Fig. 6
From: NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes
NET1 protects RAC1 from HACE1-mediated degradation. (A) Rac1 mRNA expression levels in Net1-KD and control oocytes (n = 50 per group). (B) RAC1 protein levels in the control, Net1-KD, and Net1-KD + MG132 oocytes. The immunoblots were probed with NET1, RAC1, and GAPDH antibodies. (C) Relative- intensity results for NET1 protein expression in the control, Net1-KD, and Net1-KD + MG132 oocytes. (D) Relative-intensity results for RAC1 protein expression in the control, control + MG132, Net1-KD, and Net1-KD + MG132 oocytes. (E) RAC1 protein levels in the control, Net1-KD, Net1-KD + Hace1-KD oocytes. The immunoblots were probed with NET1, RAC1, and GAPDH antibodies