Fig. 2

The level of endometrial autophagy in RIF patients was down-regulated. A mRNA expression levels of LC3-II and P62 in endometrium of normal control (n = 12) and RIF patients (n = 10). B Left: Western blotting was used to detect the protein levels of LC3B and P62 in the endometrium of normal controls (n = 12) and RIF patients (n = 10). Right: analyzing gels and western blots with Image J. Error bars, mean ± SD. *P < 0.05, **P < 0.01. C Representative IHC images of LC3 localization in endometrial tissue from normal control (n = 12) and RIF patients (n = 10) in secretory phase. Scale, 100 μm and 50 μm, analyzing relative OD with Image J. Error bars, mean ± SD. *P < 0.05, **P < 0.01