- Open Access
In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with Escherichia coli in human fetal membranes
© Zaga-Clavellina et al; licensee BioMed Central Ltd. 2007
- Received: 17 August 2007
- Accepted: 13 December 2007
- Published: 13 December 2007
Chorioamniotic membranes infection is a pathologic condition in which an abnormal secretion of proinflammatory cytokines halts fetal immune tolerance. The aim of the present study was to evaluate the functional response of human chorioamniotic membranes, as well as the individual contribution of the amnion and choriodecidua after stimulation with Escherichia coli, a pathogen associated with preterm labor.
Explants of chorioamniotic membranes from 10 women (37–40 weeks of gestation) were mounted and cultured in a Transwell system, which allowed us to test the amnion and choriodecidua compartments independently. Escherichia coli (1 × 10 6 CFU/mL) was added to either the amniotic or the choriodecidual regions or both; after a 24-h incubation, the secretion of IL-1beta, IL-6, TNFalpha, IL-8, and IL-10 in both compartments was measured using a specific ELISA. Data were analyzed by Kruskal-Wallis one-way analysis of variance.
After stimulation with Escherichia coli, the choriodecidua compartment showed an increase in the secretion of IL-1beta (21-fold), IL-6 (2-fold), IL-8 (6-fold), and IL-10 (37-fold), regardless of which side of the membrane was stimulated; TNFalpha secretion augmented (22-fold) also but only when the stimulus was applied simultaneously to both sides. When the amnion was stimulated directly, the level of IL-1beta (13-fold) rose significantly; however, the increase in IL-8 secretion was larger (20-fold), regardless of the primary site of infection. TNFalpha secretion in the amnion compartment rose markedly only when Escherichia coli was simultaneously applied to both sides.
Selective stimulation of fetal membranes with Escherichia coli results in a differential production of IL-1beta, IL-6, TNFalpha, IL-8, and IL-10. These tissues were less responsive when the amnion side was stimulated. This is in agreement with the hypothesis that the choriodecidua may play a primary role during an ascending intrauterine infection, being the main barrier to progression of the infection into the amniotic cavity. Therefore, the tissue-specific capacities for the secretion of these immune modulators could be a determining factor for the degree of severity of the inflammation process in fetal membranes.
- Amniotic Fluid
- Preterm Labor
- Fetal Membrane
- Intrauterine Infection
Pregnancy is the result of a fine immunological privilege that allows the fetus to co-habit the maternal uterus, preventing rejection of the fetal allograft . There are epidemiologic and experimental data indicating that intrauterine infection is a pathological condition that disrupts this privilege [2, 3] and is the main etiological factor for the development of Premature Rupture of Membranes (PROM). This pathology complicates about 30–40% of all preterm births  and 10% of all pregnancies .
Human chorioamniotic membranes form a complex multi-laminated tissue constituted by the amnion whose epithelium is in contact with the amniotic fluid and the choriodecidua that is formed by trophoblasts inter-digitized with the maternal decidua [5, 6]. During an ascending infection, originated in the cervico-vaginal region [7–9], the choriodecidua is the first-line barrier in contact with the pathogens that can cross the membranes and infect the amnion and the amniotic fluid . In response, there is an abnormal production of proinflammatory cytokines, such as interleukin (IL)-1β, tumor necrosis factor alpha (TNFα), IL-6, and IL-8 in the extra-embryonic tissues, i.e., placenta  and chorioamniotic membranes [11–14].
In a previous work, using an ex-vivo model, we demonstrated that in vitro stimulation with lipopolysaccharide (LPS) and Streptococcus agalactiae induced a differential response in IL-1β and TNFα secretion by the amnion and choriodecidual tissues . Using the same model, the present study was aimed at: 1) Investigating the secretory profiles of IL-1β, IL-6, TNFα, IL-8, and IL-10 by human chorioamniotic membranes after stimulation with Escherichia coli, a common pathogen in cervical-vaginal infection in humans associated with pregnancy losses [8, 15], fetal cardiac dysfunction , neurological injury in preterm infants , as well as with preterm delivery and PROM . 2) Disclosing whether or not a specific differential contribution by each membrane exists.
The 10 pregnant women (37–40 weeks of gestation) studied were from an urban area of Mexico City, 22–35 years old, previously normotensive, without history of diabetes mellitus, thyroid, liver or chronic renal disease, cared for at the Obstetrics Outpatient Service of the Instituto Nacional de Perinatologia. All women had uneventful pregnancies, without evidence of active labor and with neither clinical nor microbiological signs of chorioamnionitis or of lower genital tract infection. Samples were obtained after delivery by elective cesarean section.
All women provided written, informed consent, before collection of samples. The institutional Review Board approved the protocol and the collection and use of the samples and the study was conducted according to the Guidelines on the Practice of Ethical Committees in Medical Research (3rd ed) issued by the Royal College of Physicians of London.
Fetal membrane explants culture
To stabilize the explants after manipulation, they were pre-incubated for 24 h in the medium (DMEM-FCS). Subsequently, the explants were washed with saline solution to remove FCS and the remainder of the medium was changed to DMEM with 0.2% lactalbumin hydrolysate (Gibco BRL) and co-incubated with 1 × 106 colony forming units (CFU) of Escherichia coli isolated from cervicovaginal exudates.
Four Each experiment (n = 10) included the following set of chambers, in triplicate: Basal (n = 10), control membranes in which only the medium culture was added to the compartments; Choriodecidua (n = 10), Escherichia coli was added only to the choriodecidua side; Amnion (n = 10), E. coli was added only to the amniotic compartment; Both (n = 10), the bacterium was added simultaneously to both compartments. After 24 h of co-incubation, the medium from the amnion and choriodecidua chambers was collected and centrifuged at 5,000 rpm, 3 min at 4°C, to precipitate Escherichia coli, and the bacterium-free medium of each sample was stored at -70°C until assayed. Protein concentration in all samples was measured according to the Bradford method .
IL-1β, TNF-α, IL-6, and IL-10 concentrations were quantified using specific DuoSet® enzyme-linked immunosorbent sandwich assays (ELISA) (R & D Systems, Minneapolis, USA). For the IL-1β assay, a standard curve was developed from 4 to 260 pg/mL and the sensitivity was 2 pg/mL; the TNF-α assay was linear from 15 to 960 pg/mL and sensitivity was 5.0 pg/mL; for the IL-6 assay, the curve was linear from 250 to 8000 pg/mL with a sensitivity of 200 pg/mL; and for the IL-10 assay, a standard curve was developed from 1.25 to 2000 pg/mL, with a sensitivity of 1.0 pg/mL. IL-8 was measured using a commercial kit (Amersham Biosciences, Buckinghamshire, UK) according to manufacturer's instructions. The standard curve was linear from 25.6 to 1000 pg/mL and sensitivity was 5 pg/mL.
The final concentration of each cytokine was expressed per microgram of the total protein concentration of each sample. Intra- and inter-assay coefficients of variation were less than 5%. A rigorous quality control program, including external and internal standards for all cytokines, is followed in our laboratory.
Since the results did not have a normal distribution, comparisons between the experimental groups and the control were performed using the Kruskal-Wallis one-way analysis of variance on rank tests. A P < 0.05 was considered significant. The data expressed in the text and figures represent the median and the 95% confidence interval limits (95% CI).
In the present study we used a previously published ex-vivo model that resembles the presence of two independent compartments with a fully functional chorioamnion; its upper chamber is delimited by the choriodecidua and the lower chamber by the amnion, allowing us to test both compartments simultaneously . We included three different conditions of membrane stimulation with Escherichia coli, resembling clinical scenarios. 1) Microorganisms arrive to the choriodecidua through ascendant colonization (choriodecidua stimulation), 2) bacteria reach the amnion side by iatrogenic introduction during amniocentesis (amnion stimulation), and 3) bacteria are in contact with both sides of the membranes at late stages of colonization, which enables them to cross the membranes.
With this system, we were able to show that the basal secretions of IL-1β, IL-6, IL-10, and IL-8 by the choriodecidual tissue increased significantly (P < 0.05) in response to Escherichia coli stimulation, regardless of whether the stimulus was applied directly to this region, the amnion, or to both regions simultaneously. In addition, TNFα increased markedly in both the choriodecidua and the amnion sides when the bacterium was applied to both membranes at the same time. IL-1β rose mildly when the amnion was directly stimulated by Escherichia coli whereas IL-8 secretion rose markedly in the amnion regardless of the primary side stimulated. A marked rise in TNFα secretion in the amnion side was also observed but only when the bacterium was simultaneously applied to the amnion and choriodecidua tissues.
Previous works have evidenced that inoculation of the amniotic cavity with Escherichia coli induces a toxic response, characterized by the overproduction of pro-inflammatory cytokines [20, 21], such as IL-1β, a potent immunomodulator able to induce preterm labor after its experimental infusion in pregnant rhesus monkeys . However, the used animal models do not allow characterizing the particular and specific contribution of each of the chorioamniotic membranes in this response and cannot be compared with our results. In addition, we demonstrated that in vitro stimulation of chorioamniotic membranes with lipopolysaccharide (LPS) and Streptococcus agalactiae induces IL-1β increase in the choriodecidua region .
On the other hand, the IL-6 concentration in the amniotic fluid is considered a marker of intra-amniotic inflammation and is frequently associated with an infectious process in either the amniotic fluid or the chorioamniotic space [23, 24]. Previous evidence has demonstrated that the concentration of this cytokine is increased in human decidua  and chorion cells  after treatment with IL-1β and TNFα.
Similar findings have been obtained with IL-10, an anti-inflammatory cytokine, to which a role as therapeutic factor in preterm labor has been ascribed  and whose concentration increases in patients with preterm labor associated with intrauterine infection .
The differential secretion capacities of pro-inflammatory cytokines by the amnion and the choriodecidua after Escherichia coli stimulation here reported suggest the existence of a complex interactive regulation network. There is evidence indicating that IL-1β , TNFα , IL-6 , and IL-10 , in different gestational tissues, are able to induce biosynthesis and secretion of prostaglandin E2 (PGE2) and PGF2α; these uterotonic factors play key roles in the onset and progression of labor  in normal and pathological conditions. In a previous work, we demonstrated that the amnion's epithelium is the main source of PGE2 secretion after stimulation with Candida albicans, yeast that has been associated with cervico-vaginal infections .
Under the experimental conditions used in our study, it was clear that IL-1β, IL-6, and IL-10 were mainly secreted to the choriodecidual compartment regardless of the primary zone stimulated with Escherichia coli. Interestingly, when the amnion was primarily stimulated, its contribution to increase the secretion of theses cytokines was small if any; whereas the secretion of theses cytokines by the choriodecidual region was significantly increased. This observation suggests the existence of a communication or "cross-talk" between both regions and, thus, it is possible that the amnion's epithelium might be an important indirect factor in the whole cytokine response.
On the other hand, TNFα, whose negative effects on the course of pregnancy have been characterized [34, 35], increases in the amniotic fluid of women with preterm labor and intra-amniotic infection . There is also experimental evidence demonstrating that the administration of a TNFα bolus to pregnant animals causes profuse hemorrhage and pregnancy termination [36, 37]. In our model, the increase in TNFα secretion in both the amnion and choriodecidua compartments was significant (P < 0.05) only after simultaneous stimulation of both membrane sides. A possible interpretation of the biological significance of these findings is that, since TNFα is a pro-inflammatory cytokine with major immuno-toxic properties, the chorioamniotic membrane coordinates its secretion in response to a very complicated infectious scenario, as represented by chorioamnionitis, in which both the fetal and maternal sides are insulted by an infectious agent.
It is possible that the secretion of IL-1β, IL-6, IL-10, and TNFα in the choriodecidua region after infection with Escherichia coli would favor their trans-membranal translocation to the amnion  and thereby exert their effect on the whole membrane.
The IL-8 secretion pattern was also interesting, because both the amnion and the choriodecidua were active in the secretion of this chemokine. This ubiquitous production might play a key role in the recruitment and activation of professional cells of the immune system, such as neutrophils whose migration toward the cervix, the placenta, and chorioamniotic membranes is a clinical/histological characteristic of infection/inflammation [39, 40]. The present results show that the IL-8 response in the amnion was mild as compared to the choriodecidua region, which is the first tissue to be colonized by the microbial pathogen during an ascending intrauterine infection and is the main barrier to progression of infection into the amniotic cavity. Therefore, the tissue-specific capacities could be important factors in determining the severity of the inflammation in fetal membranes infected with Escherichia coli.
It is tempting to hypothesize that Escherichia coli infection of the chorioamniotic membrane may induce a precocious onset of the overproduction of pro-inflammatory cytokines (an "anticipation" of the normal parturition cascade?), leading to PROM and preterm labor.
Our results demonstrated that fetal membranes respond differentially to Escherichia coli infection. The amnion and choriodecidual cellular populations display a cooperative and bidirectional communication to secret different immunologic modulators, such as Il-1β, TNFα, IL-6, IL-8, and IL-10.
The choriodecidua is the most responsive region to the infection, as it is the first tissue to be colonized by the microbial pathogen during an ascending intrauterine infection and it is the main barrier to progression of the infection into the amniotic cavity. Therefore, the tissue-specific capacities of this region to secrete different proinflammatory cytokines are crucial factors for determining the severity of the inflammation process of fetal membranes.
This work was supported by the National Council of Science and Technology of Mexico (CONACyT, Grant No. 61590). We thank Dr. Adalberto Parra for his critical suggestions to the manuscript.
- Thellin O, Coumans B, Zorzi W, Igout A, Heinen E: Tolerance to the foeto-placental "graft": ten ways to support a child for nine months. Curr Opin Immunol. 2000, 12: 731-737. 10.1016/S0952-7915(00)00170-9.View ArticlePubMedGoogle Scholar
- Peltier MR: Immunology of term and preterm labor. Reprod Biol Endocrinol. 2003, 1: 122-132. 10.1186/1477-7827-1-122.PubMed CentralView ArticlePubMedGoogle Scholar
- Makhseed M, Raghupathy R, El-Shazly S, Azizieh F, Al-Harmi JA, Al-Azemi MMK: Pro-inflammmatory maternal cytokines profile in preterm delivery. Am J Reprod Immunol. 2003, 49: 308-318. 10.1034/j.1600-0897.2003.00038.x.View ArticlePubMedGoogle Scholar
- Newton ER: Preterm labor, preterm premature rupture of membranes and chorioamnionitis. Clin Perinatol. 2005, 32: 571-600. 10.1016/j.clp.2005.05.001.View ArticlePubMedGoogle Scholar
- Parry S, Strauss JF: Premature rupture of the fetal membranes. N Engl J Med. 1998, 338: 663-668. 10.1056/NEJM199803053381006.View ArticlePubMedGoogle Scholar
- Malak TM, Ockleford CD, Bell SC, Dalgleish R, Bright N, Macvicar J: Confocal immunofluorescence localization of collagen types I, III, IV, V and VI and their ultraestructural organization in term human fetal membranes. Placenta. 1993, 14: 385-406. 10.1016/S0143-4004(05)80460-6.View ArticlePubMedGoogle Scholar
- Romero R, Espinoza J, Goncalves LF, Kusanovic JP, Fiel LA, Nien JK: Inflammation in preterm and term labour and delivery. Sem Fetal Neonatal Med. 2006, 11: 317-326. 10.1016/j.siny.2006.05.001.View ArticleGoogle Scholar
- Moyo SR, Tswana SA, Nystrom L, Bergstrom S, Blomerberg J, Ljungh A: Intrauterine death and infections during pregnancy. Int J Gynaecol Obstet. 1995, 51: 211-218. 10.1016/0020-7292(95)80004-2.View ArticlePubMedGoogle Scholar
- El-Shazly S, Machseed M, Azizieh F, Raghupathy R: Increased expression of pro-inflammatory cytokines in placentas of women undergoing spontaneous preterm delivery or premature rupture of membranes. Am J Reprod Immunol. 2004, 52: 45-52. 10.1111/j.1600-0897.2004.00181.x.View ArticlePubMedGoogle Scholar
- Romero R, Mazor M: Infection and preterm labor. Clin Obstet Gynecol. 1988, 31: 553-584. 10.1097/00003081-198809000-00006.View ArticlePubMedGoogle Scholar
- Schoonmaker JN, Lawellin DW, Lunt B, McGregor JA: Bacteria and inflammatory cells reduce chorioamniotic membrane integrity and tensile strength. Obstet Gynecol. 1989, 74: 590-596.PubMedGoogle Scholar
- Tashima LS, Millar LK, Bryant-Greenwood GD: Genes upregulated in human fetal membranes by infection or labor. Obstet Gynecol. 1999, 94: 441-449. 10.1016/S0029-7844(99)00339-7.View ArticlePubMedGoogle Scholar
- Zaga V, Estrada-Gutierrez G, Beltran-Montoya J, Maida-Claros R, Lopez-Vancell R, Vadillo-Ortega F: Secretion of interleukin-1 beta and tumor necrosis factor alpha by whole fetal membranes depends on initial interactions of amnion or chorion with lipopolysaccharides or group B streptococci. Biol Reprod. 2004, 71: 1296-1302. 10.1095/biolreprod.104.028621.View ArticlePubMedGoogle Scholar
- Zaga-Clavellina V, García-López G, Estrada-Gutierrrez G, Martínez-Flores A, Maida-Claros R, Beltrán-Montoya J, Vadillo-Ortega F: Incubation of human chorioamniotic membranes with Candida albicans induces differential synthesis and secretion of interleukin-1β, inteleukin-6, prostaglandin E2, and 92 kDa type IV collagenase. Mycoses. 2005, 48: 1-8.Google Scholar
- Deb k, Chaturvedi MM, Jaiswal YK: Comprehending the role of LPS in Gram-negative bacterial vaginosis: ogling into the causes of unfulfilled child-wish. Arch Gynecol Obstet. 2004, 270: 133-146. 10.1007/s00404-004-0623-0.View ArticlePubMedGoogle Scholar
- Rounioja S, Rasanen J, Glumoff V, Ojaniemi M, Makikallio K, Hallman M: Intra-amniotic lipopolysaccharide leads to fetal cardiac dysfunction. A mouse model for fetal inflammatory response. Cardiovasc Res. 2003, 60: 156-164. 10.1016/S0008-6363(03)00338-9.View ArticlePubMedGoogle Scholar
- Dunkan JR, Cock ML, Scheerlinck JP, Westcott KT, McLean C, Harding R, Rees SM: White matter injury after repeated endotoxin exposure in the preterm ovine fetus. Pediatr Res. 2002, 52: 941-949.View ArticleGoogle Scholar
- Romero R, Mazor M: Infection and preterm labor. Clin Obstet Gynecol. 1988, 31: 553-584. 10.1097/00003081-198809000-00006.View ArticlePubMedGoogle Scholar
- Stoscheck CM: Quantitation of Protein. Methods in Enzymology. 1990, 182: 50-69.View ArticlePubMedGoogle Scholar
- Hirsch E, Saotomo I, Hirsh D: A model of intraturerine infection and preterm delivery in mice. Am J Obstet Gynecol. 1995, 172: 1598-1603. 10.1016/0002-9378(95)90503-0.View ArticlePubMedGoogle Scholar
- Reznikov LL, Fantuzzi G, Selzman CH, Shames BD, Barton HA, Bell H, McGregor JA, Dinarello CA: Utilization of endoscopic inoculation in a mouse model of intrauterine infection-induced preterm birth; role of interleukin 1β. Biol Reprod. 1999, 60: 1232-1238. 10.1095/biolreprod60.5.1231.View ArticleGoogle Scholar
- Vadillo-Ortega F, Sadowsky DW, Haluska GJ, Hernández-Guerrero C, Guevara-Silva Rebeca, Gravett MG, Novy MJ: Identification of matrix metalloproteinase-9 in amniotic fluid and amniochorion in spontaneous labor and after experimental intrauterine infection or interleukin-1β infusion in pregnant rhesus monkeys. Am J Obstet Gynecol. 2002, 186: 128-138. 10.1067/mob.2002.118916.View ArticlePubMedGoogle Scholar
- Romero R, Avila C, Santhanam U, Sehgal PB: Amniotic fluid interleukin-6 in preterm labor: Association with labor. J Clin Invest. 1990, 85: 1392-1400.PubMed CentralView ArticlePubMedGoogle Scholar
- Hsu CD, Meaddough E, Aversa K, Hong SF, Lu LC, Jones DC, Copel JA: Elevated amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 in intra-amniotic infection. Am J Obstet Gynecol. 1998, 179: 1267-1270. 10.1016/S0002-9378(98)70144-9.View ArticlePubMedGoogle Scholar
- Dudley DJ, Trautman MS, Araneo BA, Edwin SS, Mitchell MD: Decidual cell biosynthesis of interleukin-6: regulation by inflammatory cytokines. J Clin Endocrinol Metab. 1992, 74: 884-889. 10.1210/jc.74.4.884.View ArticlePubMedGoogle Scholar
- Dudley DJ, Trautman MS, Edwin SS, Ludin-Schiller S, Mitchel MD: Biosynthesis of interleukin-6 by cultured human chorion laeve cells: regulation by cytokines. J Clin Endocrinol Metab. 1992, 75: 1081-1086. 10.1210/jc.75.4.1081.PubMedGoogle Scholar
- Sato TA, Keelan JA, Mitchell MD: Critical paracrine interactions between TNFα and IL-10 regulate lipopolysaccharide-stimulated human choriodecidual cytokine and prostaglandin E2 production. J Immnunol. 2003, 170: 158-166.View ArticleGoogle Scholar
- Grieg PC, Herbert WNP, Robinnette BL, Teot LA: Amniotic fluid interleukin-10 concentrations increase through pregnancy and are elevated in patients with preterm labor associated with intrauterine infection. Am J Obstet Gynecol. 1995, 173: 1223-1227. 10.1016/0002-9378(95)91358-0.View ArticleGoogle Scholar
- Romero R, Brody DT, Oyarzun E, Mazor M, Wu YK, Hobbings JC, Durum SK: Infection and labor. III. Interleukin-1: A signal for the onset of labor. Am J Obstet Gynecol. 1989, 160: 1117-1123.View ArticlePubMedGoogle Scholar
- Romero R, Manogue KR, Mitchell MD, Wu YK, Oyarzun E, Hobbins JC, Cerami A: Infection and preterm labor. IV. Cachectin-tumor necrosis factor in the amniotic fluid of women with intraamniotic infection and preterm labor. Am J Obstet Gynecol. 1989, 161: 336-341.View ArticlePubMedGoogle Scholar
- Mitchel MD, Dudley DJ, Edwin SS, Schiller SL: Interleukin-6 stimulates prostaglandin production by human amnion and decidual cells. Eur J Pharmacol. 1991, 192: 189-191. 10.1016/0014-2999(91)90090-D.View ArticleGoogle Scholar
- Mitchell MD, Simpson KL, Keelan JA: Paradoxical proinflammatory actions of interleukin-10 in human amnion: potential role in term and preterm labor. J Clin Endocrinol Metab. 2004, 89: 4149-4152. 10.1210/jc.2004-0373.View ArticlePubMedGoogle Scholar
- Okazaki T, Casey ML, Okita J, MacDonald PC, Johnson JM: Initiation of parturition XII. Biosynthesis and metabolism of prostaglandin in human fetal membranes and uterine decidua. Am J Obstet Gynecol. 1981, 139: 373-381.View ArticlePubMedGoogle Scholar
- Romero R, Mazor M, Sepulveda W, Avila C, Copeland D, Williams J: Tumor necrosis factor in preterm and term labor. Am J Obstet Gynecol. 1992, 166: 1576-1587.View ArticlePubMedGoogle Scholar
- Hunt JS, Chen HL, Miller L: Tumor necrosis factors: pivotal components of pregnancy?. Biol Reprod. 1996, 54: 554-562. 10.1095/biolreprod54.3.554.View ArticlePubMedGoogle Scholar
- Zahl PA, Bjerknes C: Induction of decidua-placental hemorrhage in mice by the endotoxins in certain gram-negative bacteria. Proc Soc Exp Biol Med. 1943, 54: 320-332.View ArticleGoogle Scholar
- Silver RM, Lohner WS, Daynes RA, Mitchell MD, Branch DW: Lipopolysaccharide – induced fetal death: the role of tumor-necrosis factor alpha. Biol reprod. 1994, 50: 1108-1112. 10.1095/biolreprod50.5.1108.View ArticlePubMedGoogle Scholar
- Kent ASH, Sullivan MHF, Elder MG: Transfer of cytokines through human fetal membranes. J Reprod Fertil. 1994, 110: 81-84.View ArticleGoogle Scholar
- Steinborn A, Kühnert M, Halberstadt E: Immunmodulating cytokines induce term and preterm parturition. J Perinat Med. 1996, 24: 381-390.View ArticlePubMedGoogle Scholar
- Keelan JA, Marvin KW, Sato TA, Coleman M, McCowan LME, Mitchell MD: Cytokine abundance in placental tissues: Evidence of inflammatory activation in gestation membranes with term and preterm parturition. Am J Obstet Gynecol. 1999, 181: 1530-1536. 10.1016/S0002-9378(99)70400-X.View ArticlePubMedGoogle Scholar
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