Fig. 3
GCs dysfunction along with the disrupted GCs differentiation in PCOS. A Heatmap showing the core gene in modules regulating GCs differentiation in Monocle 3 (resolution = 0.0001). B The top 5 enriched GO (biological pathway) terms of downregulated DEGs in module 4. C The top 5 enriched GO (biological pathway) terms of upregulated DEGs in module 3/14/15. D Monocle 3 generated pseudotemporal trajectory of preantral GCs, small antral GCs, KRT8high mural GCs and HSPA6high mural GCs. E The granulosa cell trajectory predicted by Monocle 3 and visualized by UMAP. Cells were ordered in pseudotime colored in a gradient from purple to yellow. F Top 15 significant switching genes were ordered in pseudotime in the trajectory, from preantral GCs, small antral GCs to KRT8high mural GCs. G Top 15 significant switching genes were ordered in pseudotime in the trajectory, from preantral GCs, small antral GCs to HSPA6high mural GCs. H Venn plot of all the significant switching genes in above two trajectories. I Pathway enrichment of the 47 significant switching genes specific in KRT8high mural GCs differentiation trajectory