Fig. 1
From: NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes
Subcellular localization and expression of NET1 during oocyte maturation. (A) Expression of NET1 during meiotic maturation at GV, GVBD, MI, and MII stages (the molecular mass of NET1 is 64 kDa). Proteins from 100 oocytes were loaded for each sample. (B) Relative intensity results for NET1 protein expression in GV, GVBD, MI, and MII oocytes. (C) Confocal microscopy showing immunostaining for NET1 (green) and DNA (red) in oocytes at GV, GVBD, MI, and MII stages (scale bar = 20 μm). (D) Double-labeling of metaphase oocytes with NET1 (red) and α-tubulin antibody (green), and counterstaining of DNA with Hoechst 33,342 (blue) (scale bars, 20 μm)