Table 1 Characteristics of the ELISAs used in experiments
From: The adipokines progranulin and omentin – novel regulators of basic ovarian cell functions
Substance assayed | Specificity of assay (cross-reactivity of antiserum) | Sensitivity of assay (ng/ml) | Coefficient of variation (%) | |
|---|---|---|---|---|
Intra-assay | Inter-assay | |||
Progesterone | ≤1.1% with 11-desoxycorticosterone, ≤0.35% with pregnenolone, ≤0.30% 17α-OH with progesterone, ≤0.20% with corticosterone, ˂0.10% with estriol, 17β-estradiol, testosterone, cortisone and 11-desoxycortisol, ˂0.02% with DHEA-S and cortisol | 0.045 | 5.4 | 5.59 |
17β-estradiol | ≤9.5% with fulvestrant, ≤4.2% with estrone, ≤3.8% with E2-3-glucuronide, ≤3.6% with E2-3-sulphate, ≤0.4% with estriol, ˂0.1% with androstenedione, 17-hydroxyprogesterone, corticosterone, pregnenolone, E2-17-glucuronide, progesterone, and testosterone | 0.0062 | 6.4 | 4.5 |