Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis
© Locci et al.; licensee BioMed Central Ltd. 2013
Received: 29 July 2013
Accepted: 23 September 2013
Published: 26 September 2013
Endometrial cells, which are shed by retrograde menstruation, may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of the laminin gamma 2 chain (LAMC2) in the tissues of women with and without endometriosis.
Endometrial biopsy specimens were collected from healthy volunteers and from endometriosis patients. Biopsy specimens from the corresponding endometriotic lesions were also collected. The expression of laminin gamma 2 chain was evaluated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).
Endometrial tissue from women with or without endometriosis showed constitutive expression of LAMC2 mRNA throughout the menstrual cycle. A higher mRNA level was observed in ectopic endometrium (Ec) from women with endometriosis compared with eutopic endometrium (Eu) from women with endometriosis. Immunohistochemistry revealed a varied pattern of laminin gamma 2 chain expression, with increased epithelial expression in eutopic endometrium from women with endometriosis compared with those without endometriosis.
The altered expression of laminin gamma 2 chain in eutopic endometrium from women with endometriosis may provide new opportunities for diagnosis and treatment.
KeywordsEndometrium Endometriosis Cell invasion Adhesion
Endometriosis is a common benign, hormone-dependent gynaecological disease that is characterised by the presence and growth of endometrial tissue outside the uterus. The most widely accepted theory is that retrograde menstruation through the fallopian tube leads to the transfer of endometrial cells into the peritoneal cavity, where they become embedded in the pelvic structures  (for a review, see ). However, this theory does not explain why more than 80% of women of reproductive age experience retrograde menstrual bleeding but do not develop endometriosis. Currently, a combination of many theories, including immunological defects, genetic predisposition and epigenetic modifications, could provide possible explanations as regarding the cause of the disease. Nevertheless, the adhesion, invasion and proliferation of ectopic endometriotic cells are all necessary for the establishment of endometriotic lesions. Notably, endometriotic cells are histologically benign but display invasive characteristics.
Laminin-332 (LN-332, previously termed laminin-5), also referred to as kalinin, nicein and epiligrin, consists of alpha 3, beta 3 and gamma 2 chains, which represent the products of three distinct genes (LAMA3, LAMB3 and LAMC2, respectively). LN-332 is a laminin isoform that is a major adhesive component of epidermal basement membranes [3, 4]. In vitro, LN-332 promotes the attachment, spreading, scattering and migration of non-tumorigenic epithelial cells [5, 6]. LN-332 also stimulates human tumour cells to form lamellipodia, leading to enhanced cell migration and invasion . Immunohistochemical studies have shown that LN-332 (or its subunits) is highly expressed in various types of human cancers. In particular, the laminin gamma 2 chain is expressed in tumour cells at the invasion front or in budding tumour cells in many types of human cancers such as adenocarcinoma of the colon, breast, pancreas and lung, squamous cell carcinoma and melanoma [8, 9].
Because endometriosis is characterised by the acquisition of malignant properties, such as the ability to invade surrounding tissue and disseminate to ectopic sites, the aim of the present study was to investigate the expression of the laminin gamma 2 chain in the tissues of women with and without endometriosis.
N° of patients
N° of patients with one lesion
N° of patients with more than one lesion
All endometrial biopsy samples were obtained with a Cornier Pipelle suction curette (C.C.D. International, Paris, France), which allows sampling of the functional layer of the endometrium. All samples were classified according to classical histologic criteria .
Patients provided informed consent, and the Institutional Review Board of the University of Liège approved the collection and use of human tissue.
Reverse transcription-polymerase chain reaction (RT-PCR) analysis
For gene expression analysis, endometrial biopsy specimens were collected from healthy volunteers (EuE-: proliferative phase, n = 12; secretory phase, n = 15) and from endometriosis patients (EuE + and Ec, n = 9) (Table 1). After surgical resection, the tissue samples were immediately frozen in liquid nitrogen. The frozen tissues were processed as previously described . The specific primers (Eurogentec, Liège, Belgium) for LAMC2 mRNA were 5′-AAAGCCACGTTGAGTCAGC-3′ (forward) and 5′-TCTTCCACCTGAAAGGACTGAT-3′ (reverse). The specific primers for 28S rRNA were 5′-GTTCACCCACTAATAGGGAACGTGA-3′ (forward) and 5′-GGATTCTGACTTAGAGGCGTTCAGT-3′ (reverse). RT-PCR was performed using 10-ng aliquots of cDNA, Taq polymerase (Takara, Shiga, Japan) and 5 pmol of each primer. The specific PCR products were resolved on 10% polyacrylamide gels (Bio-Rad) and analysed with a luminescent image analyser (LAS-4000, Fujifilm) after GelStar staining (Lonza Rockland, Inc., Rockland, ME). The LAMC2 mRNA levels were expressed as ratios of the 28S rRNA as previously reported .
Tissue samples were fixed in 4% formalin for 4–12 hours, embedded in paraffin and cut into 4-μm sections. The sections were mounted on SuperFrost Plus glass slides (Menzel-Gläser, Braunschweig, Germany), dewaxed in xylene, rehydrated and subsequently autoclaved for 11 min at 126°C and 1.4 bar in Target Retrieval Buffer (S2031 for laminin gamma 2 chain; DakoCytomation, Glostrup, Denmark) or treated with proteinase K (S3004 for cytokeratin 7 and S1699 for Ki-67; DakoCytomation, Glostrup, Denmark). Endogenous peroxidases were blocked by treatment with 3% H2O2/H2O for 20 min, and non-specific binding was prevented by incubation in Universal Blocking Reagent (BioGenex, San Ramon, CA, USA) for 3 min. The sections were incubated with the following primary antibodies: laminin gamma 2 chain (Dako, M7262, diluted 1:25), cytokeratin 7 (BD Biosciences, 345779, ready-to-use) and Ki-67 (Dako, M7240, diluted 1:100). The sections were washed in PBS and subsequently incubated for 30 min with EnVision + HRP (K4001, Dako) or biotinylated goat anti-mouse antibodies (Dako E0433, diluted 1:400) followed by incubation with peroxidase-labelled streptavidin for 30 min (Dako P0397, diluted 1:500). Staining was detected with 3,3′-diaminobenzidine (DAB) chromogen. The nuclei were counterstained by incubation with haematoxylin for 2 min. The sections were mounted, examined and photographed. The negative control samples were processed by omitting the primary antibody or by incubating the sections with nonspecific IgG at the same concentration as the primary antibody. Placenta was used as a positive control.
Immunohistochemical staining analysis was semi-quantitative. The intensity of staining was characterised as follows: no staining (0), weak but detectable (1), strong (2) or very strong (3). The percentage of positive glands was graded as follows: no positive glands (0), less than 11% (1), 11-50% (2), 51-80% (3) or greater than 80% (4). The final score was calculated by multiplying the two scores.
The patient groups were compared using the Kruskal-Wallis test, and significant differences were further analysed via pairwise comparisons using the Mann–Whitney test. The results are presented as medians ± quartiles (25th-75th percentile). P values < 0.05 were considered statistically significant.
Laminin gamma 2 mRNA expression
Laminin gamma 2 immunoreactivity in ectopic endometrium
Laminin gamma 2 immunoreactivity in eutopic endometrium from women with and without endometriosis
Positive staining for the laminin gamma 2 chain was observed in epithelial basement membranes around individual glands and in the basement membranes underlying the endometrial surface epithelium (Figure 3D-F) in the eutopic endometrium of women with endometriosis. In normal eutopic endometrium, a similar cytoplasmic expression pattern was observed in the glandular epithelium; however, in a few cases, stronger expression was observed in the apical region of the epithelium (Figure 3G-I). Laminin gamma 2 was not observed in the stromal cells. There was no significant variation in immunoreactivity between the different menstrual phases.
Semi-quantitative evaluation of laminin gamma 2
In this study, LAMC2 mRNA was found to be differentially expressed in the ectopic endometrium of women with endometriosis compared with their eutopic endometrium (Figure 1B). The role of the laminin gamma 2 chain in the pathogenesis of endometriosis has not been previously evaluated, although some studies have implicated this protein in cancer invasion and metastasis. Therefore, we hypothesised that laminin gamma 2 chain could also play a role in the adhesion, migration and invasion of endometrial cells, which are required for the development of endometriosis . Laminin was previously found to be expressed in the glands and stroma of eutopic and ectopic endometrium however, the type of laminin investigated was unclear [14, 15]. Recently, altered expression of the LAMC1 gene was described in the endometrium of patients with endometriosis (compared with healthy endometrium) . The specific expression of the laminin gamma 2 chain has not been evaluated in human endometriosis; however, the laminin gamma 2 chain was recently described as being strongly associated with the initiation of endometriosis in a mouse model .
Endometriosis is a benign disease, although cells from endometriotic tissue and cancer cells share the ability to spread into and invade adjacent tissue. The molecular mechanisms that drive endometriosis cells to target other tissues are largely unknown. Our present data suggest that the laminin gamma 2 chain could be involved in the invasive activity of endometriotic cells as it has been found in the majority of ectopic endometrial glands.
The comparison of laminin gamma 2 chain expression in eutopic endometrium from patients without or with endometriosis also revealed significant differences (Figure 4C). The global expression score was significantly lower in eutopic endometrium from patients without endometriosis due to the number of positive glands and their intensity. Indeed, the eutopic endometrium of women without endometriosis more often displayed weaker glandular expression of the laminin gamma 2 chain. However, when analysing LAMC2 mRNA levels, we did not observe differences between eutopic endometrium from women with and without endometriosis (Figure 1C). One possible explanation for this result could be that the mRNA analysis was performed only on a limited number of RNA samples from the eutopic endometrium of women suffering from endometriosis (n = 9), whereas for protein analysis, a greater number of patients were analysed (n = 25). The differences between gene and protein expression could also be explained by post-transcriptional modification of mRNA and mature protein as well as protein degradation [18, 19]. Alternatively, small non-coding RNAs such as miRNAs, which are essentially translational repressors, could be involved in this process [20, 21]. Their absence in some physiological or pathogenic conditions can contribute to increases in the amount of protein translated from a given target mRNA without altering the amount of RNA.
The facts that the laminin gamma 2 chain was expressed unevenly and that its expression was interrupted at some points could facilitate epithelial cell motility. Our results are in agreement with a previous study showing that the laminin gamma 2 chain and the alpha 3 beta 1 integrin receptor could be involved in the mechanism of endometriosis .
Numerous studies using immunohistochemistry and in situ hybridisation have shown that the laminin gamma 2 chain is localised at the leading edge of invading carcinomas and that its expression is positively correlated with invasiveness and patient survival . However, other studies have shown that the expression of LN-332 is reduced during the progression of human carcinomas, and its expression is associated with lower invasive and metastatic activity [24, 25]. This discordance can be explained by the overexpression of the laminin gamma 2 chain monomer in tumour cells, as the laminin alpha 3 and/or beta 3 chains are often decreased or impaired in these cells [26–28]. We have previously shown that the acquisition of a migratory phenotype in epithelial cells in vitro is associated with the overexpression of MT1-MMP, which can participate in the pericellular degradation of the laminin gamma 2 chain monomer deposited by the migratory cells themselves, thereby providing a modified substrate that promotes cell migration . Differential laminin gamma 2 chain localisation and expression levels have been shown to be of prognostic value in colorectal , pancreatic  and lung adenocarcinomas  as well as gastric cancer . The serum concentrations of laminin gamma 2 fragments are also useful for assessing the treatment results and clinical courses of patients with head and neck squamous cell carcinoma . A weakness of our study is that sera from patients with and without endometriosis were not collected with the tissue samples.
In conclusion, our study showed that the laminin gamma 2 chain is a normal component of the eutopic endometrium of women with and without endometriosis. The increased expression of the laminin gamma 2 chain in eutopic endometrium from women with endometriosis suggests a possible role for this protein in endometrial cell adhesion and, consequently, in the development of endometriosis. Laminin gamma 2 chain expression by normal endometrial cells during retrograde menstruation could contribute to their peritoneal anchoring.
Although the underlying mechanisms that lead to the development of endometriosis are not fully understood, our data indicate that the glandular cells in eutopic endometrium may phenotypically differ between women with endometriosis and disease-free women.
The altered expression of laminin gamma 2 chain in eutopic endometrium from women with endometriosis might provide new opportunities for diagnosis and treatment in the future.
The authors thank M-R. Pignon, P Gavitelli, I. Dasoul, E. Feyereisen and N. Lefin for their excellent technical assistance. C.M. is Research Associate from the F.R.S.-FNRS (Belgium). This study was supported by grants from the “Fonds de la Recherche Scientifique (F.R.S.-FNRS, Belgium), the Interuniversity Attraction Poles Programme - Belgian Science Policy (Brussels, Belgium), the “Fonds Spéciaux de la Recherche” (University of Liège, Belgium) and the “Fonds Léon Fredericq” (University of Liège, Belgium).
- Bulun SE: Endometriosis. N Engl J Med. 2009, 360: 268-279. 10.1056/NEJMra0804690.View ArticlePubMedGoogle Scholar
- Giudice LC, Kao LC: Endometriosis. Lancet. 2004, 364: 1789-1799. 10.1016/S0140-6736(04)17403-5.View ArticlePubMedGoogle Scholar
- Rousselle P, Lunstrum G, Keene D, Burgeson R: Kalinin: an epithelium-specific basement membrane adhesion molecule that is a component of anchoring filaments. J Cell Biol. 1991, 114: 567-576. 10.1083/jcb.114.3.567.View ArticlePubMedGoogle Scholar
- Carter WG, Ryan MC, Gahr PJ: Epiligrin, a New cell adhesion ligand for integrin alpha 3 beta 1 in epithelial basement membranes. Cell. 1991, 65: 599-610. 10.1016/0092-8674(91)90092-D.View ArticlePubMedGoogle Scholar
- Kikkawa Y, Umeda M, Miyazaki K: Marked Stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. J Biochem. 1994, 116: 862-869.PubMedGoogle Scholar
- Rousselle P, Aumailley M: Kalinin Is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and Has a different requirement for integrin receptors. J Cell Biol. 1994, 125: 205-214. 10.1083/jcb.125.1.205.View ArticlePubMedGoogle Scholar
- Fukushima Y, Ohnishi T, Arita N, Hayakawa T, Sekiguchi K: Integrin Alpha3beta1-mediated interaction with laminin-5 stimulates adhesion, migration and invasion of malignant glioma cells. Int J Cancer. 1998, 76: 63-72. 10.1002/(SICI)1097-0215(19980330)76:1<63::AID-IJC11>3.0.CO;2-H.View ArticlePubMedGoogle Scholar
- Pyke C, Salo S, Ralfkiaer E, Romer J, Dano K, Tryggvason K: Laminin-5 Is a marker of invading cancer cells in some human carcinomas and is co expressed with the receptor for urokinase plasminogen activator in budding cancer cells in colon adenocarcinomas. Cancer Res. 1995, 55: 4132-4139.PubMedGoogle Scholar
- Sordat I, Bosman F, Dorta G, Rousselle P, Aberdam D, Blum A, Sordat B: Differential expression of laminin-5 subunits and integrin receptors in human colorectal neoplasia. J Pathol. 1998, 185: 44-52. 10.1002/(SICI)1096-9896(199805)185:1<44::AID-PATH46>3.0.CO;2-A.View ArticlePubMedGoogle Scholar
- Noyes RW, Hertig AT, Rock J: Dating the endometrial biopsy. Am J Obstet Gynecol. 1975, 122: 262-263.PubMedGoogle Scholar
- Tsatsaris V, Goffin F, Munaut C, Brichant J, Pignon M, Noel A, Schaaps J, Cabrol D, Frankenne F, Foidart J: Overexpression of the soluble vascular endothelial growth factor receptor in preeclamptic patients: pathophysiological consequences. J Clin Endocrinol Metab. 2003, 88: 5555-5563. 10.1210/jc.2003-030528.View ArticlePubMedGoogle Scholar
- Goffin F, Munaut C, Frankenne F, Perrier D’hauterive S, Beliard A, Fridman V, Nervo P, Colige A, Foidart J: Expression pattern of metalloproteinases and tissue inhibitors of matrix-metalloproteinases in cycling human endometrium. Biol Reprod. 2003, 69: 976-984. 10.1095/biolreprod.103.015933.View ArticlePubMedGoogle Scholar
- Nisolle M, Alvarez M, Colombo M, Foidart J: Pathogenesis of endometriosis. Gynecol Obstet Fertil. 2007, 35: 898-903. 10.1016/j.gyobfe.2007.07.021.View ArticlePubMedGoogle Scholar
- Beliard A, Donnez J, Nisolle M, Foidart J: Localization of laminin, fibronectin, E-cadherin, and integrins in endometrium and endometriosis. Fertil Steril. 1997, 67: 266-272. 10.1016/S0015-0282(97)81909-7.View ArticlePubMedGoogle Scholar
- Harrington D, Lessey B, Rai V, Bergqvist A, Kennedy S, Manek S, Barlow D, Mardon H: Tenascin is differentially expressed in endometrium and endometriosis. J Pathol. 1999, 187: 242-248. 10.1002/(SICI)1096-9896(199901)187:2<242::AID-PATH221>3.0.CO;2-T.View ArticlePubMedGoogle Scholar
- Sundqvist J, Andersson K, Scarselli G, Gemzell-Danielsson K, Lalitkumar P: Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: a link to the aetiology of endometriosis. Hum Reprod. 2012, 27: 2737-2746. 10.1093/humrep/des220.View ArticlePubMedGoogle Scholar
- Umezawa M, Saito Y, Tanaka-Hattori N, Takeda K, Ihara T, Sugamata M: Expression profile of extracellular matrix and adhesion molecules in the development of endometriosis in a mouse model. Reprod Sci. 2012, 19: 1365-1372. 10.1177/1933719112450340.View ArticlePubMedGoogle Scholar
- Greenbaum D, Colangelo C, Williams K, Gerstein M: Comparing Protein abundance and mRNA expression levels on a genomic scale. Genome Biol. 2003, 4: 117-10.1186/gb-2003-4-9-117.PubMed CentralView ArticlePubMedGoogle Scholar
- Vogel C, Marcotte E: Insights Into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat Rev Genet. 2012, 13: 227-232.PubMed CentralPubMedGoogle Scholar
- Pan Q, Luo X, Toloubeydokhti T, Chegini N: The Expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression. Mol Hum Reprod. 2007, 13: 797-806. 10.1093/molehr/gam063.View ArticlePubMedGoogle Scholar
- Gilabert-Estelles J, Braza-Boils A, Ramon L, Zorio E, Medina P, Espana F, Estelles A: Role Of microRNAs in gynecological pathology. Curr Med Chem. 2012, 19: 2406-2413. 10.2174/092986712800269362.View ArticlePubMedGoogle Scholar
- Giannelli G, Sgarra C, Di Naro E, Lavopa C, Angelotti U, Tartagni M, Simone O, Trerotoli P, Antonaci S, Loverro G: Endometriosis is characterized by an impaired localization of laminin-5 and alpha3 beta1 integrin receptor. Int J Gynecol Cancer. 2007, 17: 242-247. 10.1111/j.1525-1438.2006.00750.x.View ArticlePubMedGoogle Scholar
- Katayama M, Sekiguchi K: Laminin-5 in epithelial tumour invasion. J Mol Histol. 2004, 35: 277-286.View ArticlePubMedGoogle Scholar
- Martin K, Kwan C, Nagasaki K, Zhang X, O’hare M, Kaelin C, Burgeson R, Pardee A, Sager R: Down-regulation of laminin-5 in breast carcinoma cells. Mol Med. 1998, 4: 602-613.PubMed CentralPubMedGoogle Scholar
- Soini Y, Maatta M, Salo S, Tryggvason K, Autio-Harmainen H: Expression of the laminin gamma 2 chain in pancreatic adenocarcinoma. J Pathol. 1996, 180: 290-294. 10.1002/(SICI)1096-9896(199611)180:3<290::AID-PATH661>3.0.CO;2-6.View ArticlePubMedGoogle Scholar
- Koshikawa N, Moriyama K, Takamura H, Mizushima H, Nagashima Y, Yanoma S, Miyazaki K: Overexpression of laminin gamma 2 chain monomer in invading gastric carcinoma cells. Cancer Res. 1999, 59: 5596-5601.PubMedGoogle Scholar
- Kagesato Y, Mizushima H, Koshikawa N, Kitamura H, Hayashi H, Ogawa N, Tsukuda M, Miyazaki K: Sole Expression of laminin gamma 2 chain in invading tumor cells and its association with stromal fibrosis in lung adenocarcinomas. Jpn J Cancer Res. 2001, 92: 184-192. 10.1111/j.1349-7006.2001.tb01081.x.View ArticlePubMedGoogle Scholar
- Giannelli G, Fransvea E, Bergamini C, Marinosci F, Antonaci S: Laminin-5 chains are expressed differentially in metastatic and nonmetastatic hepatocellular carcinoma. Clin Cancer Res. 2003, 9: 3684-3691.PubMedGoogle Scholar
- Gilles C, Polette M, Coraux C, Tournier J, Meneguzzi G, Munaut C, Volders L, Rousselle P, Birembaut P, Foidart J: Contribution of MT1-MMP and of human laminin-5 gamma 2 chain degradation to mammary epithelial cell migration. J Cell Sci. 2001, 114: 2967-2976.PubMed CentralPubMedGoogle Scholar
- Kuratomi Y, Sato S, Monji M, Shimazu R, Tanaka G, Yokogawa K, Inoue A, Inokuchi A, Katayama M: Serum concentrations of laminin gamma 2 fragments in patients with head and neck squamous cell carcinoma. Head Neck. 2008, 30: 1058-1063. 10.1002/hed.20838.View ArticlePubMedGoogle Scholar
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