Fig. 3

miR-124-3p inhibits migration and proliferation, inducing apoptosis on uterine epithelial cells in vitro. a Real-time PCR analysis for miR-124-3p expression in Ishikawa cells after miR-124-3p transfection with mimics and inhibitor. b, c Phase-contrast images of wound-healing assays to test the effect of miR-124-3p on cell migration. Data are presented as the size of surface area of Ishikawa cells entering the migration zone. d CCK-8 assay was used to evaluate cell proliferation capabilities of Ishikawa cells after transfection with miR-124-3p mimics and inhibitor. e Proportion of apoptotic cells was evaluated by Annexin V staining followed by flow cytometry analysis after miR-124-3p mimics and inhibitor transfection. All data represent the mean ± SD of three independent experiments. Different superscripts indicate significance at p < 0.05 (one-way ANOVA with Duncan’s test)