Fig. 8
CdCl2 down-regulates expression of microtubule-associated proteins (Maps) in testes of adult rats. A Adult rats at 250–280 gm b.w. in groups of n = 3 rats were treated with a single dose of CdCl2 (3 mg/kg b.w., by intraperitoneal, i.p., administration) as described [33, 73, 74] at time 0 h (control, Ctrl), and then terminated at specified time points at 6, 12, 24, 48, 72, and 96 h. One testis was used for histological and IF analyses, and the other testis for IB. As such, each reported experiment had n = 3 testes from different rats for analysis. Lysates were obtained from these rats for IB analysis. A time-dependent down-regulation of Map1a and Kif15 was noted, wherein GAPDH served as a protein loading control. Results of a representation experiment were shown from n = 3 independent experiments. B Results of composite data with each bar representing a mean ± SD of n = 3 experiments on two Maps namely Map1a and Kif15 (a microtubule-dependent plus-end directed motor protein that moves cargoes towards the plus ( +)-end of the polarized MT protofilaments. **, P <0.01; ***, P < 0.005 by paired Student's t-test; ns, non-significantly