Fig. 2

NET1 guarantees meiotic progression in mouse oocytes. (A) KD of endogenous NET1 protein after Net1-siRNA injection was confirmed by western blot analysis. (B) Relative intensity results for the NET1 protein expression in control and Net1-KD oocytes. (C) Representative images of oocytes from control and Net1-KD oocytes. Red arrowheads denote oocytes with apparent symmetrical division and blue arrowheads indicate oocytes that fail to extrude polar bodies (scale bar, 80 μm). (D, E). Quantitative analysis of GVBD and Pb1 extrusion rates between control and Net1-KD oocytes (GVBD, 81.7 ± 1.64% vs. 81.4 ± 3.14% ; Pb1, 77.5 ± 1.41% vs. 31.2 ± 2.48%). (F). The percentage of oocytes with large polar bodies after Net1-siRNA injection (8.7 ± 1.55% vs. 28.9 ± 2.5%). The Figure depicts the mean percentage ± SD of the results obtained from three independent experiments (*significantly different at P < 0.05, **significantly different at P < 0.01)