Fig. 4

Transcriptome sequencing and bioinformatics analysis of three groups of mice with different treatments. (A): Volcanic maps of differentially expressed genes in the cisplatin and control groups and the Cisplatin and Cisplatin + LIPUS groups. Green indicates downregulated genes, and red indicates upregulated genes. (B): Venn diagram of different groups. (C): Heatmap. Different coloured areas represent different clustering group information. The Log10 (FPKM + 1) value was used for clustering, with red indicating high expression genes and green indicating low expression genes. The colours ranged from green to red, thus indicating higher gene expression. (D): GO analysis bar chart. GO enrichment P value histogram of differentially expressed genes in the Control vs Cisplatin groups and the Cisplatin vs Cisplatin + LIPUS groups. The ordinate is the enrichment GO term, and the abscise is the term-log10 (P value) value; n ≥ 3