Fig. 6

The Nrf2-specific blocker ML385 abolished the protective effect of EnSCs on cisplatin-injured KGN cells. A-B Evaluation of cell proliferation ability. The KGN cells were treated for 48 h with ML385 (5 µM or 10 µM) with or without 10 µM cisplatin. Condition medium obtained from the EnSCs was used in the EnSCs group to restore injured KGN cells’ proliferation. Different groups were incubated with condition medium and ML385 (5 µM or 10 µM) for 72 h in accordance with need. C-E Measurement of the protein expression levels of GPX4 and Nrf2. F-I The relative contents of ROS (F), GPx (G), GSSG (H), and MDA (I) were assessed in the coculture model with ML385 addition. The results were shown as mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001, ns indicates no statistical significance)