Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes

Table 5 Types of abnormalities of vitrified/warmed in-vitro matured buffalo oocytes

Media	No. of abnormal oocytes	Leakage of cellular content N (%)	Cracking of zona pellucida N (%)	Shrinkage of cytoplasm N (%)	Fragmented N (%)	Zona rapture N (%)	X²(p)
VTCM	54	12 (22.22%)^b**	14 (25.93%)^a**	15 (27.78%)^b**	5 (9.26%)^b*	8 (14.81%)^a*	22.41 (0.01)
VTCM + Mel	48	15 (31.25%)^a**	5 (10.42%)^c*	12 (25%)^b**	12 (25%)^a**	4 (8.33%)^b*
VS2 + Mel	26	6 (23.1%)^b**	5 (19.23%)^b**	10 (38.45%)^a***	3 (11.53%)^b*	2 (7.69%)^b*

X² chi-square value, p p value for comparing between vitrified groups
^{***, **, *} Subscript significance difference within the same raw (P < 0.01)
^a,^b,c Means denoted within the same column with different superscripts are significantly different at (P < 0.01)

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