Hummelshoj L, Prentice A, Saridogan E: ESHRE guideline for the diagnosis and treatment of endometriosis. Hum Reprod. 2005, 20: 2698-2704. 10.1093/humrep/dei135.View ArticlePubMedGoogle Scholar
- Chapron C, Vercellini P, Barakat H, Vieira M, Dubuisson JB: Management of ovarian endometriomas. Hum Reprod Update. 2002, 8: 591-597. 10.1093/humupd/8.6.591.View ArticlePubMedGoogle Scholar
- Vercellini P, Chapron C, De Giorgi O, Consonni D, Frontino G, Crosignani PG: Coagulation or excision of ovarian endometriomas?. Am J Obstet Gynecol. 2003, 188: 606-610. 10.1067/mob.2003.7.View ArticlePubMedGoogle Scholar
- Busacca M, Riparini J, Somigliana E, Oggioni G, Izzo S, Vignali M, Candiani M: Postsurgical ovarian failure after laparoscopic excision of bilateral endometriomas. Am J Obstet Gynecol. 2006, 195: 421-425. 10.1016/j.ajog.2006.03.064.View ArticlePubMedGoogle Scholar
- Somigliana E, Arnoldi M, Benaglia L, Iemmello R, Nicolosi AE, Ragni G: IVF-ICSI outcome in women operated on for bilateral endometriomas. Hum Reprod. 2008, 23: 1526-1530. 10.1093/humrep/den133.View ArticlePubMedGoogle Scholar
- Somigliana E, Infantino M, Benedetti F, Arnoldi M, Calanna G, Ragni G: The presence of ovarian endometriomas is associated with a reduced responsiveness to gonadotropins. Fertil Steril. 2006, 86: 192-196. 10.1016/j.fertnstert.2005.12.034.View ArticlePubMedGoogle Scholar
- van Rooij IA, Broekmans FJ, Scheffer GJ, Looman CW, Habbema JD, de Jong FH, Fauser BJ, Themmen AP, te Velde ER: Serum antimullerian hormone levels best reflect the reproductive decline with age in normal women with proven fertility: a longitudinal study. Fertil Steril. 2005, 83: 979-987. 10.1016/j.fertnstert.2004.11.029.View ArticlePubMedGoogle Scholar
- La Marca A, Sighinolfi G, Radi D, Argento C, Baraldi E, Artenisio AC, Stabile G, Volpe A: Anti-Mullerian hormone (AMH) as a predictive marker in assisted reproductive technology (ART). Hum Reprod Update. 2010, 16: 113-130. 10.1093/humupd/dmp036.View ArticlePubMedGoogle Scholar
- La Marca A, Broekmans FJ, Volpe A, Fauser BC, Macklon NS: Anti-Mullerian hormone (AMH): what do we still need to know?. Hum Reprod. 2009, 24: 2264-2275. 10.1093/humrep/dep210.View ArticlePubMedGoogle Scholar
- Visser JA, de Jong FH, Laven JS, Themmen AP: Anti-Mullerian hormone: a new marker for ovarian function. Reproduction. 2006, 131: 1-9. 10.1530/rep.1.00529.View ArticlePubMedGoogle Scholar
- Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril. 2004, 81: 19-25.Google Scholar
- Guerriero S, Ajossa S, Mais V, Risalvato A, Lai MP, Melis GB: The diagnosis of endometriomas using colour Doppler energy imaging. Hum Reprod. 1998, 13: 1691-1695. 10.1093/humrep/13.6.1691.View ArticlePubMedGoogle Scholar
- Patel MD, Feldstein VA, Chen DC, Lipson SD, Filly RA: Endometriomas: diagnostic performance of US. Radiology. 1999, 210: 739-745.View ArticlePubMedGoogle Scholar
- Almog B, Shehata F, Sheizaf B, Tulandi T: Effect of different types of ovarian cyst on antral follicle count. Fertil Steril. 2010Google Scholar
- Suzuki T, Izumi S, Matsubayashi H, Awaji H, Yoshikata K, Makino T: Impact of ovarian endometrioma on oocytes and pregnancy outcome in in vitro fertilization. Fertil Steril. 2005, 83: 908-913. 10.1016/j.fertnstert.2004.11.028.View ArticlePubMedGoogle Scholar
- Gupta S, Agarwal A, Agarwal R, Loret de Mola JR: Impact of ovarian endometrioma on assisted reproduction outcomes. Reprod Biomed Online. 2006, 13: 349-360. 10.1016/S1472-6483(10)61439-3.View ArticlePubMedGoogle Scholar
- Shebl O, Ebner T, Sommergruber M, Sir A, Tews G: Anti muellerian hormone serum levels in women with endometriosis: a case-control study. Gynecol Endocrinol. 2009, 25: 713-716. 10.3109/09513590903159615.View ArticlePubMedGoogle Scholar
- Fauvet R, Poncelet C, Hugol D, Lavaur A, Feldmann G, Darai E: Expression of apoptosis-related proteins in endometriomas and benign and malignant ovarian tumours. Virchows Arch. 2003, 443: 38-43. 10.1007/s00428-003-0813-3.View ArticlePubMedGoogle Scholar
- Garcia-Velasco JA, Somigliana E: Management of endometriomas in women requiring IVF: to touch or not to touch. Hum Reprod. 2009, 24: 496-501.View ArticlePubMedGoogle Scholar
- Li CZ, Liu B, Wen ZQ, Sun Q: The impact of electrocoagulation on ovarian reserve after laparoscopic excision of ovarian cysts: a prospective clinical study of 191 patients. Fertil Steril. 2009, 92: 1428-1435. 10.1016/j.fertnstert.2008.08.071.View ArticlePubMedGoogle Scholar
- Ho HY, Lee RK, Hwu YM, Lin MH, Su JT, Tsai YC: Poor response of ovaries with endometrioma previously treated with cystectomy to controlled ovarian hyperstimulation. J Assist Reprod Genet. 2002, 19: 507-511. 10.1023/A:1020970417778.PubMed CentralView ArticlePubMedGoogle Scholar
- Duru NK, Dede M, Acikel CH, Keskin U, Fidan U, Baser I: Outcome of in vitro fertilization and ovarian response after endometrioma stripping at laparoscopy and laparotomy. J Reprod Med. 2007, 52: 805-809.PubMedGoogle Scholar
- Somigliana E, Ragni G, Benedetti F, Borroni R, Vegetti W, Crosignani PG: Does laparoscopic excision of endometriotic ovarian cysts significantly affect ovarian reserve? Insights from IVF cycles. Hum Reprod. 2003, 18: 2450-2453. 10.1093/humrep/deg432.View ArticlePubMedGoogle Scholar
- Benaglia L, Somigliana E, Vighi V, Ragni G, Vercellini P, Fedele L: Rate of severe ovarian damage following surgery for endometriomas. Hum Reprod. 2010, 25: 678-682. 10.1093/humrep/dep464.View ArticlePubMedGoogle Scholar
- Esinler I, Bozdag G, Aybar F, Bayar U, Yarali H: Outcome of in vitro fertilization/intracytoplasmic sperm injection after laparoscopic cystectomy for endometriomas. Fertil Steril. 2006, 85: 1730-1735. 10.1016/j.fertnstert.2005.10.076.View ArticlePubMedGoogle Scholar
- Loo TC, Lin MY, Chen SH, Chung MT, Tang HH, Lin LY, Tsai YC: Endometrioma undergoing laparoscopic ovarian cystectomy: its influence on the outcome of in vitro fertilization and embryo transfer (IVF-ET). J Assist Reprod Genet. 2005, 22: 329-333. 10.1007/s10815-005-5914-2.View ArticlePubMedGoogle Scholar
- Tsolakidis D, Pados G, Vavilis D, Athanatos D, Tsalikis T, Giannakou A, Tarlatzis BC: The impact on ovarian reserve after laparoscopic ovarian cystectomy versus three-stage management in patients with endometriomas: a prospective randomized study. Fertil Steril. 2010, 94: 71-77. 10.1016/j.fertnstert.2009.01.138.View ArticlePubMedGoogle Scholar
- Chang HJ, Han SH, Lee JR, Jee BC, Lee BI, Suh CS, Kim SH: Impact of laparoscopic cystectomy on ovarian reserve: serial changes of serum anti-Mullerian hormone levels. Fertil Steril. 2010, 94: 343-349. 10.1016/j.fertnstert.2009.02.022.View ArticlePubMedGoogle Scholar
- Ercan CM, Sakinci M, Duru NK, Alanbay I, Karasahin KE, Baser I: Antimullerian hormone levels after laparoscopic endometrioma stripping surgery. Gynecol Endocrinol. 2010, 26: 468-472. 10.3109/09513591003632134.View ArticlePubMedGoogle Scholar
- Hachisuga T, Kawarabayashi T: Histopathological analysis of laparoscopically treated ovarian endometriotic cysts with special reference to loss of follicles. Hum Reprod. 2002, 17: 432-435. 10.1093/humrep/17.2.432.View ArticlePubMedGoogle Scholar
- Muzii L, Bellati F, Bianchi A, Palaia I, Manci N, Zullo MA, Angioli R, Panici PB: Laparoscopic stripping of endometriomas: a randomized trial on different surgical techniques. Part II: pathological results. Hum Reprod. 2005, 20: 1987-1992. 10.1093/humrep/deh851.View ArticlePubMedGoogle Scholar
- Muzii L, Bellati F, Palaia I, Plotti F, Manci N, Zullo MA, Angioli R, Panici PB: Laparoscopic stripping of endometriomas: a randomized trial on different surgical techniques. Part I: clinical results. Hum Reprod. 2005, 20: 1981-1986. 10.1093/humrep/dei007.View ArticlePubMedGoogle Scholar
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