Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea
© Ndiaye et al; licensee BioMed Central Ltd. 2010
Received: 7 April 2010
Accepted: 3 August 2010
Published: 3 August 2010
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© Ndiaye et al; licensee BioMed Central Ltd. 2010
Received: 7 April 2010
Accepted: 3 August 2010
Published: 3 August 2010
The endoplasmic reticulum (ER) is a major site of protein synthesis and facilitates the folding and assembly of newly synthesized proteins. Misfolded proteins are retrotranslocated across the ER membrane and destroyed at the proteasome. DERL1 is an important protein involved in the retrotranslocation and degradation of a subset of misfolded proteins from the ER. We characterized a 2617 bp cDNA from bovine granulosa cells that corresponded to bovine DERL1. Two transcripts of 3 and 2.6 kb were detected by Northern blot analysis, and showed variations in expression among tissues. During follicular development, DERL1 expression was greater in day 5 dominant follicles compared to small follicles, ovulatory follicles, or corpus luteum (CL). Within the CL, DERL1 mRNA expression was intermediate in midcycle, and lowest in late cycle as compared to early in the estrous cycle. Western blot analyses demonstrated the presence of DERL1 in the bovine CL at days 5, 11, and 18 of the estrous cycle. Co-immunoprecipitation using luteal tissues showed that DERL1 interacts with class I MHC but not with VIMP or p97 ATPase. The interaction between DERL1 and MHC I suggests that, in the CL, DERL1 may regulate the integrity of MHC I molecules that are transported to the ER membrane. Furthermore, the greater expression of DERL1 mRNA is associated with the active follicular development and early luteal stages, suggesting a role of DERL1 in tissue remodeling events and maintenance of function in reproductive tissues.
The endoplasmic reticulum (ER) is the first compartment of the secretory pathway and is a processing station for secreted and transmembrane proteins. The primary function of the ER is to assist newly synthesized proteins to refold into native conformation. To achieve correct folding and maturation, secreted proteins must translocate into the ER to undergo several post-translational modifications, including glycosylation and disulfide bonding . Proteins that fail to fold properly in the ER are recognized by the quality control machinery and transferred from the ER into the cytosol to the ubiquitin-proteasome system in a process called retrotranslocation or dislocation . The ER-associated degradation (ERAD) machinery serves as one part of the adaptive cellular program to destroy the potentially toxic accumulation of misfolded proteins. A dysfunction in the ERAD pathway causes human diseases  while many viral proteins can hijack this pathway to evade detection by the immune system .
This latter phenomenon is used by the human cytomegalovirus (HCMV) to eliminate class I major histocompatibility complex (MHC I) molecules to distract cytotoxic T cells from destroying infected cells . The HCMV encodes the glycoproteins US2 and US11 that induce retrotanslocation of newly synthesized class I heavy chains . The HCMV US2 and US11 proteins utilize the normal ER quality control process to eliminate class I heavy chains in a similar manner as misfolded or damaged ER proteins. The ERAD of class I MHC heavy chain by HCMV-encoded US11 involves DERL1, a mammalian ER membrane protein, homolog of Der1p in Saccharomyces cerevisiae that is essential for the relocation of misfolded proteins from the ER lumen into the cytosol [7, 8]. This action of DERL1 is performed in concert with the ATPase p97/Valosin-containing protein (VCP) that forms a complex with DERL1 [7, 8] after being recruited by the VCP-interacting membrane protein (VIMP), which can interact with DERL1 .
The expressions of class I and class II MHC molecules have been demonstrated in the corpus luteum (CL) where they may function to regulate T lymphocyte activation, thus controlling the production of cytokines [9–13]. The CL is a very heterogeneous population of cells that becomes rapidly organized into a functional unit. These diverse cells communicate both directly and through paracrine mediators to facilitate the steroidogenic function and the transient nature of the CL. The CL also contains nonsteroidogenic cells, including endothelial cells, fibroblasts, and immune cells that have a role in luteal function by communicating with steroidogenic cells through the paracrine signaling molecules they produce and through direct cell contacts [14, 15]. The MHC molecule-dependent interaction between luteal cells and T cells is one form of direct cell-cell signaling that may serve to activate resident immune cells . An association between MHC proteins and the ERAD proteins in reproductive tissues has not been previously investigated. Thus, studying the link between class I MHC and DERL1 in the CL may contribute to understanding the control of luteal function.
There has been significant interest in the role of DERL1 in the ER quality control machinery. However, nothing is known about the expression of DERL1, its regulation and physiological relevance in reproductive tissues, such as the ovary. In this study, the expression of DERL1 in bovine granulosa and luteal cells, and its regulation at different stages of follicular growth and corpora lutea development were analyzed. Using normal luteal tissue extracts, putative interactions of DERL1 with MHC I, p97 ATPase, and VIMP were also investigated.
The regulation of DERL1 mRNA expression during follicular development and ovulation was studied using in vivo models as previously characterized . Briefly, estrous cycles of normally cyclic crossbred heifers were synchronized with one injection of prostaglandin F2 alpha (PGF2α; 25 mg, im; Lutalyse, Upjohn, Kalamazoo, MI) given in the presence of a CL. Ovarian follicular development was monitored by daily transrectal ultrasonography. Following estrous synchronization, heifers were randomly assigned to the dominant follicle group (DF, n = 4), or the ovulatory, hCG-induced follicle group (OF, n = 4). Immediately following ovariectomy, granulosa cells (GC) were collected separately from individual DF or OF as described previously , and stored at -70°C. Additionally, GC were collected from 2-4 mm small follicles (SF) obtained from slaughterhouse ovaries, and a total of three pools of 10-20 SF was prepared. Corpora lutea at day 5 of the estrous cycle were obtained by ovariectomy and were dissected from the ovarian stroma, frozen in liquid nitrogen, and stored at -70°C until RNA was extracted. The Animal Ethics Committee of the Faculty of Veterinary Medicine of the University of Montreal approved all animal procedures.
Corpora lutea were collected transvaginally from cyclic cows at early (day 5) mid (day 11) and late (day 18) luteal phase of the estrous cycle (day of estrus = day 0) and at 1, 4, 8, and 12 hours after a 25-mg injection of PGF2α (Lutalyse; Upjohn, Kalamazoo, MI) in the midluteal phase to induce luteal regression. Total RNA was extracted from luteal tissues to quantify DERL1 mRNA concentrations by real time-quantitative PCR. Corpora lutea collection was performed at the Ohio State University and handling of animals and surgical procedures were conducted according to protocols approved by the Institutional Laboratory Animal Care and Use Committee of the Ohio State University.
The DERL1 cDNA was cloned from a bovine cDNA library prepared with polyA+ mRNA isolated from granulosa cells of dominant follicles at day 5 of the estrous cycle. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as described . Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON). The cDNA inserts were analyzed by sequencing, and sequences were compared by BLAST in GenBank database. Alignment of deduced amino acid sequences of bovine DERL1 protein with putative homologs from other organisms was performed using the ClustalW2 analysis software (EMBL-EBI, Hinxton, Cambridge, UK). DERL1 cDNA was entirely characterized by sequencing on an ABI Prism 310 (Applied BioSystem).
Various bovine tissues were obtained from a local slaughterhouse and RNA was extracted in lysis buffer (for 500 ml buffer: 4 M guanidium isothiocyanate, 2.5 ml Na-N-laurylsarcosine, 25 mM Na-citrate, pH 7), and sedimented, following centrifugation on a cesium chloride cushion as described . The concentration of total RNA was quantified by measurement of optical density at 260 nm, and RNA integrity was evaluated by visualizing the 28 S and 18 S ribosomal RNA bands following electrophoretic separation on 0.66 M formaldehyde denaturing 1% agarose gel with ethidium bromide. Total RNA (20 μg/tissue) were size-fractionated on a 0.66 M formaldehyde, 1% agarose gel, transfered by capillarity to a nylon membrane (Hybond-N; Amersham Pharmacia Biotech), and UV-treated (150 mJ) as described . The bovine DERL1 cDNA was used to generate a radioactive probe incorporating [α32P]-dCTP (NEN Life Sciences, Boston, MA) that was used to hybridize Northern blots as described .
Analysis for the presence of putative alternative splicing variants for bovine DERL1 mRNA was performed by RT-PCR using total RNA from bovine CL. The first step consisted of a reverse transcription reaction performed with 1 μg of total RNA using the M-MLV Reverse Transcriptase (Promega) following the manufacturer's protocol. The second step consisted of a PCR amplification with specific primers (foward: 5'-ATGTCGGACATCGGGGACTGG-3'; reverse: 5'-CTGGTCCCCGAGCCGAAAGC-3') located respectively at the start codon and stop codon of the DERL1 open reading frame (ORF) amplifying the entire ORF of 754 bp in a 35 cycle PCR reaction. The PCR products were analyzed for size and specificity using the Agilent DNA 1000 kit on the Agilent 2100 BioAnalyzer (Agilent Technologies).
Expression of DERL1 mRNA during follicular development was analyzed by semi-quantitative RT-PCR. Total RNA was extracted from bovine granulosa cells (GC) collected from 2-4 mm small follicles (SF), dominant follicles at day 5 of the estrous cycle (DF), ovulatory follicles 24 hours after an injection of hCG (OF), and from day 5 corpora lutea (CL). DERL1 PCR primers were as follows: (forward: 5'-TATCGCTTCCAGATTTGGAGG-3'; reverse: 5'-GAATCCAAGGATAACCCAAGG-3'). The number of cycles was limited and optimized for analysis of DERL1 mRNA expression. PCR reaction products were separated on a 2% TAE-agarose gel with ethidium bromide, visualized by UV light, digitized and analyzed by densitometry using ImageQuant software (Amersham Pharmacia Biotechniques). GAPDH was used as a control gene, and specific signals of DERL1 were normalized with corresponding GAPDH signals.
Total RNA was extracted from luteal tissues using Trizol reagent as described , quantified using the Nanodrop spectrophotometer (Thermo Scientific), and treated with RNase-free DNase I (Roche Molecular Biochemicals) to eliminate genomic DNA contamination. qPCR was used to detect and quantify DERL1 mRNA. Oligonucleotide primers specific for DERL1 transcript, described in the semi-quantitative RT-PCR section, were used to amplify DERL1 cDNA. Ribosomal protein L19 (RPL19) cDNA fragment was amplified as a constitutively expressed gene with the following primers (forward: 5'-ATCGATCGCCACATGTATCA-3'; reverse: 5'-GCGTGCTTCCTTGGTCTTAG-3'). Total RNA (1 μg) was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories) according to the protocol of the manufacturer. Following the reverse transcription reaction, quantitative PCR was performed on the MJ Research Opticon 2 (Bio-Rad Laboratories) as described  using the iQSYBR Green Supermix (Bio-Rad Laboratories). Homologous standard curve prepared from purified DERL1 cDNA PCR product was used to calculate the relative steady-state concentrations of DERL1 mRNA in triplicate wells for each sample. The PCR amplification products were electrophoretically separated on 1.5% agarose gels and visualized with ethidium bromide. For initial validation, the specific band corresponding to the size of the expected DERL1 cDNA fragment was cut and purified using the QIAquick Gel Extraction Kit (Qiagen Sciences) for sequence confirmation. A control sample that was not reverse transcribed was used to confirm that the product obtained was not amplified from genomic DNA.
Proteins were extracted from luteal tissues at days 5, 11, and 18 of the estrous cycle using the CelLytic MT Cell Lysis Reagent (Sigma-Aldrich Biotechnology) in the presence of the protease inhibitor cocktail (Sigma-Aldrich Biotechnology) following the manufacturer's protocol. Proteins were quantified according to the method of Bradford  (Bio-Rad Protein Assay, Bio-Rad Laboratories). Protein samples (75 μg) were subjected to electrophoresis on a 12% SDS-polyacrylamide gel, and the separated proteins were blotted onto polyvinylidene difluoride membranes (PVDF; Hybond-P, Amersham Pharmacia Biotech). Western blot analyses were performed exactly as described  using a polyclonal rabbit anti-DERL1 antibody (NB100-447SS; Novus Biologicals), raised against a synthetic peptide corresponding to amino acid residues 150-250 of human DERL1 at a final concentration of 0.5 μg/ml. Membranes were then washed as described  and incubated with the horseradish peroxidase-labeled anti-rabbit secondary antibody (Amersham Biosciences) at a dilution of 1:20 000. The antigen-antibody complex was visualized using the enhanced chemiluminescence system (ECL Western Blotting Analysis System; Amersham Biosciences) following the manufacturer's protocol. Membranes were exposed to Kodak Biomax light films (Kodak), and the films were developed in the SRX-101A Konica film processor (Konica Corporation, Japan). Protein bands were analyzed by densitometry using the Kodak Image Station 4000R software (Eastman Kodak Co.). Beta actin was used as an internal control to verify the integrity of proteins in the samples and specific signals of DERL1 were normalized with corresponding beta actin signals.
Protein samples were obtained from luteal tissues as described above. Immunoprecipitation of DERL1 was performed following the protocol as described . Protein lysates (100 μg) were first incubated with 100 μl of S. aureus Cowan I (Pansorbin, Calbiochem) saturated with normal rabbit serum and 50 μl of Sepharose CL-4B (Amersham Pharmacia Biotech). After an overnight incubation at 4°C with continuous gentle rocking followed by centrifugation (10,600 g, 15 min, 4°C), the supernatant was collected and incubated with 15 μl of specific anti-DERL1 antibodies with rocking for 2 hours at 4°C. The antigen-antibody complex was precipitated by the addition of 80 μl of protein A Sepharose CL-4B (Amersham Pharmacia Biotech) for 1 hour at 4°C with rocking followed by centrifugation (10,600 g, 1 min, 4°C) to pellet the complex. Supernatants were collected at this point and stored at -70°C for further analysis. The pelleted precipitates containing DERL1 and potential bound proteins were washed 3 times in lysis buffer containing 1% Nonidet-P40. Both precipitate and corresponding supernatant samples were heated at 90°C for 5 min in the presence of Laemmli loading buffer and separated on SDS-polyacrylamide gel alongside molecular weight markers (Bio-Rad Laboratories). Proteins were transferred onto PVDF membranes and blotted separately with specific antibodies against class I MHC (H17A; VMRD), VIMP (V6639; Sigma), and p97 (PRO65278; Research Diagnostics Inc.). Additional anti-DERL1 antibodies were obtained from Sigma (D4443) in order to confirm the presence of DERL1 in the precipitates.
For the data from semi-quantitative PCR experiments, gene-specific signals were normalized with corresponding GAPDH signals for each sample. Homogeneity of variance among follicular groups and CL was verified by O'Brien and Brown-Forsythe tests. Corrected values of gene-specific mRNA were compared by one-way ANOVA in GC obtained from bovine follicles collected at different developmental stages or CL. When ANOVA showed significant differences, Tukey-Kramer multiple comparisons was used to determine differences at p < 0.05. The same statistical procedure was used for the western blot data with specific DERL1 signals normalized with corresponding beta actin signals for each sampling day. All other statistical analyses were performed using the covariate analysis within the mixed model of SAS (SAS Inst. Inc., Cary, NC) with RPL19 as the covariate. Data were presented as least-square means ± SEM and differences were considered significant at p < 0.05.
The cDNA of DERL1 was cloned from bovine granulosa cells (GC) of day 5 dominant follicles and characterized by sequencing. The cDNA of DERL1 is 2617 bp in length and encodes a hydrophobic protein of about 28.8 kDa containing 251 amino acid residues (GenBank: AF279909). Amino acid analysis indicated that DERL1 presents four putative transmembrane domains located between amino acid residues F19-S35, L99-A115, L120-L136, and L155-I171, a feature of membrane protein. Sequence comparison showed orthologous proteins from human (GenBank accession number: NP_077271), pig (XP_001928865), mouse (NP_077169), chicken (NP_001006350), and frog (NP_001085401) that were, respectively, 99, 99, 97, 90, and 86% identical to bovine DERL1 (AF279909).
These results provide support for a physiologically relevant role for DERL1 in the ovary. First, the greatest expression of DERL1 is associated with active ovarian follicular growth, suggesting a role of DERL1 in developing follicles. The preovulatory LH surge induces several changes in different compartments of the ovarian follicle as it triggers ovulation, maturation of the cumulus oocyte complex, and luteinization [23–26]. In ovulatory follicles, DERL1 decreased compared to dominant follicles in our in vivo study. Following the comparison of its mRNA profile in different bovine tissues by Northern blot analysis, we hypothesized that DERL1 mRNA in granulosa cells would vary in relation to follicular development. Indeed, we have shown that DERL1 mRNA in granulosa cells is regulated depending on the developmental stage of the follicle. Increased expression of DERL1 in dominant follicles and early CL might contribute to protect granulosa cells against apoptosis and/or developmental stresses during follicular or luteal growth. Similarly, recent data in breast cancer cells showed that DERL1 expression is increased by ER stress while DERL1 knockdown resulted in decreased development of cancer cells . The latter study demonstrated that DERL1 expression might be induced by stress inducers in breast cancer cell lines and suggested that DERL1 could protect cancer cells against ER stress-induced apoptosis . These observations support a crucial role of DERL1 for survival of rapidly growing cells within normal tissues such as granulosa cells or within tumorigenic tissues.
Additional evidence for the physiological significance of DERL1 in the ovary is the differential expression of DERL1 mRNA and protein during the luteal phase, and the physical interaction of DERL1 protein with class I MHC in freshly collected luteal tissues. No report is available regarding DERL1 expression and regulation in the CL. We demonstrated a link between DERL1 expression and the early stages of the functional CL. Following ovulation, the luteinization process is realized under intense tissue remodeling and organization. This process involves repression and/or expression of several factors. The greatest expression of DERL1 early in this process is in accordance with its putative role in the quality control machinery of newly synthesized proteins. Toward the end of the estrous cycle, DERL1 mRNA and protein decreased significantly, suggesting that DERL1 might be required for the maintenance of a functional CL. Analysis of progesterone (P4) concentrations showed that the CL used at day 18 were not regressing further supporting that the decrease in DERL1 expression was a physiological event.
The exact role of DERL1 in the ovary is unknown. However, as previously hypothesized for other systems [7, 8], DERL1 proteins may form a channel by oligomerization or association with other factors, and are required for protein degradation associated with the ER. Also, it has been suggested that p97 ATPase participates in a complex with DERL1 and that, in this complex, p97 ATPase provides the energy for dislocation . In the CL, we did not find any interaction between DERL1 and p97. This may be because this interaction is triggered by a specific event in the cell such as ER stress due to accumulation of misfolded or unassembled proteins. An early step during the assembly of a retrotranslocation complex involves the targeting of a retrotranslocation polypeptide to DERL1 that is not associated with the p97 ATPase . At a later stage, the p97 ATPase joins DERL1 to form a larger retrotranslocation complex. Similarly, in the present study, no interaction was found between DERL1 and VIMP. The membrane protein VIMP was shown to interact with DERL1 and recruits the p97 ATPase and its cofactor, the UFD1/NPL4 complex, to the ER for retrotranslocation of misfolded proteins into the cytosol . The absence of interaction between DERL1 and VIMP would then explain why there is no recruitment of the p97 ATPase in luteal tissues.
Interestingly, DERL1 was found to interact with class I MHC molecules in luteal tissues used in the present study. A physiological role for MHC molecules in luteal function is supported by the fact that both class I and class II molecules are expressed by luteal cells in vivo [10–12, 30]. MHC molecules mediate the interaction between luteal cells and T lymphocytes as a form of cell-cell signaling to activate resident immune cells . The expression of MHC molecules and the recognition of antigenic peptides by MHC serve as a means to regulate T lymphocyte activation, thus controlling cytokine production. DERL1 interacts with US11, a virally-encoded ER protein that specifically targets MHC class I heavy chains for export from the ER [8, 31–33]. In the present study, normal luteal tissues, analyzed at different stages of development, showed a constant and direct interaction between DERL1 and class I MHC. As a DERL1-associated protein, class I MHC may represent a factor that targets dislocation of substrates to the multiprotein complexes. Alternatively, binding of DERL1 with class I MHC may prevent presentation of processed peptides to T lymphocytes, because DERL1 will control the integrity of class I molecules, preventing activation of T lymphocytes. Stimulation of T lymphocyte activation is dependent on the specific interaction of a T cell receptor for antigen (TCR) on the T cell with MHC molecules located on the surface of the target cell . The outcome of binding of the TCR to the MHC molecule is determined by the peptides bound to the MHC molecules. Antigenic peptides that are presented to T cells via MHC molecules are derived from proteins that are proteolytically digested into short peptides prior to binding to MHC molecules. Since antigen processing can determine the types of peptides bound to MHC molecules, this process can impact whether cells within a tissue are able to activate T lymphocytes, thereby stimulating an immune response, while binding of DERL1 to class I MHC would affect this process.
The ability of cells to dispose of misfolded proteins is critical for cell survival. The expression of DERL1 in the developing follicles and in the functional CL provides strong evidence that DERL1 might be involved in tissue remodeling events and maintenance of function. Although the exact function of DERL1 in the ovary is not fully understood, its greater expression seems to be specifically associated with active follicular growth and early CL development and function. Furthermore, the observation in yeast that deletion of Der1p, homolog of mammalian DERL1, abolished degradation of the substrate proteins  also adds more evidence that DERL1 plays a fundamental role in the cell and might be a pivotal factor in cell survival, which is consistent with its expression in growing follicles and functional CL. Finally, although the retrotranslocation pathways appear to require the function of the p97 ATPase complex , the present study found no interaction betwen p97 and DERL1 or between VIMP and DERL1. This could suggest that the binding of DERL1 to MHC I would be a regulation of MHC I molecules by DERL1 rather than a retrotranslocation process.
This project was supported by National Research Initiative Competitive Grant no. 20043520314789 from the USDA Cooperative State Research, Education, and Extension Service, Animal Reproduction Program and NIH research grant HD37550 to JLP. Salaries and research support also provided by State and Federal funds appropriated. The project was also supported by a Discovery Grant (to JGL) from the Natural Sciences and Engineering Research Council of Canada (NSERC). The authors also thank Mr. Daniel Poole for assistance in material collection and statistical analysis.
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