Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes

Table 2 Effect of melatonin addition to maturation media on nuclear maturation rate of buffalo oocytes

Media	No. oocytes	1^stpolar body			Without polar body			Degenerated
Media	No. oocytes	No.	Mean ± SE	%	No.	Mean ± SE	%	No.	Mean ± SE	%
TCM	289	199	39.8 ± 2.03	68.85 ± 1.15^b	41	8.20 ± 1.06	14.18 ± 1.35^a	49	9.80 ± 0.66	16.95 ± 0.31^a
TCM + Mel	234	200	40 ± 4.95	85.47 ± 1.62^a	16	3.20 ± 0.49	6.83 ± 0.66^b	18	3.60 ± 0.4	7.69 ± 1.09^b

^a,^bMeans denoted within the same column with different superscripts are significantly different at (P < 0.05)

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