Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes

Table 7 Effect of melatonin on mitochondrial distribution and intensity of in vitro matured and vitrified-warmed in vitro matured buffalo oocytes

Media	No. of oocytes	Diffused No. (%)	semi-diffused No. (%)	Peripheral No. (%)	Semi-peripheral No. (%)	Central No. (%)	X²(p)
TCM	30	21 (70%)^c,^***	6 (20%)^a,**	0 (0%)^d, *	0 (0%)^c,*	3 (10%)^b,^**	185.65 (0.00)
TCM + Mel	30	24 (80%)^a^, ***	0 (0%)^b,*	0 (0%)^d,^*	0 (0%)^c, *	6 (20%)^a,**
VTCM	30	0 (0%)^d, *	0 (0%)^b,^*	9 (30%)^b, **	21 (70%)^a, ***	0 (0%)^d
VTCM + Mel	30	6 (20%)^d, ***	0 (0%)^b	18 (60%)^a^,^**^	4 (13.3%)^b, **	2 (6.7%)^c,^*
VS2 + Mel	30	23 (76.7%)^b, ***	0 (0%)^b, *	7 (23.3%)^c,**	0 (0%)^c,*	0 (0%)^d, *

X² chi-square value, p p value
^****, ***, **, *Subscript significance difference within the same raw (P < 0.01)
^a,^b,c,dMeans denoted within the same column with different superscripts are significantly different (P ≤ 0.01)

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