Neural stem cells and the regulation of adult neurogenesis
© Lennington et al; licensee BioMed Central Ltd. 2003
Received: 25 July 2003
Accepted: 13 November 2003
Published: 13 November 2003
Presumably, the 'hard-wired' neuronal circuitry of the adult brain dissuades addition of new neurons, which could potentially disrupt existing circuits. This is borne out by the fact that, in general, new neurons are not produced in the mature brain. However, recent studies have established that the adult brain does maintain discrete regions of neurogenesis from which new neurons migrate and become incorporated into the functional circuitry of the brain. These neurogenic zones appear to be vestiges of the original developmental program that initiates brain formation. The largest of these germinal regions in the adult brain is the subventricular zone (SVZ), which lines the lateral walls of the lateral ventricles. Neural stem cells produce neuroblasts that migrate from the SVZ along a discrete pathway, the rostral migratory stream, into the olfactory bulb where they form mature neurons involved in the sense of smell. The subgranular layer (SGL) of the hippocampal dentate gyrus is another neurogenic region; new SGL neurons migrate only a short distance and differentiate into hippocampal granule cells. Here, we discuss the surprising finding of neural stem cells in the adult brain and the molecular mechanisms that regulate adult neurogenesis.
Discovery of Adult Neurogenesis
The concept of neurogenesis in the adult brain has only recently gained wide acceptance, though support had slowly been building over the past century. As early as 1912, and subsequently in 1932, 1938 and 1944, cytological investigations examining mitotic divisions in the postnatal and adult rodent brain revealed the SVZ as mitotically active (for review, [1, 2]). The advent of autoradiography then made possible the identification of DNA synthesis by incorporation of tritiated thymidine into nascent strands of DNA allowing cell proliferation to be monitored in the mammalian SVZ [3–5]. However, the fate of these proliferating cells was not clear; most cells produced in the SVZ during embryonic development were thought to differentiate into neurons, while only the production of glial cells was described in the postnatal and adult SVZ. Studies lagged until improved immunohistochemical techniques allowed more definitive characterization and identification of SVZ progeny. Interestingly, it was studies in songbirds that inspired the advance of mammalian neurogenesis. In 1983 Nottebohm and colleagues demonstrated that new neurons are produced in the telencephalon of adult male songbirds . These neurons appear to be required for the production of new elemental components of song that allow embellishment of song each season . In addition, it was found that acquisition of new neurons is hormonally regulated and therefore seasonally controlled to correspond to the mating season . Full acceptance of postembryonic mammalian neurogenesis did not take hold until just over a decade ago when Luskins  described neurogenesis in the anterior portion of the SVZ in postnatal mice, Lois and Alvarez-Buylla  demonstrated neurogenesis in the adult mouse SVZ and several groups built upon previously shown neurogenesis in the SGL of the hippocampus [10–13]. The possibility for other adult neurogenic regions exists and the search for sites of adult neurogenesis continues.
The SVZ Niche: The Largest Site of Adult Neurogenesis
The presence of neural stem cells in the SVZ was suggested based on the finding that a subpopulation of SVZ cells can be dissociated and grown as free-floating spheres, neurospheres, in culture in the presence of mitogen (EGF or FGF2) . Some of these primary spheres are capable of generating secondary spheres upon dissociation and can be renewed through many passages. This self-renewing population is multipotent, capable of generating neurons, oligodendrocytes and astrocytes, upon removal of mitogen. Thus, these cells qualify in fulfilling the criteria of stem cells: self-renewable and multipotential. However, the precise identification of neural stem cells in vivo is currently mired by the lack of sensitive and specific immunological markers to identify cell types of the SVZ. Recent studies in which the SVZ was temporarily depleted of proliferating cells using the anti-mitotic agent cytosine-β-D-arabinofuranoside (Ara-C) revealed that cells positive for glial fibrillary acidic protein (GFAP) and displaying astrocytic characteristics repopulated the SVZ upon withdrawal of Ara-C . In addition, these GFAP-positive cells are capable of generating neurospheres . Most recent studies now support the claim that neural stem cells have some characteristics of astrocytes [20–22]. However, it is unlikely that all astrocytes are neural stem cells, so it becomes important to distinguish the subpopulation of SVZ astrocytes that are neural stem cells. Cell-specific markers together with investigations into the regulatory mechanisms that contribute to the maintenance of stem cell renewal and neurogenesis will reveal how this adult germinal zone persists into adulthood.
Hormonal Influences on Adult Neurogenesis
Studies conducted over the past several years have identified steroid hormones (e.g., adrenal steroids, testosterone and estrogen) and peptide hormones (such as prolactin) as potential regulators of adult neurogenesis. The cyclical fluctuations of sex hormone levels raise the possibility of corresponding cyclical waves of neurogenesis.
Estrogen and Testosterone
While estradiol's effect is in the hippocampal SGL and does not affect cell proliferation in the SVZ, prolactin stimulates the production of neuronal progenitors in the SVZ  (Fig. 2a). Prolactin is a hormone that increases during the first half of pregnancy and also at postpartum, signaling lactation. A recent study showed that neurogenesis rates jump during pregnancy by 65%, peaking on the seventh day of the mouse's 21-day gestation period and again after delivery . In addition to observing cell proliferation, this study tracked integration of the new neurons in the OB. The premise behind this study is that olfactory discrimination is critical for recognition and rearing of offspring. A doubling of olfactory interneurons may thereby enhance olfactory function following pregnancy, providing the mother with enhanced olfactory capability . The increase in olfactory neurons associated with pregnancy and the implicated heightened sense of smell does not come as a surprise to anyone who has experienced pregnancy and the associated acute sense of smell that can often trigger nausea.
Corticosteroids/Adrenal stress steroids
Adrenal steroids, on the other hand, have been shown to inhibit adult neurogenesis by suppressing cell proliferation in the hippocampal SGL (Fig. 2a). Aged rats and monkeys exhibit diminished cell proliferation in the SGL as well as elevated levels of circulating glucocorticoids [13, 35, 36]. Removal of adrenal steroids by adrenalectomy increases cell proliferation in the SGL in both aged and young adult rats . Moreover, the number of new SGL cells in adrenalectomized aged rats was threefold higher than the number in young control rats, indicating that adrenalectomized aged rats have rates of proliferation that surpass those normally found in young adults . The absence of adrenal steroid receptors on granule cell precursors in the SGL suggests that the effects of adrenal steroids on cell proliferation occur indirectly through other factors .
Regulation and Maintenance of the SVZ
The SVZ is a specialized niche where functions such as cell fate, cell adhesion, migration, polarity and proliferation are regulated in part by secreted and membrane-bound ligands signaling through their cognate receptors (for review see ). These initiating extracellular signals then set off a cascade of intracellular reactions that ultimately control cellular gene expression. Below we discuss recent findings and associated controversies concerning six ligand/receptor families and their roles in SVZ function.
EGF and FGF2
SVZ neural stem cells continue to generate new neurons in the adult brain. When dissociated from the adult SVZ, neural stem cells require either epidermal growth factor (EGF) or basic fibroblast growth factor (FGF2) for self-renewal and long-term survival in culture [18, 39]. Analysis of EGF and FGF2 responsiveness in the developing telencephalon indicates that early growth factor choice is temporally regulated [40, 41]. FGF2 response is present as early as E8.5, a time when EGF receptors (EGFRs) are not expressed on neural stem cells. By E14.5 neural stem cells expressing EGFR emerge . In the adult, the vast majority of SVZ cells expressing EGFR also express FGFR1 supporting the finding that most EGF-responsive cells can also be stimulated by FGF2 . However, EGF and FGF2 appear to differ in their mechanisms of support, with EGF promoting faster expansion of the stem cell-like pool (symmetric division) compared to FGF2 . This may be the result of differential control of cell cycle length by each growth factor, with SVZ stem-like cells cycling faster in the presence of EGF . Alternatively, since the SVZ has two subsets of mitotically active cells, the neural stem cells (a relatively quiescent population with a cell cycle length up to 28 days) [43, 44], and the transitory amplifying progenitor (TAP) cells (cell cycle length approximately 12 h), these two growth factors may preferentially target one cell type (Fig. 2b). The latter appears to be supported by the work of Kuhn et al.  and Doetsch et al. . Kuhn et al. found that intracerebroventricular infusion of FGF2 into the lateral ventricle resulted in increased numbers of new neurons in the OB, while EGF infusion reduced the number of neurons reaching the OB, but substantially increased generation of astrocytes in the OB and the neighboring striatum. Extension of this study by Doetsch et al.  suggests that TAP cells are EGF receptive and these cells become invasive and glia-like, diverting neurogenesis to gliogenesis. In contrast, Craig et al.  found increased SVZ neurogenesis and associated migration of neuroblasts into the OB following a 6 day infusion of EGF, similar to FGF2 infusion. It is hard to reconcile these disparate results, however, improving our ability to uniquely identify neural stem cells (likely a subset of SVZ astrocytes)  will allow discrimination between neural stem cells and SVZ progenitor cells and aid in our understanding of the molecular dynamics regulating this germinal zone.
CNTF and LIF
Other signaling pathways have been implicated in the decision between self-renewal through symmetric division or promotion of neural stem cell differentiation. The cytokine ciliary neurotrophic factor (CNTF) signaling through its heterotrimeric receptor complex of CNTF receptor α, LIF receptor β and gp130 subunits supports embryonic stem cell self-renewal and pluripotency  and has recently been reported to support neural stem cell self-renewal [47, 48] (Fig. 2b). This action appears to be mediated via Notch signaling .
Activation of the Notch1 receptor inhibits neurogenesis (Fig. 2b). Controversy then arises as to whether Notch signaling maintains stem cell pluripotency or actively instructs glial cell fate (for review see ). Neural stem cells are depleted in Notch-/- mice and in mice lacking key regulators of Notch signaling activity . However, transient Notch activation induced by exogenous Notch ligand caused rapid and irreversible loss of neurogenic capacity accompanied by accelerated glial differentiation . Similarly, introduction of activated Notch into the mouse embryonic forebrain by retroviral vector and tracked by ultrasound imaging revealed that Notch–infected cells became radial glia . Recent findings indicate that proliferative radial glia, while originally thought to be part of the glial lineage, can also function as neuronal precursors [52, 53] (for review see ). Interestingly, many of the Notch-infected cells eventually became periventricular astrocytes, the same cells shown to be the neural stem cells in the adult SVZ , evoking the interesting possibility that radial glia and SVZ astrocytes are of the same lineage . This possibility helps to resolve the apparent contradictory findings that Notch signaling is instructive for gliogenesis and for maintaining neural stem cells in an undifferentiated state (Fig. 2b).
What then are the instructive signals for neurogenesis in the SVZ? The neurogenic environment of the SVZ has been attributed in part to the local antagonistic interplay between noggin and bone morphogenetic proteins (BMPs) . BMPs are negative regulators of neural determination; noggin reverses this effect by binding BMPs, preventing their signal activation [55, 56] (Fig. 2b). BMPs are expressed by the SVZ cells, while noggin is expressed by ependymal cells . This arrangement of interacting signals from the ependymal cells and the adjacent SVZ may provide the necessary regulation of neurogenesis and gliogenesis in the adult brain.
Much still needs to be learned about how extracellular signaling pathways coordinate the intricate balance of neurogenesis, gliogenesis and stem cell renewal in the adult SVZ. From the data accumulated so far, it does appear that neurogenic strategies of the adult SVZ recapitulate some themes and mechanisms used in the developing embryonic nervous system. Thus, information gathered from one developmental system will offer clues as to how the other system may function.
We would like to thank and acknowledge Virge Kask for artwork in Figure 1. This work is supported by NIH grant R21 NS45894-01 (to JCC)
- Alvarez-Buylla A, Garcia-Verdugo JM, Tramontin AD: A unified hypothesis on the lineage of neural stem cells. Nature Reviews. 2001, 2: 287-293. 10.1038/35067582.View ArticlePubMedGoogle Scholar
- Rakic P: Adult neurogenesis in mammals: an identity crisis. J Neurosci. 2002, 22 (3): 614-618.PubMedGoogle Scholar
- Smart I: The subependymal layer of the mouse brain and its cell production as shown by radioautography after thymidine-H3 injection. J Comp Neurol. 1961, 116: 325-347.View ArticleGoogle Scholar
- Altman J: Autoradiographic investigation of cell proliferation in the brains of rats and cats. Anat Rec. 1963, 145: 573-591.View ArticlePubMedGoogle Scholar
- Altman J: Are neurons formed in the brains of adult mammals?. Science. 1962, 135: 1127-1128.View ArticlePubMedGoogle Scholar
- Goldman SA, Nottebohm F: Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain. Proc Natl Acad Sci U S A. 1983, 80 (8): 2390-2394.PubMed CentralView ArticlePubMedGoogle Scholar
- Nottebohm F: A brain for all seasons: cyclical anatomical changes in song control nuclei of the canary brain. Science. 1981, 214 (4527): 1368-1370.View ArticlePubMedGoogle Scholar
- Luskins MB: Restricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone. Neuron. 1993, 11: 173-189.View ArticleGoogle Scholar
- Lois C, Alvarez-Buylla A: Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia. Proc Natl Acad Sci U S A. 1993, 90 (5): 2074-2077.PubMed CentralView ArticlePubMedGoogle Scholar
- Altman J, Das GD: Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats. J Comp Neurol. 1965, 124 (3): 319-335.View ArticlePubMedGoogle Scholar
- Kaplan MS, Bell DH: Mitotic neuroblasts in the 9-day-old and 11-month-old rodent hippocampus. J Neurosci. 1984, 4 (6): 1429-1441.PubMedGoogle Scholar
- Cameron HA, Woolley CS, McEwen BS, Gould E: Differentiation of newly born neurons and glia in the dentate gyrus of the adult rat. Neuroscience. 1993, 56 (2): 337-344. 10.1016/0306-4522(93)90335-D.View ArticlePubMedGoogle Scholar
- Kuhn HG, Dickinson-Anson H, Gage FH: Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. J Neurosci. 1996, 16 (6): 2027-2033.PubMedGoogle Scholar
- Doetsch F, Garcia-Verdugo JM, Alvarez-Buylla A: Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain. J Neurosci. 1997, 17: 5046-5061.PubMedGoogle Scholar
- Alvarez-Buylla A, Garcia-Verdugo JM: Neurogenesis in adult subventricular zone. J Neurosci. 2002, 22 (3): 629-634.PubMedGoogle Scholar
- Conover JC, Allen RL: The subventricular zone: new molecular and cellular developments. Cell Mol Life Sci. 2002, 59 (12): 2128-2135. 10.1007/s000180200012.View ArticlePubMedGoogle Scholar
- Doetsch F, Petreanu L, Caille I, Garcia-Verdugo JM, Alvarez-Buylla A: EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells. Neuron. 2002, 36 (6): 1021-1034.View ArticlePubMedGoogle Scholar
- Reynolds BA, Weiss S: Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 1992, 255 (5052): 1707-1710.View ArticlePubMedGoogle Scholar
- Doetsch R, Caille I, Lim DA, Garcia-Verdugo JM, Alvarez-Buylla A: Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell. 1999, 97: 703-716.View ArticlePubMedGoogle Scholar
- Imura T, Kornblum HI, Sofroniew MV: The predominant neural stem cell isolated from postnatal and adult forebrain but not early embryonic forebrain expresses GFAP. J Neurosci. 2003, 23 (7): 2824-2832.PubMedGoogle Scholar
- Laywell ED, Rakic P, Kukekov VG, Holland EC, Steindler DA: Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain. Proc Natl Acad Sci U S A. 2000, 97 (25): 13883-13888. 10.1073/pnas.250471697.PubMed CentralView ArticlePubMedGoogle Scholar
- Chiasson BJ, Tropepe V, Morshead CM, van der Kooy D: Adult mammalian forebrain ependymal and subependymal cells demonstrate proliferative potential, but only subependymal cells have neural stem cell characteristics. J Neurosci. 1999, 19 (11): 4462-4471.PubMedGoogle Scholar
- Nordeen EJ, Nordeen KW: Estrogen stimulates the incorporation of new neurons into avian song nuclei during adolescence. Brain Res Dev Brain Res. 1989, 49 (1): 27-32. 10.1016/0165-3806(89)90056-4.View ArticlePubMedGoogle Scholar
- Rasika S, Nottebohm F, Alvarez-Buylla A: Testosterone increases the recruitment and/or survival of new high vocal center neurons in adult female canaries. Proc Natl Acad Sci U S A. 1994, 91 (17): 7854-7858.PubMed CentralView ArticlePubMedGoogle Scholar
- Hidalgo A, Barami K, Iversen K, Goldman SA: Estrogens and non-estrogenic ovarian influences combine to promote the recruitment and decrease the turnover of new neurons in the adult female canary brain. J Neurobiol. 1995, 27 (4): 470-487.View ArticlePubMedGoogle Scholar
- Johnson F, Bottjer SW: Differential estrogen accumulation among populations of projection neurons in the higher vocal center of male canaries. J Neurobiol. 1995, 26 (1): 87-108.View ArticlePubMedGoogle Scholar
- Maren S, De Oca B, Fanselow MS: Sex differences in hippocampal long-term potentiation (LTP) and Pavlovian fear conditioning in rats: positive correlation between LTP and contextual learning. Brain Res. 1994, 661 (1–2): 25-34. 10.1016/0006-8993(94)91176-2.View ArticlePubMedGoogle Scholar
- Roof RL, Havens MD: Testosterone improves maze performance and induces development of a male hippocampus in females. Brain Res. 1992, 572 (1–2): 310-313. 10.1016/0006-8993(92)90491-Q.View ArticlePubMedGoogle Scholar
- Roof RL, Zhang Q, Glasier MM, Stein DG: Gender-specific impairment on Morris water maze task after entorhinal cortex lesion. Behav Brain Res. 1993, 57 (1): 47-51. 10.1016/0166-4328(93)90060-4.View ArticlePubMedGoogle Scholar
- Galea LA, Kavaliers M, Ossenkopp KP: Sexually dimorphic spatial learning in meadow voles Microtus pennsylvanicus and deer mice Peromyscus maniculatus. J Exp Biol. 1996, 199 (Pt 1): 195-200.PubMedGoogle Scholar
- Kavaliers M, Ossenkopp KP, Prato FS, Innes DG, Galea LA, Kinsella DM, Perrot-Sinal TS: Spatial learning in deer mice: sex differences and the effects of endogenous opioids and 60 Hz magnetic fields. J Comp Physiol [A]. 1996, 179 (5): 715-724.View ArticleGoogle Scholar
- Tanapat P, Hastings NB, Reeves AJ, Gould E: Estrogen stimulates a transient increase in the number of new neurons in the dentate gyrus of the adult female rat. J Neurosci. 1999, 19 (14): 5792-5801.PubMedGoogle Scholar
- Banasr M, Hery M, Brezun JM, Daszuta A: Serotonin mediates oestrogen stimulation of cell proliferation in the adult dentate gyrus. Eur J Neurosci. 2001, 14 (9): 1417-1424. 10.1046/j.0953-816x.2001.01763.x.View ArticlePubMedGoogle Scholar
- Shingo T, Gregg C, Enwere E, Fujikawa H, Hassam R, Geary C, Cross JC, Weiss S: Pregnancy-stimulated neurogenesis in the adult female forebrain mediated by prolactin. Science. 2003, 299 (5603): 117-120. 10.1126/science.1076647.View ArticlePubMedGoogle Scholar
- Gould E, Reeves AJ, Fallah M, Tanapat P, Gross CG, Fuchs E: Hippocampal neurogenesis in adult Old World primates. Proc Natl Acad Sci U S A. 1999, 96 (9): 5263-5267. 10.1073/pnas.96.9.5263.PubMed CentralView ArticlePubMedGoogle Scholar
- Sapolsky RM: Do glucocorticoid concentrations rise with age in the rat?. Neurobiol Aging. 1992, 13 (1): 171-174. 10.1016/0197-4580(92)90025-S.View ArticlePubMedGoogle Scholar
- Cameron HA, McKay RD: Restoring production of hippocampal neurons in old age. Nat Neurosci. 1999, 2 (10): 894-897. 10.1038/13197.View ArticlePubMedGoogle Scholar
- Cameron HA, Woolley CS, Gould E: Adrenal steroid receptor immunoreactivity in cells born in the adult rat dentate gyrus. Brain Res. 1993, 611 (2): 342-346. 10.1016/0006-8993(93)90524-Q.View ArticlePubMedGoogle Scholar
- Kuhn HG, Winkler J, Dempermann G, Thal LJ, Gage FH: Epidermal growth factor and fibroblast growth factor-2 have different effects on neural progenitors in the adult rat brain. J Neurosci. 1997, 17 (15): 5820-5829.PubMedGoogle Scholar
- Tropepe V, Sibilia M, Ciruna BG, Rossant J, Wagner EF, van der Kooy D: Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol. 1999, 208 (1): 166-188. 10.1006/dbio.1998.9192.View ArticlePubMedGoogle Scholar
- Maric D, Maric I, Chang YH, Barker JL: Prospective cell sorting of embryonic rat neural stem cells and neuronal and glial progenitors reveals selective effects of basic fibroblast growth factor and epidermal growth factor on self-renewal and differentiation. J Neurosci. 2003, 23 (1): 240-251.PubMedGoogle Scholar
- Gritti A, Frolichsthal-Schoeller P, Galli R, Parati EA, Cova L, Pagano SF, Bjornson CR, Vescovi AL: Epidermal and fibroblast growth factors behave as mitogenic regulators for a single multipotent stem cell-like population from the subventricular region of the adult mouse forebrain. J Neurosci. 1999, 19 (9): 3287-3297.PubMedGoogle Scholar
- Morshead CM, van der Kooy D: Postmitotic death is the fate of constitutively proliferating cells in the subependymal layer of the adult mouse brain. J Neurosci. 1992, 12 (1): 249-256.PubMedGoogle Scholar
- Morshead CM, Reynolds BA, Craig CG, McBurney MW, Staines WA, Morassutti D, Weiss S, van der Kooy D: Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells. Neuron. 1994, 13 (5): 1071-1082.View ArticlePubMedGoogle Scholar
- Craig CG, Tropepe V, Morshead CM, Reynolds BA, Weiss S, van der Kooy D: In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain. J Neurosci. 1996, 16 (8): 2649-2658.PubMedGoogle Scholar
- Conover JC, Ip NY, Poueymirou WT, Bates B, Goldfarb MP, DeChiara TM, Yancopoulos GD: Ciliary neurotrophic factor maintains the pluripotentially of embyronic stem cells. Development. 1993, 119: 559-565.PubMedGoogle Scholar
- Shimazaki T, Shingo T, Weiss S: The ciliary neurotrophic factor/leukemia inhibitory factor/gp130 receptor complex operates in the maintenance of mammalian forebrain neural stem cells. J Neurosci. 2001, 21 (19): 7642-7653.PubMedGoogle Scholar
- Chojnacki AT, Shimazaki C, Gregg G, Weinmaster G, Weiss S: Glycoprotein 130 signaling regulates Notch1 expresssions and activation in the self-renewal of mammalian forebrain neural stem cells. Journal of Neuroscience. 2003, 23 (5): 1730-1741.PubMedGoogle Scholar
- Hitoshi S: Notch pathway molecules are essential for the maintenance, but not the generation, of mammalian neural stem cells. Genes Dev. 2002, 16 (7): 846-858. 10.1101/gad.975202.PubMed CentralView ArticlePubMedGoogle Scholar
- Morrison SJ, Perez SE, Qiao Z, Verdi JM, Hicks C, Weinmaster G, Anderson DJ: Transient Notch activation initiates an irreversible switch from neurogenesis to gliogenesis by neural crest stem cells. Cell. 2000, 101 (5): 499-510.View ArticlePubMedGoogle Scholar
- Gaiano N, Nye JS, Fishell G: Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Neuron. 2000, 26 (2): 395-404.View ArticlePubMedGoogle Scholar
- Malatesta P, Hartfuss E, Gotz M: Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineage. Development. 2000, 127 (24): 5253-5263.PubMedGoogle Scholar
- Noctor SC, Flint AC, Weissman TA, Dammerman RS, Kriegstein AR: Neurons derived from radial glial cells establish radial units in neocortex. Nature. 2001, 409 (6821): 714-720. 10.1038/35055553.View ArticlePubMedGoogle Scholar
- Lim DA, Tramontin AD, Trevejo JM, Herrera DG, Garcia-Verdugo JM, Alvarez-Buylla A: Noggin antagonizes BMP signaling to create a niche for adult neurogenesis. Neuron. 2000, 28 (3): 713-726.View ArticlePubMedGoogle Scholar
- Gross RE, Mehler MF, Mabie PC, Zang Z, Santschi L, Kessler JA: Bone morphogenetic proteins promote astroglial lineage commitment by mammalian subventricular zone progenitor cells. Neuron. 1996, 17 (4): 595-606.View ArticlePubMedGoogle Scholar
- Shou J, Rim PC, Calof AL: BMPs inhibit neurogenesis by a mechanism involving degradation of a transcription factor. Nat Neurosci. 1999, 2 (4): 339-345. 10.1038/7251.View ArticlePubMedGoogle Scholar
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