in situ hybridization analysis of Tnp2 expression during recovery after irradiation. ISH analysis of Tnp2 expression during recovery in adult mouse testis after irradiation with 1 Gy. A control, pi days 14, 24 and 38 were shown. Low magnification images of whole testis are shown (left) together with a higher magnification of a representative part of the testis (right). The bars correspond to 100 μm. ISH from additional pi days can be found in Additional file 3.