Table 2 Primer pairs used for RT-PCR and qPCR
Gene name | Accession number | Orientation | Nucleotide sequence | Nucleotide position | Product Length (bp) | Annealing Temp (℃) |
|---|---|---|---|---|---|---|
Map1a | NM_030995.2 | Sense Anti-Sense | 5’-AAACGGAGGCTGATCAAGGA-3’ 5’-TTCAGGTCTGGCTTGGAGAG-3’ | 1174–1193 1375–1394 | 221 | 60.0 |
Map1b | NM_019217.1 | Sense Anti-Sense | 5’-GGAAGAAGACGGGGAAGACA-3’ 5’-GTCCTCTGCTTTGTCCTCCT-3’ | 2841–2860 3047–3066 | 226 | 60.0 |
Map1s | NM_001106070.1 | Sense Anti-Sense | 5’-CCCTGGAGAAGCAGAGAACA-3’ 5’-GACTCGTCCACCTCAGTTGA-3’ | 1653–1672 1873–1892 | 240 | 60.0 |
Map2 | NM_013066.1 | Sense Anti-Sense | 5’-TCTCAGTTCAGTGCCAGAGG-3’ 5’-TTTCCTGAGGTGCTTCGGAT-3’ | 3051–3070 3252–3271 | 221 | 60.0 |
Map4 | NM_039082634.1 | Sense Anti-Sense | 5’-GGGTGTGTTTTTGAGCAGGT-3’ 5’-GCAGGCTCCAAGCATAGTTC-3’ | 8387–8406 8566–8585 | 199 | 60.0 |
Map6 | NM_017204.1 | Sense Anti-Sense | 5’-CAGCTACAGTGCCCAGTTCA-3’ 5’-TCTGGCTCGTTGAGGTCTTT-3’ | 2026–2045 2179–2198 | 173 | 60.0 |
Map7 | NM_001106270.2 | Sense Anti-Sense | 5’-GAATGGAATTTCAGGCTCCA-3’ 5’-ATGCCACCTCAGGGTATCAG-3’ | 2468–2487 2629–2648 | 181 | 60.0 |
Map9 | NM_001135716.1 | Sense Anti-Sense | 5’-TGATCAAGACGTTGGCAGAG-3’ 5’-GGGCAGAATCTTCGGTTGTA-3’ | 608–627 812–831 | 224 | 60.0 |
Map10 | NM_001277391.1 | Sense Anti-Sense | 5’-ATTTTGCCCTCCTCCTCTGT-3’ 5’-CCCTGCATCTGTGGAAGATT-3’ | 805–824 1007–1026 | 222 | 60.0 |
Map6d1 | NM_001108844.1 | Sense Anti-Sense | 5’-GAAGCCCTCAAGATCCACAA-3’ 5’-GAGGGGCTGATGTCTGAAAG-3’ | 416–435 548–567 | 152 | 60.0 |
Map7d3 | NM_001271327.1 | Sense Anti-Sense | 5’-TCCTGTTGTCAGCTTGGATG-3’ 5’-GGTGCTTGTTTCTTGGTGGT-3’ | 1107–1126 1336–1355 | 249 | 60.0 |
Kif15 | NM_181635.2 | Sense Anti-Sense | 5’-CAGCTTCAGCTGGACAATGC-3’ 5’-TGTTGCGCTCTTTTTCTGCC-3’ | 2547–2566 2741–2760 | 214 | 60.0 |
S16 | NM_001169146.1 | Sense Anti-Sense | 5’-TCCGCTGCAGTCCGTTCAAGTCTT-3’ 5’-GCCAAACTTCTTGGATTCGCAGCG-3’ | 87–110 448–471 | 385 | 60.0 |