- Open Access
Efficacy of the motile sperm organelle morphology examination (MSOME) in predicting pregnancy after intrauterine insemination
© Akl et al; licensee BioMed Central Ltd. 2011
- Received: 13 May 2011
- Accepted: 23 August 2011
- Published: 23 August 2011
Although the motile sperm organelle morphology examination (MSOME) was developed merely as a selection criterion, its application as a method for classifying sperm morphology may represent an improvement in the evaluation of semen quality. The aim of this study was to determine the prognostic value of normal sperm morphology using MSOME with regard to clinical pregnancy (CP) after intrauterine insemination (IUI).
A total of 156 IUI cycles that were performed in 111 couples were prospectively analysed. Each subject received 75 IU of recombinant FSH every second day from the third day of the cycle. Beginning on the 10th day of the cycle, follicular development was monitored by vaginal ultrasound. When one or two follicles measuring at least 17 mm were observed, recombinant hCG was administered, and IUI was performed 12-14 h and 36-40 h after hCG treatment. Prior to the IUI procedure, sperm samples were analysed by MSOME at 8400× magnification using an inverted microscope that was equipped with DIC/Nomarski differential interference contrast optics. A minimum of 200 motile spermatozoa per semen sample were evaluated, and the percentage of normal spermatozoa in each sample was determined.
Pregnancy occurred in 34 IUI cycles (CP rate per cycle: 21.8%, per patient: 30.6%). Based on the MSOME criteria, a significantly higher percentage of normal spermatozoa was found in the group of men in which the IUI cycles resulted in pregnancy (2.6+/-3.1%) compared to the group that did not achieve pregnancy (1.2+/-1.7%; P = 0.019). Logistic regression showed that the percentage of normal cells in the MSOME was a determining factor for the likelihood of clinical pregnancy (OR: 1.28; 95% CI: 1.08 to 1.51; P = 0.003). The ROC curve revealed an area under the curve of 0.63 and an optimum cut-off point of 2% of normal sperm morphology. At this cut-off threshold, using the percentage of normal sperm morphology by MSOME to predict pregnancy was 50% sensitive with a 40% positive predictive value and 79% specificity with an 85% negative predictive value. The efficacy of using the percentage of normal sperm morphology by MSOME in predicting pregnancy was 65%.
The present findings support the use of high-magnification microscopy both for selecting spermatozoa and as a routine method for analysing semen before performing IUI.
- Sperm Morphology
- Motile Spermatozoon
- Normal Sperm
- Intrauterine Insemination
- Receiver Operate Characteristic Curf Curve
Intrauterine insemination (IUI) is a simple and non-invasive procedure that is used to treat couple subfertility. In many cases, IUI allows one to avoid more complex assisted reproduction techniques (ART) such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). On the other hand, IUI yields varied results as a consequence of the large number of variables that are involved in the treatment, including the aetiology of infertility, the age of the couple, ovarian stimulation protocols, timing and number of inseminations; this has raised doubts regarding the actual effectiveness of IUI . The most common indications for IUI are cervical hostility, mild male factor, mild endometriosis, ovarian dysfunction, and unexplained infertility . In regard to male infertility, there is insufficient information regarding the effectiveness of IUI, and more data are required to either recommend or advise against IUI in these situations . Because one of the most common indications for IUI is mild male factor, and in view of the doubts regarding the efficacy of the procedure with respect to this indication, it would be of high clinical interest to determine the semen parameters that could serve as predictive factors.
Innovative methods for the selection of sperm in ART [4–9] have been published, providing fresh insight into the correlation between sperm quality and clinical results. On the other hand, the value of conventional semen analysis has been the subject of debate. By analysing semen, clinicians expect to obtain a clear indication of the male's fertilisation potential, which is not provided by conventional evaluation (except in particular situations such as total teratozoospermia and globozoospermia) [10, 11]. Although none of the semen parameters (or even the functional test)--analysed either separately or jointly--can be considered definitive, morphology has been consistently shown to be the most reliable indicator of male fertility. Diverse studies that originated primarily from IVF/ICSI programmes and IUI corroborate the sensitivity of morphology as a prognostic factor [10–20].
To test the hypothesis that subtle sperm organelle malformations [21, 22] are associated with ART results, Bartoov et al. [23, 24] developed a method of evaluating human spermatozoa in real-time at high magnification; this method is called the motile sperm organelle morphology examination (MSOME). MSOME is performed using an inverted microscope that is equipped with differential interference contrast/Nomarski optics that enable magnification exceeding 6000× , which is much higher than the magnification that is typically used by embryologists in selecting spermatozoa for the ICSI procedure (which ranges from 200× to 400×) and is even higher than that which is employed in routine semen examination (1000×). This method favoured the development of intracytoplasmic morphologically selected sperm injection (IMSI), which is based on sperm normality--as defined by the MSOME classification--and is aimed at improving conventional ICSI outcomes by focusing on the existence of a correlation between both sperm morphological abnormalities that can be observed at high magnification and DNA damage [24–28]. Although MSOME was developed merely as a selection criterion, its application as a method for classifying sperm morphology may represent an improvement in the evaluation of semen quality. In the specific case of IUI, seminal evaluation using MSOME could represent an adjunct tool to predict the efficacy of the technique.
To better comprehend the diagnostic/prognostic value of analysing semen morphology using high magnification, this study aimed to evaluate the prognostic value of normal sperm morphology using MSOME classification in predicting clinical pregnancy (CP) after IUI.
Clinical and laboratory parameters evaluated according to IUI outcome
(n = 34)
(n = 122)
Women's age (years) Mean ± SD
32.8 ± 3.9
32.3 ± 4.0
32.9 ± 3.9
Men's age (years) Mean ± SD
35.5 ± 5.6
34.9 ± 5.0
35.5 ± 5.7
Duration of infertility (years) Mean ± SD
2.8 ± 1.9
2.4 ± 1.9
2.9 ± 1.9
-Normal spermatozoa Mean ± SD
1.5 ± 2.2
2.6 ± 3.1
1.2 ± 1.2
Follicles ≥17 mm (n) Mean ± SD
1.2 ± 0.4
1.2 ± 0.4
1.2 ± 0.4
-Male (mild) + Endometriosis
DNA fragmentation (%) Mean ± SD
16.4 ± 8.0
15.0 ± 7.4
16.7 ± 8.1
Total sperm count (x106/ml) Mean ± SD
79.2 ± 54.4
83.6 ± 43.4
78 ± 57.2
Motility (% spermatozoa) (rapid + slow progression) Mean ± SD
63.2 ± 15.4
59.7 ± 14.9
64.2 ± 14.5
Leukocytes (x106) Mean ± SD
0.26 ± 0.26
0.30 ± 0.28
0.25 ± 0.26
For ovarian stimulation, each female subject received 75 IU of recombinant FSH (r-FSH; Gonal F, Serono, Brazil) every second day from the third day of the cycle. Beginning on the 10th day of the cycle, follicular development was monitored by vaginal ultrasound, and the dose of r-FSH was changed based on the ovarian response. When one or (at most) two follicles measuring at least 17 mm were observed, recombinant hCG (r-hCG; Ovidrel, Serono, Brazil) was administered.
Each patient underwent two consecutive IUI procedures (12-14 h and 36-40 h after r-hCG). Semen samples were collected into sterile containers by masturbation and immediately used for the IUI procedures. Liquefied fresh semen samples were prepared with an Isolate discontinuous concentration gradient (Irvine Scientific, Santa Ana, CA, USA). The final pellet was resuspended in 0.5 and 0.3 ml modified HTF medium (Irvine Scientific) that was supplemented with 10% human serum albumin (Irvine Scientific) for the first (12-14 h) and second (36-40 h) inseminations.
All of the IUI procedures were performed using a Frydman catheter (Frydman Classic Catheter 4.5 CCD Laboratoire CCD; Paris, France) guided by abdominal ultrasound using a 3.5 MHz convex transducer (Aloka SSD-1100; Aloka Co. Ltd., Tokyo, Japan) by the same physician. Patients with a full bladder were placed in the lithotomy position, and the cervix was exposed using a bivalve speculum. For all of the IUI procedures, the catheter passed smoothly through the cervix (without the need for uterine fixation clamps) with clear visualisation of the catheter tip upon ultrasound. In each insemination, the medium containing the spermatozoa was gently expelled into the uterine cavity under ultrasound monitoring. Following IUI, the catheter was immediately and carefully removed, and the patient was allowed to rest in bed for 15 min. Each patient received luteal phase supplementation with vaginal natural progesterone.
Pregnancy was diagnosed based on an increase in serum ß-hCG concentration 14 days after IUI. Clinical pregnancy was defined as the presence of a gestational sac that was accompanied by an image of the embryo/fetal cardiac activity on transvaginal ultrasound 4 weeks after IUI.
Determination of sperm morphology by MSOME
Before the IUI procedure, semen samples were analysed for standard semen quality parameters according to the World Health Organization  using MSOME and for DNA fragmentation using the TUNEL assay as previous described [30, 31]).
For MSOME, the liquefied fresh semen samples were prepared using the Isolate discontinuous concentration gradient (Irvine Scientific). The final pellet was resuspended in 0.2 ml modified human tubal fluid (HTF) medium (Irvine Scientific). A 1-μl aliquot of the sperm cell suspension was transferred to a 5-μl microdroplet of modified HTF medium containing 7% polyvinylpyrrolidone solution (PVP medium; Irvine Scientific). This microdroplet was placed in a sterile glass dish (FluoroDish; World Precision Instruments, USA) under sterile paraffin oil (Ovoil-100; VitroLife, Göteborg, Sweden). The sperm cells that were suspended in the microdroplet were placed on a microscope stage above an Uplan Apo ×100 oil/1.35 objective lens that was previously covered with a droplet of immersion oil. In this manner, suspended motile sperm cells in the observation droplet were then examined under high magnification using an inverted microscope (Eclipse TE 2000U; Nikon, Japan) that equipped with high-power differential interference contrast optics (DIC/Nomarski). The images were captured using a colour video camera containing effective picture elements (pixels) for high-quality image production and visualised on a colour video monitor. The morphological evaluation was performed on a monitor screen, and the combined calculated magnification was 8450× (total magnification: objective magnification = 100 ×; magnification selector = 1.0 ×; video coupler magnification = 1.0 ×; calculated video magnification = 84.50).
A spermatozoon was classified as morphologically normal when it exhibited a normal nucleus, acrosome, postacrosomal lamina, neck and tail and did not present a cytoplasm around the head . The subcellular organelles were morphologically classified as follows on the basis of the presence of specific malformations that were defined according to the arbitrary descriptive approach that was reported by Bartoov et al.  using studies utilising transmission and scanning electron microscopy: an absent, partial or vesiculated acrosome; an absent or vesiculated post-acrosomal lamina; a neck that was abaxial, disordered or showing a cytoplasmic droplet; an absent, coiled, broken, multiple or short tail.
The same technician performed all of the sperm selections. At least 200 motile spermatozoa per sample were evaluated, and the percentages of normal spermatozoa were determined. The analysis lasted 30-60 min/sample.
Data management and univariate analysis were performed using the StatsDirect statistical software (Cheshire, UK) to compare the variables between the group of cycles in which the IUI procedure resulted in pregnancy and the group in which the IUI procedure did not result in pregnancy. The analysis was performed at the cycle level (i.e., each cycle was considered as a separate unit for analysis). To compare the means of the continuous variables, the non-parametric Mann-Whitney test was used if the continuous variables were not normally distributed, and the Student's t-test was used if the continuous variables were normally distributed. The results are expressed as the arithmetic mean ± standard deviation (SD), range and median. For categorical variables, the chi-square test was used to check their association between groups, and the results are expressed as a percentage. Univariate logistic regression was also used to estimate the value of an independent variable in predicting the likelihood of becoming pregnant in an IUI cycle. The odds ratio (OR) and 95% confidence interval (CI) constituted the descriptive analysis.
Receiver operating characteristic curves (ROC) were constructed to examine the performance of the significant variables as identified by the previous statistical tests (i.e., to evaluate the ability of a variable to predict pregnancy after an IUI cycle). An optimised threshold was determined. The discriminative performance of the model was assessed by the area under the ROC curve. Sensitivity was defined as the fraction of cycles that resulted in a pregnancy that was predicted correctly, and specificity was defined as the fraction of cycles not resulting in a pregnancy that was predicted correctly. StatsDirect requires the following two columns of data for each ROC plot: one with test results in cases where the condition being tested is known to be present (pregnancy positive) and the other with test results in known negative (pregnancy negative) cases. Sensitivity is then plotted against (1-specificity). StatsDirect calculates directly the area under the ROC curve using an extended trapezoidal rule  and by a non-parametric method that is analogous to the Wilcoxon/Mann-Whitney test . A confidence interval was constructed using DeLong's variance estimate .
Pregnancy occurred in 34 IUI cycles (CP rate per cycle: 21.8%, per patient: 30.6%). A significantly higher incidence of normal spermatozoa according to the MSOME criterion was found in the group of men in which the IUI cycles resulted in pregnancy (2.6 ± 3.1%) compared to the group that did not achieve pregnancy (1.2 ± 1.7%; P = 0.019). The laboratory and clinical parameters that were evaluated according to IUI outcome are shown in Table 1. With the exception of the percentage of normal spermatozoa, there were no significant differences between the groups.
Univariate analysis of pregnancy occurrence after intrauterine insemination by logistic regression
Women's age (years)
0.87 to 1.06
Men's age (years)
0.91 to 1.05
Duration of infertility (years)
0.63 to 1.03
1.08 to 1.51
Follicles ≥17 mm (n)
0.53 to 3.68
DNA fragmentation (%)
0.97 to 1.02
Total sperm count (x106/ml)
0.99 to 1.01
Motility (% spermatozoa)(rapid + slow progression)
0.96 to 1.01
Leukocytes (x106) (mean ± SD)
0.46 to 6.70
The rationale for the IUI procedure is to maximise the number of motile spermatozoa at the site of fertilisation to maximise the likelihood of achieving pregnancy. Although IUI is used widely in the treatment of couple subfertility, its true efficacy is yet to be determined, particularly when the technique is employed due to male subfertility . According to Merviel et al. , the most important predictive factors for pregnancy in IUI cycles are the recruitment of two preovulatory follicles that are > 16 mm in a woman of age ≤30 years and a concentration ≥5 million motile spermatozoa and teratospermia ≤70% after the preparation of semen by a discontinuous concentration gradient. Another study reported that IUI that was performed for male factor subfertility has a low possibility of success when the woman's age is > 35 years, the number of motile spermatozoa that are inseminated is < 5 million, or normal sperm morphology--as defined by the criteria of the World Health Organization--is < 30% .
On the other hand, Dorjpurev et al.  showed that with a motility rate of ≥30% and a motile sperm concentration of ≥10 million/ml, IUI can be a useful tool for treating male subfertility. Kamath et al.  reported that a duration of infertility of less than 5 years and a total motile spermatozoa count of more than 10 million are related to a better prognosis in IUI, and they found a trend toward a higher pregnancy rate with endometrial thickness > 6 mm. Wainer et al.  reported that a minimum of 5 million motile spermatozoa should be inseminated only when the normal morphology of the sperm after preparation is < 30%, as the quantity compensates--at least in part--for poor seminal quality; they further noted that if these parameters cannot be reached, in vitro fertilization should be recommended. Other clinical parameters have also been evaluated, including the comparison of recombinant FSH and highly purified FSH for ovarian stimulation , the use of GnRH antagonists to avoid a premature LH surge [43, 44], the association of chromatin condensation in spermatozoa with conventional semen parameters , and the use of a soft versus firm catheter for the insemination procedure , all of which were designed to identify predictive factors of the likelihood of achieving pregnancy in IUI.
Unfortunately, MSOME is not typically applied beyond its use in sperm selection for the ICSI procedure. In fact, to the best of our knowledge, the present study is the first to analyse MSOME as a prognostic factor for IUI; thus, our results cannot be compared with other results. However, our data are in agreement with other studies that used other morphological sperm evaluation criteria. In fact, morphological sperm alterations seem to confer a significantly poorer outcome in IUI [37, 47, 48] and are reported to be a more relevant semen parameters than sperm concentration and sperm motility . Karabinus and Gelety  stated that pregnancy rates per cycle after IUI were not different when the percentage of morphologically normal sperm (strict criteria) in raw semen was 5%, 5-9%, 10-19%, 20-29%, or ≥30% (with rates of 6.55 ± 3.9,13.6 ± 3.2, 8.8 ± 2.4, 7.1 ± 2.5, and 9.7 ± 3.3%, respectively). Thus, the authors concluded that IUI appears to be a successful treatment modality for male factor infertility even in cases in which the percentage of morphologically normal sperm in raw semen is quite low.
On the other hand, the morphological criteria for assessing spermatozoa quality according to parameters of the World Health Organization were recently modified (with a normal morphology cut-off value of > 4% in the 2010 WHO manual) , demonstrating that there is still a controversy regarding the definition of a morphologically normal spermatozoon. It would be of great clinical value to establish a laboratory parameter that could reliably and more efficiently predict the outcome of IUI to both improve the outcome and optimise the indications of the technique. Recently, our group suggested that MSOME should be included among the routine criteria for semen analysis, given that it is a much stricter criterion of sperm morphology classification than the Tygerberg criterion . In addition, we also demonstrated that MSOME appears reliable for analysing semen .
One of the most important alterations that can be observed with MSOME is the presence of large nuclear vacuoles (LNV; see Figure 1B). We consider LNV as those that occupy more than 50% of the nuclear area . LNV are specific sperm alterations that can be observed at high magnification, and their presence has been shown to have clinical implications. Based on electron microscopy data, Bartoov et al. [23, 53] and Berkovitz et al.  assumed that nuclear vacuoles indicate a chromatin abnormality. Other studies confirmed the association between nuclear vacuoles at high magnification and chromatin damage. Berkovitz et al.  graded the severity of nuclear morphological alterations, highlighting principally the presence of large vacuoles and suggesting that vacuolisation of the sperm nucleus reflects some underlying chromosomal or DNA defect. Franco et al. [31, 55] demonstrated an association between large nuclear vacuoles and the presence of DNA fragmentation, denaturation and protamination in the spermatozoa. Garolla et al.  showed that the presence of nuclear vacuoles affects mitochondrial function, chromatin status, and aneuploidy rate. Using electron microscopy, Toshimori and Ito  associated the presence and content of nuclear vacuoles with DNA damage. In addition, the authors emphasised that IMSI/MSOME aids in identifying vacuoles. Oliveira et al.  observed a significant positive correlation between the percentage of sperm that contain large nuclear vacuoles and the percentage of DNA fragmentation. Gopalkrishnan et al.  found that the chromatin material of spermatozoa from men whose partners presented with early pregnancy loss was often found to be either compact or partially compact with irregular nuclear borders and large vacuoles. Moreover, a recent study has related LNV to the absence of acrosome reaction, which could have deleterious effects on the processes of fertilization and embryo development . On the other hand, it has been clearly established that the capacity of human sperm to fertilise an oocyte and produce an embryo with a high potential for implantation and development depends on the sperm cell's DNA integrity ; thus, MSOME could provide important information regarding the likelihood of success with ART.
Many investigators have reported the outcome of in vitro fertilization techniques according to MSOME [24, 25, 32, 52, 60–63], but this evaluation has not been employed previously to determine the success rate of IUI. The present study investigated the outcome of IUI compared with semen morphology as assessed with MSOME. We observed a significantly higher pregnancy rate with a higher percentage of normal cells according to the MSOME parameters. However, a logistic regression analysis failed to identify a significant correlation between treatment outcome and other characteristics such as patient age, duration of infertility, the number of follicles and other semen parameters. This is in contrast with other published reports [1, 37, 39]. The lack of consensus in the literature clearly highlights the difficulties in finding reliable prognostic parameters for determining IUI outcome.
With regard to the percentage of morphologically normal spermatozoa, we observed an optimum cut-off point of 2%. With this cut-off point, the efficacy of the exam in predicting pregnancy following IUI reached 65%, as shown by the ROC curve. Because there are doubts regarding the efficacy of IUI for male factor, MSOME could also play a role as a method for the classification of sperm morphology to offer an additional parameter for the indication of the technique. Besides the woman's age, the duration of infertility and the concentration of motile spermatozoa, which are predictive factors that are considered to be important by other investigators, our data suggest that 2% morphologically normal spermatozoa at MSOME is desirable to maximise the likelihood of pregnancy following IUI.
The accuracy with which the morphological normality of spermatozoa can be assessed depends on the resolution power of the optical magnification system. Spermatozoa that appear to be morphologically normal at 1000× magnification may in fact have various structural abnormalities that can only be detected at higher optical magnification (> 6000×). Few studies have attempted to analyse MSOME as a morphological classification method for semen. Using MSOME, Bartoov et al.  reported an incidence of sperm normality of 2.9 ± 0.5% (range 0-5%). In our study, we found an incidence of 1.5 ± 2.2% (0-13%). Differences with regard to the observation of nuclear vacuoles can explain the divergence found in the normality rates between MSOME and other morphological criteria. Employing the Tygerberg criteria, Bar-Chama et al.  analysed the number of sperm vacuoles in a series of 1,295 fresh post-processed sperm samples. They found vacuoles in only 19.5% (253) of the samples. On the other hand, MSOME revealed that the ejaculates of males who were routinely referred for ICSI exhibit an average of 30-40% of spermatozoa with vacuolated nuclei . In addition, there is divergence in the quantification (i.e., normal or abnormal) of the presence of nuclear vacuoles. Using MSOME, the nuclear chromatin content is considered to be abnormal if it contained one or more vacuoles that occupied more than 4% of the nuclear area . Nevertheless, other criteria (for example, the Tygerberg criteria) are much more tolerant with regard to the presence of vacuoles. A head is considered to be defective only when > 20% of its area is occupied by unstained vacuolar areas [29, 65].
On the other hand, Bartoov et al.  emphasised that whereas a routine morphological examination is applied to semen samples as a whole, MSOME focuses only on the fraction of motile spermatozoa. Analysing only the motile spermatozoa by MSOME can confer an additional advantage, as it will provide information regarding the sample fraction with a higher potential for achieving fertilization and development. Even though analyses using other criteria also employ high magnification, the characteristics of these procedures (i.e., fixation and staining) preclude the possibility of obtaining information for the motile portion.
In conclusion, the present study suggests that the use of MSOME for semen evaluation can be a reliable predictor of the incidence of normal forms in a sperm sample. Performing MSOME prior to the IUI procedure can represent a valuable tool to support or contraindicate this relatively inexpensive treatment and ultimately contribute to the indication of more complex ART procedures, thereby avoiding a loss of time. The present findings support the use of high-magnification microscopy both for selecting spermatozoa and as a routine method for semen analysis with potential clinical applications.
The authors wish to thank the American Journal Experts http://www.journalexperts.com for revising the English text.
- Group TECW: Intrauterine insemination. Hum Reprod Update. 2009, 15: 265-277.View ArticleGoogle Scholar
- Marchetti C, Dewailly D: Intrauterine insemination: indications and methods. Rev Prat. 2006, 56: 500-506.PubMedGoogle Scholar
- Bensdorp AJ, Cohlen BJ, Heineman MJ, Vandekerckhove P: Intra-uterine insemination for male subfertility. Cochrane Database Syst Rev. 2007, CD000360-Google Scholar
- Baccetti B: Microscopical advances in assisted reproduction. J Submicrosc Cytol Pathol. 2004, 36: 333-339.PubMedGoogle Scholar
- Gianaroli L, Magli MC, Ferraretti AP, Crippa A, Lappi M, Capitani S, Baccetti B: Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection. Fertil Steril. 2010, 93: 807-813. 10.1016/j.fertnstert.2008.10.024.View ArticlePubMedGoogle Scholar
- Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Cavagna M, Baruffi RL, Oliveira JB, Franco JG: Relationship between DNA damage and sperm head birefringence. Reprod Biomed Online. 2011, 22: 583-589. 10.1016/j.rbmo.2011.03.017.View ArticlePubMedGoogle Scholar
- Razavi SH, Nasr-Esfahani MH, Deemeh MR, Shayesteh M, Tavalaee M: Evaluation of zeta and HA-binding methods for selection of spermatozoa with normal morphology, protamine content and DNA integrity. Andrologia. 2010, 42: 13-19. 10.1111/j.1439-0272.2009.00948.x.View ArticlePubMedGoogle Scholar
- Prinosilova P, Kruger T, Sati L, Ozkavukcu S, Vigue L, Kovanci E, Huszar G: Selectivity of hyaluronic acid binding for spermatozoa with normal Tygerberg strict morphology. Reprod Biomed Online. 2009, 18: 177-183. 10.1016/S1472-6483(10)60253-2.View ArticlePubMedGoogle Scholar
- Petersen CG, Massaro FC, Mauri AL, Oliveira JB, Baruffi RL, Franco JG: Efficacy of hyaluronic acid binding assay in selecting motile spermatozoa with normal morphology at high magnification. Reprod Biol Endocrinol. 2010, 8: 149-10.1186/1477-7827-8-149.PubMed CentralView ArticlePubMedGoogle Scholar
- Ombelet W, Bosmans E, Janssen M, Cox A, Vlasselaer J, Gyselaers W, Vandeput H, Gielen J, Pollet H, Maes M, et al: Semen parameters in a fertile versus subfertile population: a need for change in the interpretation of semen testing. Hum Reprod. 1997, 12: 987-993. 10.1093/humrep/12.5.987.View ArticlePubMedGoogle Scholar
- Menkveld R, Wong WY, Lombard CJ, Wetzels AM, Thomas CM, Merkus HM, Steegers-Theunissen RP: Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds. Hum Reprod. 2001, 16: 1165-1171. 10.1093/humrep/16.6.1165.View ArticlePubMedGoogle Scholar
- Coetzee K, Kruge TF, Lombard CJ: Predictive value of normal sperm morphology: a structured literature review. Hum Reprod Update. 1998, 4: 73-82. 10.1093/humupd/4.1.73.View ArticlePubMedGoogle Scholar
- Gunalp S, Onculoglu C, Gurgan T, Kruger TF, Lombard CJ: A study of semen parameters with emphasis on sperm morphology in a fertile population: an attempt to develop clinical thresholds. Hum Reprod. 2001, 16: 110-114. 10.1093/humrep/16.1.110.View ArticlePubMedGoogle Scholar
- Kruger T, Van der Merwe F, Van Waart J: The Tygerberg strict criteria: what are the clinical thresholds for in vitro fertilization, intrauterine insemination, and in vivo fertilization?. Atlas of Human Sperm Morphology Evaluation. Edited by: Kruger T, Franken D. 2004, London: Taylor and Francis, 13-18.View ArticleGoogle Scholar
- van der Merwe FH, Kruger TF, Oehninger SC, Lombard CJ: The use of semen parameters to identify the subfertile male in the general population. Gynecol Obstet Invest. 2005, 59: 86-91. 10.1159/000082368.View ArticlePubMedGoogle Scholar
- Tasdemir I, Tasdemir M, Tavukcuoglu S, Kahraman S, Biberoglu K: Effect of abnormal sperm head morphology on the outcome of intracytoplasmic sperm injection in humans. Hum Reprod. 1997, 12: 1214-1217. 10.1093/humrep/12.6.1214.View ArticlePubMedGoogle Scholar
- De Vos A, Van De Velde H, Joris H, Verheyen G, Devroey P, Van Steirteghem A: Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil Steril. 2003, 79: 42-48. 10.1016/S0015-0282(02)04571-5.View ArticlePubMedGoogle Scholar
- Baccetti B, Capitani S, Collodel G, Estenoz M, Gambera L, Piomboni P: Infertile spermatozoa in a human carrier of robertsonian translocation 14;22. Fertil Steril. 2002, 78: 1127-1130. 10.1016/S0015-0282(02)03379-4.View ArticlePubMedGoogle Scholar
- Chemes EH, Rawe YV: Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Hum Reprod Update. 2003, 9: 405-428. 10.1093/humupd/dmg034.View ArticlePubMedGoogle Scholar
- Palermo GD, Neri QV, Takeuchi T, Rosenwaks Z: ICSI: where we have been and where we are going. Semin Reprod Med. 2009, 27: 191-201. 10.1055/s-0029-1202309.View ArticlePubMedGoogle Scholar
- Berkovitz A, Eltes F, Soffer Y, Zabludovsky N, Beyth Y, Farhi J, Levran D, Bartoov B: ART success and in vivo sperm cell selection depend on the ultramorphological status of spermatozoa. Andrologia. 1999, 31: 1-8.View ArticlePubMedGoogle Scholar
- Bartoov B, Berkovitz A, Eltes F: Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001, 345: 1067-1068. 10.1056/NEJM200110043451416.View ArticlePubMedGoogle Scholar
- Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y: Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl. 2002, 23: 1-8.PubMedGoogle Scholar
- Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, Artzi S, Gross M, Barak Y: Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003, 80: 1413-1419. 10.1016/j.fertnstert.2003.05.016.View ArticlePubMedGoogle Scholar
- Antinori M, Licata E, Dani G, Cerusico F, Versaci C, d'Angelo D, Antinori S: Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online. 2008, 16: 835-841. 10.1016/S1472-6483(10)60150-2.View ArticlePubMedGoogle Scholar
- Nadalini M, Tarozzi N, Distratis V, Scaravelli G, Borini A: Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome: a review. Reprod Biomed Online. 2009, 19 (Suppl 3): 45-55.View ArticlePubMedGoogle Scholar
- Oliveira JB, Massaro FC, Mauri AL, Petersen CG, Nicoletti AP, Baruffi RL, Franco JG: Motile sperm organelle morphology examination is stricter than Tygerberg criteria. Reprod Biomed Online. 2009, 18: 320-326. 10.1016/S1472-6483(10)60088-0.View ArticlePubMedGoogle Scholar
- Oliveira JB, Massaro FC, Baruffi RL, Mauri AL, Petersen CG, Silva LF, Vagnini LD, Franco JG: Correlation between semen analysis by motile sperm organelle morphology examination and sperm DNA damage. Fertil Steril. 2010, 94: 1937-1940. 10.1016/j.fertnstert.2010.01.042.View ArticlePubMedGoogle Scholar
- World Health Organization: WHO laboratory manual for the examination and processing of human semen. 2010, Geneva: World Health Organization, 5Google Scholar
- Vagnini L, Baruffi RL, Mauri AL, Petersen CG, Massaro FC, Pontes A, Oliveira JB, Franco JG: The effects of male age on sperm DNA damage in an infertile population. Reproductive biomedicine online. 2007, 15: 514-519. 10.1016/S1472-6483(10)60382-3.View ArticlePubMedGoogle Scholar
- Franco JG, Baruffi RL, Mauri AL, Petersen CG, Oliveira JB, Vagnini L: Significance of large nuclear vacuoles in human spermatozoa: implications for ICSI. Reprod Biomed Online. 2008, 17: 42-45. 10.1016/S1472-6483(10)60291-X.View ArticlePubMedGoogle Scholar
- Berkovitz A, Eltes F, Yaari S, Katz N, Barr I, Fishman A, Bartoov B: The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod. 2005, 20: 185-190.View ArticlePubMedGoogle Scholar
- ASTM: Standard E1951-02 Physical and Mechanical Testing Standards West Conshohocken, PA. [http://www.astm.org]
- Press WH: Numerical recipes in FORTRAN: the art of scientific computing. 1992, Cambridge England; New York, NY, USA: Cambridge University Press, 2Google Scholar
- Hanley JA, McNeil BJ: The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology. 1982, 143: 29-36.View ArticlePubMedGoogle Scholar
- DeLong ER, DeLong DM, Clarke-Pearson DL: Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics. 1988, 44: 837-845. 10.2307/2531595.View ArticlePubMedGoogle Scholar
- Merviel P, Heraud MH, Grenier N, Lourdel E, Sanguinet P, Copin H: Predictive factors for pregnancy after intrauterine insemination (IUI): an analysis of 1038 cycles and a review of the literature. Fertil Steril. 2010, 93: 79-88. 10.1016/j.fertnstert.2008.09.058.View ArticlePubMedGoogle Scholar
- Badawy A, Elnashar A, Eltotongy M: Effect of sperm morphology and number on success of intrauterine insemination. Fertil Steril. 2009, 91: 777-781. 10.1016/j.fertnstert.2007.12.010.View ArticlePubMedGoogle Scholar
- Dorjpurev U, Kuwahara A, Yano Y, Taniguchi T, Yamamoto Y, Suto A, Tanaka Y, Matsuzaki T, Yasui T, Irahara M: Effect of semen characteristics on pregnancy rate following intrauterine insemination. J Med Invest. 2011, 58: 127-133. 10.2152/jmi.58.127.View ArticlePubMedGoogle Scholar
- Kamath MS, Bhave P, Aleyamma T, Nair R, Chandy A, Mangalaraj AM, Muthukumar K, George K: Predictive factors for pregnancy after intrauterine insemination: A prospective study of factors affecting outcome. J Hum Reprod Sci. 2010, 3: 129-134. 10.4103/0974-1208.74154.PubMed CentralView ArticlePubMedGoogle Scholar
- Wainer R, Albert M, Dorion A, Bailly M, Bergere M, Lombroso R, Gombault M, Selva J: Influence of the number of motile spermatozoa inseminated and of their morphology on the success of intrauterine insemination. Hum Reprod. 2004, 19: 2060-2065. 10.1093/humrep/deh390.View ArticlePubMedGoogle Scholar
- Matorras R, Osuna C, Exposito A, Crisol L, Pijoan JI: Recombinant FSH versus highly purified FSH in intrauterine insemination: systematic review and metaanalysis. Fertil Steril. 2011, 95: 1937-1942. 10.1016/j.fertnstert.2011.02.030. e1933View ArticlePubMedGoogle Scholar
- Allegra A, Marino A, Coffaro F, Scaglione P, Sammartano F, Rizza G, Volpes A: GnRH antagonist-induced inhibition of the premature LH surge increases pregnancy rates in IUI-stimulated cycles. A prospective randomized trial. Hum Reprod. 2007, 22: 101-108.View ArticlePubMedGoogle Scholar
- Cantineau AE, Cohlen BJ, Klip H, Heineman MJ: The addition of GnRH antagonists in intrauterine insemination cycles with mild ovarian hyperstimulation does not increase live birth rates--a randomized, double-blinded, placebo-controlled trial. Hum Reprod. 2011, 26: 1104-1111. 10.1093/humrep/der033.View ArticlePubMedGoogle Scholar
- Lazaros L, Kaponis A, Vartholomatos G, Hatzi E, Botsari S, Plachouras N, Makrydimas G, Zikopoulos K, Sofikitis N, Georgiou I: Using semen flow cytometry to evaluate association of ploidy status and chromatin condensation of spermatozoa with conventional semen parameters: clinical application in intrauterine insemination. Fertil Steril. 2011, 95: 110-115. 10.1016/j.fertnstert.2010.05.012.View ArticlePubMedGoogle Scholar
- van der Poel N, Farquhar C, Abou-Setta AM, Benschop L, Heineman MJ: Soft versus firm catheters for intrauterine insemination. Cochrane Database Syst Rev. 2010, CD006225-Google Scholar
- Burr RW, Siegberg R, Flaherty SP, Wang XJ, Matthews CD: The influence of sperm morphology and the number of motile sperm inseminated on the outcome of intrauterine insemination combined with mild ovarian stimulation. Fertil Steril. 1996, 65: 127-132.PubMedGoogle Scholar
- Belaisch-Allart J, Mayenga JM, Plachot M: [Intra-uterine insemination]. Contracept Fertil Sex. 1999, 27: 614-619.PubMedGoogle Scholar
- Guerif F: [Which assisted reproduction technique as a function of sperm morphology?]. Gynecol Obstet Fertil. 2010, 38: 508-510. 10.1016/j.gyobfe.2010.07.002.View ArticlePubMedGoogle Scholar
- Karabinus DS, Gelety TJ: The impact of sperm morphology evaluated by strict criteria on intrauterine insemination success. Fertil Steril. 1997, 67: 536-541. 10.1016/S0015-0282(97)80082-9.View ArticlePubMedGoogle Scholar
- Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, Haugen TB, Kruger T, Wang C, Mbizvo MT, Vogelsong KM: World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010, 16: 231-245. 10.1093/humupd/dmp048.View ArticlePubMedGoogle Scholar
- Oliveira JB, Petersen CG, Massaro FC, Baruffi RL, Mauri AL, Silva LF, Ricci J, Franco JG: Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles. Reprod Biol Endocrinol. 2010, 8: 56-10.1186/1477-7827-8-56.PubMed CentralView ArticlePubMedGoogle Scholar
- Bartoov B, Fisher J, Eltes F, Langzam J, Lunenfeld B: A comparative morphological analysis of abnormal human spermatozoa. Advances in Diagnosis and Treatment of Infertility. Edited by: Insler V, Bettendorf G. 1981, Amsterdam: Elsevier/North Holland, 355-373.Google Scholar
- Berkovitz A, Eltes F, Ellenbogen A, Peer S, Feldberg D, Bartoov B: Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome?. Hum Reprod. 2006, 21: 1787-1790. 10.1093/humrep/del049.View ArticlePubMedGoogle Scholar
- Franco JG, Mauri AL, Petersen CG, Massaro FC, Silva LF, Felipe V, Cavagna M, Pontes A, Baruffi RL, Oliveira JB, Vagnini LD: Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Andro. 2011Google Scholar
- Garolla A, Fortini D, Menegazzo M, De Toni L, Nicoletti V, Moretti A, Selice R, Engl B, Foresta C: High-power microscopy for selecting spermatozoa for ICSI by physiological status. Reprod Biomed Online. 2008, 17: 610-616. 10.1016/S1472-6483(10)60307-0.View ArticlePubMedGoogle Scholar
- Toshimori K, Ito C: Human sperm ultrastructures and fertility. J Mamm Ova Res. 2008, 25: 232-239. 10.1274/0916-7625-25.4.232.View ArticleGoogle Scholar
- Gopalkrishnan K, Padwal V, Meherji PK, Gokral JS, Shah R, Juneja HS: Poor quality of sperm as it affects repeated early pregnancy loss. Arch Androl. 2000, 45: 111-117. 10.1080/014850100418800.View ArticlePubMedGoogle Scholar
- Kacem O, Sifer C, Barraud-Lange V, Ducot B, De Ziegler D, Poirot C, Wolf J: Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin. Reprod Biomed Online. 2010, 20: 132-137. 10.1016/j.rbmo.2009.10.014.View ArticlePubMedGoogle Scholar
- Junca A, Cohen-Bacrie P, Hazout A: Improvement of fertilization and pregnancy rate after intracytoplasmic fine morphologically selected sperm injection. Fertil Steril. 2004, 82: S173-View ArticleGoogle Scholar
- Hazout A, Dumont-Hassan M, Junca AM, Cohen Bacrie P, Tesarik J: High-magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online. 2006, 12: 19-25. 10.1016/S1472-6483(10)60975-3.View ArticlePubMedGoogle Scholar
- Vanderzwalmen P, Hiemer A, Rubner P, Bach M, Neyer A, Stecher A, Uher P, Zintz M, Lejeune B, Vanderzwalmen S, et al: Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online. 2008, 17: 617-627. 10.1016/S1472-6483(10)60308-2.View ArticlePubMedGoogle Scholar
- Tasaka A, Doshida M, Sato Y, Kyoya T, Nakajo Y, Kyono K: Outcome of IMSI (intracytoplasmic morphologically selected sperm injection) in patients with repeated ICSI failures. Fertil Steril. 2009, 92: S76-View ArticleGoogle Scholar
- Bar-Chama N, Schiff J, Luna M, Dann AB, Copperman JB: The level of sperm vacuoles in the fresh post-processed sperm sample significantly affects IVF cycle outcomes. Fertil Steril. 2007, 88: S18-View ArticleGoogle Scholar
- Franken DR, Kruger TF: The what is a normal spermatozoon?. Atlas of Human Sperm Morphology Evaluation. Edited by: Kruger TF, Franken DR. 2004, London: Taylor and Francis, 49-74.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.