Effects of vitrification on nuclear maturation, ultrastructural changes and gene expression of canine oocytes
© Turathum et al; licensee BioMed Central Ltd. 2010
Received: 20 April 2010
Accepted: 22 June 2010
Published: 22 June 2010
Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of vitrification on nuclear maturation, ultrastructural changes and gene expression of canine oocytes.
Immature oocytes (germinal vesicles) isolated from ovaries of normal bitches (> 6 months of age) were either vitrified in open pulled straw (OPS) using 20% ethylene glycol (EG) and 20% dimethyl sulfoxide (DMSO) as vitrification solution or exposed to vitrification solution without subjected to liquid nitrogen. After warming, oocytes were investigated for nuclear maturation following in vitro maturation (IVM), ultrastructural changes using transmission electron microscopy (TEM) and gene expression using RT-PCR. Fresh immature oocytes were used as the control group.
The rate of resumption of meiosis in vitrified-warmed oocytes (53.4%) was significantly (P < 0.05) lower than those of control (93.8%) and exposure (91.4%) groups. However, there were no statistically significant differences among groups in the rates of GV oocytes reaching the maturation stage (metaphase II, MII). The ultrastructural alterations revealed by TEM showed that cortical granules, mitochondria, lipid droplets and smooth endoplasmic reticulum (SER) were affected by vitrification procedures. RT-PCR analysis for gene expression revealed no differences in HSP70, Dnmt1, SOD1 and BAX genes among groups, whereas Bcl2 was strongly expressed in vitrified-warmed group when compared to the control.
Immature canine oocytes were successfully cryopreserved, resumed meiosis and developed to the MII stage. The information obtained in this study is crucial for the development of an effective method to cryopreserve canine oocytes for establishment of genetic banks of endangered canid species.
A major obstacle for the development of assisted reproductive technologies in canines is the low percentage of oocytes reaching the maturation stage (i.e., metaphase II, MII) following IVM. In contrast to most of other mammals that oocytes are at the MII stage when ovulated, canine oocytes released from ovaries are at the prophase I stage of the first meiotic division and they subsequently completed nuclear maturation within 60-72 h in the oviduct . Several studies have been made to improve the rates of oocyte maturation in vitro, however, little progress has been achieved and usually less than 20% of canine oocytes complete nuclear maturation [2, 3]. Although the low efficiency of IVM of bitch oocytes remaining unresolved, the development of oocyte cryopreservation is important for establishing genetic banks as well as for developing applications for conservation of endangered canid species . The first successful IVF producing live offspring from cryopreserved mouse oocytes frozen and stored in liquid nitrogen was reported in 1977 . Subsequently, successful cryopreservation of oocytes has been achieved in other mammalian species [5, 6] including human [7, 8]. However, there is no report on the cryopreservation of canine oocytes.
Previous studies reported success in cryopreservation of bovine oocytes but mature (MII stage) oocytes were susceptible to cooling damage resulting in disruption of meiotic spindle and chromosome . Ultrastructural studies on vitrified bovine oocytes have revealed that intercellular communication between the cumulus cells and oocyte might have been interrupted and that the zona pellucida might have been modified by premature cortical granule release . Ultrastructural alterations of the cytoskeleton, mitochondria, cortical granules and nucleoli have also been observed in bovine oocytes [11, 12]. Structural changes of vitrified oocytes have also been observed in porcine  and human oocytes . Immature oocytes in which organization of the meiotic spindle did not develop may be an alternative source for genetic banks. Therefore, the aim of this study was to investigate the effects of vitrification on nuclear maturation, ultrastructural changes and gene expression on vitrified-warmed immature canine oocytes.
Chemicals and media
All chemicals in this study were purchased from Sigma Chemical Company (Sigma, St. Louise, MO, USA), unless indicated otherwise. Media was prepared once a week, filtered (0.2 μm, Sartorius, Minisart, CA, USA) and kept in sterile bottles. Synthetic oviductal fluid (SOF) cultured media was incubated at 38.5°C under 5% CO2 in air at least 4 h before use.
Collection of oocytes
Ovaries were obtained from normal bitches of various breeds at various ages (> 6 months old) by ovariohysterectomy at the veterinary clinic of the Veterinary Public Health Division, Bangkok Metropolitan Administration. Ovaries were placed in 0.9% NaCl (containing 100 IU/ml penicillin) and transported to the laboratory (at 25-32°C) within 2-4 h after removal. Ovaries were washed three times in 0.9% NaCl containing 100 IU/ml penicillin. To collect cumulus-oocyte complexes (COCs), ovaries were sliced repeatedly in Petri dishes containing TCM 199 (Invitrogen, Carlsbad, CA, USA) supplemented with 25 mM HEPES, 0.1% polyvinylalcohol, 0.1 mM glutamine, 2.5 mM sodium pyruvate and 1% penicillin-streptomycin. Cumulus-oocyte complexes were washed and graded under a stereomicroscope (200×) using criteria based on the uniformity of ooplasm and cumulus cell complement, as previously described . Grade 1 oocytes were surrounded with more than five layers of compact cumulus cells and had homogeneous dark cytoplasm. Grade 2 oocytes were surrounded by three to five layers of compact cumulus cells and had homogeneous dark cytoplasm. Grade 3 oocytes were partially surrounded by cumulus cells and lacked homogeneous cytoplasm. Grade 4 oocytes were denuded (without surrounding cumulus cells) and lacked homogeneous cytoplasm. Cumulus-oocyte complexes with more than three layers of cumulus cells with dark pigment oocyte cytoplasm (grade 1 and grade 2) were selected for the experiments.
Vitrification and warming of immature oocytes
The procedures for cryopreservation of oocytes were performed at room temperature. Immature oocytes (germinal vesicle stage) were exposed to the holding medium (HM) (TCM 199 supplemented with 20 mM Hepes and 20% FBS) for 5 min and then equilibrated in HM+4% EG for 5 min. They were exposed to HM+10% EG+10% DMSO for 1 min and then placed in 2-3 μl drop of the vitrification solution (HM+20% EG+20% DMSO+0.5 mM sucrose) for 30 sec. The COCs were then loaded in OPS straws (five oocytes per straw), plunged directly into the liquid nitrogen and stored for 7 days. Warming was done by immersing the tip of OPS straw into 1 ml of HM+0.3 mM sucrose at 37°C for 1 min. The oocytes were directly expelled into the medium after the vitrified medium became liquid. The oocytes were transferred into HM+0.15 M sucrose for further rehydration. They were then washed and incubated in holding medium for 5 min before further evaluation.
Morphological assessment of vitrified-warmed oocytes
In vitro maturation
The COCs were cultured in SOF supplemented with 40 ng/ml epidermal growth factor (EGF) . Twenty COCs were cultured for 48 h in a four-well Petri dishes containing 500 μl SOF under mineral oil in each well. The dishes were held in an incubator at 38.5°C in a humidified atmosphere of 5% CO2 in air.
Experiment I: Nuclear maturation of vitrified-warmed canine oocytes
Cumulus-oocyte complexes were divided into 3 groups: 1) control, 2) exposure; oocytes were exposed to vitrification media without loading into straws and storage in liquid nitrogen, and 3) vitrified-warmed; oocytes were vitrified, loaded into straws, stored in liquid nitrogen for 7 days and warmed at 38.5°C. Cumulus-oocyte complexes were cultured in SOF for 48 h at 38.5°C. Following 48 h culture, they were denuded, fixed and stained with Hoechst 33342. They were then evaluated for the meiotic stage under a fluorescence microscope.
Assessment of nuclear maturation
Experiment II: Ultrastructural changes of vitrified-warmed canine oocytes
Based on the results of experiment I that there were no significant differences in rates of nuclear maturation among groups the control and vitrified-warmed groups were included in this study. COCs at 0 h of IVM collected from both groups were fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4) for 120 min at 4°C and washed three times in the same buffer for 10 min at 4°C. Samples were post-fixed with 1% osmium tetraoxide in 0.1 M sodium cacodylate buffer for 1 h at 4°C. They were gradually dehydrated for 10 min twice in each concentration in series of ethanol (35, 50, 70, 80, 90, 95 and 100%) and treated twice for 10 min with propylene oxide, infiltrated with 1:1 propylene oxide/resin in embedding capsules (Electron Microscopy Sciences, Washington, PA, USA) overnight, and finally embedded in fresh resin. Thin sections (60-80 nm) were cut with an ultramicrotome and collected on glass slides or 200-mesh thin bar copper grids. Thin sections were stained with saturated uranyl acetate in 80% methanol and lead citrate. These sections were observed and photographed with a transmission electron microscope at 80 KV.
Experiment III: Gene expression of vitrified-warmed canine oocytes
Primers used for RT-PCR analysis
All oocytes were randomly distributed within each experimental group and each experiment was repeated at least three times. Data were arc-sine transformed and then subjected to ANOVA; when necessary, differences were located with Duncan's New Multiple Range Test. Differences of P < 0.05 were considered significant.
Experiment 1: The effect of vitrification on nuclear maturation of vitrified-warmed canine oocytes
Mean (± SD) nuclear status of control, exposed and vitrified-warmed canine oocytes after in vitro culture for 48 h
No. oocytes cultured
Nuclear status (%)
6.2 ± 7.4a
35.4 ± 18.0
50.1 ± 19.1
8.2 ± 3.7
93.8 ± 7.4 a
8.7 ± 10.1a
34.5 ± 14.2
50.1 ± 10.2
6.7 ± 5.0
91.4 ± 10.1 a
46.6 ± 14.8b
18.0 ± 10.0
31.5 ± 2.6
3.9 ± 2.3
53.4 ± 14.8 b
Experiment 2: Ultrastructure of control, exposed and vitrified-warmed canine oocytes
Experiment 3: Gene expression of control and vitrified-warmed canine oocytes
Successful cryopreservation of canine oocytes is a stepping stone towards applying assisted reproduction technology (ART) for conservation of endangered canids. To our knowledge, this is the first report conducted on vitrification of immature canine oocytes. Although the rate of meiotic resumption of vitrified-warmed oocytes was significantly lower than those of the control and exposure groups there was no statistically significant difference in rates of meiotic progression to the MII stage.
Various types of cryoprotectants (i.e., EG, DMSO, glycerol, propylene glycol, polyethylene glycol and 1,2-propanediol) have been used in different combinations for the vitrification of mammalian oocytes and embryos . EG has been found to be less toxic than glycerol and propylene glycol to mouse embryos . In addition, it was shown to allow much higher survival rates of bovine embryos . DMSO has been found to be an effective cryoprotectant for vitrification of mouse and hamster oocytes [19, 20]. In consequence, EG and DMSO were chosen as cryoprotectants in this present study to avoid ice formation based on the evidence of previous studies in which cryoprotection was useful for the successful vitrification of immature buffalo oocytes . The vitrification technique used high cryoprotectant concentrations, which have been described as toxic to cells . Contrarily, an appropriate phase composition of cryoprotectant mitigates the toxic and osmotic consequences of highly concentrated cryoprotectants . Thus, a mixture of cryoprotectants can decrease individual specific toxicity. In the present study, we vitrified canine oocytes in OPS using mixture of 20% EG and 20% DMSO as previously described .
Vitrification of immature bovine and equine oocytes using OPS resulting in subsequent cleavage and development to the blastocyst stage has been reported [25, 26]. The OPS method used for cryopreservation of oocytes offers many advantages over other methods. It is simple, inexpensive, achieves a great increase in the speed of cooling by reducing the volume to be vitrified and by thinning the isolating layer between the cooling agent (LN2) and the vitrification solution. A further advantage of using very small volumes of vitrified drops was a reduction of the amount of damage to the zona pellucida which occurred during cooling and warming .
Our results demonstrated that vitrified-warmed immature canine oocytes in OPS can successfully resume meiosis and develop to the MII stage following IVM. However, the efficiency of oocyte cryopreservation methods is still unsatisfactory. Vitrification procedure reduced oocyte competence to resume meiosis. The assessment of cryo-damaged processes and organelles is fundamental in the evaluation and refinement of current and future cryopreservation protocols. The ultrastructural alterations of vitrified-warmed oocytes revealed by TEM indicated that vitrification procedures affect the pericortical distribution and morphofunctional integrity of cortical granule, mitochondria, lipid droplet and SER.
Morphological examination demonstrated that cortical granules were reduced in numerical density and were damaged in vitrified-warmed oocytes, similar to results in previous studies [12, 28, 29]. Wessel et al.  reported that cortical granules are Golgi-derived, membrane-bound spherical or slightly ovoid organelles formed during the early stages of oocyte and at maturation. Cortical granules are believed to establish the block to polyspermy by preventing penetration of additional spermatozoa. Fuku et al.  showed that the numbers of cortical granules along the oolemma were substantially reduced, and fusion of cortical granules with the plasma membrane followed by exocytosis of granule core or intact cortical granule into the perivitelline space was seen in all vitrified bovine IVM oocytes. Ghetler et al.  found that the cryopreservation procedure resulted in the loss of cortical granules from the cortical area and in appearance of vesicles within the cytoplasm of both immature and mature human cryopreserved oocytes, which might indicate structural damage occurring from the freezing and warming process.
It has been suggested that the shape and intracellular distribution of mitochondria were related to the level of cell metabolism, proliferation and differentiation, and that these organelles generate the essentials required in a crucial period of the cell cycle . In addition, the present study demonstrated that mitochondria corresponding to the cortical zone were shown to increase in numerical density, to elongate, develop a coarse surface, broken and unclear, of decreased electron density. Similar findings were reported in the exposure of porcine oocytes to vitrification solutions . Roberto et al.  reported mitochondria of frozen-thawed human oocytes with decreased matrix electron density or with ruptures of the outer and inner membranes. Mitochondria were the most abundant organelles in mammalian oocytes and their dysfunction or abnormalities would critically determine oocyte and embryo developmental competence. Structural changes to lipid droplets in the present study were in agreement with previous reports in bovine and porcine oocytes [13, 31, 32]. Xiang et al.  suggested that the increased small lipid droplets came from broken larger lipid droplets and exist in the form of smaller drops during vitrification of porcine oocytes. Isachenko et al. also reported that lipid droplets in porcine oocytes changed morphologically during cooling; they changed into a spherical form with lucent streaks .
The present study showed the expression of stress genes (HSP70), Dnnt1 and SOD1 were similar in control and vitrified-warmed groups. The identical expression patterns of HSP70, Dnnt1 and SOD1 in the control and vitrified groups, indicating that the vitrification protocol did not alter the expression pattern of these stress genes. It is well known that members of Bcl2 gene family play a major role in regulation of apoptosis. In that regard, Bcl2 is anti-apoptosis and promotes cell survival, whereas BAX is pro-apoptosis and promotes cell death . Apoptosis is an underlying process in oocyte degeneration and embryo fragmentation . This experiment found that Bcl2 was strongly expressed in the vitrified-warmed group when compared to the control, whereas BAX wasn't expressed in both groups. Our results were similar to report in mouse embryos that the expression of BAX gene did not differ from control when morulae were vitrified . Contrarily, Dhali et al.  showed that Bcl2 displayed a greater decrease in the vitrified mouse embryos compared to control and expression of BAX gene was down-regulated in the treated group. The expression of Bcl2 was higher in normal than fragmented ones, and the expression did not vary significantly among embryos of varying quality . Moreover, the expression of Bcl-2 was comparatively lower in the vitrified embryos than the normal embryos . Therefore, the differential pattern of gene expression observed in the present study may be used to predict the quality and developmental ability of vitrified immature canine oocytes.
In conclusion, the present study demonstrated that it is possible to cryopreserve immature canine oocyte by vitrification using EG in combination with DMSO and modified OPS protocol. Our results showed that vitrified-warmed immature canine oocytes can resume meiosis and develop to the MII stage. Further studies are required to investigate the fertility and developmental ability of MII oocytes following fertilization.
We thank Professor LM Lewin for critical reading and editing the manuscript. This study was supported by Mahidol Research Fund, Mahidol University.
- Reynaud K, Fontbonne A, Marseloo N, Thoumire S, Chebrout M, de Lesegno CV, Chastant-Maillard S: In vivo meiotic resumption, fertilization and early embryonic development in the bitch. Reproduction. 2005, 130: 193-201. 10.1530/rep.1.00500.View ArticlePubMed
- Songsasen N, Wildt DE: Oocyte biology and challenges in developing in vitro maturation systems in the domestic dog. Anim Reprod Sci. 2007, 98: 2-22. 10.1016/j.anireprosci.2006.10.004.PubMed CentralView ArticlePubMed
- Saikhun J, Sriussadaporn S, Thongthip N, Pinyopummin A, Kitiyanant Y: Nuclear maturation and development of IVM/IVF canine embryos in synthetic oviductal fluid or in co-culture with buffalo rat liver cells. Theriogenology. 2008, 69: 1104-1110. 10.1016/j.theriogenology.2008.01.024.View ArticlePubMed
- Whinttingham DG: Fertilization in vitro and development to term of unfertilized mouse oocyte previously stored at -196 degrees C. J Reprod Fertil. 1977, 49: 89-94. 10.1530/jrf.0.0490089.View Article
- Al-Hasani S, Kirsch J, Diedrich K, Blanke S, van der Ven H, Krebs D: Successful embryo transfer of cryopreserved and in-vitro fertilized rabbit oocytes. Hum Reprod. 1989, 4: 77-79.PubMed
- Vieira AD, Mezzalira A, Barbieri DP, Lehmkuhl RC, Rubin MIB, Vajta G: Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology. 2002, 45: 91-94. 10.1016/S0011-2240(02)00109-8.View ArticlePubMed
- Chen C: Pregnancy after human oocyte cryopreservation. Lancet. 1986, 2: 884-886. 10.1016/S0140-6736(86)90989-X.View Article
- Tao T, Valle AD: Human oocyte and ovarian tissue cryopreservation and its application. J Assist Reprod Genet. 2008, 25: 287-296. 10.1007/s10815-008-9236-z.PubMed CentralView ArticlePubMed
- Martino A, Songsasen N, Leibo SP: Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol Reprod. 1996, 54: 1059-1069. 10.1095/biolreprod54.5.1059.View ArticlePubMed
- Fuku E, Xia L, Downey BR: Ultrastructural changes in bovine oocytes cryopreserved by vitrification. Cryobiology. 1995, 32: 139-156. 10.1006/cryo.1995.1013.View ArticlePubMed
- Wu B, Tong J, Leibo SP: Effects of cooling germinal vesicle-stage bovine oocytes on meiotic spindle formation following in vitro fertilization. Mol Reprod Dev. 1999, 54: 388-895. 10.1002/(SICI)1098-2795(199912)54:4<388::AID-MRD9>3.0.CO;2-7.View ArticlePubMed
- Hyttel P, Vajta G, Callesen H: Vitrification of bovine oocytes with open pulled straw method: ultrastructural consequences. Mol Reprod Dev. 2000, 56: 80-88. 10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>3.0.CO;2-U.View ArticlePubMed
- Wu C, Rui R, Dai J, Zhang C, Ju S, Xie B, Lu X, Zheng X: Effects of cryopreservation on the developmental competence, ultrastructure and cytoskeletal structure of porcine oocytes. Mol Reprod Dev. 2006, 73: 1454-1462. 10.1002/mrd.20579.View ArticlePubMed
- Roberto G, Mirella I, Valetina M, Marina P: Slow cooling of human oocytes: ultrastructural injuries and apoptotic status. Fertil Steril. 2009, 91: 1023-1234. 10.1016/j.fertnstert.2008.01.076.View Article
- de los Reyes M, de Lange J, Miranda P, Palominos J, Barros C: Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes. Theriogenology. 2005, 64: 1-11. 10.1016/j.theriogenology.2004.11.008.View ArticlePubMed
- Palasz AT, Mapletoft RJ: Cryopreservation of mammalian embryos and oocytes: recent advances. Biotechnol Adv. 1996, 14: 127-149. 10.1016/0734-9750(96)00005-5.View ArticlePubMed
- Kasai M, Komi JH, Takakamo K, Isudera H, Sakurai T, Machida T: A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil. 1990, 89: 91-97. 10.1530/jrf.0.0890091.View ArticlePubMed
- Mahmoudzadeh AR, Van Soom A, Van Vlaenderen I, De Kruif A: A comparative study of the effect of one-step addition of different vitrification solutions on in vitro survival of vitrified bovine embryos. Theriogenology. 1993, 39: 1291-1302. 10.1016/0093-691X(93)90231-S.View Article
- Bos-Mikich A, Wood MJ, Candy CJ, Whittingham DG: Cytogenetical analysis and developmental potential of vitrified mouse oocytes. Biol Reprod. 1995, 53: 780-785. 10.1095/biolreprod53.4.780.View ArticlePubMed
- Wood MJ, Barros C, Candy CJ, Carroll J, Melendez J, Whittingham DG: High rates of survival and fertilization of mouse and hamster oocytes after vitrification in dimethyl sulphoxide. Biol Reprod. 1993, 49: 489-495. 10.1095/biolreprod49.3.489.View ArticlePubMed
- Dhali A, Manik RS, Singla SK, Palta P: Vitrification of buffalo (bubalus bubalis) oocytes. Theriogenology. 2000, 53: 1295-1303. 10.1016/S0093-691X(00)00273-9.View ArticlePubMed
- Fuller B, Paynter S: Fundamentals of cryobiology in reproductive medicine. Reprod Biomed Online. 2004, 6: 680-691. 10.1016/S1472-6483(10)61780-4.View Article
- Vajta G, Nagy ZP: Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod Biomed Online. 2006, 12: 779-796. 10.1016/S1472-6483(10)61091-7.View ArticlePubMed
- Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H: Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev. 1998, 51: 53-58. 10.1002/(SICI)1098-2795(199809)51:1<53::AID-MRD6>3.0.CO;2-V.View ArticlePubMed
- Vieira AD, Forell F, Feltrin C, Rodrigues JL: Calves born after direct transfer of vitrified bovine in vitro-produced blastocysts derived from vitrified immature oocytes. Reprod Domest Anim. 2008, 43: 314-318. 10.1111/j.1439-0531.2007.00899.x.View ArticlePubMed
- Hurtt AE, Landim-Alvarenga F, Seidel GE, Squires EL: Vitrification of immature and mature equine and bovine oocytes in an ethylene glycol, ficoll and sucrose solution using open-pulled straws. Thriogenology. 2000, 54: 119-128. 10.1016/S0093-691X(00)00330-7.View Article
- Lj X, Su L, Li Y, Ji W, Dinnyés A: Vitrification of Yunnan Yellow cattle oocytes: work in progress. Theriogenology. 2002, 58: 1253-1260. 10.1016/S0093-691X(02)00954-8.View ArticlePubMed
- Fuku E, Xia L, Downey BR: Ultrastructural changes in bovine oocytes cryopreserved by vitrification. Cryobiology. 1995, 32: 139-156. 10.1006/cryo.1995.1013.View ArticlePubMed
- Ghetler Y, Skutelsky E, Nun BI, Dor LB, Amihai D, Shalgi R: Human cryopreservation and the fate of cortical granules. Fertil Steril. 2006, 86: 210-216. 10.1016/j.fertnstert.2005.12.061.View ArticlePubMed
- Wessel GM, Brooks JM, Green E, Haley S, Voronina E, Wong J, Zaydfudim V, Conner S: The biology of cortical granules. Int Rev Cytol. 2001, 209: 117-206. full_text.View ArticlePubMed
- Isachenko V, Soler C, Isachenko E, Perez-Sanchez F, Grishchenko V: Vitrification of immature porcine oocytes: effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant. Cryobiology. 1998, 36: 250-253. 10.1006/cryo.1998.2079.View ArticlePubMed
- Isachenko E, Isachenko V, Michelman HW, Alabart JL, Vazquez I, Bezugly N, Nawroth F: Lipolysis and ultrastructural changes of intracellular lipid vesicles after cooling of bovine and porcine GV-oocytes. Anat Histol Embryol. 2001, 30: 333-338. 10.1111/j.1439-0264.2001.t01-1-0310.x.View ArticlePubMed
- Yang MY, Rajamahendran R: Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro. Anim Reprod Sci. 2002, 70: 159-169. 10.1016/S0378-4320(01)00186-5.View ArticlePubMed
- Brenner CA, Exley GE, Alikani M, Cohen J, McElhinny AS, Stachecki JJ, Scott RT, Warner CM: Apoptosis and human embryo survival. Fertil Steril. 1997, 68: S86-10.1016/S0015-0282(97)90806-2.View Article
- Dhali A, Anchumparuthy VM, Butler SP, Pearson RE, Mullarky IK, Gwazdauskas FC: Effect of droplet vitrification on development competence actin cytoskeletal integrity and gene expression in in vitro cultured mouse embryos. Theriogenology. 2009, 71: 1408-1416. 10.1016/j.theriogenology.2009.01.011.View ArticlePubMed
- Dhali A, Anchumparuthy VM, butler SP, Pearson RE, Mullarky IK, Gwazdauskas FC: Gene expression and development of mouse zygotes following droplet vitrification. Theriogenology. 2007, 68: 1292-1298. 10.1016/j.theriogenology.2007.08.030.View ArticlePubMed
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