Figure 1

Phylogenetic relationship between coho salmon and other known Cx proteins. A neighbor-joining (NJ) distance tree comparing full-length amino acid sequences is shown. Bootstrap values (%) are shown for the NJ analyses. The GenBank accession numbers of the aligned deduced amino acid sequences of Cx proteins are as follows: Atlantic croaker Cx32.2 (L31542.1) and Cx32.7 (L31541.1), Atlantic salmon Cx32.2 (BT049836.1), gap junction protein alpha 1; Gja1 (BT059188.1), gap junction protein beta 1; Gjb1 (BT044946.1), gap junction protein beta 5; Gjb5 (BT057893.1) and gap junction protein beta 6; Gjb6 (NM_001140280.1), ayu Cx32.1 (DQ487733.1), Cx34.9 (DQ487730.1), Cx44.1 (DQ487731.1) and Cx44.2 (DQ487732.1), mouse, Mus musculus, Cx26 (also known as gap junction protein beta 2; Gjb2, AAA37276.1), Cx32 (also known as gap junction protein beta 1; Gjb1, AAA37296.2), Cx37 (also known as gap junction protein alpha 4; Gja4, NP_032146.1), Cx43 (also known as gap junction protein alpha 1; Gja1, NM_010288.3) and Cx45 (also known as gap junction protein gamma 1; Gjc1, NM_008122.3), rainbow trout Cx43 (also known as gap junction protein alpha 1; Gja1, DQ204869.1), red seabream Cx31.5 (AB037933.1) and Cx32.3 (AB048251.1), and zebrafish Cx28.8 (also known as gap junction protein beta 7; Gjb7, NP_001020715.1), Cx30.3 (also known as gap junction protein beta 6; Gjb6, NP_997990.2), Cx34.5 (also known as gap junction protein alpha a; Gjaa, NP_001025371.1), Cx43 (also known as gap junction protein alpha 1; Gja1, NP_571113.1), and Cx43.4 (also known as gap junction protein gamma 1; Gjc1, AAB36619.1). Human, Homo sapiens, Pannexin-1 (PANX1; NP_056183.2) was used as the outgroup.