Fig. 2

Stage-specific and spatiotemporal expression of Map1a across the seminiferous epithelium in adult rat testes during the epithelial cycle. A Map1a (green fluorescence) co-localized with microtubules (visualized by α-tubulin staining, red fluorescence), displaying stage-specific and spatiotemporal expression across the seminiferous epithelium. The right panel shows the results of a negative control when the primary antibody was substituted by using IgG of the corresponding animal species, with no detectable green fluorescence of Map1a was noted. Scale bar, 300 µm (left panel); 30 µm for the enlarged image; 100 µm in the right panel (control groups) to demonstrate no staining in negative control group. B Stage-specific expression and distribution at the apical ES with schematic drawing at the right panel. Scale bar, 20 µm, which applies to other images. C Co-localization of Map1a with basal ES (N-cadherin) and TJ (ZO-1) proteins at the BTB. Scale bar, 20 µm