Table 1 Antibodies used for different experiments in this report
Antibody (RRID) | Host Species | Vendor | Catalog Number | Working dilution | |
|---|---|---|---|---|---|
IB Analysis | IF/IHC Analysis | ||||
Map1a (AB_2732015) | Rabbit | Abcam | ab184350 | 1:250 | — |
Map1a (AB_10903974) | Rabbit | Abcam | ab101224 | 1:125 | 1:125 (cells) 1:50 (IHC) |
Map1a | Rabbit | Abcam | ab184349 | — | 1:50 (tissue) |
Map2 (AB_2137880) | Rabbit | Proteintech | 17490–1-AP | 1:500 | — |
Occludin (AB_2533977) | Rabbit | Thermo Fisher Scientific | 71–1500 | 1:300 | — |
JAM-A (AB_2533241) | Rabbit | Thermo Fisher Scientific | 36–1700 | 1:500 | 1:100 |
ZO-1 (AB_10733242) | Rabbit | Proteintech | 21773–1-AP | 1:500 | — |
ZO-1 (AB_2533147) | Mouse | Thermo Fisher Scientific | 33–9100 | — | 1:100 |
N-Cadherin (AB_444317) | Rabbit | Abcam | ab18203 | 1:1000 | — |
N-Cadherin (AB_2313779) | Mouse | Thermo Fisher Scientific | 33–3900 |  | 1:100 |
β-Catenin (AB_2533982) | Rabbit | Thermo Fisher Scientific | 71–2700 | 1:500 | 1:100 |
Detyrosinated-α-Tubulin (AB_869990) | Rabbit | Abcam | ab48389 | 1:500 | 1:100 |
Acetylated-α-Tubulin (AB_448182) | Rabbit | Abcam | ab24610 | 1:500 | 1:100 |
Tyrosinated-α-Tubulin (AB_261811) | Mouse | Sigma-Aldrich | T9028 | 1:500 | 1:100 |
Dynein1 (Heavy chain, Dync1h1) | Mouse | Santa Cruz | sc-514579 | 1:250 | — |
Dynein1 (Heavy chain, Dync1h1) (AB_2261765) | Rabbit | Proteintech | 12345–1-AP | — | 1:100 |
Kif15 (AB_11182836) | Rabbit | Proteintech | 55407–1-AP | 1:500 | 1:100 |
EB1 (AB_397891) | Mouse | BD Biosciences | 610534 | 1:250 | 1:100 |
CAMSAP2 (AB_2068826) | Rabbit | Proteintech | 17880–1-AP | 1:500 | 1:100 |
α-Tubulin (AB_2241126) | Rabbit | Abcam | Ab7291 | 1:500 | 1:100 |
β-Tubulin (AB_2210370) | Rabbit | Abcam | Ab6046 | 1:500 | 1:100 |
Arp3 (AB_476749) | Mouse | Sigma-Aldrich | A5979 | 1:500 | 1:100 |
Eps8 (AB_397544) | Mouse | BD Biosciences | 610143 | 1:500 | 1:100 |
Palladin (AB_2158782) | Rabbit | Proteintech | 10853–1-AP | 1:250 | — |
Annexin A2 (AB_11129437) | Rabbit | Cell Signaling Technology | 8235 | 1:500 | — |
Vimentin (AB_628437) | Mouse | Santa Cruz | sc-6260 | 1:500 | 1:100 |
Septin7 (AB_2254298) | Rabbit | Proteintech | 10818–1-AP | 1:250 | 1:100 |
GAPDH (AB_2107436) | Mouse | Proteintech | 60004–1-Ig | 1:3000 | — |
β-actin (AB_630836) | Mouse | Santa Cruz Biotechnology | sc-4778 | 1:1000 | — |
Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) | Mouse | Abcam | ab175775 | 1:3000 | — |
Goat Anti-Rabbit IgG H&L (IRDye® 800) | Rabbit | Abcam | ab216773 | 1:3000 | — |
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) | Rabbit | Abcam | ab175773 | 1:3000 | — |
Goat Anti-Mouse IgG H&L (IRDye® 800) | Mouse | Abcam | ab216772 | 1:3000 | — |
Rhodamine Phalloidin (Molecular Probes™) |  | Thermo Fisher Scientific | R415 | — | 1:200 |
Mouse IgG Alexa Fluor 488 (AB_2534088) | Goat | Thermo Fisher Scientific | A-11029 | — | 1:250 |
Mouse IgG Alexa Fluor 555 (AB_141780) | Goat | Thermo Fisher Scientific | A-21424 | — | 1:250 |
Rabbit IgG Alexa Fluor 488 (AB_2576217) | Goat | Thermo Fisher Scientific | A-11034 | — | 1:250 |
Rabbit IgG Alexa Fluor 555 (AB_2535850) | Goat | Thermo Fisher Scientific | A-21429 | — | 1:250 |