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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Inhibition of mitochondrial cyclophilin D, a downstream target of glycogen synthase kinase 3α, improves sperm motility

Fig. 1

Change in CypD in cauda epididymal spermatozoa after capacitation. A Representative western blot images of changes in CypD and ATP5A proteins in spermatozoa after incubation in TCM for 1 h. CypD but not ATP5A levels were markedly decreased under capacitation condition. At least three independent experiments were subjected to western blot analysis. B Representative images of immunolocalization of CypD and ATP5A in cauda epididymal spermatozoa. Immunoreactivity of CypD was found in the midpiece and was decreased after incubation in TCM for 1 h. Rabbit and mouse IgG were used as a negative control of ATP5A and CypD antibodies, respectively. Sperm head, midpiece, and principal piece of the tail are annotated by asterisk, arrows, and arrowheads, respectively. Nuclei were stained blue by DAPI. At least three independent experiments were subjected to immunocytochemistry. AU, arbitrary units. Bar, 10 μm

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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