Fig. 8

The level of endometrial cell apoptosis after hCG intervention. A The mRNA expression levels of Bax and Bcl-2 in ESCs stimulated with hCG for 72 h. B The protein expression levels of Bax and Bcl-2 in endometrial stromal cells after hCG stimulation of ESCs for 72 h. Data were presented as mean ± SD (n = 3): *P < 0.05, **P < 0.01, ***P < 0.001. C ESCs apoptotic cells were detected by flow cytometry after treatment with 0.1 IU/mL of hCG for 72 h. D Immunofluorescence staining was used to determine the localization of Beclin1 and Bcl-2, and DAPI solution for the nucleus staining.Scale: 50 μm. E Co-IP experiment was performed to confirm the effect of hCG on the interactions of Bcl-2 and Beclin1