Figure 4

Androgen receptors at the ventral prostate. The execution of consecutive ejaculations had a strong impact on the level of androgen receptors at the ventral prostate. The graph shows that the protein for androgen receptors was detected in males that were not copulating (white bar). However, the concentration increased to its highest value after ejaculations one to three, and then returned to baseline levels after four ejaculations. Western blots at the top show bands for β-actin (β-Ac) and the androgen receptor (AR); mw = molecular weight; 1–4 = number of ejaculations. ** = p < 0.01.