Fig. 3

Fer-1 reduced the damage caused by cisplatin in KGN cells by inhibiting ferroptosis. A Cell viability was measured by CCK‑8 assay following KGN cells treated with 10 µM cisplatin and different concentrations of Fer-1 for 48 h. Fer-1 recovered KGN cell proliferation ability in a dose-dependent manner. B Cell proliferation capacity was evaluated using EdU labeling assay. C- G Western blotting exhibited Fer-1 restored the protein expression levels of GPX4, Nrf2, SLC7A11 and FTH1. H-K The expression levels of ROS (H), GPx (I), GSSG (J) and MDA (K) in cisplatin-injured KGN cells were changed after Fer-1 addition. Data were shown as mean ± SEM from no less than three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001, * versus control group; ^#P < 0.05, ^##P < 0.01, ^###P< 0.001, ^####P < 0.0001, ^# versus cis (10 µM cisplatin) group)