Advancements in fertility preservation strategies for pediatric male cancer patients: a review of cryopreservation and transplantation of immature testicular tissue

Table 2 Summary of autologous testicular tissue grafting in NHPs

Reference	Testicular Tissue Donor	Grafting Size	Grafting Site	Cryopreserved or Fresh	Outcome
Wistuba et al. [45] 2006	4-week-old marmosets	0.5 to 1 mm³	Back skin	Fresh	Graft survival: 31.25%. Spermatogenesis was blocked at early meiosis
Luetjen et al. [46] 2008	Prepubertal and adult marmosets	0.5 to 1 mm³	(1) Back skin	Frozen/Thawed and fresh	Graft survival: 55.7%
Luetjen et al. [46] 2008	Prepubertal and adult marmosets	0.5 to 1 mm³	(2) Scrotal skin	Frozen/Thawed and fresh	Grafts in the scrotum exhibited complete spermatogenesis
Jahnukainen et al. [47] 2012	Pubertal rhesus monkeys	1 mm³	(1) Scrotal	Frozen/Thawed	Graft survival: 7% from scrotal, 4% from shoulder and 3% from arm
			(2) Shoulder		Spermatozoa was detected in the scrotal group
			(3) Arm		Spermatozoa was detected in the scrotal group
Fayomi et al. [11] 2019	Prepubertal rhesus monkeys	9 to 20 mm³	(1) Back skin	Frozen/Thawed and fresh	Graft survival: 100%. All grafts exhibited complete spermatogenesis and produced a healthy baby
Fayomi et al. [11] 2019	Prepubertal rhesus monkeys	9 to 20 mm³	(2) Scrotal skin	Frozen/Thawed and fresh

It demonstrates enhanced graft survival when autologous testicular tissue grafting is performed in NHPs using larger graft sizes ranging from 9 to 20 mm³

ISSN: 1477-7827